AT3G46220

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007320
Physical Location & Seq
Forward (+)
16979048 .. 16984177
5130 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G46220.3

Sequence Viewer

Length: 2415 bp
ATGGATGATGAATTGTTGGAATTGCAGCGTCAGTTCGAGTTCGCTCAACAGGTGAAGTCAAGCGTGAGATTGTCGGATCGAAATGTTGTCGAATTGGTTCAGAAGCTTCAGGAGCTTGGCGTTATTGACTTCGATCTGCTTCATACCGTCACCGGAAAAGAATACATCACTCAGGAGCAATTGAGGAATGAAATTACTCGTGAGATCAGTAAATTGGGACGTGTCTCTGTTATTGATCTGGCAGATACGATTGGGGTTGATTTGTATCATGTGGAGAAGCAAGCACAAGATGTGGTGTTGAATGATCCAGGGTTAATGTTAGTTCAGGGAGAAATAATATCACAAAGTTATTGGGATTCGATTGCAGAAGAAATTAATGAGAGGCTTCAAGAGTGTAGCCAAATTGCTGTGGCTGAACTTGCTGGTCAGTTACAGGTTGGCTCTGAGTTGGTGCAATCGGTTTTAGAGCCTCGACTTGGAACTTTGGTGAAAGCTAGGCTAGAAGGTGGACAGTTATATACACCTGCTTATGTAGAACGTGTGACCGCTATGGTTCGAGGTGCTTCCAGGGGTATATTTGTTCCTTCCAATTTGTCGGCATTGTGGGCACCATTGCAACAGTTGGTACAAGAAACGAATGGAGCCAGTGGAGTTGCTGTTGAGAATTCATTTTTCCAGTCTATATTTAACCGACTGTTGAAGGAAGAGGAGATGCTTGGATCACTACGTGCTGGAACCCATTGGACTCCTAGTGCTTTTGCAACTGCTCAAAAGGAATGCGTAGACTCTTCTTTTTCACAGAATTCCTACATCTCCTATGAGAGCATGCAGAAGCTTGGGATTTCTCAAGCTGTGCAGTTCTTACAGTCTAGATACCCAGATGGCACACCTTTAGCTGCTGTATTTATTCATTCCTCAATGATTGAGATGCTGGATTCTGCAACTGAGGATGCTATTGAACAAAACAGTTGGATCGATTCCCTCTCTGTATTACCTTCATCGTTCACGTCACAGGATGCAAACAAGATGTTGCTTCTTTGTCCCTCTGTCCAATCAGCTCTCAAGGCTGAGAAAGCACTTATCTTGGGCGAATCCTATGTCCTAAGCAGTGGCTTTATCAAGGGAATATATGATCAAATTGAGAAAGAGGCAGATGCTTTTAGCATCCAAGCTTCTACTGCTACTTTGATACATCCTTCAAGCAAGTCGTCAGAGTCGACAGAAAGCATACCTGCCAATACAGATAAAGGGTCAAAGAAGAAAAAAGGAAAGTCTGCAAGCACGAAGGCAGCTACCGTTGAAACTGTTCCAGATGATGAAGAGGATGCCCGTCCAAAATCTAAGAGAAACCAGAAGAAAGGAAGGGATTCATCTTCATCACAGAAGTTGGATTCAAAAGCAGGAGGCAAGAAAGAGTCAGTTAAGGCGCAAGAAAGTAATAACATTATTCCGCCAGACGAATGGGTGATGAAAAAGATCGTCGACTCTGTACCTGAATTTGAAGATGATGGTACGGAGAACCCAGATTCGATTCTTAAGCATTTAGCTGATCATATGAAACCGATGCTCATTAATTCACTGAAGGAGAGAAGAAAGAAAATCTTCACAGAAAATGCAGATAGAATGAGGCGCTTGATTGATGATCTGCAGAAAAAGCTTGATGAGTCTTTCCTAAATATGCAGCTGTACGAAAAAGCGCTAGACCTTTTCGAAGATGACCAATCAACTGCAGTTGTTTTACATAGGCATCTGCTGAGAACAACAGCAGCTACAATTGCTGATACACTTCTTCATGGCTTGGACATCCACAACAAAATGAAGAATGGTACTGAAGTTGAAGAATCCAAAACGCAAGATCTAGTGTTGCTTGATTCTTCTGAGAGAACAGCCCTTGCTAAGAATCTCAACGGTTCACTTTCAAAAAAGGCTCTTGCACTAGTTGAGGCATTGGAAGGAAAGCGTGTAGACACTTTCATGGTCACCTTCAGAGACCTGGCAGAAGAAAGTGGGTTAGTCTTGAAGAAGCTTGACAAAAAACTGGAAAGGACACTCCTACATTCATATCGTAAGGATTTGATATCTCAAGTTTCCACCGAATCAGACCCTATTGCACTTCTCGCTAAAGTTGTCTCCCTCCTTTTTATTAAGATTCACAATAAAGCTTTACAAGCCCCTGGTAGAGCCATTGCTGCGGCTATTTCACACTTGAAGGAAAAACTTGATGAATCTGCGTACAAGACTCTGACAGATTACCAAACAGCAACTGTAACTCTTCTTGCTCTAATGTCAGCTTCATCCGGCGAGGAACATGATTGCTCCGCAGACAGAATCTTGACCAAACGGGAACTTCTCGAGAGCCAGATGCCATTGCTTAGAACCCTCGTCCTTGGTGACTCGCAGCCACAACAATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

804

Amino Acids

89.07

Weight (kDa)

5.28

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 4 - 271 3.9e-84 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 289 - 336 2.6e-08 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 531 - 660 1.2e-35 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 665 - 777 1e-30 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 532
Acc36I ACCTGC 2 cut(s) 532, 1240
AccB1I GGYRCC 1 cut(s) 607
AccI GTMKAC 4 cut(s) 783, 1217, 1482, 1965
AciI CCGC 4 cut(s) 546, 1451, 2192, 2319
AclWI GGATC 4 cut(s) 84, 299, 727, 980
AcsI RAATTY 3 cut(s) 664, 802, 1496
AcuI CTGAAG 4 cut(s) 92, 1601, 1851, 1969
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 6 cut(s) 627, 1491, 1513, 1688, 1828, 2234
AfeI AGCGCT 1 cut(s) 1698
AfiI CCNNNNNNNGG 1 cut(s) 476
AflII CTTAAG 1 cut(s) 1535
AflIII ACRYGT 2 cut(s) 220, 538
AhlI ACTAGT 1 cut(s) 1936
AjiI CACGTC 2 cut(s) 221, 1008
AjnI CCWGG 4 cut(s) 307, 566, 1992, 2173
Alw26I GTCTC 3 cut(s) 229, 1983, 2134
AlwI GGATC 4 cut(s) 84, 299, 727, 980
AlwNI CAGNNNCTG 1 cut(s) 2264
Ama87I CYCGRG 1 cut(s) 2351
Aor51HI AGCGCT 1 cut(s) 1698
ApeKI GCWGC 7 cut(s) 25, 896, 1289, 1681, 1766, 2189, 2398
ApoI RAATTY 3 cut(s) 664, 802, 1496
ArsI GACNNNNNNTTYG 2 cut(s) 1083, 1115
AseI ATTAAT 2 cut(s) 375, 1572
Asp700I GAANNNNTTC 4 cut(s) 96, 1305, 1366, 1601
AspLEI GCGC 3 cut(s) 1429, 1632, 1699
AsuHPI GGTGA 6 cut(s) 64, 142, 499, 1477, 1972, 2402
AsuII TTCGAA 1 cut(s) 1710
AvaI CYCGRG 1 cut(s) 2351
BaeGI GKGCMC 1 cut(s) 610
BaeI ACNNNNGTAYC 2 cut(s) 1818, 1851
BanI GGYRCC 1 cut(s) 607
BauI CACGAG 1 cut(s) 198
BbvI GCAGC 7 cut(s) 37, 883, 1301, 1693, 1778, 2176, 2410
BccI CCATC 2 cut(s) 875, 1502
BciT130I CCWGG 4 cut(s) 309, 568, 1994, 2175
BclI TGATCA 2 cut(s) 1132, 1549
BcoDI GTCTC 3 cut(s) 229, 1983, 2134
BcuI ACTAGT 1 cut(s) 1936
BfaI CTAG 7 cut(s) 495, 500, 750, 870, 1700, 1859, 1937
BfmI CTRYAG 2 cut(s) 1646, 1728
BfoI RGCGCY 2 cut(s) 1633, 1700
BfrI CTTAAG 1 cut(s) 1535
BfuAI ACCTGC 2 cut(s) 532, 1240
BglII AGATCT 1 cut(s) 1855
BisI GCNGC 8 cut(s) 26, 897, 1290, 1682, 1767, 2190, 2193, 2399
BlsI GCNGC 8 cut(s) 27, 898, 1291, 1683, 1768, 2191, 2194, 2400
Bme1390I CCNGG 4 cut(s) 309, 568, 1994, 2175
BmeT110I CYCGRG 1 cut(s) 2351
BmgBI CACGTC 2 cut(s) 221, 1008
BmiI GGNNCC 3 cut(s) 609, 643, 736
BmrFI CCNGG 4 cut(s) 309, 568, 1994, 2175
Bpu10I CCTNAGC 1 cut(s) 1103
Bpu14I TTCGAA 1 cut(s) 1710
BpuEI CTTGAG 3 cut(s) 831, 1046, 2067
Bsa29I ATCGAT 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 1983
BsaJI CCNNGG 4 cut(s) 308, 567, 2173, 2386
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse1I ACTGG 3 cut(s) 645, 676, 2043
Bse3DI GCAATG 3 cut(s) 611, 2184, 2366
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 4 cut(s) 309, 568, 1994, 2175
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 4 cut(s) 308, 567, 2173, 2386
BseGI GGATG 8 cut(s) 10, 955, 1021, 1164, 1192, 1330, 1803, 2294
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 3 cut(s) 611, 2184, 2366
BseMII CTCAG 6 cut(s) 185, 435, 936, 1059, 1745, 1869
BseNI ACTGG 3 cut(s) 645, 676, 2043
BseRI GAGGAG 1 cut(s) 722
BseSI GKGCMC 1 cut(s) 610
BseXI GCAGC 7 cut(s) 37, 883, 1301, 1693, 1778, 2176, 2410
BsgI GTGCAG 1 cut(s) 875
BshNI GGYRCC 1 cut(s) 607
BshVI ATCGAT 1 cut(s) 975
BsiHKCI CYCGRG 1 cut(s) 2351
BsiSI CCGG 2 cut(s) 153, 2298
BslFI GGGAC 2 cut(s) 231, 1026
BslI CCNNNNNNNGG 1 cut(s) 476
BsmAI GTCTC 3 cut(s) 229, 1983, 2134
BsmFI GGGAC 2 cut(s) 231, 1026
BsmI GAATGC 1 cut(s) 782
Bso31I GGTCTC 1 cut(s) 1983
BsoBI CYCGRG 1 cut(s) 2351
Bsp119I TTCGAA 1 cut(s) 1710
Bsp1286I GDGCHC 1 cut(s) 610
BspACI CCGC 4 cut(s) 546, 1451, 2192, 2319
BspCNI CTCAG 6 cut(s) 184, 436, 937, 1060, 1746, 1870
BspDI ATCGAT 1 cut(s) 975
BspLI GGNNCC 3 cut(s) 609, 643, 736
BspMAI CTGCAG 2 cut(s) 1650, 1732
BspMI ACCTGC 2 cut(s) 532, 1240
BspPI GGATC 4 cut(s) 84, 299, 727, 980
BspT104I TTCGAA 1 cut(s) 1710
BspT107I GGYRCC 1 cut(s) 607
BspTI CTTAAG 1 cut(s) 1535
BspTNI GGTCTC 1 cut(s) 1983
BsrDI GCAATG 3 cut(s) 611, 2184, 2366
BsrI ACTGG 3 cut(s) 645, 676, 2043
BssECI CCNNGG 4 cut(s) 308, 567, 2173, 2386
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 2386
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 4 cut(s) 309, 568, 1994, 2175
Bst6I CTCTTC 4 cut(s) 699, 793, 1314, 2277
BstAFI CTTAAG 1 cut(s) 1535
BstBAI YACGTR 1 cut(s) 728
BstBI TTCGAA 1 cut(s) 1710
BstC8I GCNNGC 3 cut(s) 282, 827, 1279
BstEII GGTNACC 1 cut(s) 1978
BstF5I GGATG 8 cut(s) 10, 955, 1021, 1164, 1192, 1330, 1803, 2294
BstH2I RGCGCY 2 cut(s) 1633, 1700
BstHHI GCGC 3 cut(s) 1429, 1632, 1699
BstMAI GTCTC 3 cut(s) 229, 1983, 2134
BstNI CCWGG 4 cut(s) 309, 568, 1994, 2175
BstNSI RCATGY 1 cut(s) 829
BstPI GGTNACC 1 cut(s) 1978
BstSCI CCNGG 4 cut(s) 307, 566, 1992, 2173
BstSFI CTRYAG 2 cut(s) 1646, 1728
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 7 cut(s) 37, 883, 1301, 1693, 1778, 2176, 2410
BstX2I RGATCY 1 cut(s) 1855
BstXI CCANNNNNNTGG 1 cut(s) 1461
BstYI RGATCY 1 cut(s) 1855
Bsu15I ATCGAT 1 cut(s) 975
BsuTUI ATCGAT 1 cut(s) 975
BtrI CACGTC 2 cut(s) 221, 1008
BtsCI GGATG 8 cut(s) 10, 955, 1021, 1164, 1192, 1330, 1803, 2294
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 3 cut(s) 652, 1114, 1577
BveI ACCTGC 2 cut(s) 532, 1240
Cac8I GCNNGC 3 cut(s) 282, 827, 1279
CaiI CAGNNNCTG 1 cut(s) 2264
CfoI GCGC 3 cut(s) 1429, 1632, 1699
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 17
Csp6I GTAC 6 cut(s) 626, 1490, 1512, 1687, 1827, 2233
CviAII CATG 5 cut(s) 269, 826, 1793, 1975, 2309
CviQI GTAC 6 cut(s) 626, 1490, 1512, 1687, 1827, 2233
DraIII CACNNNGTG 1 cut(s) 728
Eam1104I CTCTTC 4 cut(s) 699, 793, 1314, 2277
EarI CTCTTC 4 cut(s) 699, 793, 1314, 2277
EciI GGCGGA 1 cut(s) 1440
Eco130I CCWWGG 1 cut(s) 2386
Eco31I GGTCTC 1 cut(s) 1983
Eco32I GATATC 1 cut(s) 2079
Eco47III AGCGCT 1 cut(s) 1698
Eco57I CTGAAG 4 cut(s) 92, 1601, 1851, 1969
Eco88I CYCGRG 1 cut(s) 2351
Eco91I GGTNACC 1 cut(s) 1978
EcoO65I GGTNACC 1 cut(s) 1978
EcoRI GAATTC 2 cut(s) 664, 802
EcoRII CCWGG 4 cut(s) 307, 566, 1992, 2173
EcoRV GATATC 1 cut(s) 2079
EcoT14I CCWWGG 1 cut(s) 2386
ErhI CCWWGG 1 cut(s) 2386
FaeI CATG 5 cut(s) 272, 829, 1796, 1978, 2312
FalI AAGNNNNNCTT 2 cut(s) 1586, 1618
FaqI GGGAC 2 cut(s) 231, 1026
FatI CATG 5 cut(s) 268, 825, 1792, 1974, 2308
FauNDI CATATG 1 cut(s) 1554
FbaI TGATCA 2 cut(s) 1132, 1549
FblI GTMKAC 4 cut(s) 783, 1217, 1482, 1965
Fnu4HI GCNGC 8 cut(s) 26, 897, 1290, 1682, 1767, 2190, 2193, 2399
FokI GGATG 8 cut(s) 17, 962, 1028, 1151, 1179, 1337, 1790, 2281
Fsp4HI GCNGC 8 cut(s) 26, 897, 1290, 1682, 1767, 2190, 2193, 2399
FspBI CTAG 7 cut(s) 495, 500, 750, 870, 1700, 1859, 1937
GlaI GCGC 3 cut(s) 1428, 1631, 1698
GluI GCNGC 8 cut(s) 26, 897, 1290, 1682, 1767, 2190, 2193, 2399
HaeII RGCGCY 2 cut(s) 1633, 1700
HapII CCGG 2 cut(s) 153, 2298
HgaI GACGC 1 cut(s) 17
HhaI GCGC 3 cut(s) 1429, 1632, 1699
Hin1II CATG 5 cut(s) 272, 829, 1796, 1978, 2312
Hin6I GCGC 3 cut(s) 1427, 1630, 1697
HinP1I GCGC 3 cut(s) 1427, 1630, 1697
HincII GTYRAC 2 cut(s) 1218, 1483
HindII GTYRAC 2 cut(s) 1218, 1483
HindIII AAGCTT 6 cut(s) 104, 833, 1170, 1655, 2024, 2160
HpaII CCGG 2 cut(s) 153, 2298
HphI GGTGA 6 cut(s) 64, 142, 499, 1477, 1972, 2402
Hpy166II GTNNAC 7 cut(s) 509, 784, 1005, 1218, 1483, 1913, 1966
Hpy188I TCNGA 8 cut(s) 76, 102, 445, 1213, 1879, 1988, 2101, 2244
Hpy8I GTNNAC 7 cut(s) 509, 784, 1005, 1218, 1483, 1913, 1966
Hpy99I CGWCG 1 cut(s) 1484
HpyCH4IV ACGT 4 cut(s) 220, 538, 727, 1007
HpySE526I ACGT 4 cut(s) 220, 538, 727, 1007
Hsp92II CATG 5 cut(s) 272, 829, 1796, 1978, 2312
HspAI GCGC 3 cut(s) 1427, 1630, 1697
Ksp22I TGATCA 2 cut(s) 1132, 1549
LmnI GCTCC 4 cut(s) 112, 175, 641, 2321
Lsp1109I GCAGC 7 cut(s) 37, 883, 1301, 1693, 1778, 2176, 2410
MaeI CTAG 7 cut(s) 495, 500, 750, 870, 1700, 1859, 1937
MaeII ACGT 4 cut(s) 220, 538, 727, 1007
MaeIII GTNAC 7 cut(s) 148, 429, 541, 1008, 1978, 2266, 2390
MfeI CAATTG 2 cut(s) 179, 1773
MflI RGATCY 1 cut(s) 1855
MhlI GDGCHC 1 cut(s) 610
MlyI GAGTC 8 cut(s) 739, 779, 1223, 1424, 1478, 1673, 2233, 2387
MmeI TCCRAC 3 cut(s) 54, 950, 1368
MroXI GAANNNNTTC 4 cut(s) 96, 1305, 1366, 1601
MseI TTAA 7 cut(s) 314, 375, 687, 1422, 1536, 1572, 2145
MslI CAYNNNNRTG 1 cut(s) 1973
MspA1I CMGCKG 1 cut(s) 1684
MspCI CTTAAG 1 cut(s) 1535
MspI CCGG 2 cut(s) 153, 2298
MspR9I CCNGG 4 cut(s) 309, 568, 1994, 2175
MunI CAATTG 2 cut(s) 179, 1773
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 4 cut(s) 309, 568, 1994, 2175
NdeI CATATG 1 cut(s) 1554
NlaIII CATG 5 cut(s) 272, 829, 1796, 1978, 2312
NlaIV GGNNCC 3 cut(s) 609, 643, 736
NmuCI GTSAC 5 cut(s) 148, 541, 1008, 1978, 2390
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1710
PaeI GCATGC 1 cut(s) 829
PaeR7I CTCGAG 1 cut(s) 2351
PaqCI CACCTGC 1 cut(s) 532
PcsI WCGNNNNNNNCGW 1 cut(s) 1214
PctI GAATGC 1 cut(s) 782
PdmI GAANNNNTTC 4 cut(s) 96, 1305, 1366, 1601
PkrI GCNGC 8 cut(s) 27, 898, 1291, 1683, 1768, 2191, 2194, 2400
PleI GAGTC 8 cut(s) 739, 779, 1222, 1423, 1478, 1672, 2233, 2387
PpsI GAGTC 8 cut(s) 739, 779, 1222, 1423, 1478, 1672, 2233, 2387
Ppu21I YACGTR 1 cut(s) 728
PshBI ATTAAT 2 cut(s) 375, 1572
Psp6I CCWGG 4 cut(s) 307, 566, 1992, 2173
PspEI GGTNACC 1 cut(s) 1978
PspGI CCWGG 4 cut(s) 307, 566, 1992, 2173
PspN4I GGNNCC 3 cut(s) 609, 643, 736
PstI CTGCAG 2 cut(s) 1650, 1732
PstNI CAGNNNCTG 1 cut(s) 2264
PsuI RGATCY 1 cut(s) 1855
PvuII CAGCTG 1 cut(s) 1684
RsaI GTAC 6 cut(s) 627, 1491, 1513, 1688, 1828, 2234
RsaNI GTAC 6 cut(s) 626, 1490, 1512, 1687, 1827, 2233
RseI CAYNNNNRTG 1 cut(s) 1973
SalI GTCGAC 2 cut(s) 1216, 1481
SaqAI TTAA 7 cut(s) 314, 375, 687, 1422, 1536, 1572, 2145
SatI GCNGC 8 cut(s) 26, 897, 1290, 1682, 1767, 2190, 2193, 2399
SchI GAGTC 8 cut(s) 739, 779, 1223, 1424, 1478, 1673, 2233, 2387
ScrFI CCNGG 4 cut(s) 309, 568, 1994, 2175
SduI GDGCHC 1 cut(s) 610
SfcI CTRYAG 2 cut(s) 1646, 1728
Sfr274I CTCGAG 1 cut(s) 2351
SfuI TTCGAA 1 cut(s) 1710
SlaI CTCGAG 1 cut(s) 2351
SmiMI CAYNNNNRTG 1 cut(s) 1973
SmlI CTYRAG 5 cut(s) 846, 1061, 1535, 2082, 2351
SmoI CTYRAG 5 cut(s) 846, 1061, 1535, 2082, 2351
SpeI ACTAGT 1 cut(s) 1936
SphI GCATGC 1 cut(s) 829
SsiI CCGC 4 cut(s) 546, 1451, 2192, 2319
SspMI CTAG 7 cut(s) 495, 500, 750, 870, 1700, 1859, 1937
StyD4I CCNGG 4 cut(s) 307, 566, 1992, 2173
StyI CCWWGG 1 cut(s) 2386
TaiI ACGT 4 cut(s) 223, 541, 730, 1010
TauI GCSGC 1 cut(s) 2195
Tru1I TTAA 7 cut(s) 314, 375, 687, 1422, 1536, 1572, 2145
Tru9I TTAA 7 cut(s) 314, 375, 687, 1422, 1536, 1572, 2145
TscAI CASTG 3 cut(s) 652, 1114, 1584
TseFI GTSAC 5 cut(s) 148, 541, 1008, 1978, 2390
TseI GCWGC 7 cut(s) 25, 896, 1289, 1681, 1766, 2189, 2398
Tsp45I GTSAC 5 cut(s) 148, 541, 1008, 1978, 2390
TspGWI ACGGA 1 cut(s) 1529
TspRI CASTG 3 cut(s) 652, 1114, 1584
Vha464I CTTAAG 1 cut(s) 1535
VspI ATTAAT 2 cut(s) 375, 1572
XapI RAATTY 3 cut(s) 664, 802, 1496
XbaI TCTAGA 1 cut(s) 869
XceI RCATGY 1 cut(s) 829
XhoI CTCGAG 1 cut(s) 2351
XmiI GTMKAC 4 cut(s) 783, 1217, 1482, 1965
XmnI GAANNNNTTC 4 cut(s) 96, 1305, 1366, 1601
XspI CTAG 7 cut(s) 495, 500, 750, 870, 1700, 1859, 1937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.