Rw7G003710

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009773
Physical Location & Seq
Forward (+)
3015747 .. 3017899
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003710.1

Sequence Viewer

Length: 744 bp
ATGATACTACTTGGTCAGATTTTCACTATTGTTTATACATGCATTAACCAAGTTTGTTGTGTTCTTTTATTCACTTTTTGTATTGGAATTTATAAAGGGTTCCGTATTCTGCGTAGAAAGCCTGCAGAGCTTCTTGACGTTGATCTGGTGTTACGAGATCAGGTTCCTGTCATTAAAAGGTTTACTGGAGGTGGCACGGTTACAGTTGATAATGGCACGATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTTGCTTGCAACTATATCCGCGACCTATAATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAGGATTTGGTGACTTTCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAATCTATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGTTCCTGAATATCGATTGGCAAGGGACCATTTGGAATTCATATGCCGCATGAAAGACTACATGCCAAGATCAGTTTTCATCGACAAAACCGTCGAGGCAATCAGTACCGAATTTTCAGTCAAATCTGAACAAACAGAACCATTCAAAACCAACTCAAACTTTGTGCCCTCAACTAGACTATTGACAAGGCAAGAGTTGGAAGAAGTAGCTTTGGGCTCTCAACCTGAGAGCCCTCTCTCTGAGTCCCTGTCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

28.62

Weight (kDa)

8.82

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 40 - 200 1.1e-22 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 93
AasI GACNNNNNNGTC 1 cut(s) 575
AccII CGCG 1 cut(s) 277
AciI CCGC 3 cut(s) 275, 373, 532
AclWI GGATC 2 cut(s) 412, 425
AcsI RAATTY 3 cut(s) 87, 521, 596
AcuI CTGAAG 1 cut(s) 491
AfaI GTAC 1 cut(s) 592
AfiI CCNNNNNNNGG 1 cut(s) 438
AgeI ACCGGT 1 cut(s) 411
AgsI TTSAA 1 cut(s) 631
AjnI CCWGG 1 cut(s) 290
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 3 cut(s) 130, 475, 695
AluI AGCT 3 cut(s) 130, 475, 695
AlwI GGATC 2 cut(s) 412, 425
ApoI RAATTY 3 cut(s) 87, 521, 596
ArsI GACNNNNNNTTYG 2 cut(s) 588, 620
AsiGI ACCGGT 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 511
AsuHPI GGTGA 2 cut(s) 332, 344
AvaII GGWCC 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 654
BamHI GGATCC 1 cut(s) 417
BanII GRGCYC 2 cut(s) 704, 719
BciT130I CCWGG 1 cut(s) 292
BfaI CTAG 1 cut(s) 660
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 1 cut(s) 532
BlsI GCNGC 1 cut(s) 533
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 5 cut(s) 101, 165, 419, 489, 512
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 238
BpmI CTGGAG 2 cut(s) 207, 313
Bsa29I ATCGAT 1 cut(s) 499
BsaJI CCNNGG 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 411
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse118I RCCGGY 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 190
BseBI CCWGG 1 cut(s) 292
BseCI ATCGAT 1 cut(s) 499
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 2 cut(s) 253, 428
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 702, 717
BseNI ACTGG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 277
BshTI ACCGGT 1 cut(s) 411
BshVI ATCGAT 1 cut(s) 499
BsiSI CCGG 1 cut(s) 412
BslFI GGGAC 3 cut(s) 455, 524, 715
BslI CCNNNNNNNGG 1 cut(s) 438
BsmFI GGGAC 3 cut(s) 455, 524, 715
Bsp1286I GDGCHC 3 cut(s) 654, 704, 719
Bsp143I GATC 4 cut(s) 142, 157, 417, 554
BspACI CCGC 3 cut(s) 275, 373, 532
BspCNI CTCAG 2 cut(s) 703, 718
BspDI ATCGAT 1 cut(s) 499
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 5 cut(s) 101, 165, 419, 489, 512
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 2 cut(s) 412, 425
BsrFI RCCGGY 1 cut(s) 411
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 4 cut(s) 142, 157, 417, 554
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 3 cut(s) 199, 205, 577
BstC8I GCNNGC 2 cut(s) 123, 263
BstDEI CTNAG 2 cut(s) 711, 726
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 2 cut(s) 253, 428
BstFNI CGCG 1 cut(s) 277
BstKTI GATC 4 cut(s) 145, 160, 420, 557
BstMBI GATC 4 cut(s) 142, 157, 417, 554
BstMWI GCNNNNNNNGC 2 cut(s) 118, 127
BstNI CCWGG 1 cut(s) 292
BstNSI RCATGY 2 cut(s) 42, 550
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 290
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 654
BstUI CGCG 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 417
BstXI CCANNNNNNTGG 1 cut(s) 329
BstYI RGATCY 1 cut(s) 417
Bsu15I ATCGAT 1 cut(s) 499
BsuTUI ATCGAT 1 cut(s) 499
BtsCI GGATG 2 cut(s) 253, 428
Cac8I GCNNGC 2 cut(s) 123, 263
Cfr10I RCCGGY 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 511
ClaI ATCGAT 1 cut(s) 499
Csp6I GTAC 1 cut(s) 591
CspAI ACCGGT 1 cut(s) 411
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 4 cut(s) 39, 460, 535, 547
CviJI RGCY 7 cut(s) 121, 130, 300, 475, 695, 702, 717
CviKI_1 RGCY 7 cut(s) 121, 130, 300, 475, 695, 702, 717
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 2 cut(s) 711, 726
DpnI GATC 4 cut(s) 144, 159, 419, 556
DpnII GATC 4 cut(s) 142, 157, 417, 554
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 322
Eco24I GRGCYC 2 cut(s) 704, 719
Eco47I GGWCC 1 cut(s) 511
Eco57I CTGAAG 1 cut(s) 491
Eco91I GGTNACC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 44
EcoT38I GRGCYC 2 cut(s) 704, 719
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 4 cut(s) 42, 463, 538, 550
FaqI GGGAC 3 cut(s) 455, 524, 715
FatI CATG 4 cut(s) 38, 459, 534, 546
FauNDI CATATG 2 cut(s) 236, 527
Fnu4HI GCNGC 1 cut(s) 532
FokI GGATG 2 cut(s) 260, 415
FriOI GRGCYC 2 cut(s) 704, 719
Fsp4HI GCNGC 1 cut(s) 532
FspBI CTAG 1 cut(s) 660
GluI GCNGC 1 cut(s) 532
GsuI CTGGAG 2 cut(s) 207, 313
HapII CCGG 1 cut(s) 412
Hin1II CATG 4 cut(s) 42, 463, 538, 550
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 1 cut(s) 728
HpaII CCGG 1 cut(s) 412
HphI GGTGA 2 cut(s) 332, 344
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 3 cut(s) 18, 613, 727
Hpy188III TCNNGA 3 cut(s) 134, 347, 491
Hpy8I GTNNAC 1 cut(s) 183
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 3 cut(s) 199, 205, 577
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 4 cut(s) 42, 125, 240, 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 127
HpyF3I CTNAG 2 cut(s) 711, 726
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 4 cut(s) 42, 463, 538, 550
Kzo9I GATC 4 cut(s) 142, 157, 417, 554
LweI GCATC 1 cut(s) 238
MaeI CTAG 1 cut(s) 660
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 5 cut(s) 150, 199, 226, 332, 368
MalI GATC 4 cut(s) 144, 159, 419, 556
MboI GATC 4 cut(s) 142, 157, 417, 554
MboII GAAGA 1 cut(s) 698
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 3 cut(s) 654, 704, 719
MluCI AATT 4 cut(s) 87, 302, 521, 596
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 663
MnlI CCTC 5 cut(s) 182, 442, 574, 664, 729
Mph1103I ATGCAT 1 cut(s) 44
MseI TTAA 2 cut(s) 45, 174
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 412
MspR9I CCNGG 1 cut(s) 292
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 2 cut(s) 118, 127
NdeI CATATG 2 cut(s) 236, 527
NdeII GATC 4 cut(s) 142, 157, 417, 554
NlaIII CATG 4 cut(s) 42, 463, 538, 550
NlaIV GGNNCC 5 cut(s) 101, 165, 419, 489, 512
NmuCI GTSAC 2 cut(s) 226, 332
NsiI ATGCAT 1 cut(s) 44
NspI RCATGY 2 cut(s) 42, 550
OliI CACNNNNGTG 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PfoI TCCNGGA 1 cut(s) 290
PinAI ACCGGT 1 cut(s) 411
PkrI GCNGC 1 cut(s) 533
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PsiI TTATAA 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 290
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 5 cut(s) 101, 165, 419, 489, 512
PspPI GGNCC 1 cut(s) 511
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 45, 174
SatI GCNGC 1 cut(s) 532
Sau3AI GATC 4 cut(s) 142, 157, 417, 554
Sau96I GGNCC 1 cut(s) 511
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 3 cut(s) 654, 704, 719
SfaNI GCATC 1 cut(s) 238
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 4 cut(s) 87, 302, 521, 596
SsiI CCGC 3 cut(s) 275, 373, 532
SspMI CTAG 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 199, 205, 577
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 499, 567, 579
TasI AATT 4 cut(s) 87, 302, 521, 596
TauI GCSGC 1 cut(s) 534
Tru1I TTAA 2 cut(s) 45, 174
Tru9I TTAA 2 cut(s) 45, 174
TseFI GTSAC 2 cut(s) 226, 332
Tsp45I GTSAC 2 cut(s) 226, 332
TspDTI ATGAA 4 cut(s) 223, 514, 551, 553
TspGWI ACGGA 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 511
XapI RAATTY 3 cut(s) 87, 521, 596
XceI RCATGY 2 cut(s) 42, 550
XspI CTAG 1 cut(s) 660
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.