FvH4_3g34630

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007320
Physical Location & Seq
Reverse (-)
30002146 .. 30010449
8304 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34630.t4

Sequence Viewer

Length: 2490 bp
ATGGACGACGAATTGCTGGAACTGCAGAAGCAATTCGAGTCGGCGCAGCAAGCGAAATCGAGCATCAGATTGTCCGATCGAAACGTCGTCGAGTTGGTCCAGAAGCTTCACGAGCTCCACATCATCGACTTCGAGCTTCTTCACACCGTTTCCGGCAAAGAGTACATCACTCCGGATCAATTGAGGCATGAAATACTGGTGGAGGTCAAGAGGTTGGGCCGGATATCGCTGATTGACCTTGCAGACACTATTGGGGTTGATTTGTACCATGTCGAGAAGCAATCTCAACATGTGGTGTCGGATGATCCGGGGCTTATGTTGATTCAAGGGGAAATAATAGCACAGTCGTATTGGGATTCTGTCGCTGAAGAAATCAATGAGAGGCTTCAAGAATGTAGCCAAGTTGCCTTGGCGGAACTTGCCGTGCAGTTGCATGTCAGCTCGGAGATGGTGACATCTGTGTTGGAGCCGCGCATTGGGACTATTGTGAAAGGTAGGCTTGAAGGCGGGCAGTTGTATACCCCTGCTTATGTTACGCGTGTTACTGCCATGGTACGTGGTGCTGCTAGGGCCATCACAGTTCCCACAAATTTATCAGTCTTGTGGAGTACTTTACAGCAGTTGCTGCAAGAAATGGAGGGAGCCAGTGGTGTAGCTGTAGAAGGTTCATTTTTCCAATCCCTTTTTAATGGGCTTGTAAAGGAAGGCGAGATCCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCAAATGTCTTTGCCATTGCCCAGAAGGAAACAATTGATTCTTTCTTCTCACAGAACTCTTTCATTGGCTATGATGTTCTGCAGAAACTTAGAATTCCTCAGCCTGTTCAGTTCTTACAGTCCAGATATCCTGAATGCATACCTTTGGTTACTACATTTATTCACCCATCAATGATTGAGATGCTTGATGCTGCTATAGAAGATGCTCTTGAGCGTGGGAGCTGGATGGATTCTCTTTCTATACTGCCAATGTCCTTTGGATCTCAAGATGCATCCAAACTTTTGTCTCTTTGTCCTTCCATTCAGCAGGGCCTCAAGGCTGATAAGGCAATAATCTTTGGGGAGTCGTTCGTATTTTGTAGAGCCTTTATCAAGGACGTGTATGATCGCCTGGAGAAGGAAATGGAAACTCTTATAGTTTCGAATTCTTCTGGTACTGTGATGTCAGAAGACTTGCAGGGGACAAAAGTTGGTCATGACACAGGCAGGTTCACTGAATCAAATGAAACGACTAGTGACAGCAGCAGTAATAAGCAGACGATGGAGAAAGGATCAAAGAAGAAAAAGGGACGAGTGACTGGGAATATAGGAGCAGGTGTGGCCGAGAGTGATCCGGATAACCAGGACTCTGTTCCAACAAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATTCCTCCTCCGCACAAGTTGCTGACTCGAAAGCATCTGCTAAGTTGGTAAAATCTAAAGAGGAAAATCTGAACATTCCTTCAGAAGATTGGATGGTGAACAAGATTGCAACACTGGTTCCTGATTTTGAAGAACAAGAGCAATGGTCAAATTTGATAGGTCTTGATGATCCACAAACAATAATTAGACCTCTTGCAAACTATATGAGACCTATGCTAATAAACTCTTGGAAGGAGAGAAGAAAGGCATTGTTTACTGAAAATGCAGAGAGAATGAAGCACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAACATGCAGCTCTATGAAAAAGCTTTAGAATTGTTTGAAGATGACCAATCAACTTCAGTCATTTTGCACCGGCATTTGTTAAGAACAACAGCTACTACTATTGTGGATATGCTTCTTCATAATTTGGACATGCACAATAAATTGAAGAATGGAGTTGAAGTTGAGGACACTCAGATTTCAGAATCTTCACTTAATCCAGGAGAAAGAACTTCAATTGCTAAGAATTTTCCTGGATCTCTCTCAAAGAAGGCTCTTGTTGTTGTTGAAGCTTTGGAAGGAAAGCGGGTGGAAACATTCATGACTGCTTTAAGAGACATAGCTGAAGAAAGTGGTTTACTTCTGAAAAAGCTTGACAAGAAGTTGGAAAGAACACTTCTGCATTCGTATCAAAAGGATTTGGCGTCTCAAGTTTCTGCTGAAATGGATCCAATCTCTATTTTAGCCAAAGTTGTTTCCCTGCTTTATGTACAGATTCACCATAAAGCTCTCCAAGCACCTGGAAGGGCCATCTCTGTTGCTGTATCTCGGCTGAAGGATAAACTAGATGAATCGGCGTTCAAGATCCTGACAGAATATCAAACTGCAACTGTAACCCTCTTAGCACTAATGTCCGCTGCAAGTGGAGAGGGAGAAGACTGTACATCTGATAGGATATTGAGCAAAAGGGAGCTCCTGGAAAATCAAATTCCTGCACTGAGAGGCCTGGTTTTGCGCACGGGCACCTCACAATCTACACTTGGCATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

830

Amino Acids

92.31

Weight (kDa)

5.48

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 1 - 183 5.8e-55 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 200 - 247 1.1e-09 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 456 - 585 1.6e-39 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 590 - 701 1.5e-28 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1331
Acc16I TGCGCA 1 cut(s) 2453
Acc36I ACCTGC 2 cut(s) 1224, 1331
AccB1I GGYRCC 1 cut(s) 2459
AccI GTMKAC 1 cut(s) 518
AccII CGCG 2 cut(s) 472, 538
AccIII TCCGGA 2 cut(s) 172, 1360
AciI CCGC 6 cut(s) 413, 470, 507, 1428, 2023, 2352
AcoI YGGCCR 1 cut(s) 1347
AcsI RAATTY 7 cut(s) 589, 840, 1171, 1417, 1567, 1963, 2424
AcuI CTGAAG 5 cut(s) 387, 1482, 1779, 2082, 2291
AcyI GRCGYC 1 cut(s) 2141
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 7 cut(s) 164, 266, 555, 610, 1183, 2208, 2380
AfiI CCNNNNNNNGG 2 cut(s) 476, 1144
AflIII ACRYGT 3 cut(s) 289, 536, 1125
AhlI ACTAGT 1 cut(s) 1259
AjiI CACGTC 1 cut(s) 1126
AjnI CCWGG 7 cut(s) 1137, 1368, 1936, 1969, 2236, 2412, 2442
Alw21I GWGCWC 2 cut(s) 117, 2412
Alw26I GTCTC 4 cut(s) 1038, 1618, 2046, 2148
AlwNI CAGNNNCTG 1 cut(s) 625
Aor13HI TCCGGA 2 cut(s) 172, 1360
AoxI GGCC 6 cut(s) 217, 570, 1057, 1347, 2244, 2440
ApeKI GCWGC 7 cut(s) 46, 563, 625, 938, 1269, 1747, 2354
ApoI RAATTY 7 cut(s) 589, 840, 1171, 1417, 1567, 1963, 2424
Asp700I GAANNNNTTC 2 cut(s) 32, 806
AspLEI GCGC 3 cut(s) 46, 474, 2454
AspS9I GGNCC 5 cut(s) 97, 217, 570, 1057, 2244
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 4 cut(s) 463, 902, 1525, 2207
AsuII TTCGAA 1 cut(s) 1169
AvaII GGWCC 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 2462
BamHI GGATCC 1 cut(s) 2164
BanI GGYRCC 1 cut(s) 2459
BanII GRGCYC 2 cut(s) 117, 2412
BauI CACGAG 1 cut(s) 110
BbsI GAAGAC 2 cut(s) 1203, 2379
Bbv12I GWGCWC 2 cut(s) 117, 2412
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 7 cut(s) 58, 550, 612, 925, 1281, 1759, 2341
BccI CCATC 7 cut(s) 442, 581, 922, 967, 1282, 1504, 2255
BceAI ACGGC 1 cut(s) 407
BciT130I CCWGG 7 cut(s) 1139, 1370, 1938, 1971, 2238, 2414, 2444
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 4 cut(s) 1038, 1618, 2046, 2148
BcuI ACTAGT 1 cut(s) 1259
BfaI CTAG 3 cut(s) 567, 1260, 2282
BfmI CTRYAG 4 cut(s) 23, 657, 827, 942
BfuAI ACCTGC 2 cut(s) 1224, 1331
BisI GCNGC 8 cut(s) 47, 470, 564, 626, 939, 1270, 1748, 2355
BlsI GCNGC 8 cut(s) 48, 471, 565, 627, 940, 1271, 1749, 2356
BmcAI AGTACT 1 cut(s) 610
Bme1390I CCNGG 8 cut(s) 309, 1139, 1370, 1938, 1971, 2238, 2414, 2444
Bme18I GGWCC 1 cut(s) 97
BmgBI CACGTC 1 cut(s) 1126
BmgT120I GGNCC 5 cut(s) 97, 217, 570, 1057, 2244
BmiI GGNNCC 5 cut(s) 468, 643, 1536, 2166, 2461
BmrFI CCNGG 8 cut(s) 309, 1139, 1370, 1938, 1971, 2238, 2414, 2444
BmrI ACTGGG 1 cut(s) 1335
BmsI GCATC 7 cut(s) 72, 918, 925, 940, 1006, 1028, 1460
BmuI ACTGGG 1 cut(s) 1335
BpiI GAAGAC 2 cut(s) 1203, 2379
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 1169
BpuEI CTTGAG 4 cut(s) 977, 996, 1046, 2130
BpuMI CCSGG 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 2141
BsaI GGTCTC 1 cut(s) 1618
BsaJI CCNNGG 4 cut(s) 308, 408, 549, 715
BsaWI WCCGGW 2 cut(s) 172, 1360
BsaXI ACNNNNNCTCC 4 cut(s) 1329, 1359, 1884, 1914
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1144
Bse118I RCCGGY 1 cut(s) 1809
Bse1I ACTGG 4 cut(s) 201, 645, 1330, 1536
Bse3DI GCAATG 2 cut(s) 762, 1565
BseAI TCCGGA 2 cut(s) 172, 1360
BseBI CCWGG 7 cut(s) 1139, 1370, 1938, 1971, 2238, 2414, 2444
BseDI CCNNGG 4 cut(s) 308, 408, 549, 715
BseGI GGATG 4 cut(s) 307, 978, 1019, 1515
BseLI CCNNNNNNNGG 2 cut(s) 476, 1144
BseMI GCAATG 2 cut(s) 762, 1565
BseMII CTCAG 3 cut(s) 860, 1925, 2426
BseNI ACTGG 4 cut(s) 201, 645, 1330, 1536
BseRI GAGGAG 1 cut(s) 1414
BseSI GKGCMC 1 cut(s) 2462
BseXI GCAGC 7 cut(s) 58, 550, 612, 925, 1281, 1759, 2341
BsgI GTGCAG 3 cut(s) 446, 750, 2415
Bsh1236I CGCG 2 cut(s) 472, 538
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 6 cut(s) 219, 572, 1059, 1349, 2246, 2442
BshNI GGYRCC 1 cut(s) 2459
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 117, 2412
BsiSI CCGG 6 cut(s) 153, 173, 220, 308, 1361, 1810
BslFI GGGAC 3 cut(s) 493, 1222, 1329
BslI CCNNNNNNNGG 2 cut(s) 476, 1144
BsmAI GTCTC 4 cut(s) 1038, 1618, 2046, 2148
BsmBI CGTCTC 1 cut(s) 2148
BsmFI GGGAC 3 cut(s) 493, 1222, 1329
BsmI GAATGC 2 cut(s) 887, 2119
BsnI GGCC 6 cut(s) 219, 572, 1059, 1349, 2246, 2442
Bso31I GGTCTC 1 cut(s) 1618
Bsp119I TTCGAA 1 cut(s) 1169
Bsp1286I GDGCHC 3 cut(s) 117, 2412, 2462
Bsp13I TCCGGA 2 cut(s) 172, 1360
Bsp1407I TGTACA 2 cut(s) 2206, 2378
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 6 cut(s) 413, 470, 507, 1428, 2023, 2352
BspANI GGCC 6 cut(s) 219, 572, 1059, 1349, 2246, 2442
BspCNI CTCAG 3 cut(s) 859, 1924, 2427
BspEI TCCGGA 2 cut(s) 172, 1360
BspFNI CGCG 2 cut(s) 472, 538
BspHI TCATGA 2 cut(s) 1222, 2037
BspLI GGNNCC 5 cut(s) 468, 643, 1536, 2166, 2461
BspMAI CTGCAG 2 cut(s) 27, 831
BspMI ACCTGC 2 cut(s) 1224, 1331
BspT104I TTCGAA 1 cut(s) 1169
BspT107I GGYRCC 1 cut(s) 2459
BspTNI GGTCTC 1 cut(s) 1618
BsrDI GCAATG 2 cut(s) 762, 1565
BsrFI RCCGGY 1 cut(s) 1809
BsrGI TGTACA 2 cut(s) 2206, 2378
BsrI ACTGG 4 cut(s) 201, 645, 1330, 1536
BssAI RCCGGY 1 cut(s) 1809
BssECI CCNNGG 4 cut(s) 308, 408, 549, 715
BssNAI GTATAC 1 cut(s) 519
BssNI GRCGYC 1 cut(s) 2141
BssSI CACGAG 1 cut(s) 110
BssT1I CCWWGG 3 cut(s) 408, 549, 715
Bst1107I GTATAC 1 cut(s) 519
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 7 cut(s) 1139, 1370, 1938, 1971, 2238, 2414, 2444
Bst4CI ACNGT 7 cut(s) 148, 345, 580, 867, 1186, 2329, 2378
BstACI GRCGYC 1 cut(s) 2141
BstAPI GCANNNNNTGC 2 cut(s) 625, 1436
BstAUI TGTACA 2 cut(s) 2206, 2378
BstBAI YACGTR 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 1169
BstC8I GCNNGC 2 cut(s) 51, 509
BstDEI CTNAG 8 cut(s) 836, 846, 1392, 1458, 1911, 1959, 2338, 2435
BstDSI CCRYGG 1 cut(s) 549
BstENI CCTNNNNNAGG 1 cut(s) 1142
BstF5I GGATG 4 cut(s) 307, 978, 1019, 1515
BstFNI CGCG 2 cut(s) 472, 538
BstHHI GCGC 3 cut(s) 46, 474, 2454
BstMAI GTCTC 4 cut(s) 1038, 1618, 2046, 2148
BstMCI CGRYCG 1 cut(s) 79
BstNI CCWGG 7 cut(s) 1139, 1370, 1938, 1971, 2238, 2414, 2444
BstNSI RCATGY 4 cut(s) 293, 437, 1747, 1873
BstSCI CCNGG 8 cut(s) 307, 1137, 1368, 1936, 1969, 2236, 2412, 2442
BstSFI CTRYAG 4 cut(s) 23, 657, 827, 942
BstSLI GKGCMC 1 cut(s) 2462
BstUI CGCG 2 cut(s) 472, 538
BstV1I GCAGC 7 cut(s) 58, 550, 612, 925, 1281, 1759, 2341
BstV2I GAAGAC 2 cut(s) 1203, 2379
BstX2I RGATCY 5 cut(s) 711, 1007, 1973, 2164, 2301
BstXI CCANNNNNNTGG 1 cut(s) 2237
BstYI RGATCY 5 cut(s) 711, 1007, 1973, 2164, 2301
BstZ17I GTATAC 1 cut(s) 519
BsuRI GGCC 6 cut(s) 219, 572, 1059, 1349, 2246, 2442
BtgI CCRYGG 1 cut(s) 549
BtrI CACGTC 1 cut(s) 1126
BtsCI GGATG 4 cut(s) 307, 978, 1019, 1515
BtsIMutI CAGTG 4 cut(s) 652, 1239, 1529, 2432
BveI ACCTGC 2 cut(s) 1224, 1331
Cac8I GCNNGC 2 cut(s) 51, 509
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 2 cut(s) 1222, 2037
CfoI GCGC 3 cut(s) 46, 474, 2454
Cfr10I RCCGGY 1 cut(s) 1809
Cfr13I GGNCC 5 cut(s) 97, 217, 570, 1057, 2244
CseI GACGC 1 cut(s) 2130
Csp6I GTAC 7 cut(s) 163, 265, 554, 609, 1182, 2207, 2379
CviQI GTAC 7 cut(s) 163, 265, 554, 609, 1182, 2207, 2379
DdeI CTNAG 8 cut(s) 836, 846, 1392, 1458, 1911, 1959, 2338, 2435
DraIII CACNNNGTG 1 cut(s) 728
EaeI YGGCCR 1 cut(s) 1347
EciI GGCGGA 1 cut(s) 428
Ecl136II GAGCTC 2 cut(s) 115, 2410
Eco130I CCWWGG 3 cut(s) 408, 549, 715
Eco147I AGGCCT 1 cut(s) 2442
Eco24I GRGCYC 2 cut(s) 117, 2412
Eco31I GGTCTC 1 cut(s) 1618
Eco32I GATATC 2 cut(s) 225, 875
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 2 cut(s) 115, 2410
Eco57I CTGAAG 5 cut(s) 387, 1482, 1779, 2082, 2291
EcoICRI GAGCTC 2 cut(s) 115, 2410
EcoNI CCTNNNNNAGG 1 cut(s) 1142
EcoO109I RGGNCCY 1 cut(s) 1057
EcoRI GAATTC 3 cut(s) 840, 1171, 1417
EcoRII CCWGG 7 cut(s) 1137, 1368, 1936, 1969, 2236, 2412, 2442
EcoRV GATATC 2 cut(s) 225, 875
EcoT14I CCWWGG 3 cut(s) 408, 549, 715
EcoT22I ATGCAT 2 cut(s) 887, 1021
EcoT38I GRGCYC 2 cut(s) 117, 2412
ErhI CCWWGG 3 cut(s) 408, 549, 715
Esp3I CGTCTC 1 cut(s) 2148
FalI AAGNNNNNCTT 4 cut(s) 483, 515, 939, 971
FaqI GGGAC 3 cut(s) 493, 1222, 1329
FauI CCCGC 2 cut(s) 500, 2016
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 8 cut(s) 47, 470, 564, 626, 939, 1270, 1748, 2355
FokI GGATG 4 cut(s) 314, 985, 1006, 1522
FriOI GRGCYC 2 cut(s) 117, 2412
Fsp4HI GCNGC 8 cut(s) 47, 470, 564, 626, 939, 1270, 1748, 2355
FspBI CTAG 3 cut(s) 567, 1260, 2282
FspI TGCGCA 1 cut(s) 2453
GlaI GCGC 3 cut(s) 45, 473, 2453
GluI GCNGC 8 cut(s) 47, 470, 564, 626, 939, 1270, 1748, 2355
GsuI CTGGAG 1 cut(s) 1160
HaeIII GGCC 6 cut(s) 219, 572, 1059, 1349, 2246, 2442
HapII CCGG 6 cut(s) 153, 173, 220, 308, 1361, 1810
HgaI GACGC 1 cut(s) 2130
HhaI GCGC 3 cut(s) 46, 474, 2454
Hin1I GRCGYC 1 cut(s) 2141
Hin6I GCGC 3 cut(s) 44, 472, 2452
HinP1I GCGC 3 cut(s) 44, 472, 2452
HindIII AAGCTT 4 cut(s) 104, 1761, 2007, 2087
HpaII CCGG 6 cut(s) 153, 173, 220, 308, 1361, 1810
HphI GGTGA 4 cut(s) 463, 902, 1525, 2207
Hpy166II GTNNAC 5 cut(s) 519, 1239, 1516, 1671, 2075
Hpy8I GTNNAC 5 cut(s) 519, 1239, 1516, 1671, 2075
Hpy99I CGWCG 3 cut(s) 11, 89, 92
HpyCH4III ACNGT 7 cut(s) 148, 345, 580, 867, 1186, 2329, 2378
HpyCH4IV ACGT 3 cut(s) 84, 556, 1125
HpyF3I CTNAG 8 cut(s) 836, 846, 1392, 1458, 1911, 1959, 2338, 2435
HpySE526I ACGT 3 cut(s) 84, 556, 1125
Hsp92I GRCGYC 1 cut(s) 2141
HspAI GCGC 3 cut(s) 44, 472, 2452
Kpn2I TCCGGA 2 cut(s) 172, 1360
LmnI GCTCC 7 cut(s) 120, 466, 641, 966, 1337, 2407, 2415
Lsp1109I GCAGC 7 cut(s) 58, 550, 612, 925, 1281, 1759, 2341
LweI GCATC 7 cut(s) 72, 918, 925, 940, 1006, 1028, 1460
MaeI CTAG 3 cut(s) 567, 1260, 2282
MaeII ACGT 3 cut(s) 84, 556, 1125
MaeIII GTNAC 7 cut(s) 451, 532, 541, 895, 1262, 1321, 2329
MfeI CAATTG 3 cut(s) 179, 780, 1953
MflI RGATCY 5 cut(s) 711, 1007, 1973, 2164, 2301
MhlI GDGCHC 3 cut(s) 117, 2412, 2462
MluI ACGCGT 1 cut(s) 536
MlyI GAGTC 5 cut(s) 47, 1100, 1367, 1436, 1739
MmeI TCCRAC 4 cut(s) 279, 444, 1406, 2082
Mph1103I ATGCAT 2 cut(s) 887, 1021
MroI TCCGGA 2 cut(s) 172, 1360
MroXI GAANNNNTTC 2 cut(s) 32, 806
MseI TTAA 5 cut(s) 687, 1739, 1820, 1932, 2048
MspA1I CMGCKG 1 cut(s) 2354
MspI CCGG 6 cut(s) 153, 173, 220, 308, 1361, 1810
MspR9I CCNGG 8 cut(s) 309, 1139, 1370, 1938, 1971, 2238, 2414, 2444
MunI CAATTG 3 cut(s) 179, 780, 1953
Mva1269I GAATGC 2 cut(s) 887, 2119
MvaI CCWGG 7 cut(s) 1139, 1370, 1938, 1971, 2238, 2414, 2444
MvnI CGCG 2 cut(s) 472, 538
NciI CCSGG 1 cut(s) 309
NcoI CCATGG 1 cut(s) 549
NlaIV GGNNCC 5 cut(s) 468, 643, 1536, 2166, 2461
NmeAIII GCCGAG 2 cut(s) 1375, 2245
NmuCI GTSAC 3 cut(s) 451, 1262, 1321
NsbI TGCGCA 1 cut(s) 2453
NsiI ATGCAT 2 cut(s) 887, 1021
NspI RCATGY 4 cut(s) 293, 437, 1747, 1873
NspV TTCGAA 1 cut(s) 1169
PagI TCATGA 2 cut(s) 1222, 2037
PaqCI CACCTGC 1 cut(s) 1331
PceI AGGCCT 1 cut(s) 2442
PciI ACATGT 1 cut(s) 289
PctI GAATGC 2 cut(s) 887, 2119
PdmI GAANNNNTTC 2 cut(s) 32, 806
PfeI GAWTC 8 cut(s) 322, 356, 785, 977, 1244, 1922, 2212, 2288
PfoI TCCNGGA 3 cut(s) 1936, 1969, 2412
PkrI GCNGC 8 cut(s) 48, 471, 565, 627, 940, 1271, 1749, 2356
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 5 cut(s) 46, 1099, 1367, 1436, 1738
PpsI GAGTC 5 cut(s) 46, 1099, 1367, 1436, 1738
Ppu21I YACGTR 1 cut(s) 557
PscI ACATGT 1 cut(s) 289
Psp124BI GAGCTC 2 cut(s) 117, 2412
Psp6I CCWGG 7 cut(s) 1137, 1368, 1936, 1969, 2236, 2412, 2442
PspGI CCWGG 7 cut(s) 1137, 1368, 1936, 1969, 2236, 2412, 2442
PspN4I GGNNCC 5 cut(s) 468, 643, 1536, 2166, 2461
PspPI GGNCC 5 cut(s) 97, 217, 570, 1057, 2244
PsrI GAACNNNNNNTAC 2 cut(s) 1816, 1848
PstI CTGCAG 2 cut(s) 27, 831
PstNI CAGNNNCTG 1 cut(s) 625
PsuI RGATCY 5 cut(s) 711, 1007, 1973, 2164, 2301
PvuI CGATCG 1 cut(s) 79
RsaI GTAC 7 cut(s) 164, 266, 555, 610, 1183, 2208, 2380
RsaNI GTAC 7 cut(s) 163, 265, 554, 609, 1182, 2207, 2379
SacI GAGCTC 2 cut(s) 117, 2412
SaqAI TTAA 5 cut(s) 687, 1739, 1820, 1932, 2048
SatI GCNGC 8 cut(s) 47, 470, 564, 626, 939, 1270, 1748, 2355
Sau96I GGNCC 5 cut(s) 97, 217, 570, 1057, 2244
ScaI AGTACT 1 cut(s) 610
SchI GAGTC 5 cut(s) 47, 1100, 1367, 1436, 1739
ScrFI CCNGG 8 cut(s) 309, 1139, 1370, 1938, 1971, 2238, 2414, 2444
SduI GDGCHC 3 cut(s) 117, 2412, 2462
SfaNI GCATC 7 cut(s) 72, 918, 925, 940, 1006, 1028, 1460
SfcI CTRYAG 4 cut(s) 23, 657, 827, 942
SfuI TTCGAA 1 cut(s) 1169
SinI GGWCC 1 cut(s) 97
SmlI CTYRAG 4 cut(s) 956, 1011, 1061, 2145
SmoI CTYRAG 4 cut(s) 956, 1011, 1061, 2145
SpeI ACTAGT 1 cut(s) 1259
SseBI AGGCCT 1 cut(s) 2442
SsiI CCGC 6 cut(s) 413, 470, 507, 1428, 2023, 2352
SspMI CTAG 3 cut(s) 567, 1260, 2282
SstI GAGCTC 2 cut(s) 117, 2412
StuI AGGCCT 1 cut(s) 2442
StyD4I CCNGG 8 cut(s) 307, 1137, 1368, 1936, 1969, 2236, 2412, 2442
StyI CCWWGG 3 cut(s) 408, 549, 715
TaaI ACNGT 7 cut(s) 148, 345, 580, 867, 1186, 2329, 2378
TaiI ACGT 3 cut(s) 87, 559, 1128
TaqI TCGA 9 cut(s) 36, 59, 79, 90, 126, 132, 273, 1169, 1445
TatI WGTACW 4 cut(s) 162, 608, 2206, 2378
TauI GCSGC 1 cut(s) 472
TfiI GAWTC 8 cut(s) 322, 356, 785, 977, 1244, 1922, 2212, 2288
Tru1I TTAA 5 cut(s) 687, 1739, 1820, 1932, 2048
Tru9I TTAA 5 cut(s) 687, 1739, 1820, 1932, 2048
TscAI CASTG 4 cut(s) 652, 1246, 1536, 2439
TseFI GTSAC 3 cut(s) 451, 1262, 1321
TseI GCWGC 7 cut(s) 46, 563, 625, 938, 1269, 1747, 2354
Tsp45I GTSAC 3 cut(s) 451, 1262, 1321
TspRI CASTG 4 cut(s) 652, 1246, 1536, 2439
VpaK11BI GGWCC 1 cut(s) 97
XagI CCTNNNNNAGG 1 cut(s) 1142
XapI RAATTY 7 cut(s) 589, 840, 1171, 1417, 1567, 1963, 2424
XceI RCATGY 4 cut(s) 293, 437, 1747, 1873
XmiI GTMKAC 1 cut(s) 518
XmnI GAANNNNTTC 2 cut(s) 32, 806
XspI CTAG 3 cut(s) 567, 1260, 2282
ZrmI AGTACT 1 cut(s) 610
Zsp2I ATGCAT 2 cut(s) 887, 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.