RLG00000005181

Lipoate-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009773
Physical Location & Seq
Reverse (-)
65678224 .. 65682684
4461 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005181

Sequence Viewer

Length: 1326 bp
ATGGAGAAGACATTAATGGATGAAGCAATTGCCAGCAGCTCCATTGCACTTCTTATGGATGAAGCAATTGCCAGCAGCTCCATTGCACTCGTTGGACTAGTTCTGTTTTTCTTAATGATTATTAAGTGGCTCATTGTGCAATTCTTGCTACACTTGTCAAGTTTAAGGTATGCACTTCAATTCTTTGAAATTAATTGTGGTGTGAACAATGTTAACCTCGATGATCAGACCAAGATCACAACCGAGAAAGCTTGCACTTACAAAGTAGGTGTCGAAGAGAACACGAAAGACATCGGTGAAAAGAAACTGTGTTTAGGAGAATTAGCATCGGTTATGGAGAACCTGGGAAGTTTATGCGGTGATAATGGGTCTAAGGAGATGGCTGACTTGCTTGAGGAGGAACCCAGCTTGGAGGAAGTAAAAGAAGCCTTCCATTTGTTTGATGAAAATACCGATGGGTTCATCGATGCAGGGGAGATCAAGAAGATTTCAGTCGACAGCGGAAACAGGATGACTGTACCTCAAACAAGAAACGTTGGGCTTCCATTGATGAACCTTATCAGATTAAAAGGAATTCCCATCCTGCAGCAGCTTCATTTAGAGGAGCAACTGCTTCGAACCTCGTATGATAATTGGTGCATTATAAATGATGGAACCAATAGTCCTAATATTGTCATGGGTATTTCAGGAAAGCCTGCAGAGCTTCTTGACGTTGATCTGGTGTTACGAGATCAGGTTCCTGTCATTAAAAGGTTTACTGGAGGTGGCACGGTTACAGTTGATAATGGCACAATATTTGTCACTTTCATATGCAACAGGGATGCTGTTCCTGGCTTGCAACTATATCCGCGACCTATAATGTCCTGGAGTAGCCAATTATACAGCAGAGTGTTCCAAGGATTTGGTGACTTTCACCTTCGTGAAAATGATTATGTATTTGGTAACCGCAAGTTTGGTGGTAATGCTCAATCTATTACTAAAAACCGGTGGATCCACCACACATCCTTTCTATGGGACTATGAGGTTAGGAACATGGCATACCTGAAGCTTCCAAAACGGGTTCCTGAGTATCGATTGGCAAGGGACCATTTGGAATTCATATGCCGCATGAAGGACTCCATGCCAAGATCAGTTTTCATCGACAAAACTGTCGAGGCAATCAGTACCGAATTTTCAGTAAACTCAGAAGAAACAGACCCATTTGGAACCAACTCAAAGTTTGTGCCGTCAACTAGACTATTGACAAGGCAAGAGTTGGAAGAAGTAGCTTTAGCCTCTCAACCTGAGAGCCCTCTCTCTCAATCCTTGTCTTGTAATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

49.64

Weight (kDa)

4.98

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LplA-B_cat PF21948 194 - 391 7.2e-28 Lipoyl protein ligase A/B catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AasI GACNNNNNNGTC 1 cut(s) 1148
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 850
AciI CCGC 5 cut(s) 357, 501, 848, 946, 1105
AclI AACGTT 1 cut(s) 534
AclWI GGATC 2 cut(s) 985, 998
AcsI RAATTY 3 cut(s) 573, 1094, 1169
AcuI CTGAAG 1 cut(s) 1064
AfaI GTAC 2 cut(s) 519, 1165
AfiI CCNNNNNNNGG 2 cut(s) 1011, 1111
AgeI ACCGGT 1 cut(s) 984
AgsI TTSAA 2 cut(s) 179, 188
AhlI ACTAGT 1 cut(s) 97
AjnI CCWGG 3 cut(s) 342, 829, 863
AleI CACNNNNGTG 1 cut(s) 918
AloI GAACNNNNNNTCC 2 cut(s) 646, 678
AluBI AGCT 8 cut(s) 39, 78, 251, 408, 592, 703, 1048, 1268
AluI AGCT 8 cut(s) 39, 78, 251, 408, 592, 703, 1048, 1268
AlwI GGATC 2 cut(s) 985, 998
ApeKI GCWGC 4 cut(s) 36, 75, 586, 589
ApoI RAATTY 3 cut(s) 573, 1094, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 255, 287
AseI ATTAAT 2 cut(s) 14, 192
AsiGI ACCGGT 1 cut(s) 984
AspS9I GGNCC 1 cut(s) 1084
AsuHPI GGTGA 4 cut(s) 308, 371, 905, 917
AsuII TTCGAA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 1084
BamHI GGATCC 1 cut(s) 990
BanII GRGCYC 1 cut(s) 1292
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 4 cut(s) 48, 87, 598, 601
BccI CCATC 4 cut(s) 373, 449, 587, 644
BceAI ACGGC 1 cut(s) 1210
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 3 cut(s) 344, 831, 865
BclI TGATCA 1 cut(s) 223
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 2 cut(s) 98, 1233
BfmI CTRYAG 2 cut(s) 584, 696
BisI GCNGC 5 cut(s) 37, 76, 587, 590, 1105
BlsI GCNGC 5 cut(s) 38, 77, 588, 591, 1106
Bme1390I CCNGG 3 cut(s) 344, 831, 865
Bme18I GGWCC 1 cut(s) 1084
BmgT120I GGNCC 1 cut(s) 1084
BmiI GGNNCC 7 cut(s) 402, 655, 738, 992, 1062, 1085, 1207
BmrFI CCNGG 3 cut(s) 344, 831, 865
BmsI GCATC 3 cut(s) 335, 457, 811
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 2 cut(s) 780, 886
Bpu14I TTCGAA 1 cut(s) 616
BpuEI CTTGAG 1 cut(s) 413
Bsa29I ATCGAT 2 cut(s) 465, 1072
BsaJI CCNNGG 2 cut(s) 343, 895
BsaWI WCCGGW 1 cut(s) 984
Bsc4I CCNNNNNNNGG 2 cut(s) 1011, 1111
Bse118I RCCGGY 1 cut(s) 984
Bse1I ACTGG 1 cut(s) 763
Bse3DI GCAATG 2 cut(s) 42, 81
BseBI CCWGG 3 cut(s) 344, 831, 865
BseCI ATCGAT 2 cut(s) 465, 1072
BseDI CCNNGG 2 cut(s) 343, 895
BseGI GGATG 6 cut(s) 25, 64, 516, 579, 826, 1001
BseLI CCNNNNNNNGG 2 cut(s) 1011, 1111
BseMI GCAATG 2 cut(s) 42, 81
BseMII CTCAG 3 cut(s) 1056, 1197, 1275
BseNI ACTGG 1 cut(s) 763
BseRI GAGGAG 2 cut(s) 410, 617
BseXI GCAGC 4 cut(s) 48, 87, 598, 601
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 1 cut(s) 850
BshTI ACCGGT 1 cut(s) 984
BshVI ATCGAT 2 cut(s) 465, 1072
BsiSI CCGG 1 cut(s) 985
BslFI GGGAC 2 cut(s) 1028, 1097
BslI CCNNNNNNNGG 2 cut(s) 1011, 1111
BsmFI GGGAC 2 cut(s) 1028, 1097
Bsp119I TTCGAA 1 cut(s) 616
Bsp1286I GDGCHC 1 cut(s) 1292
Bsp143I GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
BspACI CCGC 5 cut(s) 357, 501, 848, 946, 1105
BspCNI CTCAG 3 cut(s) 1057, 1196, 1276
BspDI ATCGAT 2 cut(s) 465, 1072
BspFNI CGCG 1 cut(s) 850
BspLI GGNNCC 7 cut(s) 402, 655, 738, 992, 1062, 1085, 1207
BspMAI CTGCAG 2 cut(s) 588, 700
BspPI GGATC 2 cut(s) 985, 998
BspT104I TTCGAA 1 cut(s) 616
BsrDI GCAATG 2 cut(s) 42, 81
BsrFI RCCGGY 1 cut(s) 984
BsrI ACTGG 1 cut(s) 763
BssAI RCCGGY 1 cut(s) 984
BssECI CCNNGG 2 cut(s) 343, 895
BssMI GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
BssT1I CCWWGG 1 cut(s) 895
Bst2UI CCWGG 3 cut(s) 344, 831, 865
Bst4CI ACNGT 5 cut(s) 309, 517, 772, 778, 1150
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 145
BstBI TTCGAA 1 cut(s) 616
BstC8I GCNNGC 5 cut(s) 34, 73, 253, 696, 836
BstDEI CTNAG 4 cut(s) 372, 1065, 1183, 1284
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 6 cut(s) 25, 64, 516, 579, 826, 1001
BstFNI CGCG 1 cut(s) 850
BstKTI GATC 7 cut(s) 226, 237, 480, 718, 733, 993, 1130
BstMBI GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
BstMWI GCNNNNNNNGC 3 cut(s) 136, 145, 700
BstNI CCWGG 3 cut(s) 344, 831, 865
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 342, 829, 863
BstSFI CTRYAG 2 cut(s) 584, 696
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 4 cut(s) 48, 87, 598, 601
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 990
BstXI CCANNNNNNTGG 1 cut(s) 902
BstYI RGATCY 1 cut(s) 990
Bsu15I ATCGAT 2 cut(s) 465, 1072
BsuTUI ATCGAT 2 cut(s) 465, 1072
BtsCI GGATG 6 cut(s) 25, 64, 516, 579, 826, 1001
Cac8I GCNNGC 5 cut(s) 34, 73, 253, 696, 836
Cfr10I RCCGGY 1 cut(s) 984
Cfr13I GGNCC 1 cut(s) 1084
ClaI ATCGAT 2 cut(s) 465, 1072
Csp6I GTAC 2 cut(s) 518, 1164
CspAI ACCGGT 1 cut(s) 984
CspCI CAANNNNNGTGG 2 cut(s) 937, 972
CviAII CATG 4 cut(s) 676, 1033, 1108, 1120
CviQI GTAC 2 cut(s) 518, 1164
DdeI CTNAG 4 cut(s) 372, 1065, 1183, 1284
DpnI GATC 7 cut(s) 225, 236, 479, 717, 732, 992, 1129
DpnII GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
DrdI GACNNNNNNGTC 1 cut(s) 1148
DseDI GACNNNNNNGTC 1 cut(s) 1148
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 895
Eco24I GRGCYC 1 cut(s) 1292
Eco47I GGWCC 1 cut(s) 1084
Eco57I CTGAAG 1 cut(s) 1064
Eco91I GGTNACC 1 cut(s) 941
EcoO65I GGTNACC 1 cut(s) 941
EcoRI GAATTC 2 cut(s) 573, 1094
EcoRII CCWGG 3 cut(s) 342, 829, 863
EcoT14I CCWWGG 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 1292
ErhI CCWWGG 1 cut(s) 895
FaeI CATG 4 cut(s) 679, 1036, 1111, 1123
FaqI GGGAC 2 cut(s) 1028, 1097
FatI CATG 4 cut(s) 675, 1032, 1107, 1119
FauNDI CATATG 2 cut(s) 809, 1100
FbaI TGATCA 1 cut(s) 223
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 5 cut(s) 37, 76, 587, 590, 1105
FokI GGATG 6 cut(s) 32, 71, 523, 566, 833, 988
FriOI GRGCYC 1 cut(s) 1292
Fsp4HI GCNGC 5 cut(s) 37, 76, 587, 590, 1105
FspBI CTAG 2 cut(s) 98, 1233
GluI GCNGC 5 cut(s) 37, 76, 587, 590, 1105
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 2 cut(s) 780, 886
HapII CCGG 1 cut(s) 985
Hin1II CATG 4 cut(s) 679, 1036, 1111, 1123
HincII GTYRAC 3 cut(s) 214, 496, 1230
HindII GTYRAC 3 cut(s) 214, 496, 1230
HindIII AAGCTT 2 cut(s) 249, 1046
HinfI GANTC 1 cut(s) 1115
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 1 cut(s) 985
HphI GGTGA 4 cut(s) 308, 371, 905, 917
Hpy166II GTNNAC 6 cut(s) 205, 214, 496, 756, 1180, 1230
Hpy188I TCNGA 3 cut(s) 228, 563, 1186
Hpy188III TCNNGA 5 cut(s) 481, 687, 707, 920, 1064
Hpy8I GTNNAC 6 cut(s) 205, 214, 496, 756, 1180, 1230
HpyAV CCTTC 3 cut(s) 439, 926, 1105
HpyCH4III ACNGT 5 cut(s) 309, 517, 772, 778, 1150
HpyCH4IV ACGT 2 cut(s) 534, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 145, 700
HpyF3I CTNAG 4 cut(s) 372, 1065, 1183, 1284
HpySE526I ACGT 2 cut(s) 534, 711
Hsp92II CATG 4 cut(s) 679, 1036, 1111, 1123
Ksp22I TGATCA 1 cut(s) 223
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
LmnI GCTCC 3 cut(s) 44, 83, 604
Lsp1109I GCAGC 4 cut(s) 48, 87, 598, 601
LweI GCATC 3 cut(s) 335, 457, 811
MaeI CTAG 2 cut(s) 98, 1233
MaeII ACGT 2 cut(s) 534, 711
MaeIII GTNAC 5 cut(s) 723, 772, 799, 905, 941
MalI GATC 7 cut(s) 225, 236, 479, 717, 732, 992, 1129
MboI GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
MboII GAAGA 5 cut(s) 19, 287, 496, 1199, 1271
MfeI CAATTG 2 cut(s) 27, 66
MflI RGATCY 1 cut(s) 990
MhlI GDGCHC 1 cut(s) 1292
MlyI GAGTC 1 cut(s) 1109
MmeI TCCRAC 2 cut(s) 73, 1236
MseI TTAA 8 cut(s) 14, 113, 123, 164, 192, 213, 566, 747
MslI CAYNNNNRTG 1 cut(s) 918
MspA1I CMGCKG 1 cut(s) 501
MspI CCGG 1 cut(s) 985
MspR9I CCNGG 3 cut(s) 344, 831, 865
MunI CAATTG 2 cut(s) 27, 66
MvaI CCWGG 3 cut(s) 344, 831, 865
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 3 cut(s) 136, 145, 700
NdeI CATATG 2 cut(s) 809, 1100
NdeII GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
NlaIII CATG 4 cut(s) 679, 1036, 1111, 1123
NlaIV GGNNCC 7 cut(s) 402, 655, 738, 992, 1062, 1085, 1207
NmuCI GTSAC 2 cut(s) 799, 905
NspV TTCGAA 1 cut(s) 616
OliI CACNNNNGTG 1 cut(s) 918
PfoI TCCNGGA 1 cut(s) 863
PinAI ACCGGT 1 cut(s) 984
PkrI GCNGC 5 cut(s) 38, 77, 588, 591, 1106
PleI GAGTC 1 cut(s) 1109
PpsI GAGTC 1 cut(s) 1109
PshBI ATTAAT 2 cut(s) 14, 192
PsiI TTATAA 1 cut(s) 644
Psp1406I AACGTT 1 cut(s) 534
Psp6I CCWGG 3 cut(s) 342, 829, 863
PspEI GGTNACC 1 cut(s) 941
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 3 cut(s) 342, 829, 863
PspN4I GGNNCC 7 cut(s) 402, 655, 738, 992, 1062, 1085, 1207
PspPI GGNCC 1 cut(s) 1084
PsrI GAACNNNNNNTAC 2 cut(s) 1022, 1054
PstI CTGCAG 2 cut(s) 588, 700
PsuI RGATCY 1 cut(s) 990
RsaI GTAC 2 cut(s) 519, 1165
RsaNI GTAC 2 cut(s) 518, 1164
RseI CAYNNNNRTG 1 cut(s) 918
SalI GTCGAC 1 cut(s) 494
SaqAI TTAA 8 cut(s) 14, 113, 123, 164, 192, 213, 566, 747
SatI GCNGC 5 cut(s) 37, 76, 587, 590, 1105
Sau3AI GATC 7 cut(s) 223, 234, 477, 715, 730, 990, 1127
Sau96I GGNCC 1 cut(s) 1084
SchI GAGTC 1 cut(s) 1109
ScrFI CCNGG 3 cut(s) 344, 831, 865
SduI GDGCHC 1 cut(s) 1292
SfaNI GCATC 3 cut(s) 335, 457, 811
SfcI CTRYAG 2 cut(s) 584, 696
SfuI TTCGAA 1 cut(s) 616
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 1 cut(s) 918
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SpeI ACTAGT 1 cut(s) 97
SsiI CCGC 5 cut(s) 357, 501, 848, 946, 1105
SspI AATATT 2 cut(s) 670, 795
SspMI CTAG 2 cut(s) 98, 1233
StyD4I CCNGG 3 cut(s) 342, 829, 863
StyI CCWWGG 1 cut(s) 895
TaaI ACNGT 5 cut(s) 309, 517, 772, 778, 1150
TaiI ACGT 2 cut(s) 537, 714
TaqI TCGA 8 cut(s) 219, 273, 465, 495, 616, 1072, 1140, 1152
TauI GCSGC 1 cut(s) 1107
Tru1I TTAA 8 cut(s) 14, 113, 123, 164, 192, 213, 566, 747
Tru9I TTAA 8 cut(s) 14, 113, 123, 164, 192, 213, 566, 747
TseFI GTSAC 2 cut(s) 799, 905
TseI GCWGC 4 cut(s) 36, 75, 586, 589
Tsp45I GTSAC 2 cut(s) 799, 905
VpaK11BI GGWCC 1 cut(s) 1084
VspI ATTAAT 2 cut(s) 14, 192
XapI RAATTY 3 cut(s) 573, 1094, 1169
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 2 cut(s) 98, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.