Rh7BG043700

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009773
Physical Location & Seq
Forward (+)
2759798 .. 2763205
3408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG043700.1

Sequence Viewer

Length: 1005 bp
ATGTTCTTGCCAGGCCGGCCTTTCCCTTCCTGGGCTGGGCCGGTACCTGAGGCTACTGGGCTTACGGGCCCAAATGTCGCCCTAACATTCTTCACCAAAAGTCCAAAAGGCACAAGCTTCTCTTTCAGCGACTTCGTGACACAGAGTCTCCGGCTTTATCTTCCTCAACCTCAGATTTCAGTCGACAGCGGAAACAGGATGACTATACCTCAAATAAGAAACGTTGGGCTTCCATTGATGAACCTTATCAGATTAAAAGGAACTCCCATCCTGCAGCAGCTTCATTTAGAGGAGCAACTGCTTCGAACGTCATCTGATAATTGGTGCATTATAAATGATGGAACCAATAGTCCTAATATTGTCATGGGTATTTCAGGAAAGCCTGCAGAGCTTCTTGACGTTGATCTGGTGTTACGAGATCGGGTTCCTGTCATTAAAAGGTTTACTGGAGGTGGCACGGTTACAGTTGATAATGGCACGATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTGGCTTGCAACTATATCCGCGACCTATAATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAGGATTTGGTGACTTCCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAATCTATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGTTCCTGAATATCGATTGGCAAGGGACCATTTGGAATTCATATGCCGCATGAAGGACTACATGCCAAGATCAGTTTTCATCGACAAAACTGTCGAGGCAATCAGTACCGAATTTTCAGTCAAATCTGAACAAACAGACCCATTCAAAACCAACTCAAACTTTGTGCCCTCAACTAGACTATTGACAAGGCAAGAGTTGGAAGAAGTAGCTTTGGCCTCTCAACCTGAGAGCCCTCTCTCTGAGTCCTTGTCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

334

Amino Acids

37.82

Weight (kDa)

8.92

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 90 - 287 6.4e-29 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AasI GACNNNNNNGTC 1 cut(s) 836
Acc65I GGTACC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 538
AciI CCGC 4 cut(s) 189, 536, 634, 793
AclI AACGTT 1 cut(s) 222
AclWI GGATC 2 cut(s) 673, 686
AcsI RAATTY 2 cut(s) 782, 857
AcuI CTGAAG 1 cut(s) 752
AfaI GTAC 2 cut(s) 45, 853
AfiI CCNNNNNNNGG 4 cut(s) 31, 36, 699, 799
AgeI ACCGGT 1 cut(s) 672
AgsI TTSAA 1 cut(s) 892
AhdI GACNNNNNGTC 1 cut(s) 144
AjnI CCWGG 4 cut(s) 10, 29, 517, 551
AleI CACNNNNGTG 1 cut(s) 606
AloI GAACNNNNNNTCC 2 cut(s) 334, 366
AluBI AGCT 5 cut(s) 117, 280, 391, 736, 956
AluI AGCT 5 cut(s) 117, 280, 391, 736, 956
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 673, 686
AoxI GGCC 5 cut(s) 13, 17, 38, 67, 960
ApaI GGGCCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 274, 277
ApoI RAATTY 2 cut(s) 782, 857
ArsI GACNNNNNNTTYG 2 cut(s) 849, 881
AsiGI ACCGGT 1 cut(s) 672
Asp718I GGTACC 1 cut(s) 43
AspS9I GGNCC 4 cut(s) 38, 67, 68, 772
AsuHPI GGTGA 2 cut(s) 85, 605
AsuII TTCGAA 1 cut(s) 304
AvaII GGWCC 1 cut(s) 772
AxyI CCTNAGG 1 cut(s) 48
BaeGI GKGCMC 2 cut(s) 71, 915
BamHI GGATCC 1 cut(s) 678
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 2 cut(s) 71, 980
BbvI GCAGC 2 cut(s) 286, 289
BccI CCATC 2 cut(s) 275, 332
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 4 cut(s) 12, 31, 519, 553
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 921
BfmI CTRYAG 2 cut(s) 272, 384
BglI GCCNNNNNGGC 1 cut(s) 16
BisI GCNGC 3 cut(s) 275, 278, 793
BlsI GCNGC 3 cut(s) 276, 279, 794
Bme1390I CCNGG 4 cut(s) 12, 31, 519, 553
Bme18I GGWCC 1 cut(s) 772
BmeRI GACNNNNNGTC 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 38, 67, 68, 772
BmiI GGNNCC 7 cut(s) 45, 69, 343, 426, 680, 750, 773
BmrFI CCNGG 4 cut(s) 12, 31, 519, 553
BmrI ACTGGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 499
BmuI ACTGGG 1 cut(s) 66
BpmI CTGGAG 2 cut(s) 468, 574
Bpu14I TTCGAA 1 cut(s) 304
Bsa29I ATCGAT 1 cut(s) 760
BsaJI CCNNGG 2 cut(s) 30, 583
BsaWI WCCGGW 1 cut(s) 672
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 36, 699, 799
Bse118I RCCGGY 3 cut(s) 15, 40, 672
Bse1I ACTGG 2 cut(s) 61, 451
Bse21I CCTNAGG 1 cut(s) 48
BseBI CCWGG 4 cut(s) 12, 31, 519, 553
BseCI ATCGAT 1 cut(s) 760
BseDI CCNNGG 2 cut(s) 30, 583
BseGI GGATG 4 cut(s) 204, 267, 514, 689
BseLI CCNNNNNNNGG 4 cut(s) 31, 36, 699, 799
BseMII CTCAG 4 cut(s) 39, 185, 963, 978
BseNI ACTGG 2 cut(s) 61, 451
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 2 cut(s) 71, 915
BseXI GCAGC 2 cut(s) 286, 289
BseYI CCCAGC 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 538
BshFI GGCC 5 cut(s) 15, 19, 40, 69, 962
BshNI GGYRCC 1 cut(s) 43
BshTI ACCGGT 1 cut(s) 672
BshVI ATCGAT 1 cut(s) 760
BsiSI CCGG 4 cut(s) 16, 41, 151, 673
BslFI GGGAC 2 cut(s) 716, 785
BslI CCNNNNNNNGG 4 cut(s) 31, 36, 699, 799
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 716, 785
BsnI GGCC 5 cut(s) 15, 19, 40, 69, 962
Bsp119I TTCGAA 1 cut(s) 304
Bsp120I GGGCCC 1 cut(s) 67
Bsp1286I GDGCHC 3 cut(s) 71, 915, 980
Bsp143I GATC 4 cut(s) 403, 418, 678, 815
BspACI CCGC 4 cut(s) 189, 536, 634, 793
BspANI GGCC 5 cut(s) 15, 19, 40, 69, 962
BspCNI CTCAG 4 cut(s) 40, 184, 964, 979
BspDI ATCGAT 1 cut(s) 760
BspFNI CGCG 1 cut(s) 538
BspLI GGNNCC 7 cut(s) 45, 69, 343, 426, 680, 750, 773
BspMAI CTGCAG 2 cut(s) 276, 388
BspPI GGATC 2 cut(s) 673, 686
BspT104I TTCGAA 1 cut(s) 304
BspT107I GGYRCC 1 cut(s) 43
BsrFI RCCGGY 3 cut(s) 15, 40, 672
BsrI ACTGG 2 cut(s) 61, 451
BssAI RCCGGY 3 cut(s) 15, 40, 672
BssECI CCNNGG 2 cut(s) 30, 583
BssMI GATC 4 cut(s) 403, 418, 678, 815
BssT1I CCWWGG 1 cut(s) 583
Bst2UI CCWGG 4 cut(s) 12, 31, 519, 553
Bst4CI ACNGT 3 cut(s) 460, 466, 838
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 17, 384, 524
BstDEI CTNAG 4 cut(s) 48, 171, 972, 987
BstEII GGTNACC 1 cut(s) 629
BstF5I GGATG 4 cut(s) 204, 267, 514, 689
BstFNI CGCG 1 cut(s) 538
BstKTI GATC 4 cut(s) 406, 421, 681, 818
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 4 cut(s) 403, 418, 678, 815
BstMWI GCNNNNNNNGC 2 cut(s) 16, 388
BstNI CCWGG 4 cut(s) 12, 31, 519, 553
BstNSI RCATGY 1 cut(s) 811
BstPI GGTNACC 1 cut(s) 629
BstSCI CCNGG 4 cut(s) 10, 29, 517, 551
BstSFI CTRYAG 2 cut(s) 272, 384
BstSLI GKGCMC 2 cut(s) 71, 915
BstUI CGCG 1 cut(s) 538
BstV1I GCAGC 2 cut(s) 286, 289
BstX2I RGATCY 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 590
BstYI RGATCY 1 cut(s) 678
Bsu15I ATCGAT 1 cut(s) 760
Bsu36I CCTNAGG 1 cut(s) 48
BsuRI GGCC 5 cut(s) 15, 19, 40, 69, 962
BsuTUI ATCGAT 1 cut(s) 760
BtsCI GGATG 4 cut(s) 204, 267, 514, 689
Cac8I GCNNGC 3 cut(s) 17, 384, 524
Cfr10I RCCGGY 3 cut(s) 15, 40, 672
Cfr13I GGNCC 4 cut(s) 38, 67, 68, 772
ClaI ATCGAT 1 cut(s) 760
Csp6I GTAC 2 cut(s) 44, 852
CspAI ACCGGT 1 cut(s) 672
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 4 cut(s) 364, 721, 796, 808
CviQI GTAC 2 cut(s) 44, 852
DdeI CTNAG 4 cut(s) 48, 171, 972, 987
DpnI GATC 4 cut(s) 405, 420, 680, 817
DpnII GATC 4 cut(s) 403, 418, 678, 815
DrdI GACNNNNNNGTC 1 cut(s) 836
DriI GACNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 836
Eam1105I GACNNNNNGTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 583
Eco24I GRGCYC 2 cut(s) 71, 980
Eco47I GGWCC 1 cut(s) 772
Eco57I CTGAAG 1 cut(s) 752
Eco81I CCTNAGG 1 cut(s) 48
Eco91I GGTNACC 1 cut(s) 629
EcoO65I GGTNACC 1 cut(s) 629
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 4 cut(s) 10, 29, 517, 551
EcoT14I CCWWGG 1 cut(s) 583
EcoT38I GRGCYC 2 cut(s) 71, 980
ErhI CCWWGG 1 cut(s) 583
FaeI CATG 4 cut(s) 367, 724, 799, 811
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 2 cut(s) 716, 785
FatI CATG 4 cut(s) 363, 720, 795, 807
FauNDI CATATG 2 cut(s) 497, 788
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 275, 278, 793
FokI GGATG 4 cut(s) 211, 254, 521, 676
FriOI GRGCYC 2 cut(s) 71, 980
FseI GGCCGGCC 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 275, 278, 793
FspBI CTAG 1 cut(s) 921
GluI GCNGC 3 cut(s) 275, 278, 793
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 468, 574
HaeIII GGCC 5 cut(s) 15, 19, 40, 69, 962
HapII CCGG 4 cut(s) 16, 41, 151, 673
Hin1II CATG 4 cut(s) 367, 724, 799, 811
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 2 cut(s) 115, 734
HinfI GANTC 2 cut(s) 145, 989
HpaII CCGG 4 cut(s) 16, 41, 151, 673
HphI GGTGA 2 cut(s) 85, 605
Hpy166II GTNNAC 2 cut(s) 184, 444
Hpy188I TCNGA 5 cut(s) 174, 251, 316, 874, 988
Hpy188III TCNNGA 5 cut(s) 136, 375, 395, 608, 752
Hpy8I GTNNAC 2 cut(s) 184, 444
HpyAV CCTTC 3 cut(s) 36, 614, 793
HpyCH4III ACNGT 3 cut(s) 460, 466, 838
HpyCH4IV ACGT 3 cut(s) 222, 308, 399
HpyCH4V TGCA 5 cut(s) 274, 327, 386, 501, 526
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 388
HpyF3I CTNAG 4 cut(s) 48, 171, 972, 987
HpySE526I ACGT 3 cut(s) 222, 308, 399
Hsp92II CATG 4 cut(s) 367, 724, 799, 811
KpnI GGTACC 1 cut(s) 47
KroI GCCGGC 1 cut(s) 15
KroNI GCCGGC 1 cut(s) 17
Kzo9I GATC 4 cut(s) 403, 418, 678, 815
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 2 cut(s) 286, 289
LweI GCATC 1 cut(s) 499
MaeI CTAG 1 cut(s) 921
MaeII ACGT 3 cut(s) 222, 308, 399
MaeIII GTNAC 6 cut(s) 136, 411, 460, 487, 593, 629
MalI GATC 4 cut(s) 405, 420, 680, 817
MboI GATC 4 cut(s) 403, 418, 678, 815
MboII GAAGA 3 cut(s) 82, 152, 959
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 3 cut(s) 71, 915, 980
MluCI AATT 4 cut(s) 319, 563, 782, 857
MlyI GAGTC 2 cut(s) 154, 998
MmeI TCCRAC 1 cut(s) 924
MroNI GCCGGC 1 cut(s) 15
MseI TTAA 2 cut(s) 254, 435
MslI CAYNNNNRTG 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 4 cut(s) 16, 41, 151, 673
MspR9I CCNGG 4 cut(s) 12, 31, 519, 553
MvaI CCWGG 4 cut(s) 12, 31, 519, 553
MvnI CGCG 1 cut(s) 538
MwoI GCNNNNNNNGC 2 cut(s) 16, 388
NaeI GCCGGC 1 cut(s) 17
NdeI CATATG 2 cut(s) 497, 788
NdeII GATC 4 cut(s) 403, 418, 678, 815
NgoMIV GCCGGC 1 cut(s) 15
NlaIII CATG 4 cut(s) 367, 724, 799, 811
NlaIV GGNNCC 7 cut(s) 45, 69, 343, 426, 680, 750, 773
NmuCI GTSAC 3 cut(s) 136, 487, 593
NspI RCATGY 1 cut(s) 811
NspV TTCGAA 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 606
PdiI GCCGGC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 551
PinAI ACCGGT 1 cut(s) 672
PkrI GCNGC 3 cut(s) 276, 279, 794
PleI GAGTC 2 cut(s) 153, 997
PpsI GAGTC 2 cut(s) 153, 997
PsiI TTATAA 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 222
Psp6I CCWGG 4 cut(s) 10, 29, 517, 551
PspEI GGTNACC 1 cut(s) 629
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 4 cut(s) 10, 29, 517, 551
PspN4I GGNNCC 7 cut(s) 45, 69, 343, 426, 680, 750, 773
PspOMI GGGCCC 1 cut(s) 67
PspPI GGNCC 4 cut(s) 38, 67, 68, 772
PsrI GAACNNNNNNTAC 2 cut(s) 710, 742
PstI CTGCAG 2 cut(s) 276, 388
PsuI RGATCY 1 cut(s) 678
RigI GGCCGGCC 1 cut(s) 19
RsaI GTAC 2 cut(s) 45, 853
RsaNI GTAC 2 cut(s) 44, 852
RseI CAYNNNNRTG 1 cut(s) 606
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 254, 435
SatI GCNGC 3 cut(s) 275, 278, 793
Sau3AI GATC 4 cut(s) 403, 418, 678, 815
Sau96I GGNCC 4 cut(s) 38, 67, 68, 772
SchI GAGTC 2 cut(s) 154, 998
ScrFI CCNGG 4 cut(s) 12, 31, 519, 553
SduI GDGCHC 3 cut(s) 71, 915, 980
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 2 cut(s) 272, 384
SfuI TTCGAA 1 cut(s) 304
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 1 cut(s) 606
Sse9I AATT 4 cut(s) 319, 563, 782, 857
SsiI CCGC 4 cut(s) 189, 536, 634, 793
SspI AATATT 1 cut(s) 358
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 4 cut(s) 10, 29, 517, 551
StyI CCWWGG 1 cut(s) 583
TaaI ACNGT 3 cut(s) 460, 466, 838
TaiI ACGT 3 cut(s) 225, 311, 402
TaqI TCGA 5 cut(s) 183, 304, 760, 828, 840
TasI AATT 4 cut(s) 319, 563, 782, 857
TauI GCSGC 1 cut(s) 795
Tru1I TTAA 2 cut(s) 254, 435
Tru9I TTAA 2 cut(s) 254, 435
TseFI GTSAC 3 cut(s) 136, 487, 593
TseI GCWGC 2 cut(s) 274, 277
Tsp45I GTSAC 3 cut(s) 136, 487, 593
TspDTI ATGAA 6 cut(s) 254, 272, 484, 775, 812, 814
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 2 cut(s) 782, 857
XceI RCATGY 1 cut(s) 811
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.