Rh5CG446200

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007320
Physical Location & Seq
Reverse (-)
61918876 .. 61927107
8232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG446200.1

Sequence Viewer

Length: 2559 bp
ATGAGTCGGGTCGAAACGTCAAAACGGAGAGGAGAGAAGGCGAAGCCAAACCGATCCGTCACTCTCCCAGAGAGTCTCAACACAGTGATCGGAAAAACAAAAATGGACGACGAATTGGTGGAATTGCAGAAGCAATTCGAGTCGGCACAGCAAGCGAAATCGAGCATCAGATTGTCCGATCGAAACGTCGTCGAATTGGTCCAAAAGCTTCACGAGCTCCACATCATCGACTTCGACCTCCTCCACACCGTCTCCGGCAAAGAATACATCACTCCGGATCAATTGAGGCATGAAATACTGGTGGAGGTCAAGAGGTTGGGCCGTATATCACTGATTGACCTTGCAGATGCTATTGGGGTTGATTTGTACCATGTCGAGAAGCAAGCTCAACATGTGGTTTCTGATGATCCGGGGCTTATGTTGATTCAGGGGGAAATAATATCACAGTCCTATTGGGATTCCGTCGCTGAAGAAATCAATGAGAGGCTTCAAGAATGTAGCCAAGTTGCTTTGGCAGAACTTGCCGCGCAGTTACATGTCAGTTCTGAAATGGTGACATCTGTGTTGGAGCCGCGCCTTGGGACTACGGTGAAAGGTAGGCTTGAAGGTGGGCAATTGTATACCCCTGCTTATGTTGCACGTGTCACTGCCATGGTTCGTGGTGCTGCTAGGGCTATCACAGTTCCCACAAACTTATCAGTCTTGTGGAGTTCTTTACAGCAGTTGCTGCAAGAAATGGAGGGAGCCAGTGGTGTAGCTGTAGAAGGTTCATTTTTCCAATCCCTTTTTAACGGGCTTGTAAAGGAAGGCGAGATTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCAAATGTTTTTGCCATTGCTCAGAAGGAATCAATTGATTCTTTCTTCTCACAGAACTCTTTCATTAGCTATGACGTTCTGCAGAAGCTTAGAATTCCCCAGCCTGATCAGTTCTTGCAGTCCAGATATCCTGAATGCATACCTTTGGTTACTACATTTATTCACCCATCAATGATTGAGATGCTTGATGCTGCTATAGAAGATGCTCTTGAGCGTGGGAGCTGGATGGATTCTCTTTCTATACTGCCAATGTCCTTTGGATCTCAAGATGCATCCAAACTTTTGTCCCTTTGTCCTTCCATTCAGCAGGGCCTCAAGTCTGATAAGGCAATAATCTTCGGGGAATCCTTTGTATTTAGCAGTGCCTTTATCAAGGATGTGTACGATCGCCTGGAGAAAGAAATGGAAACCTTTATAGTTTCGAGTACTTCTGGTACTATGGTGTCAGATGATTTGCAGGAGACAAAAGTTGGTCACGAAACAAGCAGATTCACCGAATCAAATGAAACGGTTAGTGACAGCAGCAGTAATAAACAGACCATGGAGAAAGGATCAAAGAAGAAAAAGGGTCGAGTGGCTGGAAATATGGGAGCAGGTGCGGCTGAAGGTGATCCGGAAAACCAGGACTCTGTTCCTACAAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATTCCTCCTCCGCACAAGTTGCTGACTCAAAAGCATCTGCTAAGTTGGTAAAATCTAAAGAGGAAAATCTGAACATTCCTTCAGAAGATTGGATGATGAACAAGATTGCAACACTGGTTCCTGAATTCGAAGAACAAGGTCTTGATGATCCACAAACAATTATTAGACCTTTGGCAAACTATGTGAGACCTATGCTAATAAACTCTTGGAAGGAGAGAAGAAAGGCATTGTTTACTGAAAATGCAGAGAGAATGAAGCACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAACATGCAGCTCTACGAAAAAGCTTTAGATTTGTTTGAAGATGACCCATCAACTTCAGTCATTTTGCACCGGCATTTGTTAAGAACAACAGCTAGTACTATTGTGGATATGCTTCTTCTTAATTTGGACATGCACAACAAATTGAAGAATGGAGTTGAAGTTGAGGACACTCAGATTTCAGAATCTTCTTCACTTAACCCAGGAGAAAGAACTTCCATTGCTAAGAATTTTCCTGGATCTCTCTCAAAGAAGGCTCTTGTTGTTGTTGAAGCTTTGGAAGGAAAGCGGGTGGAAACATTCATGACTGCTTTAAGAGACATAGCTGAAGAAAGTGGTTTACTCCTGAAAAAGCTTGACAAGAAGTTGGAAAGAACACTTCTGCATTCGTATCAAAAGGATTTGGTGTCTCAAGTTTCTGCTGAAATGGATCCAGTCTCTGTTTTGGCCAAAGTTGTTTCCCTGCTATATGTACAGATTCACCATAAAGCTCTCCAAGCACCTGGAAGGGCCATCGCTGTTGCTGTTTCTCGGCTGAAGGATAAACTAGATGAATCAGCTTTCAAGATCCTGACAGAATATCAAACTGCAACAGTAACCCTCTTAGCACTAATATCCGCTGCAAGTGGAGATGAAGAAGACTGTACATCTGATAGAATATTGAGCAAAAGGGAGCTCCTGGAAAATCAAATGCCTGCACTGAAAGGCCTGGTTTTGCGCACTGGCACCTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

852

Amino Acids

94.58

Weight (kDa)

5.55

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 38 - 322 5.3e-100 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 339 - 386 1.4e-09 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 595 - 725 4.1e-39 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 730 - 843 1.4e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1433
Acc16I TGCGCA 1 cut(s) 2537
Acc36I ACCTGC 1 cut(s) 1433
AccB1I GGYRCC 1 cut(s) 2543
AccI GTMKAC 1 cut(s) 620
AccII CGCG 2 cut(s) 527, 574
AccIII TCCGGA 2 cut(s) 274, 1462
AciI CCGC 6 cut(s) 525, 572, 1448, 1530, 2107, 2436
AcoI YGGCCR 1 cut(s) 2265
AcsI RAATTY 4 cut(s) 942, 1519, 1643, 2047
AcuI CTGAAG 6 cut(s) 489, 1473, 1584, 1860, 2166, 2375
AcvI CACGTG 1 cut(s) 641
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 7 cut(s) 368, 1232, 1276, 1285, 1918, 2292, 2464
AfiI CCNNNNNNNGG 1 cut(s) 578
AflIII ACRYGT 3 cut(s) 391, 535, 640
AgsI TTSAA 7 cut(s) 491, 605, 1859, 1966, 1979, 2090, 2383
AjnI CCWGG 7 cut(s) 1239, 1470, 2020, 2053, 2320, 2496, 2526
Alw21I GWGCWC 2 cut(s) 219, 2496
Alw26I GTCTC 7 cut(s) 80, 256, 1304, 1699, 2130, 2232, 2260
AlwNI CAGNNNCTG 2 cut(s) 727, 2258
Aor13HI TCCGGA 2 cut(s) 274, 1462
AoxI GGCC 5 cut(s) 319, 1159, 2265, 2328, 2524
ApeKI GCWGC 6 cut(s) 665, 727, 1040, 1371, 1828, 2438
ApoI RAATTY 4 cut(s) 942, 1519, 1643, 2047
Asp700I GAANNNNTTC 2 cut(s) 134, 908
AspLEI GCGC 3 cut(s) 529, 576, 2538
AspS9I GGNCC 4 cut(s) 199, 319, 1159, 2328
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 6 cut(s) 565, 601, 1004, 1333, 1469, 2291
AsuII TTCGAA 1 cut(s) 1647
AvaII GGWCC 1 cut(s) 199
BaeI ACNNNNGTAYC 2 cut(s) 1275, 1308
BalI TGGCCA 1 cut(s) 2267
BamHI GGATCC 1 cut(s) 2248
BanI GGYRCC 1 cut(s) 2543
BanII GRGCYC 2 cut(s) 219, 2496
BauI CACGAG 1 cut(s) 212
BbrPI CACGTG 1 cut(s) 641
BbsI GAAGAC 1 cut(s) 2463
Bbv12I GWGCWC 2 cut(s) 219, 2496
BbvI GCAGC 6 cut(s) 652, 714, 1027, 1383, 1840, 2425
BccI CCATC 4 cut(s) 1024, 1069, 1876, 2339
BceAI ACGGC 1 cut(s) 306
BciT130I CCWGG 7 cut(s) 1241, 1472, 2022, 2055, 2322, 2498, 2528
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 7 cut(s) 80, 256, 1304, 1699, 2130, 2232, 2260
BfaI CTAG 3 cut(s) 669, 1914, 2366
BfmI CTRYAG 3 cut(s) 759, 929, 1044
BfuAI ACCTGC 1 cut(s) 1433
BisI GCNGC 9 cut(s) 525, 572, 666, 728, 1041, 1372, 1449, 1829, 2439
BlsI GCNGC 9 cut(s) 526, 573, 667, 729, 1042, 1373, 1450, 1830, 2440
BmcAI AGTACT 2 cut(s) 1276, 1918
Bme1390I CCNGG 8 cut(s) 411, 1241, 1472, 2022, 2055, 2322, 2498, 2528
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 4 cut(s) 199, 319, 1159, 2328
BmiI GGNNCC 5 cut(s) 570, 745, 1638, 2250, 2545
BmrFI CCNGG 8 cut(s) 411, 1241, 1472, 2022, 2055, 2322, 2498, 2528
BmsI GCATC 8 cut(s) 174, 337, 1020, 1027, 1042, 1108, 1130, 1562
BpiI GAAGAC 1 cut(s) 2463
BpmI CTGGAG 1 cut(s) 1262
Bpu14I TTCGAA 1 cut(s) 1647
BpuEI CTTGAG 4 cut(s) 1079, 1098, 1148, 2214
BpuMI CCSGG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 641
BsaI GGTCTC 1 cut(s) 1699
BsaJI CCNNGG 5 cut(s) 410, 577, 651, 1389, 2020
BsaWI WCCGGW 2 cut(s) 274, 1462
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 1965, 1995
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse118I RCCGGY 1 cut(s) 1890
Bse1I ACTGG 5 cut(s) 303, 747, 1638, 2252, 2545
Bse3DI GCAATG 2 cut(s) 864, 2037
BseAI TCCGGA 2 cut(s) 274, 1462
BseBI CCWGG 7 cut(s) 1241, 1472, 2022, 2055, 2322, 2498, 2528
BseDI CCNNGG 5 cut(s) 410, 577, 651, 1389, 2020
BseGI GGATG 4 cut(s) 1080, 1121, 1231, 1617
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMI GCAATG 2 cut(s) 864, 2037
BseMII CTCAG 2 cut(s) 884, 2006
BseNI ACTGG 5 cut(s) 303, 747, 1638, 2252, 2545
BseRI GAGGAG 3 cut(s) 45, 230, 1516
BseXI GCAGC 6 cut(s) 652, 714, 1027, 1383, 1840, 2425
BseYI CCCAGC 1 cut(s) 948
BsgI GTGCAG 2 cut(s) 852, 2499
Bsh1236I CGCG 2 cut(s) 527, 574
Bsh1285I CGRYCG 2 cut(s) 181, 1237
BshFI GGCC 5 cut(s) 321, 1161, 2267, 2330, 2526
BshNI GGYRCC 1 cut(s) 2543
BsiEI CGRYCG 2 cut(s) 181, 1237
BsiHKAI GWGCWC 2 cut(s) 219, 2496
BsiSI CCGG 5 cut(s) 255, 275, 410, 1463, 1891
BslFI GGGAC 2 cut(s) 595, 1120
BslI CCNNNNNNNGG 1 cut(s) 578
BsmAI GTCTC 7 cut(s) 80, 256, 1304, 1699, 2130, 2232, 2260
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 2 cut(s) 595, 1120
BsmI GAATGC 2 cut(s) 989, 2203
BsnI GGCC 5 cut(s) 321, 1161, 2267, 2330, 2526
Bso31I GGTCTC 1 cut(s) 1699
Bsp119I TTCGAA 1 cut(s) 1647
Bsp1286I GDGCHC 2 cut(s) 219, 2496
Bsp13I TCCGGA 2 cut(s) 274, 1462
Bsp1407I TGTACA 2 cut(s) 2290, 2462
Bsp19I CCATGG 2 cut(s) 651, 1389
BspACI CCGC 6 cut(s) 525, 572, 1448, 1530, 2107, 2436
BspANI GGCC 5 cut(s) 321, 1161, 2267, 2330, 2526
BspCNI CTCAG 2 cut(s) 883, 2005
BspEI TCCGGA 2 cut(s) 274, 1462
BspFNI CGCG 2 cut(s) 527, 574
BspHI TCATGA 1 cut(s) 2121
BspLI GGNNCC 5 cut(s) 570, 745, 1638, 2250, 2545
BspMAI CTGCAG 1 cut(s) 933
BspMI ACCTGC 1 cut(s) 1433
BspT104I TTCGAA 1 cut(s) 1647
BspT107I GGYRCC 1 cut(s) 2543
BspTNI GGTCTC 1 cut(s) 1699
BsrDI GCAATG 2 cut(s) 864, 2037
BsrFI RCCGGY 1 cut(s) 1890
BsrGI TGTACA 2 cut(s) 2290, 2462
BsrI ACTGG 5 cut(s) 303, 747, 1638, 2252, 2545
BssAI RCCGGY 1 cut(s) 1890
BssECI CCNNGG 5 cut(s) 410, 577, 651, 1389, 2020
BssNAI GTATAC 1 cut(s) 621
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 577, 651, 1389
Bst1107I GTATAC 1 cut(s) 621
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 7 cut(s) 1241, 1472, 2022, 2055, 2322, 2498, 2528
Bst4CI ACNGT 8 cut(s) 85, 250, 447, 589, 682, 1360, 2413, 2462
BstAPI GCANNNNNTGC 3 cut(s) 521, 727, 1538
BstAUI TGTACA 2 cut(s) 2290, 2462
BstBAI YACGTR 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 1647
BstC8I GCNNGC 3 cut(s) 153, 384, 2514
BstDEI CTNAG 7 cut(s) 870, 938, 1494, 1560, 1992, 2043, 2422
BstDSI CCRYGG 2 cut(s) 651, 1389
BstF5I GGATG 4 cut(s) 1080, 1121, 1231, 1617
BstFNI CGCG 2 cut(s) 527, 574
BstHHI GCGC 3 cut(s) 529, 576, 2538
BstMAI GTCTC 7 cut(s) 80, 256, 1304, 1699, 2130, 2232, 2260
BstMCI CGRYCG 2 cut(s) 181, 1237
BstMWI GCNNNNNNNGC 9 cut(s) 152, 214, 521, 635, 671, 727, 1448, 1538, 2315
BstNI CCWGG 7 cut(s) 1241, 1472, 2022, 2055, 2322, 2498, 2528
BstNSI RCATGY 4 cut(s) 395, 539, 1828, 1954
BstSCI CCNGG 8 cut(s) 409, 1239, 1470, 2020, 2053, 2320, 2496, 2526
BstSFI CTRYAG 3 cut(s) 759, 929, 1044
BstUI CGCG 2 cut(s) 527, 574
BstV1I GCAGC 6 cut(s) 652, 714, 1027, 1383, 1840, 2425
BstV2I GAAGAC 1 cut(s) 2463
BstX2I RGATCY 4 cut(s) 1109, 2057, 2248, 2385
BstXI CCANNNNNNTGG 1 cut(s) 2321
BstYI RGATCY 4 cut(s) 1109, 2057, 2248, 2385
BstZ17I GTATAC 1 cut(s) 621
BsuRI GGCC 5 cut(s) 321, 1161, 2267, 2330, 2526
BtgI CCRYGG 2 cut(s) 651, 1389
BtgZI GCGATG 1 cut(s) 2317
BtsCI GGATG 4 cut(s) 1080, 1121, 1231, 1617
BtsI GCAGTG 2 cut(s) 645, 1216
BtsIMutI CAGTG 8 cut(s) 90, 329, 645, 754, 1216, 1631, 2516, 2538
BveI ACCTGC 1 cut(s) 1433
Cac8I GCNNGC 3 cut(s) 153, 384, 2514
CaiI CAGNNNCTG 2 cut(s) 727, 2258
CciI TCATGA 1 cut(s) 2121
CfoI GCGC 3 cut(s) 529, 576, 2538
Cfr10I RCCGGY 1 cut(s) 1890
Cfr13I GGNCC 4 cut(s) 199, 319, 1159, 2328
Csp6I GTAC 7 cut(s) 367, 1231, 1275, 1284, 1917, 2291, 2463
CviAII CATG 9 cut(s) 290, 371, 392, 536, 652, 1390, 1825, 1951, 2122
CviQI GTAC 7 cut(s) 367, 1231, 1275, 1284, 1917, 2291, 2463
DdeI CTNAG 7 cut(s) 870, 938, 1494, 1560, 1992, 2043, 2422
DraIII CACNNNGTG 1 cut(s) 830
EaeI YGGCCR 1 cut(s) 2265
Ecl136II GAGCTC 2 cut(s) 217, 2494
Eco130I CCWWGG 3 cut(s) 577, 651, 1389
Eco147I AGGCCT 1 cut(s) 2526
Eco24I GRGCYC 2 cut(s) 219, 2496
Eco31I GGTCTC 1 cut(s) 1699
Eco32I GATATC 1 cut(s) 977
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 2 cut(s) 217, 2494
Eco57I CTGAAG 6 cut(s) 489, 1473, 1584, 1860, 2166, 2375
Eco72I CACGTG 1 cut(s) 641
EcoICRI GAGCTC 2 cut(s) 217, 2494
EcoO109I RGGNCCY 1 cut(s) 1159
EcoRI GAATTC 3 cut(s) 942, 1519, 1643
EcoRII CCWGG 7 cut(s) 1239, 1470, 2020, 2053, 2320, 2496, 2526
EcoRV GATATC 1 cut(s) 977
EcoT14I CCWWGG 3 cut(s) 577, 651, 1389
EcoT22I ATGCAT 2 cut(s) 989, 1123
EcoT38I GRGCYC 2 cut(s) 219, 2496
ErhI CCWWGG 3 cut(s) 577, 651, 1389
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 9 cut(s) 293, 374, 395, 539, 655, 1393, 1828, 1954, 2125
FalI AAGNNNNNCTT 4 cut(s) 585, 617, 1041, 1073
FaqI GGGAC 2 cut(s) 595, 1120
FatI CATG 9 cut(s) 289, 370, 391, 535, 651, 1389, 1824, 1950, 2121
FauI CCCGC 1 cut(s) 2100
FbaI TGATCA 1 cut(s) 955
FblI GTMKAC 1 cut(s) 620
Fnu4HI GCNGC 9 cut(s) 525, 572, 666, 728, 1041, 1372, 1449, 1829, 2439
FokI GGATG 4 cut(s) 1087, 1108, 1238, 1624
FriOI GRGCYC 2 cut(s) 219, 2496
Fsp4HI GCNGC 9 cut(s) 525, 572, 666, 728, 1041, 1372, 1449, 1829, 2439
FspBI CTAG 3 cut(s) 669, 1914, 2366
FspI TGCGCA 1 cut(s) 2537
GlaI GCGC 3 cut(s) 528, 575, 2537
GluI GCNGC 9 cut(s) 525, 572, 666, 728, 1041, 1372, 1449, 1829, 2439
GsaI CCCAGC 1 cut(s) 952
GsuI CTGGAG 1 cut(s) 1262
HaeIII GGCC 5 cut(s) 321, 1161, 2267, 2330, 2526
HapII CCGG 5 cut(s) 255, 275, 410, 1463, 1891
HhaI GCGC 3 cut(s) 529, 576, 2538
Hin1II CATG 9 cut(s) 293, 374, 395, 539, 655, 1393, 1828, 1954, 2125
Hin6I GCGC 3 cut(s) 527, 574, 2536
HinP1I GCGC 3 cut(s) 527, 574, 2536
HindIII AAGCTT 5 cut(s) 206, 935, 1842, 2091, 2171
HpaII CCGG 5 cut(s) 255, 275, 410, 1463, 1891
HphI GGTGA 6 cut(s) 565, 601, 1004, 1333, 1469, 2291
Hpy166II GTNNAC 4 cut(s) 621, 1231, 1752, 2159
Hpy8I GTNNAC 4 cut(s) 621, 1231, 1752, 2159
Hpy99I CGWCG 4 cut(s) 113, 191, 194, 467
HpyCH4III ACNGT 8 cut(s) 85, 250, 447, 589, 682, 1360, 2413, 2462
HpyCH4IV ACGT 4 cut(s) 17, 186, 640, 924
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 214, 521, 635, 671, 727, 1448, 1538, 2315
HpyF3I CTNAG 7 cut(s) 870, 938, 1494, 1560, 1992, 2043, 2422
HpySE526I ACGT 4 cut(s) 17, 186, 640, 924
Hsp92II CATG 9 cut(s) 293, 374, 395, 539, 655, 1393, 1828, 1954, 2125
HspAI GCGC 3 cut(s) 527, 574, 2536
Kpn2I TCCGGA 2 cut(s) 274, 1462
Ksp22I TGATCA 1 cut(s) 955
LmnI GCTCC 7 cut(s) 222, 568, 743, 1068, 1439, 2491, 2499
Lsp1109I GCAGC 6 cut(s) 652, 714, 1027, 1383, 1840, 2425
LweI GCATC 8 cut(s) 174, 337, 1020, 1027, 1042, 1108, 1130, 1562
MaeI CTAG 3 cut(s) 669, 1914, 2366
MaeII ACGT 4 cut(s) 17, 186, 640, 924
MaeIII GTNAC 8 cut(s) 58, 531, 553, 643, 997, 1322, 1364, 2413
MfeI CAATTG 3 cut(s) 281, 614, 882
MflI RGATCY 4 cut(s) 1109, 2057, 2248, 2385
MhlI GDGCHC 2 cut(s) 219, 2496
MlsI TGGCCA 1 cut(s) 2267
MluNI TGGCCA 1 cut(s) 2267
MlyI GAGTC 6 cut(s) 13, 82, 149, 1469, 1538, 1820
MmeI TCCRAC 2 cut(s) 546, 2166
Mox20I TGGCCA 1 cut(s) 2267
Mph1103I ATGCAT 2 cut(s) 989, 1123
MroI TCCGGA 2 cut(s) 274, 1462
MroXI GAANNNNTTC 2 cut(s) 134, 908
MscI TGGCCA 1 cut(s) 2267
MseI TTAA 6 cut(s) 789, 1820, 1901, 1941, 2016, 2132
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 2267
MspA1I CMGCKG 1 cut(s) 2438
MspI CCGG 5 cut(s) 255, 275, 410, 1463, 1891
MspR9I CCNGG 8 cut(s) 411, 1241, 1472, 2022, 2055, 2322, 2498, 2528
MunI CAATTG 3 cut(s) 281, 614, 882
Mva1269I GAATGC 2 cut(s) 989, 2203
MvaI CCWGG 7 cut(s) 1241, 1472, 2022, 2055, 2322, 2498, 2528
MvnI CGCG 2 cut(s) 527, 574
MwoI GCNNNNNNNGC 9 cut(s) 152, 214, 521, 635, 671, 727, 1448, 1538, 2315
NciI CCSGG 1 cut(s) 411
NcoI CCATGG 2 cut(s) 651, 1389
NlaIII CATG 9 cut(s) 293, 374, 395, 539, 655, 1393, 1828, 1954, 2125
NlaIV GGNNCC 5 cut(s) 570, 745, 1638, 2250, 2545
NmeAIII GCCGAG 1 cut(s) 2329
NmuCI GTSAC 5 cut(s) 58, 553, 643, 1322, 1364
NsbI TGCGCA 1 cut(s) 2537
NsiI ATGCAT 2 cut(s) 989, 1123
NspI RCATGY 4 cut(s) 395, 539, 1828, 1954
NspV TTCGAA 1 cut(s) 1647
PagI TCATGA 1 cut(s) 2121
PaqCI CACCTGC 1 cut(s) 1433
PceI AGGCCT 1 cut(s) 2526
PciI ACATGT 2 cut(s) 391, 535
PctI GAATGC 2 cut(s) 989, 2203
PdmI GAANNNNTTC 2 cut(s) 134, 908
PfoI TCCNGGA 2 cut(s) 2053, 2496
PkrI GCNGC 9 cut(s) 526, 573, 667, 729, 1042, 1373, 1450, 1830, 2440
Ple19I CGATCG 2 cut(s) 181, 1237
PleI GAGTC 6 cut(s) 12, 81, 148, 1469, 1538, 1819
PmaCI CACGTG 1 cut(s) 641
PmlI CACGTG 1 cut(s) 641
PpsI GAGTC 6 cut(s) 12, 81, 148, 1469, 1538, 1819
Ppu21I YACGTR 1 cut(s) 641
PscI ACATGT 2 cut(s) 391, 535
Psp124BI GAGCTC 2 cut(s) 219, 2496
Psp6I CCWGG 7 cut(s) 1239, 1470, 2020, 2053, 2320, 2496, 2526
PspCI CACGTG 1 cut(s) 641
PspFI CCCAGC 1 cut(s) 948
PspGI CCWGG 7 cut(s) 1239, 1470, 2020, 2053, 2320, 2496, 2526
PspN4I GGNNCC 5 cut(s) 570, 745, 1638, 2250, 2545
PspPI GGNCC 4 cut(s) 199, 319, 1159, 2328
PstI CTGCAG 1 cut(s) 933
PstNI CAGNNNCTG 2 cut(s) 727, 2258
PsuI RGATCY 4 cut(s) 1109, 2057, 2248, 2385
PvuI CGATCG 2 cut(s) 181, 1237
RsaI GTAC 7 cut(s) 368, 1232, 1276, 1285, 1918, 2292, 2464
RsaNI GTAC 7 cut(s) 367, 1231, 1275, 1284, 1917, 2291, 2463
RseI CAYNNNNRTG 1 cut(s) 650
SacI GAGCTC 2 cut(s) 219, 2496
SaqAI TTAA 6 cut(s) 789, 1820, 1901, 1941, 2016, 2132
SatI GCNGC 9 cut(s) 525, 572, 666, 728, 1041, 1372, 1449, 1829, 2439
Sau96I GGNCC 4 cut(s) 199, 319, 1159, 2328
ScaI AGTACT 2 cut(s) 1276, 1918
SchI GAGTC 6 cut(s) 13, 82, 149, 1469, 1538, 1820
ScrFI CCNGG 8 cut(s) 411, 1241, 1472, 2022, 2055, 2322, 2498, 2528
SduI GDGCHC 2 cut(s) 219, 2496
SfaNI GCATC 8 cut(s) 174, 337, 1020, 1027, 1042, 1108, 1130, 1562
SfcI CTRYAG 3 cut(s) 759, 929, 1044
SfuI TTCGAA 1 cut(s) 1647
SinI GGWCC 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 4 cut(s) 1058, 1113, 1163, 2229
SmoI CTYRAG 4 cut(s) 1058, 1113, 1163, 2229
SseBI AGGCCT 1 cut(s) 2526
SsiI CCGC 6 cut(s) 525, 572, 1448, 1530, 2107, 2436
SspI AATATT 1 cut(s) 2478
SspMI CTAG 3 cut(s) 669, 1914, 2366
SstI GAGCTC 2 cut(s) 219, 2496
StuI AGGCCT 1 cut(s) 2526
StyD4I CCNGG 8 cut(s) 409, 1239, 1470, 2020, 2053, 2320, 2496, 2526
StyI CCWWGG 3 cut(s) 577, 651, 1389
TaaI ACNGT 8 cut(s) 85, 250, 447, 589, 682, 1360, 2413, 2462
TaiI ACGT 4 cut(s) 20, 189, 643, 927
TatI WGTACW 4 cut(s) 1274, 1916, 2290, 2462
TauI GCSGC 3 cut(s) 527, 574, 1451
Tru1I TTAA 6 cut(s) 789, 1820, 1901, 1941, 2016, 2132
Tru9I TTAA 6 cut(s) 789, 1820, 1901, 1941, 2016, 2132
TscAI CASTG 8 cut(s) 90, 336, 652, 754, 1216, 1638, 2523, 2545
TseFI GTSAC 5 cut(s) 58, 553, 643, 1322, 1364
TseI GCWGC 6 cut(s) 665, 727, 1040, 1371, 1828, 2438
Tsp45I GTSAC 5 cut(s) 58, 553, 643, 1322, 1364
TspGWI ACGGA 3 cut(s) 40, 46, 451
TspRI CASTG 8 cut(s) 90, 336, 652, 754, 1216, 1638, 2523, 2545
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 4 cut(s) 942, 1519, 1643, 2047
XceI RCATGY 4 cut(s) 395, 539, 1828, 1954
XmiI GTMKAC 1 cut(s) 620
XmnI GAANNNNTTC 2 cut(s) 134, 908
XspI CTAG 3 cut(s) 669, 1914, 2366
ZrmI AGTACT 2 cut(s) 1276, 1918
Zsp2I ATGCAT 2 cut(s) 989, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.