AT3G29010

lipoate-protein ligase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009773
Physical Location & Seq
Forward (+)
11006564 .. 11008216
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G29010.2

Sequence Viewer

Length: 789 bp
ATGAGTTTAAGTAGAGCAAAGGATTTGAGACTTCCGTTGATGAATTTGGTTAGACTTAAAGGTACACCAATTTTGGAGCAACTTCATTTAGAAGAAAGGCTTCTTAGAACATCTTCTGATAATTGGTGCATTGTTAATGATGGAACCAATGTTCCTACCATTGTGATGGGAATGTCAGGGAAGCCTTCTCAGCTTCTTGAGGTTGGACCAGTTATGGAAGATCGAATACCGGTGATTAAACGATTTACGGGAGGAGGAACTGTGATTGTGGATAAGAGTACTTTGTTTGTGAGTTTGATTTGTAACAAGGATGATGTACCAAATGTTCAGCCTTATCCTCGTTCTGTTATGGCTTGGAGTGGTTCGTTGTATGATGAAGTGTTTAAGAGTGTTAATGGTTTCCAACTACGTGAGAATGATTATGTGTTTGGTGATCGTAAGTTCGGTGGGAATGCTCAATCGATAATTAAGAATAGATGGATTCACCATACTTCATTTCTATGGGACTATGATGTGAGAAACATGGCTTACCTTAAGCTGCCTTCGAGAGTGCCACAGTACCGGTTGGAAAGAGATCACACAGATTTCGTGTGTAGAATGAAGGATTACATTGAAAGATCAGATTTTGTTGAGAAGACAGTAAAAGCTGTAAGGAACCAATTCACATTGAAGCAAGTGAACCTTGAAGATATTGATTCCTATGCGAAAGGAGGATATTTGAAGACTACGAGGCTTCTTACGATGGAAGAACTCGAAGAAGCAATGGCTGATACCATTCAAAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

30.16

Weight (kDa)

7.69

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 2 - 150 2e-20 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 4 cut(s) 64, 280, 318, 560
AflII CTTAAG 1 cut(s) 533
AgeI ACCGGT 2 cut(s) 229, 561
AgsI TTSAA 5 cut(s) 614, 670, 686, 721, 779
AloI GAACNNNNNNTCC 4 cut(s) 135, 136, 167, 168
AluBI AGCT 3 cut(s) 193, 538, 647
AluI AGCT 3 cut(s) 193, 538, 647
Alw26I GTCTC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 538
ApoI RAATTY 1 cut(s) 43
AsiGI ACCGGT 2 cut(s) 229, 561
Asp700I GAANNNNTTC 3 cut(s) 99, 112, 659
AspS9I GGNCC 1 cut(s) 206
AsuHPI GGTGA 3 cut(s) 244, 443, 476
AvaII GGWCC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
BbsI GAAGAC 2 cut(s) 641, 728
BbvI GCAGC 1 cut(s) 525
BccI CCATC 4 cut(s) 134, 160, 471, 736
BcoDI GTCTC 1 cut(s) 22
BfrI CTTAAG 1 cut(s) 533
BisI GCNGC 1 cut(s) 539
BlsI GCNGC 1 cut(s) 540
BmcAI AGTACT 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 145, 656
BpiI GAAGAC 2 cut(s) 641, 728
BpuEI CTTGAG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 461
BsaAI YACGTR 1 cut(s) 410
BsaWI WCCGGW 2 cut(s) 229, 561
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bse118I RCCGGY 2 cut(s) 229, 561
Bse1I ACTGG 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 768
BseCI ATCGAT 1 cut(s) 461
BseGI GGATG 1 cut(s) 316
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 209
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 525
BshTI ACCGGT 2 cut(s) 229, 561
BshVI ATCGAT 1 cut(s) 461
BsiSI CCGG 2 cut(s) 230, 562
BslFI GGGAC 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 457
Bsp143I GATC 4 cut(s) 220, 433, 574, 617
BspCNI CTCAG 1 cut(s) 202
BspDI ATCGAT 1 cut(s) 461
BspLI GGNNCC 2 cut(s) 145, 656
BspTI CTTAAG 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 768
BsrFI RCCGGY 2 cut(s) 229, 561
BsrI ACTGG 1 cut(s) 209
BssAI RCCGGY 2 cut(s) 229, 561
BssMI GATC 4 cut(s) 220, 433, 574, 617
Bst4CI ACNGT 3 cut(s) 262, 558, 640
BstAFI CTTAAG 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 104, 189
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 4 cut(s) 223, 436, 577, 620
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 4 cut(s) 220, 433, 574, 617
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 525
BstV2I GAAGAC 2 cut(s) 641, 728
BstXI CCANNNNNNTGG 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 461
BsuTUI ATCGAT 1 cut(s) 461
BtsCI GGATG 1 cut(s) 316
Cfr10I RCCGGY 2 cut(s) 229, 561
Cfr13I GGNCC 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 461
Csp6I GTAC 4 cut(s) 63, 279, 317, 559
CspAI ACCGGT 2 cut(s) 229, 561
CviAII CATG 2 cut(s) 523, 786
CviQI GTAC 4 cut(s) 63, 279, 317, 559
DdeI CTNAG 2 cut(s) 104, 189
DpnI GATC 4 cut(s) 222, 435, 576, 619
DpnII GATC 4 cut(s) 220, 433, 574, 617
Eco47I GGWCC 1 cut(s) 206
FaeI CATG 2 cut(s) 526, 789
FalI AAGNNNNNCTT 4 cut(s) 84, 116, 666, 698
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 522, 785
Fnu4HI GCNGC 1 cut(s) 539
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 539
GluI GCNGC 1 cut(s) 539
HapII CCGG 2 cut(s) 230, 562
Hin1II CATG 2 cut(s) 526, 789
HinfI GANTC 2 cut(s) 481, 695
HpaII CCGG 2 cut(s) 230, 562
HphI GGTGA 3 cut(s) 244, 443, 476
Hpy166II GTNNAC 2 cut(s) 65, 679
Hpy188I TCNGA 2 cut(s) 118, 622
Hpy188III TCNNGA 2 cut(s) 197, 546
Hpy8I GTNNAC 2 cut(s) 65, 679
HpyAV CCTTC 3 cut(s) 195, 552, 595
HpyCH4III ACNGT 3 cut(s) 262, 558, 640
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 104, 189
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 2 cut(s) 526, 789
Kzo9I GATC 4 cut(s) 220, 433, 574, 617
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 4 cut(s) 162, 222, 243, 575
Lsp1109I GCAGC 1 cut(s) 525
MaeII ACGT 1 cut(s) 409
MaeIII GTNAC 1 cut(s) 302
MalI GATC 4 cut(s) 222, 435, 576, 619
MboI GATC 4 cut(s) 220, 433, 574, 617
MboII GAAGA 8 cut(s) 104, 105, 230, 646, 698, 733, 758, 767
MluCI AATT 5 cut(s) 43, 69, 121, 465, 659
MmeI TCCRAC 3 cut(s) 184, 427, 546
MnlI CCTC 6 cut(s) 193, 245, 248, 348, 704, 723
MroXI GAANNNNTTC 3 cut(s) 99, 112, 659
MseI TTAA 8 cut(s) 8, 57, 135, 237, 384, 393, 468, 534
MslI CAYNNNNRTG 2 cut(s) 164, 499
MspCI CTTAAG 1 cut(s) 533
MspI CCGG 2 cut(s) 230, 562
Mva1269I GAATGC 1 cut(s) 457
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeII GATC 4 cut(s) 220, 433, 574, 617
NlaIII CATG 2 cut(s) 526, 789
NlaIV GGNNCC 2 cut(s) 145, 656
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 3 cut(s) 99, 112, 659
PfeI GAWTC 2 cut(s) 481, 695
PinAI ACCGGT 2 cut(s) 229, 561
PkrI GCNGC 1 cut(s) 540
Ppu21I YACGTR 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 145, 656
PspPI GGNCC 1 cut(s) 206
PsrI GAACNNNNNNTAC 2 cut(s) 309, 341
RsaI GTAC 4 cut(s) 64, 280, 318, 560
RsaNI GTAC 4 cut(s) 63, 279, 317, 559
RseI CAYNNNNRTG 2 cut(s) 164, 499
SaqAI TTAA 8 cut(s) 8, 57, 135, 237, 384, 393, 468, 534
SatI GCNGC 1 cut(s) 539
Sau3AI GATC 4 cut(s) 220, 433, 574, 617
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 1 cut(s) 280
SetI ASST 8 cut(s) 64, 195, 204, 412, 534, 540, 649, 684
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 164, 499
SmlI CTYRAG 2 cut(s) 197, 533
SmoI CTYRAG 2 cut(s) 197, 533
Sse9I AATT 5 cut(s) 43, 69, 121, 465, 659
TaaI ACNGT 3 cut(s) 262, 558, 640
TaiI ACGT 1 cut(s) 412
TaqI TCGA 4 cut(s) 223, 461, 545, 753
TasI AATT 5 cut(s) 43, 69, 121, 465, 659
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 2 cut(s) 481, 695
Tru1I TTAA 8 cut(s) 8, 57, 135, 237, 384, 393, 468, 534
Tru9I TTAA 8 cut(s) 8, 57, 135, 237, 384, 393, 468, 534
TseI GCWGC 1 cut(s) 538
TspDTI ATGAA 5 cut(s) 56, 74, 390, 483, 614
TspGWI ACGGA 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 43
XmnI GAANNNNTTC 3 cut(s) 99, 112, 659
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.