Rroxscaffold_3G00271870

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009773
Physical Location & Seq
Reverse (-)
64192930 .. 64195317
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271870.1

Sequence Viewer

Length: 459 bp
ATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAGGATTTGGTGACTTTCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAGTCTATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGTTCCTGAATATCGATTGGCAAGGGACCATTTGGAATTCATATGCTGCATGAAGGACTACATGCCGAGATCAGTTTTCATCGACAAAACTGTCAAGGCAATCAGTACCGAATTTTCAGTCAAATCTGAACAAACAGACCCATTCAAAACCAACTCAAACTTTGTGCCCTCAACTAGACTATTGACAAGGCAAGAGTTGGAAGAAGTAGCTTTGGCCTCTCAACCTGAGAGCCCTCTCTCGGAGTCCCTGTCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.84

Weight (kDa)

8.55

Isoelectric Point (pI)

59.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 22 - 105 3.6e-10 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 290
AciI CCGC 1 cut(s) 88
AclWI GGATC 2 cut(s) 127, 140
AcsI RAATTY 2 cut(s) 236, 311
AcuI CTGAAG 1 cut(s) 206
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 2 cut(s) 153, 439
AgeI ACCGGT 1 cut(s) 126
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 1 cut(s) 5
AleI CACNNNNGTG 1 cut(s) 60
AluBI AGCT 2 cut(s) 190, 410
AluI AGCT 2 cut(s) 190, 410
AlwI GGATC 2 cut(s) 127, 140
AoxI GGCC 1 cut(s) 414
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 2 cut(s) 236, 311
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AsiGI ACCGGT 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 47, 59
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 369
BamHI GGATCC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 434
BbvI GCAGC 1 cut(s) 233
BciT130I CCWGG 1 cut(s) 7
BfaI CTAG 1 cut(s) 375
BisI GCNGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 3 cut(s) 134, 204, 227
BmrFI CCNGG 1 cut(s) 7
BpmI CTGGAG 1 cut(s) 28
Bsa29I ATCGAT 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 37
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 1 cut(s) 434
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 439
Bse118I RCCGGY 1 cut(s) 126
BseBI CCWGG 1 cut(s) 7
BseCI ATCGAT 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 2 cut(s) 153, 439
BseMII CTCAG 2 cut(s) 122, 417
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 233
BshFI GGCC 1 cut(s) 416
BshTI ACCGGT 1 cut(s) 126
BshVI ATCGAT 1 cut(s) 214
BsiSI CCGG 1 cut(s) 127
BslFI GGGAC 3 cut(s) 170, 239, 430
BslI CCNNNNNNNGG 2 cut(s) 153, 439
BsmFI GGGAC 3 cut(s) 170, 239, 430
BsnI GGCC 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 369, 434
Bsp143I GATC 2 cut(s) 132, 269
BspACI CCGC 1 cut(s) 88
BspANI GGCC 1 cut(s) 416
BspCNI CTCAG 2 cut(s) 121, 418
BspDI ATCGAT 1 cut(s) 214
BspLI GGNNCC 3 cut(s) 134, 204, 227
BspPI GGATC 2 cut(s) 127, 140
BsrFI RCCGGY 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 2 cut(s) 132, 269
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 292
BstDEI CTNAG 2 cut(s) 108, 426
BstEII GGTNACC 1 cut(s) 83
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 2 cut(s) 135, 272
BstMBI GATC 2 cut(s) 132, 269
BstNI CCWGG 1 cut(s) 7
BstNSI RCATGY 1 cut(s) 265
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 5
BstSLI GKGCMC 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 1 cut(s) 132
Bsu15I ATCGAT 1 cut(s) 214
BsuRI GGCC 1 cut(s) 416
BsuTUI ATCGAT 1 cut(s) 214
BtsCI GGATG 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 214
Csp6I GTAC 1 cut(s) 306
CspAI ACCGGT 1 cut(s) 126
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 3 cut(s) 175, 250, 262
CviJI RGCY 5 cut(s) 15, 190, 410, 416, 432
CviKI_1 RGCY 5 cut(s) 15, 190, 410, 416, 432
CviQI GTAC 1 cut(s) 306
DdeI CTNAG 2 cut(s) 108, 426
DpnI GATC 2 cut(s) 134, 271
DpnII GATC 2 cut(s) 132, 269
DrdI GACNNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 206
Eco91I GGTNACC 1 cut(s) 83
EcoO65I GGTNACC 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 3 cut(s) 178, 253, 265
FaqI GGGAC 3 cut(s) 170, 239, 430
FatI CATG 3 cut(s) 174, 249, 261
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 1 cut(s) 247
FokI GGATG 1 cut(s) 130
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 1 cut(s) 247
FspBI CTAG 1 cut(s) 375
GluI GCNGC 1 cut(s) 247
GsuI CTGGAG 1 cut(s) 28
HaeIII GGCC 1 cut(s) 416
HapII CCGG 1 cut(s) 127
Hin1II CATG 3 cut(s) 178, 253, 265
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 443
HpaII CCGG 1 cut(s) 127
HphI GGTGA 2 cut(s) 47, 59
Hpy188I TCNGA 2 cut(s) 328, 442
Hpy188III TCNNGA 2 cut(s) 62, 206
HpyAV CCTTC 2 cut(s) 68, 247
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4V TGCA 1 cut(s) 249
HpyF3I CTNAG 2 cut(s) 108, 426
Hsp92II CATG 3 cut(s) 178, 253, 265
Kzo9I GATC 2 cut(s) 132, 269
LpnPI CCDG 5 cut(s) 19, 140, 197, 219, 438
Lsp1109I GCAGC 1 cut(s) 233
MaeI CTAG 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 47, 83
MalI GATC 2 cut(s) 134, 271
MboI GATC 2 cut(s) 132, 269
MboII GAAGA 1 cut(s) 413
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 369, 434
MluCI AATT 3 cut(s) 17, 236, 311
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 4 cut(s) 157, 379, 427, 444
MslI CAYNNNNRTG 1 cut(s) 60
MspI CCGG 1 cut(s) 127
MspR9I CCNGG 1 cut(s) 7
MvaI CCWGG 1 cut(s) 7
NdeI CATATG 1 cut(s) 242
NdeII GATC 2 cut(s) 132, 269
NlaIII CATG 3 cut(s) 178, 253, 265
NlaIV GGNNCC 3 cut(s) 134, 204, 227
NmeAIII GCCGAG 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 47
NspI RCATGY 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 5
PinAI ACCGGT 1 cut(s) 126
PkrI GCNGC 1 cut(s) 248
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 5
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 3 cut(s) 134, 204, 227
PspPI GGNCC 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 164, 196
PsuI RGATCY 1 cut(s) 132
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
RseI CAYNNNNRTG 1 cut(s) 60
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 2 cut(s) 132, 269
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 7
SduI GDGCHC 2 cut(s) 369, 434
SetI ASST 6 cut(s) 60, 168, 186, 192, 412, 427
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 3 cut(s) 17, 236, 311
SsiI CCGC 1 cut(s) 88
SspMI CTAG 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 292
TaqI TCGA 2 cut(s) 214, 282
TasI AATT 3 cut(s) 17, 236, 311
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 3 cut(s) 229, 266, 268
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 2 cut(s) 236, 311
XceI RCATGY 1 cut(s) 265
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.