FvH4_5g14790

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009773
Physical Location & Seq
Reverse (-)
8397786 .. 8400336
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14790.t1

Sequence Viewer

Length: 783 bp
ATGGCTATACCTCAAAGAAATGTTGGGCTTCCATTGATGAACCTTGTCAGATTAAAAGGAACTCCCATTCTGCAGCAGCTTCATTTAGAGGAGCGGCTGCTGCGAACCTCGTCTGATAATTGGTGCATTATAAATGATGGCACAAATGATCGTAATATTGTCATGGGTATTTCAGGAAAACATGCAGAGCTTCTAGACATTGATCTGGTGTTACGAGATCAGGTTCCTGTCATTAAAAGGTATACTGGTGGAGGCACAGTTGCAGTTGATCATGGCACCATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTGGCTTGAAACTATATCCGCGACCTATAATGTCTTGGAGTAGCCAATTGTACAACAGAGTGTTCAAAAGATTTGGTGATTTTTACCTTCTTGAAAATGATTATGTATTTGGTAATCGCAAGTTTGGTGGGAATGCTCAATGTATTACGAAAGATCGGTGGATCCACCACACATCCTTCCTATGGGACTATGAGGAGAGAAACATGGCATACCTGAAGCATCCAAAACGGGCTAGGGATCATTTGGAATTTGTATGCCGCATGAAGGACTACGTGCCAAGATCAGTTTTCATCCAGAAAACTGTCGAGGCAATCAGTGCTGAATTCTCAGTAGAATCTGAACATACAGACCCATTTGAAACCAACTCAAAGTTTGTGCCCTCAACAAGACTATTGACAAGGCAGGAGTTGGAAGAAGCAGCTGTAGCCTCTCAATCCGCGAGCTCTCTCTCTCAGTCGTCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.84

Weight (kDa)

8.31

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 23 - 214 1.5e-28 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 94
AccI GTMKAC 1 cut(s) 242
AccII CGCG 2 cut(s) 337, 755
AciI CCGC 4 cut(s) 94, 335, 574, 753
AclWI GGATC 3 cut(s) 472, 485, 561
AcsI RAATTY 2 cut(s) 563, 638
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 2 cut(s) 498, 580
AgsI TTSAA 4 cut(s) 325, 382, 410, 674
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 4 cut(s) 79, 190, 737, 759
AluI AGCT 4 cut(s) 79, 190, 737, 759
Alw21I GWGCWC 1 cut(s) 761
AlwI GGATC 3 cut(s) 472, 485, 561
ApeKI GCWGC 5 cut(s) 73, 76, 97, 100, 734
ApoI RAATTY 2 cut(s) 563, 638
AsuHPI GGTGA 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 696
BamHI GGATCC 1 cut(s) 477
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 761
BbvI GCAGC 5 cut(s) 84, 85, 87, 88, 746
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 1 cut(s) 268
BfaI CTAG 2 cut(s) 194, 549
BfmI CTRYAG 2 cut(s) 71, 738
BisI GCNGC 7 cut(s) 74, 77, 95, 98, 101, 574, 735
BlsI GCNGC 7 cut(s) 75, 78, 96, 99, 102, 575, 736
Bme1390I CCNGG 1 cut(s) 318
BmiI GGNNCC 3 cut(s) 225, 277, 479
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 2 cut(s) 298, 544
BsaAI YACGTR 1 cut(s) 589
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 498, 580
Bse1I ACTGG 1 cut(s) 250
BseBI CCWGG 1 cut(s) 318
BseGI GGATG 4 cut(s) 313, 488, 535, 606
BseLI CCNNNNNNNGG 2 cut(s) 498, 580
BseMII CTCAG 2 cut(s) 657, 782
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 104, 524
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 5 cut(s) 84, 85, 87, 88, 746
Bsh1236I CGCG 2 cut(s) 337, 755
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 761
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 498, 580
BsmFI GGGAC 1 cut(s) 515
BsmI GAATGC 1 cut(s) 454
Bsp1286I GDGCHC 2 cut(s) 696, 761
Bsp1407I TGTACA 1 cut(s) 366
Bsp143I GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
BspACI CCGC 4 cut(s) 94, 335, 574, 753
BspCNI CTCAG 2 cut(s) 656, 781
BspFNI CGCG 2 cut(s) 337, 755
BspLI GGNNCC 3 cut(s) 225, 277, 479
BspMAI CTGCAG 1 cut(s) 75
BspPI GGATC 3 cut(s) 472, 485, 561
BspT107I GGYRCC 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 1 cut(s) 250
BssMI GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
BssNAI GTATAC 1 cut(s) 243
Bst1107I GTATAC 1 cut(s) 243
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 259, 619
BstAPI GCANNNNNTGC 1 cut(s) 632
BstAUI TGTACA 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 757
BstDEI CTNAG 2 cut(s) 643, 768
BstF5I GGATG 4 cut(s) 313, 488, 535, 606
BstFNI CGCG 2 cut(s) 337, 755
BstKTI GATC 8 cut(s) 151, 205, 220, 271, 472, 480, 556, 599
BstMBI GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
BstMWI GCNNNNNNNGC 3 cut(s) 100, 632, 740
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 2 cut(s) 71, 738
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 2 cut(s) 337, 755
BstV1I GCAGC 5 cut(s) 84, 85, 87, 88, 746
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BstZ17I GTATAC 1 cut(s) 243
BtsCI GGATG 4 cut(s) 313, 488, 535, 606
BtsIMutI CAGTG 1 cut(s) 637
Cac8I GCNNGC 1 cut(s) 757
Csp6I GTAC 1 cut(s) 367
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 5 cut(s) 163, 182, 272, 520, 577
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 643, 768
DpnI GATC 8 cut(s) 150, 204, 219, 270, 471, 479, 555, 598
DpnII GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
Ecl136II GAGCTC 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 551
EcoICRI GAGCTC 1 cut(s) 759
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 761
FaeI CATG 5 cut(s) 166, 185, 275, 523, 580
FaqI GGGAC 1 cut(s) 515
FatI CATG 5 cut(s) 162, 181, 271, 519, 576
FauNDI CATATG 1 cut(s) 296
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 7 cut(s) 74, 77, 95, 98, 101, 574, 735
FokI GGATG 4 cut(s) 320, 475, 522, 593
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 7 cut(s) 74, 77, 95, 98, 101, 574, 735
FspBI CTAG 2 cut(s) 194, 549
GluI GCNGC 7 cut(s) 74, 77, 95, 98, 101, 574, 735
Hin1II CATG 5 cut(s) 166, 185, 275, 523, 580
HinfI GANTC 1 cut(s) 650
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 3 cut(s) 50, 115, 655
Hpy188III TCNNGA 4 cut(s) 174, 194, 407, 610
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 413, 502, 574
HpyCH4III ACNGT 2 cut(s) 259, 619
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 5 cut(s) 73, 126, 185, 263, 300
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 632, 740
HpyF3I CTNAG 2 cut(s) 643, 768
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 5 cut(s) 166, 185, 275, 523, 580
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 5 cut(s) 84, 85, 87, 88, 746
LweI GCATC 2 cut(s) 298, 544
MaeI CTAG 2 cut(s) 194, 549
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 2 cut(s) 210, 286
MalI GATC 8 cut(s) 150, 204, 219, 270, 471, 479, 555, 598
MbiI CCGCTC 1 cut(s) 94
MboI GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
MboII GAAGA 1 cut(s) 740
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 2 cut(s) 696, 761
MluCI AATT 4 cut(s) 118, 362, 563, 638
MmeI TCCRAC 1 cut(s) 705
MnlI CCTC 8 cut(s) 21, 82, 118, 245, 502, 616, 706, 754
MseI TTAA 2 cut(s) 53, 234
MspA1I CMGCKG 1 cut(s) 737
MspR9I CCNGG 1 cut(s) 318
MunI CAATTG 1 cut(s) 362
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 2 cut(s) 337, 755
MwoI GCNNNNNNNGC 3 cut(s) 100, 632, 740
NdeI CATATG 1 cut(s) 296
NdeII GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
NlaIII CATG 5 cut(s) 166, 185, 275, 523, 580
NlaIV GGNNCC 3 cut(s) 225, 277, 479
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 185
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 650
PkrI GCNGC 7 cut(s) 75, 78, 96, 99, 102, 575, 736
Ppu21I YACGTR 1 cut(s) 589
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 225, 277, 479
PstI CTGCAG 1 cut(s) 75
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SacI GAGCTC 1 cut(s) 761
SaqAI TTAA 2 cut(s) 53, 234
SatI GCNGC 7 cut(s) 74, 77, 95, 98, 101, 574, 735
Sau3AI GATC 8 cut(s) 148, 202, 217, 268, 469, 477, 553, 596
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 2 cut(s) 696, 761
SfaNI GCATC 2 cut(s) 298, 544
SfcI CTRYAG 2 cut(s) 71, 738
Sse9I AATT 4 cut(s) 118, 362, 563, 638
SsiI CCGC 4 cut(s) 94, 335, 574, 753
SspI AATATT 1 cut(s) 157
SspMI CTAG 2 cut(s) 194, 549
SstI GAGCTC 1 cut(s) 761
StyD4I CCNGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 259, 619
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 621
TasI AATT 4 cut(s) 118, 362, 563, 638
TatI WGTACW 1 cut(s) 366
TauI GCSGC 2 cut(s) 97, 576
TfiI GAWTC 1 cut(s) 650
Tru1I TTAA 2 cut(s) 53, 234
Tru9I TTAA 2 cut(s) 53, 234
TscAI CASTG 1 cut(s) 637
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 5 cut(s) 73, 76, 97, 100, 734
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 5 cut(s) 53, 71, 283, 593, 595
TspRI CASTG 1 cut(s) 637
XapI RAATTY 2 cut(s) 563, 638
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 1 cut(s) 185
XmiI GTMKAC 1 cut(s) 242
XspI CTAG 2 cut(s) 194, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.