MD14G1201800.v1.1

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009773
Physical Location & Seq
Forward (+)
29079690 .. 29081986
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1201800.v1.1.491

Sequence Viewer

Length: 777 bp
ATGAACCTTATCAAATTAAAAGGAATTCCCATTCTCCAACAGCTTCATTTAGAGGAGCAACTTCTGCGGACCTCGTCCCATAATTGGTGCATTGTAAATGATGGAACTAATGATCCCACTATTGTCATGGGTGTTTCGGGAAAACCTGCAGAGCTTCTTGAAATTGATTTGGTGTTACGAGACCAGATTCCTGTCATTAGAAGGTTTACTGGAGGTGGCACTGTTACTGTTGATCAAAATACGCTGTTTGTCACTTTCATATGCAACAAGGATGCCGTTCCTGGCTTGCAACCATATCCTCGACCTATAATGTCCTGGAGTAGCCTAGTTTACAGCAAAGTGTTTGAAGGACTTGCTGATTTTCAACTTCGTGAAAATGATTATGTATTTGGAAACCGCAAGTTTGGTGGGAACGCTCAATCTATTAGTAAAAACCGGTGGATCCACCACACTTCCTTTCTATGGGACTATGATGTTAGGAACATGGCATACCTGAAGCATCCAAAACGGGTTCCTGAATATCGATTGACAAGAGATCATTTAGAATTCATATGCTGCATGAAAGACTACATCCCAAGATCAGTTTTTCTTGAGAAAACAGTCGAGGCACTCTTATCCCAATTCTCCGTGAGATCGGAACGGTCGGACGCCATTGAAGCTACCTCGAGTACAAAGTTTGTACCCTCAACTAGGCTGTTGACAAGGCAGGAATTAGAGGAAGCAGCAGCTTTTGACTCTCAGGCTGAAAGAAATCTCTCTCAACCATTGTCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.55

Weight (kDa)

7.01

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 11 - 208 1.5e-30 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AciI CCGC 2 cut(s) 67, 397
AclWI GGATC 3 cut(s) 107, 436, 449
AcsI RAATTY 2 cut(s) 24, 545
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 648
AfaI GTAC 2 cut(s) 670, 681
AfiI CCNNNNNNNGG 3 cut(s) 84, 462, 690
AgeI ACCGGT 1 cut(s) 435
AgsI TTSAA 4 cut(s) 161, 347, 365, 656
AjnI CCWGG 2 cut(s) 280, 314
AloI GAACNNNNNNTCC 2 cut(s) 97, 129
AluBI AGCT 4 cut(s) 43, 154, 659, 728
AluI AGCT 4 cut(s) 43, 154, 659, 728
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 107, 436, 449
Ama87I CYCGRG 1 cut(s) 664
ApeKI GCWGC 3 cut(s) 555, 722, 725
ApoI RAATTY 2 cut(s) 24, 545
AsiGI ACCGGT 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 69
AvaI CYCGRG 1 cut(s) 664
AvaII GGWCC 1 cut(s) 69
BamHI GGATCC 1 cut(s) 441
BbvI GCAGC 3 cut(s) 542, 734, 737
BccI CCATC 1 cut(s) 95
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 2 cut(s) 282, 316
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 2 cut(s) 326, 690
BfmI CTRYAG 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 3 cut(s) 556, 723, 726
BlsI GCNGC 3 cut(s) 557, 724, 727
Bme1390I CCNGG 2 cut(s) 282, 316
Bme18I GGWCC 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 664
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 2 cut(s) 443, 513
BmrFI CCNGG 2 cut(s) 282, 316
BmsI GCATC 2 cut(s) 262, 508
BpmI CTGGAG 2 cut(s) 231, 337
BpuEI CTTGAG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 523
BsaHI GRCGYC 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 174
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 462, 690
Bse118I RCCGGY 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 282, 316
BseCI ATCGAT 1 cut(s) 523
BseGI GGATG 3 cut(s) 277, 499, 570
BseLI CCNNNNNNNGG 3 cut(s) 84, 462, 690
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 542, 734, 737
Bsh1285I CGRYCG 1 cut(s) 644
BshTI ACCGGT 1 cut(s) 435
BshVI ATCGAT 1 cut(s) 523
BsiEI CGRYCG 1 cut(s) 644
BsiHKCI CYCGRG 1 cut(s) 664
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 2 cut(s) 61, 479
BslI CCNNNNNNNGG 3 cut(s) 84, 462, 690
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 61, 479
Bso31I GGTCTC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 664
Bsp143I GATC 6 cut(s) 112, 232, 441, 535, 578, 632
BspACI CCGC 2 cut(s) 67, 397
BspCNI CTCAG 1 cut(s) 751
BspDI ATCGAT 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 443, 513
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 3 cut(s) 107, 436, 449
BspTNI GGTCTC 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 435
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 435
BssMI GATC 6 cut(s) 112, 232, 441, 535, 578, 632
BssNI GRCGYC 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 282, 316
Bst4CI ACNGT 4 cut(s) 223, 229, 601, 642
BstACI GRCGYC 1 cut(s) 648
BstAPI GCANNNNNTGC 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 1 cut(s) 738
BstENI CCTNNNNNAGG 1 cut(s) 688
BstF5I GGATG 3 cut(s) 277, 499, 570
BstKTI GATC 6 cut(s) 115, 235, 444, 538, 581, 635
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 6 cut(s) 112, 232, 441, 535, 578, 632
BstMCI CGRYCG 1 cut(s) 644
BstMWI GCNNNNNNNGC 2 cut(s) 64, 656
BstNI CCWGG 2 cut(s) 282, 316
BstSCI CCNGG 2 cut(s) 280, 314
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 542, 734, 737
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 523
BsuTUI ATCGAT 1 cut(s) 523
BtsCI GGATG 3 cut(s) 277, 499, 570
BtsIMutI CAGTG 1 cut(s) 219
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 1 cut(s) 287
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 1 cut(s) 69
ClaI ATCGAT 1 cut(s) 523
CseI GACGC 1 cut(s) 656
Csp6I GTAC 2 cut(s) 669, 680
CspAI ACCGGT 1 cut(s) 435
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 3 cut(s) 127, 484, 559
CviJI RGCY 8 cut(s) 43, 154, 285, 324, 659, 694, 728, 743
CviKI_1 RGCY 8 cut(s) 43, 154, 285, 324, 659, 694, 728, 743
CviQI GTAC 2 cut(s) 669, 680
DdeI CTNAG 1 cut(s) 738
DpnI GATC 6 cut(s) 114, 234, 443, 537, 580, 634
DpnII GATC 6 cut(s) 112, 232, 441, 535, 578, 632
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 688
EcoRI GAATTC 2 cut(s) 24, 545
EcoRII CCWGG 2 cut(s) 280, 314
FaeI CATG 3 cut(s) 130, 487, 562
FaqI GGGAC 2 cut(s) 61, 479
FatI CATG 3 cut(s) 126, 483, 558
FauNDI CATATG 2 cut(s) 260, 551
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 3 cut(s) 556, 723, 726
FokI GGATG 3 cut(s) 284, 486, 557
Fsp4HI GCNGC 3 cut(s) 556, 723, 726
FspBI CTAG 2 cut(s) 326, 690
GluI GCNGC 3 cut(s) 556, 723, 726
GsuI CTGGAG 2 cut(s) 231, 337
HapII CCGG 1 cut(s) 436
HgaI GACGC 1 cut(s) 656
Hin1I GRCGYC 1 cut(s) 648
Hin1II CATG 3 cut(s) 130, 487, 562
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HinfI GANTC 2 cut(s) 187, 734
HpaII CCGG 1 cut(s) 436
Hpy166II GTNNAC 3 cut(s) 207, 331, 699
Hpy188I TCNGA 2 cut(s) 637, 646
Hpy188III TCNNGA 5 cut(s) 138, 158, 371, 515, 590
Hpy8I GTNNAC 3 cut(s) 207, 331, 699
HpyAV CCTTC 2 cut(s) 195, 341
HpyCH4III ACNGT 4 cut(s) 223, 229, 601, 642
HpyCH4V TGCA 5 cut(s) 90, 149, 264, 289, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 656
HpyF3I CTNAG 1 cut(s) 738
Hsp92I GRCGYC 1 cut(s) 648
Hsp92II CATG 3 cut(s) 130, 487, 562
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 6 cut(s) 112, 232, 441, 535, 578, 632
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 3 cut(s) 542, 734, 737
LweI GCATC 2 cut(s) 262, 508
MaeI CTAG 2 cut(s) 326, 690
MaeIII GTNAC 3 cut(s) 174, 223, 250
MalI GATC 6 cut(s) 114, 234, 443, 537, 580, 634
MboI GATC 6 cut(s) 112, 232, 441, 535, 578, 632
MflI RGATCY 1 cut(s) 441
MluCI AATT 7 cut(s) 14, 24, 82, 162, 545, 620, 710
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 2 cut(s) 61, 624
MnlI CCTC 8 cut(s) 46, 82, 206, 309, 598, 673, 694, 709
MseI TTAA 1 cut(s) 17
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 2 cut(s) 282, 316
MvaI CCWGG 2 cut(s) 282, 316
MwoI GCNNNNNNNGC 2 cut(s) 64, 656
NdeI CATATG 2 cut(s) 260, 551
NdeII GATC 6 cut(s) 112, 232, 441, 535, 578, 632
NlaIII CATG 3 cut(s) 130, 487, 562
NlaIV GGNNCC 2 cut(s) 443, 513
NmuCI GTSAC 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 664
PfeI GAWTC 1 cut(s) 187
PflFI GACNNNGTC 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 435
PkrI GCNGC 3 cut(s) 557, 724, 727
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 280, 314
PspGI CCWGG 2 cut(s) 280, 314
PspN4I GGNNCC 2 cut(s) 443, 513
PspPI GGNCC 1 cut(s) 69
PspXI VCTCGAGB 1 cut(s) 664
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 441
PsyI GACNNNGTC 1 cut(s) 73
RsaI GTAC 2 cut(s) 670, 681
RsaNI GTAC 2 cut(s) 669, 680
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 3 cut(s) 556, 723, 726
Sau3AI GATC 6 cut(s) 112, 232, 441, 535, 578, 632
Sau96I GGNCC 1 cut(s) 69
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 2 cut(s) 282, 316
SfaNI GCATC 2 cut(s) 262, 508
SfcI CTRYAG 1 cut(s) 147
Sfr274I CTCGAG 1 cut(s) 664
SinI GGWCC 1 cut(s) 69
SlaI CTCGAG 1 cut(s) 664
SmlI CTYRAG 2 cut(s) 590, 664
SmoI CTYRAG 2 cut(s) 590, 664
Sse9I AATT 7 cut(s) 14, 24, 82, 162, 545, 620, 710
SsiI CCGC 2 cut(s) 67, 397
SspMI CTAG 2 cut(s) 326, 690
StyD4I CCNGG 2 cut(s) 280, 314
TaaI ACNGT 4 cut(s) 223, 229, 601, 642
TaqI TCGA 4 cut(s) 301, 523, 603, 665
TasI AATT 7 cut(s) 14, 24, 82, 162, 545, 620, 710
TatI WGTACW 1 cut(s) 668
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 3 cut(s) 555, 722, 725
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 17, 35, 247, 538, 575
TspGWI ACGGA 1 cut(s) 616
TspRI CASTG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 69
XagI CCTNNNNNAGG 1 cut(s) 688
XapI RAATTY 2 cut(s) 24, 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 664
XspI CTAG 2 cut(s) 326, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.