MD06G1194800.v1.1

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009773
Physical Location & Seq
Forward (+)
32944440 .. 32946628
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1194800.v1.1.491

Sequence Viewer

Length: 777 bp
ATGAACCTTGTCAGATTAAAGGGAATTCCCATTCTCCAACAGCTTCATTTAGAGGAGCAACTGCTTCGGACCTCGTCCGATAATTGGTGCATTATAAATGATGGAACTAATGATCCCGCTATAGTCATGGGTGTTTCGGGAAAACCTGCAGAGCTTCTTGAAATTGATTTGGTGTTACGAGACCAGATTCCCGTCATTAGAAGGTTTACTGGAGGTGGTACTGTTACTGTTGATCAGAATACCCTATTTGTCACTTTCATATGCAACAAGGATGCGGTTCCTGGCTTGCAACCATATCCTCGACCTATAATGTCGTGGAGTAGCCTAGTATACAGCAAAGTGTTTGAAGGACTTGCTGATTTTCAGCTTCGTGAAAACGATTATGTATTTGGTAACCGCAAGTTTGGTGGGAATGCTCAATCTATTAGTAAAAACCGGTGGATCCACCACACTTCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCATCCAAAACGGGTTCCTGAATATCGATTGGCAAGAGACCATTTAGAATTCATATGCCACATGAAAGACTACATCCCAAGATCAGTTTTTCTTGAGAAAACTGTTGAGGCAATCGGAACCCATTTCTCCGTGAGACCCGAACAGTCGGACACCATTGAAGCTACCTCGAATACGAAGTTTGTACCCTCAACTAGACTTTTGACAAGGCAGGAATTAGAGGAAGCAGCAGCTTTTGAATCTCAAGCTGAGAGAAATCTCACTCAATCATTGTCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.57

Weight (kDa)

6.39

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 11 - 208 5.3e-29 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 154
AccI GTMKAC 1 cut(s) 330
AciI CCGC 3 cut(s) 117, 275, 397
AclWI GGATC 3 cut(s) 107, 436, 449
AcsI RAATTY 2 cut(s) 24, 545
AcuI CTGAAG 1 cut(s) 515
AfaI GTAC 2 cut(s) 220, 681
AfiI CCNNNNNNNGG 2 cut(s) 84, 462
AgeI ACCGGT 1 cut(s) 435
AgsI TTSAA 4 cut(s) 161, 347, 656, 734
AjnI CCWGG 1 cut(s) 280
AloI GAACNNNNNNTCC 2 cut(s) 97, 129
AluBI AGCT 6 cut(s) 43, 154, 367, 659, 728, 743
AluI AGCT 6 cut(s) 43, 154, 367, 659, 728, 743
Alw26I GTCTC 3 cut(s) 174, 528, 625
AlwI GGATC 3 cut(s) 107, 436, 449
ApeKI GCWGC 2 cut(s) 722, 725
ApoI RAATTY 2 cut(s) 24, 545
AsiGI ACCGGT 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 69
AvaII GGWCC 1 cut(s) 69
BamHI GGATCC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 734, 737
BccI CCATC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 1 cut(s) 282
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 3 cut(s) 174, 528, 625
BfaI CTAG 2 cut(s) 326, 690
BfmI CTRYAG 2 cut(s) 120, 147
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 2 cut(s) 723, 726
BlsI GCNGC 2 cut(s) 724, 727
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 4 cut(s) 279, 443, 513, 616
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 262, 508
BpmI CTGGAG 1 cut(s) 231
BpuEI CTTGAG 2 cut(s) 611, 723
Bsa29I ATCGAT 1 cut(s) 523
BsaI GGTCTC 3 cut(s) 174, 528, 625
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 462
Bse118I RCCGGY 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 523
BseGI GGATG 3 cut(s) 277, 499, 570
BseLI CCNNNNNNNGG 2 cut(s) 84, 462
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 734, 737
BshTI ACCGGT 1 cut(s) 435
BshVI ATCGAT 1 cut(s) 523
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 2 cut(s) 84, 462
BsmAI GTCTC 3 cut(s) 174, 528, 625
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 418
Bso31I GGTCTC 3 cut(s) 174, 528, 625
Bsp143I GATC 4 cut(s) 112, 232, 441, 578
BspACI CCGC 3 cut(s) 117, 275, 397
BspCNI CTCAG 1 cut(s) 736
BspDI ATCGAT 1 cut(s) 523
BspLI GGNNCC 4 cut(s) 279, 443, 513, 616
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 3 cut(s) 107, 436, 449
BspTNI GGTCTC 3 cut(s) 174, 528, 625
BsrFI RCCGGY 1 cut(s) 435
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 435
BssMI GATC 4 cut(s) 112, 232, 441, 578
BssNAI GTATAC 1 cut(s) 331
Bst1107I GTATAC 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 4 cut(s) 223, 229, 601, 642
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 1 cut(s) 744
BstEII GGTNACC 1 cut(s) 392
BstF5I GGATG 3 cut(s) 277, 499, 570
BstKTI GATC 4 cut(s) 115, 235, 444, 581
BstMAI GTCTC 3 cut(s) 174, 528, 625
BstMBI GATC 4 cut(s) 112, 232, 441, 578
BstNI CCWGG 1 cut(s) 282
BstPI GGTNACC 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 2 cut(s) 120, 147
BstV1I GCAGC 2 cut(s) 734, 737
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
BstZ17I GTATAC 1 cut(s) 331
Bsu15I ATCGAT 1 cut(s) 523
BsuTUI ATCGAT 1 cut(s) 523
BtsCI GGATG 3 cut(s) 277, 499, 570
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 69
ClaI ATCGAT 1 cut(s) 523
Csp6I GTAC 2 cut(s) 219, 680
CspAI ACCGGT 1 cut(s) 435
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 3 cut(s) 127, 484, 559
CviJI RGCY 8 cut(s) 43, 154, 285, 324, 367, 659, 728, 743
CviKI_1 RGCY 8 cut(s) 43, 154, 285, 324, 367, 659, 728, 743
CviQI GTAC 2 cut(s) 219, 680
DdeI CTNAG 1 cut(s) 744
DpnI GATC 4 cut(s) 114, 234, 443, 580
DpnII GATC 4 cut(s) 112, 232, 441, 578
Eco31I GGTCTC 3 cut(s) 174, 528, 625
Eco47I GGWCC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 515
Eco91I GGTNACC 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 24, 545
EcoRII CCWGG 1 cut(s) 280
FaeI CATG 3 cut(s) 130, 487, 562
FaqI GGGAC 1 cut(s) 479
FatI CATG 3 cut(s) 126, 483, 558
FauI CCCGC 1 cut(s) 124
FauNDI CATATG 2 cut(s) 260, 551
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 2 cut(s) 723, 726
FokI GGATG 3 cut(s) 284, 486, 557
Fsp4HI GCNGC 2 cut(s) 723, 726
FspBI CTAG 2 cut(s) 326, 690
GluI GCNGC 2 cut(s) 723, 726
GsuI CTGGAG 1 cut(s) 231
HapII CCGG 1 cut(s) 436
Hin1II CATG 3 cut(s) 130, 487, 562
HinfI GANTC 2 cut(s) 187, 734
HpaII CCGG 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 207, 331
Hpy188I TCNGA 6 cut(s) 14, 69, 79, 237, 614, 646
Hpy188III TCNNGA 5 cut(s) 138, 158, 371, 515, 590
Hpy8I GTNNAC 2 cut(s) 207, 331
HpyAV CCTTC 2 cut(s) 195, 341
HpyCH4III ACNGT 4 cut(s) 223, 229, 601, 642
HpyCH4V TGCA 4 cut(s) 90, 149, 264, 289
HpyF3I CTNAG 1 cut(s) 744
Hsp92II CATG 3 cut(s) 130, 487, 562
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 4 cut(s) 112, 232, 441, 578
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 9 cut(s) 159, 195, 197, 267, 294, 449, 506, 528, 692
Lsp1109I GCAGC 2 cut(s) 734, 737
LweI GCATC 2 cut(s) 262, 508
MaeI CTAG 2 cut(s) 326, 690
MaeIII GTNAC 4 cut(s) 174, 223, 250, 392
MalI GATC 4 cut(s) 114, 234, 443, 580
MboI GATC 4 cut(s) 112, 232, 441, 578
MflI RGATCY 1 cut(s) 441
MluCI AATT 5 cut(s) 24, 82, 162, 545, 710
MmeI TCCRAC 2 cut(s) 61, 624
MnlI CCTC 9 cut(s) 46, 82, 206, 309, 466, 598, 673, 694, 709
MseI TTAA 1 cut(s) 17
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 282
NdeI CATATG 2 cut(s) 260, 551
NdeII GATC 4 cut(s) 112, 232, 441, 578
NlaIII CATG 3 cut(s) 130, 487, 562
NlaIV GGNNCC 4 cut(s) 279, 443, 513, 616
NmuCI GTSAC 1 cut(s) 250
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 2 cut(s) 187, 734
PflFI GACNNNGTC 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 2 cut(s) 724, 727
PsiI TTATAA 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 280
PspEI GGTNACC 1 cut(s) 392
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 4 cut(s) 279, 443, 513, 616
PspPI GGNCC 1 cut(s) 69
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 441
PsyI GACNNNGTC 1 cut(s) 73
RsaI GTAC 2 cut(s) 220, 681
RsaNI GTAC 2 cut(s) 219, 680
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 2 cut(s) 723, 726
Sau3AI GATC 4 cut(s) 112, 232, 441, 578
Sau96I GGNCC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 282
SfaNI GCATC 2 cut(s) 262, 508
SfcI CTRYAG 2 cut(s) 120, 147
SinI GGWCC 1 cut(s) 69
SmlI CTYRAG 2 cut(s) 590, 738
SmoI CTYRAG 2 cut(s) 590, 738
Sse9I AATT 5 cut(s) 24, 82, 162, 545, 710
SsiI CCGC 3 cut(s) 117, 275, 397
SspMI CTAG 2 cut(s) 326, 690
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 4 cut(s) 223, 229, 601, 642
TaqI TCGA 3 cut(s) 301, 523, 665
TasI AATT 5 cut(s) 24, 82, 162, 545, 710
TfiI GAWTC 2 cut(s) 187, 734
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 722, 725
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 17, 35, 247, 538, 575
TspGWI ACGGA 1 cut(s) 616
Tth111I GACNNNGTC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 69
XapI RAATTY 2 cut(s) 24, 545
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 2 cut(s) 326, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.