RchiOBHm_Chr7g0181591

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009773
Physical Location & Seq
Forward (+)
3192946 .. 3195177
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16197

Sequence Viewer

Length: 825 bp
ATGACTATACCTCAAACAAGAAACGTTGGGCTTCCATTGATGAACCTTATCAGATTAAAAGGAATTCCCATCCTGCAGCAGCTTCATTTAGAGGAGCAACTGCTTCGAACCTCGTATGATAATTGGTGCATTATAAATGATGGAACCAATAGTCCTAATATTGTCATGGGTATTTCAGGAAAGCCTGCAGAGCTTCTTGACGTTGATCTGGTGTTACGAGATCAGGTCCCTGTCATTAAAAGGTTTACTGGAGGTGGCACGGTTACAGTTGATAATGGCACGATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTTGCTTGCAACTATATCCGCGACCTATAATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAGGATTTGGTGACTTTCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAATCTATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGTTCCTGAATATCGATTGGCAAGGGACCATTTGGAATTCATATGCCGCATGAAAGACTACATGCCAAGATCAGTTTTCATCGACAAAACCGTCGAGGCAATCAGTACCGAATTTTCAGTCAAATCTGAACAAACAGACCCATTTAAAACCAACTCAAAGTTTGTGCCCTCAACTAGACTATTGACAAGGCAAGAGTTGGAAGAAGTAGCTTTGGGCTCTCAACCTGAGAGCCCTCTCTCTGAGAGTCTGAGTCCCTGTCCTTGTAATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

31.43

Weight (kDa)

7.62

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 24 - 221 3.2e-29 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
AasI GACNNNNNNGTC 1 cut(s) 638
AccII CGCG 1 cut(s) 340
AciI CCGC 3 cut(s) 338, 436, 595
AclI AACGTT 1 cut(s) 24
AclWI GGATC 2 cut(s) 475, 488
AcsI RAATTY 3 cut(s) 63, 584, 659
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 1 cut(s) 655
AfiI CCNNNNNNNGG 1 cut(s) 501
AgeI ACCGGT 1 cut(s) 474
AjnI CCWGG 1 cut(s) 353
AleI CACNNNNGTG 1 cut(s) 408
AloI GAACNNNNNNTCC 2 cut(s) 136, 168
AluBI AGCT 4 cut(s) 82, 193, 538, 758
AluI AGCT 4 cut(s) 82, 193, 538, 758
AlwI GGATC 2 cut(s) 475, 488
ApeKI GCWGC 2 cut(s) 76, 79
ApoI RAATTY 3 cut(s) 63, 584, 659
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
AsiGI ACCGGT 1 cut(s) 474
AspS9I GGNCC 2 cut(s) 226, 574
AsuHPI GGTGA 2 cut(s) 395, 407
AsuII TTCGAA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 226, 574
BaeGI GKGCMC 1 cut(s) 717
BamHI GGATCC 1 cut(s) 480
BanII GRGCYC 2 cut(s) 767, 782
BbvI GCAGC 2 cut(s) 88, 91
BccI CCATC 2 cut(s) 77, 134
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 355
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 2 cut(s) 74, 186
BisI GCNGC 3 cut(s) 77, 80, 595
BlsI GCNGC 3 cut(s) 78, 81, 596
Bme1390I CCNGG 1 cut(s) 355
Bme18I GGWCC 2 cut(s) 226, 574
BmgT120I GGNCC 2 cut(s) 226, 574
BmiI GGNNCC 5 cut(s) 145, 228, 482, 552, 575
BmrFI CCNGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 301
BpmI CTGGAG 2 cut(s) 270, 376
Bpu14I TTCGAA 1 cut(s) 106
Bsa29I ATCGAT 1 cut(s) 562
BsaJI CCNNGG 1 cut(s) 385
BsaWI WCCGGW 1 cut(s) 474
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 474
Bse1I ACTGG 1 cut(s) 253
BseBI CCWGG 1 cut(s) 355
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 3 cut(s) 69, 316, 491
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 765, 780, 788
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 717
BseXI GCAGC 2 cut(s) 88, 91
Bsh1236I CGCG 1 cut(s) 340
BshTI ACCGGT 1 cut(s) 474
BshVI ATCGAT 1 cut(s) 562
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 4 cut(s) 212, 518, 587, 786
BslI CCNNNNNNNGG 1 cut(s) 501
BsmFI GGGAC 4 cut(s) 212, 518, 587, 786
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 3 cut(s) 717, 767, 782
Bsp143I GATC 4 cut(s) 205, 220, 480, 617
BspACI CCGC 3 cut(s) 338, 436, 595
BspCNI CTCAG 3 cut(s) 766, 781, 789
BspDI ATCGAT 1 cut(s) 562
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 5 cut(s) 145, 228, 482, 552, 575
BspMAI CTGCAG 2 cut(s) 78, 190
BspPI GGATC 2 cut(s) 475, 488
BspT104I TTCGAA 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 474
BsrI ACTGG 1 cut(s) 253
BssAI RCCGGY 1 cut(s) 474
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 4 cut(s) 205, 220, 480, 617
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 3 cut(s) 262, 268, 640
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 186, 326
BstDEI CTNAG 3 cut(s) 774, 789, 797
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 3 cut(s) 69, 316, 491
BstFNI CGCG 1 cut(s) 340
BstKTI GATC 4 cut(s) 208, 223, 483, 620
BstMBI GATC 4 cut(s) 205, 220, 480, 617
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 613
BstPI GGTNACC 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 74, 186
BstSLI GKGCMC 1 cut(s) 717
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 88, 91
BstX2I RGATCY 1 cut(s) 480
BstXI CCANNNNNNTGG 1 cut(s) 392
BstYI RGATCY 1 cut(s) 480
Bsu15I ATCGAT 1 cut(s) 562
BsuTUI ATCGAT 1 cut(s) 562
BtsCI GGATG 3 cut(s) 69, 316, 491
Cac8I GCNNGC 2 cut(s) 186, 326
Cfr10I RCCGGY 1 cut(s) 474
Cfr13I GGNCC 2 cut(s) 226, 574
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 1 cut(s) 654
CspAI ACCGGT 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 4 cut(s) 166, 523, 598, 610
CviJI RGCY 9 cut(s) 31, 82, 184, 193, 363, 538, 758, 765, 780
CviKI_1 RGCY 9 cut(s) 31, 82, 184, 193, 363, 538, 758, 765, 780
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 3 cut(s) 774, 789, 797
DpnI GATC 4 cut(s) 207, 222, 482, 619
DpnII GATC 4 cut(s) 205, 220, 480, 617
DraI TTTAAA 1 cut(s) 694
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 2 cut(s) 767, 782
Eco47I GGWCC 2 cut(s) 226, 574
Eco57I CTGAAG 1 cut(s) 554
Eco91I GGTNACC 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 431
EcoRI GAATTC 2 cut(s) 63, 584
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 2 cut(s) 767, 782
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 4 cut(s) 169, 526, 601, 613
FaqI GGGAC 4 cut(s) 212, 518, 587, 786
FatI CATG 4 cut(s) 165, 522, 597, 609
FauNDI CATATG 2 cut(s) 299, 590
Fnu4HI GCNGC 3 cut(s) 77, 80, 595
FokI GGATG 3 cut(s) 56, 323, 478
FriOI GRGCYC 2 cut(s) 767, 782
Fsp4HI GCNGC 3 cut(s) 77, 80, 595
FspBI CTAG 1 cut(s) 723
GluI GCNGC 3 cut(s) 77, 80, 595
GsuI CTGGAG 2 cut(s) 270, 376
HapII CCGG 1 cut(s) 475
Hin1II CATG 4 cut(s) 169, 526, 601, 613
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 793, 799
HpaII CCGG 1 cut(s) 475
HphI GGTGA 2 cut(s) 395, 407
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 4 cut(s) 53, 676, 790, 798
Hpy188III TCNNGA 4 cut(s) 177, 197, 410, 554
Hpy8I GTNNAC 1 cut(s) 246
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 3 cut(s) 262, 268, 640
HpyCH4IV ACGT 2 cut(s) 24, 201
HpyCH4V TGCA 5 cut(s) 76, 129, 188, 303, 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 3 cut(s) 774, 789, 797
HpySE526I ACGT 2 cut(s) 24, 201
Hsp92II CATG 4 cut(s) 169, 526, 601, 613
Kzo9I GATC 4 cut(s) 205, 220, 480, 617
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 2 cut(s) 88, 91
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 723
MaeII ACGT 2 cut(s) 24, 201
MaeIII GTNAC 5 cut(s) 213, 262, 289, 395, 431
MalI GATC 4 cut(s) 207, 222, 482, 619
MboI GATC 4 cut(s) 205, 220, 480, 617
MboII GAAGA 1 cut(s) 761
MflI RGATCY 1 cut(s) 480
MhlI GDGCHC 3 cut(s) 717, 767, 782
MluCI AATT 5 cut(s) 63, 121, 365, 584, 659
MlyI GAGTC 2 cut(s) 802, 808
MmeI TCCRAC 1 cut(s) 726
MnlI CCTC 8 cut(s) 21, 85, 121, 245, 505, 637, 727, 792
MseI TTAA 3 cut(s) 56, 237, 693
MslI CAYNNNNRTG 1 cut(s) 408
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 1 cut(s) 355
MvaI CCWGG 1 cut(s) 355
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeI CATATG 2 cut(s) 299, 590
NdeII GATC 4 cut(s) 205, 220, 480, 617
NlaIII CATG 4 cut(s) 169, 526, 601, 613
NlaIV GGNNCC 5 cut(s) 145, 228, 482, 552, 575
NmuCI GTSAC 2 cut(s) 289, 395
NspI RCATGY 1 cut(s) 613
NspV TTCGAA 1 cut(s) 106
OliI CACNNNNGTG 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfoI TCCNGGA 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 474
PkrI GCNGC 3 cut(s) 78, 81, 596
PleI GAGTC 2 cut(s) 801, 807
PpsI GAGTC 2 cut(s) 801, 807
PpuMI RGGWCCY 1 cut(s) 226
PsiI TTATAA 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 353
PspEI GGTNACC 1 cut(s) 431
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 5 cut(s) 145, 228, 482, 552, 575
PspPI GGNCC 2 cut(s) 226, 574
PspPPI RGGWCCY 1 cut(s) 226
PsrI GAACNNNNNNTAC 2 cut(s) 512, 544
PstI CTGCAG 2 cut(s) 78, 190
PsuI RGATCY 1 cut(s) 480
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 3 cut(s) 56, 237, 693
SatI GCNGC 3 cut(s) 77, 80, 595
Sau3AI GATC 4 cut(s) 205, 220, 480, 617
Sau96I GGNCC 2 cut(s) 226, 574
SchI GAGTC 2 cut(s) 802, 808
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 3 cut(s) 717, 767, 782
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 2 cut(s) 74, 186
SfuI TTCGAA 1 cut(s) 106
SinI GGWCC 2 cut(s) 226, 574
SmiMI CAYNNNNRTG 1 cut(s) 408
Sse9I AATT 5 cut(s) 63, 121, 365, 584, 659
SsiI CCGC 3 cut(s) 338, 436, 595
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 723
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 3 cut(s) 262, 268, 640
TaiI ACGT 2 cut(s) 27, 204
TaqI TCGA 4 cut(s) 106, 562, 630, 642
TasI AATT 5 cut(s) 63, 121, 365, 584, 659
TauI GCSGC 1 cut(s) 597
Tru1I TTAA 3 cut(s) 56, 237, 693
Tru9I TTAA 3 cut(s) 56, 237, 693
TseFI GTSAC 2 cut(s) 289, 395
TseI GCWGC 2 cut(s) 76, 79
Tsp45I GTSAC 2 cut(s) 289, 395
TspDTI ATGAA 6 cut(s) 56, 74, 286, 577, 614, 616
VpaK11BI GGWCC 2 cut(s) 226, 574
XapI RAATTY 3 cut(s) 63, 584, 659
XceI RCATGY 1 cut(s) 613
XspI CTAG 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.