MD03G1103200.v1.1

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007320
Physical Location & Seq
Forward (+)
8821168 .. 8829007
7840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1103200.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGGACGACGAATTGCTAGAATTGCAGAGGCAATTCGAGTTCGCGCAGCAAGCGAAATCGAGCATCAGATTATCCGATCGAAACGTCGTCGAATTGGTCCAGAAGCTTCAGGAGCTCCACATCATCGACTTCGAGCTCCTCCACACCGTCACCGGCAAAGAATACATTACTCCCGATCAATTGAGGAGCGAAATATCGGCGGAGGTCAGTAAACTAGGGCGTGTTTCGTTGATTGACCTTGCGGACACTACCGGAGTGGATTTGTATCATGTTGAGAAGCAAGCTCAGCATGTTGTTTTGGATGATCCGGGGCTTATGTTGATTCAAGGCGAAATAATATCACAGTCTTATTGGGATTCTGTCGCCGAAGAAGTCAATGACAGGCTTCAAGAGTGTAGCCAAATCGCTCTGGCAGAACTTGCCGCGCAGTTGCATGTCAGTTCGGAAATGGTGGCATCTGTGTTGGAGCCCCGCCTTGGGACTTTGGTGAAAGGTAGGCTTGAAGGTGGGCAGTTGTATACTCCTGCATATGTTGCACGGGTTACTGCCATGGTTCGTGGTGCTGCCAGAGGTATCGCAGTTCCCACAAACTTATCATTATTGTGGAGTTCGTTACAGCAGCTGTTGCAAGAAATGGATGGAGCCAGTGGAGTGGCTGTAGAAGGTTCATTTTTCCAGTCCCTGTTTAATGGGCTTATAAAGGAAGGCGAAATTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCTAATGTCTTTGCCATTGCTCAAAAGGAATCAATTGATTCTTTCTTTTCACAGAATTCTTTCATTAACTATGATGTTCTGCACAAACTTAGAATTCCTCAGCCCATTCAGTTCTTGCAGTCCAGATATCCAGAAGGCATACCTTTGGTTACTACATTTGTTCACCCCTCAATGATTGAGATGCTTGATGCTGCTACAGAAGATGCTCTCGAACGTGATAGCTGGATCGATTCTCTTTCTATACTGCCGATGTCCTTTGGGTCTCAGGATGCATCTAAACTTTTGTCCCTTTGCCCTTCGATTCAGCAGGGTCTCAAGTCTGACAAAGCAATAATCTTCGGGGAGTCATATGTATTTAGCAGCGGCTTTATCAAGGATGTGTATGATCGCCTGGAGAAAGAAATGGAAACCTTTAGTGTTTCAGTTCCTTCCAGTACTGTGGTGTCAGATGATTTGCAGGAGACAAAAGTTGGTCATGACACAAGCAGGTCGACTGAGTCCAATGAAACCGTTAGTGACAGCAGCAGTAATAAACAGGCAGCGGAGAAGGGATCAAAAAAGAAAAAAGGTAGAGGGGCTGGAAGCATGATAACAGGGCCAGCTGAGAGTGAGCTGGACAACCAGGACAATGTTCCTACAAGATCTAAGAAAAACCAAAGGAAAGGCAAGAATATCTCTTCCGGACAGGCCGCAGAGTCAAAAGCAGCTGCTAAGTTGGTAAAAATAAAAGAGGAAAATCTCAATGTCCCTTCAGAAGATTGGGTAATGAATAAGATTACAGCGCTTGTATCTGATTTTGAAGAACAAGGTCCTGATGATCCCCAAACAATTCTTGGACCTTTGGCACACTATTTGAGGCCTAAGTTAATCAACTCCTGGAAGGAGAGAAGAAAGGCATTGTTTACAGAAAATGTGGAGAGAATGAGGAACTTACTTGATAACTTGCAAAAGAAACTTGATGAGTCTTTCTTAAATATGCAGCTTTATGAAAAGGCTTTAGATTTGTTTGAAGATGACCAATCAACCTCAGTCATTTTTCACCGGCATCTGTTAAGAACAACAGCTACTACCATTGCCGATATGCTTCTTCAAAATTTGGACATGCACAACAAACTGAAGAATGGAGTTGAAGTTGCAGAGCCTCAAATTTCAGAATCTATTACCCTCAACACGGGAGAGAGAACTTCCATTGCCAAAAACTTTCCTGGATCGCTTTCAAGCAAGGCTCTTGCTGTAGTTGAAGCATTGGAAGGAAAGCGGGTGGAAACTTTCATGACTGCTCTAAGAGATATTGCAGAAGAGAGCGGCTTGCTCTTGAGAAAGATTGACAAGAAATTGGAAAGAACGCTTCTGCATTCGTATCAGAAGGATTTGGTGTCTCAAGTTTCTGCTGAGACAGATCCAGTTCCTCTTTTGCCAAAAGTTGTTTCCCTGATTTACGTACAGGTTCACCATAAAGCTCTTCAAGCACCTGGAAGGGCCATCGCTGTCGCTGTTTCACGACTGAAGGATAAACTAGATGAATCAGCCTTCAAGATCCTGACAGATTATCAAACTGCAACAGTGACCCTGTTAACGTTAATATCTGCTGCAAGTGGAGACGAAGAAGACTGTACATCGGATAGAATTTTGAGCAAAAGGGAGCTTTTGGAGACCCAAATGCCCGCACTCAAAGGCCTGGTCTTGCGCAACTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

817

Amino Acids

90.45

Weight (kDa)

5.23

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 4 - 288 7.7e-98 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 305 - 352 1.2e-07 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 561 - 690 1.1e-38 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 696 - 809 2.1e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 698
Acc16I TGCGCA 1 cut(s) 2435
Acc36I ACCTGC 1 cut(s) 1224
AccBSI CCGCTC 1 cut(s) 2052
AccI GTMKAC 2 cut(s) 518, 1238
AccII CGCG 2 cut(s) 44, 425
AccIII TCCGGA 1 cut(s) 1427
AclI AACGTT 1 cut(s) 2324
AclWI GGATC 8 cut(s) 299, 727, 980, 1306, 1559, 1963, 2141, 2278
AcsI RAATTY 6 cut(s) 711, 802, 840, 1840, 1893, 2373
AcuI CTGAAG 4 cut(s) 92, 1482, 1883, 2273
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 3 cut(s) 1183, 2190, 2362
AfeI AGCGCT 1 cut(s) 1530
AfiI CCNNNNNNNGG 3 cut(s) 476, 477, 1918
AjnI CCWGG 6 cut(s) 1137, 1368, 1622, 1951, 2218, 2424
AjuI GAANNNNNNNTTGG 2 cut(s) 2158, 2190
Alw21I GWGCWC 2 cut(s) 117, 138
Alw26I GTCTC 7 cut(s) 1014, 1064, 1202, 2130, 2135, 2340, 2393
AlwI GGATC 8 cut(s) 299, 727, 980, 1306, 1559, 1963, 2141, 2278
AlwNI CAGNNNCTG 2 cut(s) 622, 682
Aor13HI TCCGGA 1 cut(s) 1427
Aor51HI AGCGCT 1 cut(s) 1530
AoxI GGCC 5 cut(s) 1343, 1434, 1604, 2226, 2422
ApoI RAATTY 6 cut(s) 711, 802, 840, 1840, 1893, 2373
ArsI GACNNNNNNTTYG 2 cut(s) 2412, 2444
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 4 cut(s) 46, 427, 1531, 2436
AspS9I GGNCC 5 cut(s) 97, 1343, 1556, 1583, 2226
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 5 cut(s) 142, 499, 902, 1778, 2189
AvaII GGWCC 3 cut(s) 97, 1556, 1583
BanII GRGCYC 3 cut(s) 117, 138, 471
BbsI GAAGAC 1 cut(s) 2361
Bbv12I GWGCWC 2 cut(s) 117, 138
BbvCI CCTCAGC 1 cut(s) 846
BccI CCATC 2 cut(s) 632, 2237
BciT130I CCWGG 6 cut(s) 1139, 1370, 1624, 1953, 2220, 2426
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 7 cut(s) 1014, 1064, 1202, 2130, 2135, 2340, 2393
BfaI CTAG 3 cut(s) 17, 215, 2264
BfmI CTRYAG 3 cut(s) 657, 942, 1980
BfoI RGCGCY 1 cut(s) 1532
BfuAI ACCTGC 1 cut(s) 1224
BglII AGATCT 1 cut(s) 1388
BlpI GCTNAGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 7 cut(s) 309, 1139, 1370, 1624, 1953, 2220, 2426
Bme18I GGWCC 3 cut(s) 97, 1556, 1583
BmgT120I GGNCC 5 cut(s) 97, 1343, 1556, 1583, 2226
BmiI GGNNCC 2 cut(s) 468, 643
BmrFI CCNGG 7 cut(s) 309, 1139, 1370, 1624, 1953, 2220, 2426
BmsI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
BpiI GAAGAC 1 cut(s) 2361
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 846
Bpu1102I GCTNAGC 1 cut(s) 285
BpuEI CTTGAG 3 cut(s) 1046, 2083, 2112
BpuMI CCSGG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 2188
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 3 cut(s) 1014, 1064, 2393
BsaJI CCNNGG 3 cut(s) 308, 475, 549
BsaWI WCCGGW 2 cut(s) 251, 1427
BsaXI ACNNNNNCTCC 4 cut(s) 642, 672, 1863, 1893
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 477, 1918
Bse118I RCCGGY 2 cut(s) 152, 1788
Bse1I ACTGG 4 cut(s) 645, 676, 1179, 2150
Bse3DI GCAATG 3 cut(s) 762, 1818, 1935
Bse8I GATNNNNATC 1 cut(s) 264
BseAI TCCGGA 1 cut(s) 1427
BseBI CCWGG 6 cut(s) 1139, 1370, 1624, 1953, 2220, 2426
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 3 cut(s) 308, 475, 549
BseGI GGATG 4 cut(s) 307, 643, 1021, 1129
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 476, 477, 1918
BseMI GCAATG 3 cut(s) 762, 1818, 1935
BseMII CTCAG 7 cut(s) 299, 860, 1025, 1233, 1341, 1788, 2130
BseNI ACTGG 4 cut(s) 645, 676, 1179, 2150
BseRI GAGGAG 2 cut(s) 128, 199
BsgI GTGCAG 2 cut(s) 750, 812
Bsh1236I CGCG 2 cut(s) 44, 425
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 5 cut(s) 1345, 1436, 1606, 2228, 2424
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 117, 138
BsiSI CCGG 5 cut(s) 153, 252, 308, 1428, 1789
BslFI GGGAC 4 cut(s) 493, 664, 1018, 1478
BslI CCNNNNNNNGG 3 cut(s) 476, 477, 1918
BsmAI GTCTC 7 cut(s) 1014, 1064, 1202, 2130, 2135, 2340, 2393
BsmBI CGTCTC 1 cut(s) 2340
BsmFI GGGAC 4 cut(s) 493, 664, 1018, 1478
BsmI GAATGC 1 cut(s) 2101
BsnI GGCC 5 cut(s) 1345, 1436, 1606, 2228, 2424
Bso31I GGTCTC 3 cut(s) 1014, 1064, 2393
Bsp1286I GDGCHC 3 cut(s) 117, 138, 471
Bsp13I TCCGGA 1 cut(s) 1427
Bsp1407I TGTACA 1 cut(s) 2360
Bsp1720I GCTNAGC 1 cut(s) 285
Bsp19I CCATGG 1 cut(s) 549
BspANI GGCC 5 cut(s) 1345, 1436, 1606, 2228, 2424
BspCNI CTCAG 7 cut(s) 298, 859, 1024, 1234, 1342, 1787, 2131
BspDI ATCGAT 1 cut(s) 975
BspEI TCCGGA 1 cut(s) 1427
BspFNI CGCG 2 cut(s) 44, 425
BspHI TCATGA 2 cut(s) 1222, 2019
BspLI GGNNCC 2 cut(s) 468, 643
BspMI ACCTGC 1 cut(s) 1224
BspPI GGATC 8 cut(s) 299, 727, 980, 1306, 1559, 1963, 2141, 2278
BspQI GCTCTTC 1 cut(s) 2214
BspTNI GGTCTC 3 cut(s) 1014, 1064, 2393
BsrBI CCGCTC 1 cut(s) 2052
BsrDI GCAATG 3 cut(s) 762, 1818, 1935
BsrFI RCCGGY 2 cut(s) 152, 1788
BsrGI TGTACA 1 cut(s) 2360
BsrI ACTGG 4 cut(s) 645, 676, 1179, 2150
BssAI RCCGGY 2 cut(s) 152, 1788
BssECI CCNNGG 3 cut(s) 308, 475, 549
BssNAI GTATAC 1 cut(s) 519
BssT1I CCWWGG 2 cut(s) 475, 549
Bst1107I GTATAC 1 cut(s) 519
Bst2UI CCWGG 6 cut(s) 1139, 1370, 1624, 1953, 2220, 2426
Bst4CI ACNGT 6 cut(s) 148, 345, 1186, 1258, 2311, 2360
Bst6I CTCTTC 3 cut(s) 1429, 2040, 2214
BstAPI GCANNNNNTGC 3 cut(s) 419, 533, 625
BstAUI TGTACA 1 cut(s) 2360
BstBAI YACGTR 1 cut(s) 2188
BstC8I GCNNGC 5 cut(s) 51, 282, 1347, 2057, 2412
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 307, 643, 1021, 1129
BstFNI CGCG 2 cut(s) 44, 425
BstH2I RGCGCY 1 cut(s) 1532
BstHHI GCGC 4 cut(s) 46, 427, 1531, 2436
BstMAI GTCTC 7 cut(s) 1014, 1064, 1202, 2130, 2135, 2340, 2393
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 8 cut(s) 22, 50, 112, 286, 419, 533, 625, 2213
BstNI CCWGG 6 cut(s) 1139, 1370, 1624, 1953, 2220, 2426
BstNSI RCATGY 3 cut(s) 293, 437, 1852
BstSCI CCNGG 7 cut(s) 307, 1137, 1368, 1622, 1951, 2218, 2424
BstSFI CTRYAG 3 cut(s) 657, 942, 1980
BstSNI TACGTA 1 cut(s) 2188
BstUI CGCG 2 cut(s) 44, 425
BstV2I GAAGAC 1 cut(s) 2361
BstX2I RGATCY 3 cut(s) 1388, 2146, 2283
BstXI CCANNNNNNTGG 2 cut(s) 652, 1186
BstYI RGATCY 3 cut(s) 1388, 2146, 2283
BstZ17I GTATAC 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 975
BsuRI GGCC 5 cut(s) 1345, 1436, 1606, 2228, 2424
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 2215
BtsCI GGATG 4 cut(s) 307, 643, 1021, 1129
BtsIMutI CAGTG 2 cut(s) 652, 2316
BveI ACCTGC 1 cut(s) 1224
Cac8I GCNNGC 5 cut(s) 51, 282, 1347, 2057, 2412
CaiI CAGNNNCTG 2 cut(s) 622, 682
CciI TCATGA 2 cut(s) 1222, 2019
CfoI GCGC 4 cut(s) 46, 427, 1531, 2436
Cfr10I RCCGGY 2 cut(s) 152, 1788
Cfr13I GGNCC 5 cut(s) 97, 1343, 1556, 1583, 2226
ClaI ATCGAT 1 cut(s) 975
Csp6I GTAC 3 cut(s) 1182, 2189, 2361
CviAII CATG 8 cut(s) 269, 290, 434, 550, 1223, 1333, 1849, 2020
CviQI GTAC 3 cut(s) 1182, 2189, 2361
DraIII CACNNNGTG 1 cut(s) 728
Eam1104I CTCTTC 3 cut(s) 1429, 2040, 2214
EarI CTCTTC 3 cut(s) 1429, 2040, 2214
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 2 cut(s) 115, 136
Eco105I TACGTA 1 cut(s) 2188
Eco130I CCWWGG 2 cut(s) 475, 549
Eco147I AGGCCT 2 cut(s) 1606, 2424
Eco24I GRGCYC 3 cut(s) 117, 138, 471
Eco31I GGTCTC 3 cut(s) 1014, 1064, 2393
Eco32I GATATC 1 cut(s) 875
Eco47I GGWCC 3 cut(s) 97, 1556, 1583
Eco47III AGCGCT 1 cut(s) 1530
Eco53kI GAGCTC 2 cut(s) 115, 136
Eco57I CTGAAG 4 cut(s) 92, 1482, 1883, 2273
EcoICRI GAGCTC 2 cut(s) 115, 136
EcoO109I RGGNCCY 1 cut(s) 1556
EcoRI GAATTC 2 cut(s) 802, 840
EcoRII CCWGG 6 cut(s) 1137, 1368, 1622, 1951, 2218, 2424
EcoRV GATATC 1 cut(s) 875
EcoT14I CCWWGG 2 cut(s) 475, 549
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 3 cut(s) 117, 138, 471
ErhI CCWWGG 2 cut(s) 475, 549
Esp3I CGTCTC 1 cut(s) 2340
FaeI CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2023
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 4 cut(s) 493, 664, 1018, 1478
FatI CATG 8 cut(s) 268, 289, 433, 549, 1222, 1332, 1848, 2019
FauI CCCGC 3 cut(s) 479, 1998, 2419
FauNDI CATATG 2 cut(s) 529, 1096
FblI GTMKAC 2 cut(s) 518, 1238
FokI GGATG 4 cut(s) 314, 650, 1028, 1136
FriOI GRGCYC 3 cut(s) 117, 138, 471
FspBI CTAG 3 cut(s) 17, 215, 2264
FspI TGCGCA 1 cut(s) 2435
GlaI GCGC 4 cut(s) 45, 426, 1530, 2435
GsuI CTGGAG 1 cut(s) 1160
HaeII RGCGCY 1 cut(s) 1532
HaeIII GGCC 5 cut(s) 1345, 1436, 1606, 2228, 2424
HapII CCGG 5 cut(s) 153, 252, 308, 1428, 1789
HhaI GCGC 4 cut(s) 46, 427, 1531, 2436
Hin1II CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2023
Hin6I GCGC 4 cut(s) 44, 425, 1529, 2434
HinP1I GCGC 4 cut(s) 44, 425, 1529, 2434
HincII GTYRAC 2 cut(s) 1239, 2322
HindII GTYRAC 2 cut(s) 1239, 2322
HindIII AAGCTT 1 cut(s) 104
HpaI GTTAAC 1 cut(s) 2322
HpaII CCGG 5 cut(s) 153, 252, 308, 1428, 1789
HphI GGTGA 5 cut(s) 142, 499, 902, 1778, 2189
Hpy166II GTNNAC 7 cut(s) 212, 519, 910, 1239, 1650, 2197, 2322
Hpy8I GTNNAC 7 cut(s) 212, 519, 910, 1239, 1650, 2197, 2322
Hpy99I CGWCG 3 cut(s) 11, 89, 92
HpyCH4III ACNGT 6 cut(s) 148, 345, 1186, 1258, 2311, 2360
HpyCH4IV ACGT 4 cut(s) 84, 961, 2187, 2324
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 50, 112, 286, 419, 533, 625, 2213
HpySE526I ACGT 4 cut(s) 84, 961, 2187, 2324
Hsp92II CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2023
HspAI GCGC 4 cut(s) 44, 425, 1529, 2434
Kpn2I TCCGGA 1 cut(s) 1427
KspAI GTTAAC 1 cut(s) 2322
LguI GCTCTTC 1 cut(s) 2214
LmnI GCTCC 7 cut(s) 112, 120, 141, 186, 466, 641, 2389
LweI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
MaeI CTAG 3 cut(s) 17, 215, 2264
MaeII ACGT 4 cut(s) 84, 961, 2187, 2324
MaeIII GTNAC 6 cut(s) 148, 541, 612, 895, 1262, 2311
MbiI CCGCTC 1 cut(s) 2052
MfeI CAATTG 2 cut(s) 179, 780
MflI RGATCY 3 cut(s) 1388, 2146, 2283
MhlI GDGCHC 3 cut(s) 117, 138, 471
MlyI GAGTC 4 cut(s) 1100, 1253, 1451, 1718
MmeI TCCRAC 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 1021
MroI TCCGGA 1 cut(s) 1427
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2321, 2327
MslI CAYNNNNRTG 1 cut(s) 601
MspA1I CMGCKG 5 cut(s) 622, 1110, 1289, 1349, 1454
MspI CCGG 5 cut(s) 153, 252, 308, 1428, 1789
MspR9I CCNGG 7 cut(s) 309, 1139, 1370, 1624, 1953, 2220, 2426
MunI CAATTG 2 cut(s) 179, 780
Mva1269I GAATGC 1 cut(s) 2101
MvaI CCWGG 6 cut(s) 1139, 1370, 1624, 1953, 2220, 2426
MvnI CGCG 2 cut(s) 44, 425
MwoI GCNNNNNNNGC 8 cut(s) 22, 50, 112, 286, 419, 533, 625, 2213
NciI CCSGG 1 cut(s) 309
NcoI CCATGG 1 cut(s) 549
NdeI CATATG 2 cut(s) 529, 1096
NlaIII CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2023
NlaIV GGNNCC 2 cut(s) 468, 643
NmuCI GTSAC 3 cut(s) 148, 1262, 2311
NsbI TGCGCA 1 cut(s) 2435
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 3 cut(s) 293, 437, 1852
PagI TCATGA 2 cut(s) 1222, 2019
PceI AGGCCT 2 cut(s) 1606, 2424
PciSI GCTCTTC 1 cut(s) 2214
PctI GAATGC 1 cut(s) 2101
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 8 cut(s) 322, 356, 776, 785, 977, 1048, 1901, 2270
PflFI GACNNNGTC 1 cut(s) 1243
PfoI TCCNGGA 2 cut(s) 1622, 1951
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 4 cut(s) 1099, 1252, 1450, 1717
PpsI GAGTC 4 cut(s) 1099, 1252, 1450, 1717
Ppu21I YACGTR 1 cut(s) 2188
PpuMI RGGWCCY 1 cut(s) 1556
PsiI TTATAA 1 cut(s) 698
Psp124BI GAGCTC 2 cut(s) 117, 138
Psp1406I AACGTT 1 cut(s) 2324
Psp5II RGGWCCY 1 cut(s) 1556
Psp6I CCWGG 6 cut(s) 1137, 1368, 1622, 1951, 2218, 2424
PspGI CCWGG 6 cut(s) 1137, 1368, 1622, 1951, 2218, 2424
PspN4I GGNNCC 2 cut(s) 468, 643
PspPI GGNCC 5 cut(s) 97, 1343, 1556, 1583, 2226
PspPPI RGGWCCY 1 cut(s) 1556
PsrI GAACNNNNNNTAC 2 cut(s) 1795, 1827
PstNI CAGNNNCTG 2 cut(s) 622, 682
PsuI RGATCY 3 cut(s) 1388, 2146, 2283
PsyI GACNNNGTC 1 cut(s) 1243
PvuI CGATCG 1 cut(s) 79
PvuII CAGCTG 3 cut(s) 622, 1349, 1454
RsaI GTAC 3 cut(s) 1183, 2190, 2362
RsaNI GTAC 3 cut(s) 1182, 2189, 2361
RseI CAYNNNNRTG 1 cut(s) 601
SacI GAGCTC 2 cut(s) 117, 138
SalI GTCGAC 1 cut(s) 1237
SapI GCTCTTC 1 cut(s) 2214
SaqAI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2321, 2327
Sau96I GGNCC 5 cut(s) 97, 1343, 1556, 1583, 2226
ScaI AGTACT 1 cut(s) 1183
SchI GAGTC 4 cut(s) 1100, 1253, 1451, 1718
ScrFI CCNGG 7 cut(s) 309, 1139, 1370, 1624, 1953, 2220, 2426
SduI GDGCHC 3 cut(s) 117, 138, 471
SfaNI GCATC 8 cut(s) 72, 464, 918, 925, 940, 1006, 1028, 1801
SfcI CTRYAG 3 cut(s) 657, 942, 1980
SinI GGWCC 3 cut(s) 97, 1556, 1583
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 3 cut(s) 1061, 2062, 2127
SmoI CTYRAG 3 cut(s) 1061, 2062, 2127
SnaBI TACGTA 1 cut(s) 2188
SseBI AGGCCT 2 cut(s) 1606, 2424
SspMI CTAG 3 cut(s) 17, 215, 2264
SstI GAGCTC 2 cut(s) 117, 138
StuI AGGCCT 2 cut(s) 1606, 2424
StyD4I CCNGG 7 cut(s) 307, 1137, 1368, 1622, 1951, 2218, 2424
StyI CCWWGG 2 cut(s) 475, 549
TaaI ACNGT 6 cut(s) 148, 345, 1186, 1258, 2311, 2360
TaiI ACGT 4 cut(s) 87, 964, 2190, 2327
TatI WGTACW 2 cut(s) 1181, 2360
TauI GCSGC 4 cut(s) 425, 1113, 1439, 2055
TfiI GAWTC 8 cut(s) 322, 356, 776, 785, 977, 1048, 1901, 2270
Tru1I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2321, 2327
Tru9I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2321, 2327
TscAI CASTG 2 cut(s) 652, 2316
TseFI GTSAC 3 cut(s) 148, 1262, 2311
Tsp45I GTSAC 3 cut(s) 148, 1262, 2311
TspDTI ATGAA 8 cut(s) 657, 728, 799, 1266, 1529, 1749, 2008, 2283
TspRI CASTG 2 cut(s) 652, 2316
Tth111I GACNNNGTC 1 cut(s) 1243
VpaK11BI GGWCC 3 cut(s) 97, 1556, 1583
XapI RAATTY 6 cut(s) 711, 802, 840, 1840, 1893, 2373
XceI RCATGY 3 cut(s) 293, 437, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XmiI GTMKAC 2 cut(s) 518, 1238
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 3 cut(s) 17, 215, 2264
ZrmI AGTACT 1 cut(s) 1183
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.