FvH4_7g07490

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009773
Physical Location & Seq
Reverse (-)
7388558 .. 7391824
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07490.t2

Sequence Viewer

Length: 801 bp
ATGACTATACCTCAAACAAGAAATGTTGGGCTTCCATTGATGAGGCTGATCAGACTAGAAGGAATACCCATCCTGCAGCAGCTTCATTTAGAGGAGCAACTGCTTCGAACCTCGTCTGATAATTGGTGCATTATAAATGATGGTACCAATATTCCTAACATTGTCATGGGTATTTCAGGAAAACCTGCAGAGCTTCTTGACATTGATCTGGTGTTACGAGATCAGGTTCCTGTCATCAAAAGATTTACTGGAGGTGGCACGGTTACAGTTGATCATGGCACGATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTGGCTTGCAACTGTATCCGCGACCTATAATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAAGATTTGGCGATTTTCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAATCGATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGCACCTGAATATCGATCGGCAAGGGACCATTTGGAATTTATATGCCCCATGAAGGACTATATGCCAAGATCAGTTTTCATCGACAAAACTGTCAAGGCAATTAGTACCGAATTTTCAGTATATTCTGAACAAACAGACCCATTCAAAATCAGCTCAAACTTTGAGCCCTCAACTAGACTACTTACAAGGCAGGAGTTGGAAGAAGTAGCCTCTCAACCTGAGAGCCCTGTGTCTCAGTCTTTGTCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.63

Weight (kDa)

7.75

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 24 - 221 8e-30 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
AasI GACNNNNNNGTC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 193
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 2 cut(s) 143, 550
AccII CGCG 1 cut(s) 340
AciI CCGC 2 cut(s) 338, 436
AclWI GGATC 2 cut(s) 475, 488
AcsI RAATTY 2 cut(s) 584, 659
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 2 cut(s) 145, 655
AfiI CCNNNNNNNGG 2 cut(s) 501, 601
AgeI ACCGGT 1 cut(s) 474
AgsI TTSAA 1 cut(s) 694
AjnI CCWGG 2 cut(s) 319, 353
AleI CACNNNNGTG 1 cut(s) 408
AluBI AGCT 4 cut(s) 82, 193, 538, 702
AluI AGCT 4 cut(s) 82, 193, 538, 702
Alw26I GTCTC 1 cut(s) 786
AlwI GGATC 2 cut(s) 475, 488
ApeKI GCWGC 2 cut(s) 76, 79
ApoI RAATTY 2 cut(s) 584, 659
AsiGI ACCGGT 1 cut(s) 474
Asp718I GGTACC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 574
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 106
AvaII GGWCC 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 553
BamHI GGATCC 1 cut(s) 480
BanI GGYRCC 2 cut(s) 143, 550
BanII GRGCYC 2 cut(s) 717, 776
BbvI GCAGC 2 cut(s) 88, 91
BccI CCATC 2 cut(s) 77, 134
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 321, 355
BciVI GTATCC 1 cut(s) 345
BclI TGATCA 2 cut(s) 48, 271
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 2 cut(s) 56, 723
BfmI CTRYAG 2 cut(s) 74, 186
BfuAI ACCTGC 1 cut(s) 193
BfuI GTATCC 1 cut(s) 345
BisI GCNGC 2 cut(s) 77, 80
BlsI GCNGC 2 cut(s) 78, 81
Bme1390I CCNGG 2 cut(s) 321, 355
Bme18I GGWCC 1 cut(s) 574
BmgT120I GGNCC 1 cut(s) 574
BmiI GGNNCC 5 cut(s) 145, 228, 482, 552, 575
BmrFI CCNGG 2 cut(s) 321, 355
BmsI GCATC 1 cut(s) 301
BpmI CTGGAG 2 cut(s) 270, 376
Bpu14I TTCGAA 1 cut(s) 106
Bsa29I ATCGAT 2 cut(s) 461, 562
BsaWI WCCGGW 1 cut(s) 474
Bsc4I CCNNNNNNNGG 2 cut(s) 501, 601
Bse118I RCCGGY 1 cut(s) 474
Bse1I ACTGG 1 cut(s) 253
BseBI CCWGG 2 cut(s) 321, 355
BseCI ATCGAT 2 cut(s) 461, 562
BseGI GGATG 3 cut(s) 69, 316, 491
BseLI CCNNNNNNNGG 2 cut(s) 501, 601
BseMII CTCAG 2 cut(s) 759, 797
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 2 cut(s) 88, 91
Bsh1236I CGCG 1 cut(s) 340
Bsh1285I CGRYCG 1 cut(s) 566
BshNI GGYRCC 2 cut(s) 143, 550
BshTI ACCGGT 1 cut(s) 474
BshVI ATCGAT 2 cut(s) 461, 562
BsiEI CGRYCG 1 cut(s) 566
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 2 cut(s) 518, 587
BslI CCNNNNNNNGG 2 cut(s) 501, 601
BsmAI GTCTC 1 cut(s) 786
BsmFI GGGAC 2 cut(s) 518, 587
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 3 cut(s) 553, 717, 776
Bsp143I GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
BspACI CCGC 2 cut(s) 338, 436
BspCNI CTCAG 2 cut(s) 760, 796
BspDI ATCGAT 2 cut(s) 461, 562
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 5 cut(s) 145, 228, 482, 552, 575
BspMAI CTGCAG 2 cut(s) 78, 190
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 2 cut(s) 475, 488
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 2 cut(s) 143, 550
BsrFI RCCGGY 1 cut(s) 474
BsrI ACTGG 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 474
BssMI GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
Bst2UI CCWGG 2 cut(s) 321, 355
Bst4CI ACNGT 4 cut(s) 262, 268, 333, 640
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 768, 783
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 3 cut(s) 69, 316, 491
BstFNI CGCG 1 cut(s) 340
BstKTI GATC 7 cut(s) 51, 208, 223, 274, 483, 566, 620
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
BstMCI CGRYCG 1 cut(s) 566
BstNI CCWGG 2 cut(s) 321, 355
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 319, 353
BstSFI CTRYAG 2 cut(s) 74, 186
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 88, 91
BstX2I RGATCY 1 cut(s) 480
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 480
Bsu15I ATCGAT 2 cut(s) 461, 562
BsuI GTATCC 1 cut(s) 345
BsuTUI ATCGAT 2 cut(s) 461, 562
BtsCI GGATG 3 cut(s) 69, 316, 491
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 326
Cfr10I RCCGGY 1 cut(s) 474
Cfr13I GGNCC 1 cut(s) 574
ClaI ATCGAT 2 cut(s) 461, 562
Csp6I GTAC 2 cut(s) 144, 654
CspAI ACCGGT 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 5 cut(s) 166, 275, 523, 598, 796
CviQI GTAC 2 cut(s) 144, 654
DdeI CTNAG 2 cut(s) 768, 783
DpnI GATC 7 cut(s) 50, 207, 222, 273, 482, 565, 619
DpnII GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
Eco24I GRGCYC 2 cut(s) 717, 776
Eco47I GGWCC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 554
Eco91I GGTNACC 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 319, 353
EcoT38I GRGCYC 2 cut(s) 717, 776
FaeI CATG 5 cut(s) 169, 278, 526, 601, 799
FaqI GGGAC 2 cut(s) 518, 587
FatI CATG 5 cut(s) 165, 274, 522, 597, 795
FauNDI CATATG 1 cut(s) 299
FbaI TGATCA 2 cut(s) 48, 271
Fnu4HI GCNGC 2 cut(s) 77, 80
FokI GGATG 3 cut(s) 56, 323, 478
FriOI GRGCYC 2 cut(s) 717, 776
Fsp4HI GCNGC 2 cut(s) 77, 80
FspBI CTAG 2 cut(s) 56, 723
GluI GCNGC 2 cut(s) 77, 80
GsuI CTGGAG 2 cut(s) 270, 376
HapII CCGG 1 cut(s) 475
Hin1II CATG 5 cut(s) 169, 278, 526, 601, 799
HindIII AAGCTT 1 cut(s) 536
HpaII CCGG 1 cut(s) 475
HphI GGTGA 1 cut(s) 395
Hpy188I TCNGA 3 cut(s) 53, 118, 676
Hpy188III TCNNGA 3 cut(s) 177, 197, 410
HpyAV CCTTC 3 cut(s) 53, 416, 595
HpyCH4III ACNGT 4 cut(s) 262, 268, 333, 640
HpyCH4V TGCA 5 cut(s) 76, 129, 188, 303, 328
HpyF3I CTNAG 2 cut(s) 768, 783
Hsp92II CATG 5 cut(s) 169, 278, 526, 601, 799
KpnI GGTACC 1 cut(s) 147
Ksp22I TGATCA 2 cut(s) 48, 271
Kzo9I GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 2 cut(s) 88, 91
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 56, 723
MaeIII GTNAC 4 cut(s) 213, 262, 289, 431
MalI GATC 7 cut(s) 50, 207, 222, 273, 482, 565, 619
MboI GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
MboII GAAGA 1 cut(s) 761
MflI RGATCY 1 cut(s) 480
MhlI GDGCHC 3 cut(s) 553, 717, 776
MluCI AATT 5 cut(s) 121, 365, 584, 648, 659
MmeI TCCRAC 1 cut(s) 726
MnlI CCTC 8 cut(s) 21, 36, 85, 121, 245, 505, 727, 769
MslI CAYNNNNRTG 2 cut(s) 164, 408
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 2 cut(s) 321, 355
MvaI CCWGG 2 cut(s) 321, 355
MvnI CGCG 1 cut(s) 340
NdeI CATATG 1 cut(s) 299
NdeII GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
NlaIII CATG 5 cut(s) 169, 278, 526, 601, 799
NlaIV GGNNCC 5 cut(s) 145, 228, 482, 552, 575
NmuCI GTSAC 1 cut(s) 289
NspV TTCGAA 1 cut(s) 106
OliI CACNNNNGTG 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 474
PkrI GCNGC 2 cut(s) 78, 81
Ple19I CGATCG 1 cut(s) 566
PsiI TTATAA 1 cut(s) 134
Psp6I CCWGG 2 cut(s) 319, 353
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 2 cut(s) 319, 353
PspN4I GGNNCC 5 cut(s) 145, 228, 482, 552, 575
PspPI GGNCC 1 cut(s) 574
PsrI GAACNNNNNNTAC 2 cut(s) 512, 544
PstI CTGCAG 2 cut(s) 78, 190
PsuI RGATCY 1 cut(s) 480
PvuI CGATCG 1 cut(s) 566
RsaI GTAC 2 cut(s) 145, 655
RsaNI GTAC 2 cut(s) 144, 654
RseI CAYNNNNRTG 2 cut(s) 164, 408
SatI GCNGC 2 cut(s) 77, 80
Sau3AI GATC 7 cut(s) 48, 205, 220, 271, 480, 563, 617
Sau96I GGNCC 1 cut(s) 574
ScrFI CCNGG 2 cut(s) 321, 355
SduI GDGCHC 3 cut(s) 553, 717, 776
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 2 cut(s) 74, 186
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 1 cut(s) 574
SmiMI CAYNNNNRTG 2 cut(s) 164, 408
Sse9I AATT 5 cut(s) 121, 365, 584, 648, 659
SsiI CCGC 2 cut(s) 338, 436
SspI AATATT 1 cut(s) 151
SspMI CTAG 2 cut(s) 56, 723
StyD4I CCNGG 2 cut(s) 319, 353
TaaI ACNGT 4 cut(s) 262, 268, 333, 640
TaqI TCGA 4 cut(s) 106, 461, 562, 630
TasI AATT 5 cut(s) 121, 365, 584, 648, 659
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 76, 79
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 4 cut(s) 74, 286, 614, 616
VpaK11BI GGWCC 1 cut(s) 574
XapI RAATTY 2 cut(s) 584, 659
XspI CTAG 2 cut(s) 56, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.