MD11G1116800.v1.1

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007320
Physical Location & Seq
Forward (+)
10564646 .. 10572504
7859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1116800.v1.1.491

Sequence Viewer

Length: 2454 bp
ATGGACGACGAATTGCTGGAACTGCAGAGGCAATTCGAGTTCGCACAGCAGGCGAAATCGAGCATCAGATTATCCGATCGAAACGTCGTCGAATTGGTCCAGAAGCTTCAGGAGCTGCATATCATCGACTTCGAGCTTCTCCACACCGTCACCGGCAAAGAATACATTACTCCCGATCAATTGAGGAATGAGATATCGGCGGAGGTCGGTAAACTAGGGCGTGTTTCGTTGATTGACCTGGCGGACGCTACCGGAGTTGATTTGTATCATGTTGAGAAGCAAGCTCAACATGTTGTTTCGGATGATCTGGGGCTTATGTTGATTCAAGGGGAAATAATATCGCAATCTTATTGGGATTCTGTCGCCGAAGAAGTCAATGACAGGCTTCAAGAGTGTAGCCAAATCGCTCTGGCAGAACTTGCCGCGCAGTTGCATGTCAGTTCGGAAATGGTGGCATCTGTGTTGGAGCCCCGCCTTGGGACTTTGGTGAAAGGTAGGCTTGAAGGTGGGCAATTGTATACTCCTGCATATGTTGCACGGGTTACTGCCATGGTTCGTGGTGCTGCCAGAGGTATCGCAGTTCCCACAAACTTATCATTGTTGTGGAGTTCTTTACAGCAGTTGCTGCAAGAAATGGATGGAGCGAGTGGAGTGGCTGTAGAAGGTTCTTTTTTCCAGTCCCTATTTAATGGGCTTGTAAAGGAAGGACAGCTTCTTGGATCACTTCGCGCAGGAGTTCATTGGACACCTAATGTCTTTGCCATTGCTCAAAAGGAATCAATTGATTCTTTCTTTTCACAGAATTCTTTCATTAACTATGATGTTCTGCACAAACTTAGAATTCCTCAGCCTATTCAGTTCTTGCAGTCCAGATATCCAGAAGGCATACCTTTGGTTACTACATTTGTTCACCCCTCAATGATTGAGATGCTTGATGCTGCTACGGAAGATGCTCTCGAACGTGATAGCTGGATCGATTCTCTTTCTATACTGCCGATGTCCTTTGGGTCTCAAGATGCGTCTAAACTGTTGTCCCTTTGCCCTTCCATTCAGCAGGGTCTCAAGTCTGATAAAGCAATAATATTCGGGGACTCGTATGTGTTTAGCAGTGGCTTTATCAAGGATGTGTATGATCTTCTAGAGAAAGAAATGGAAACCTTTAGTGTTTCAGTTCCTTCCAGTACTGTGGTGTCAGATGATTTGCGTGAGACAAAAATTGGTCATGACACAAGCAGGTCGACTGAGTCAAATGAAACTGTTAGTGACAGCAGCAGTAATAAACAGGCAACGGAGAAAGGATCAAAAAAGAAAAAAGGTAGAGGGGCTGGGAACATGATGACAGGGCCAGCTGAGAGTGAGCTGGACAACCAGGACAGTGTTCCTACGAAATCTAAGAAAAACCAAAGGAAGGGCAAGAATATCTCTTCCGGGCAGGCCACAGAGTCAAAAGCAGCTGCTAAGTTGGTGAAAATAAAAGAGGAAAATCTCAACGTCCCTTCAGAAGATTGGGTAATGAATAAGATTACAGTGCTAGTACCTGATTTTGAAGAACAAGGTCTTGACGGTCCCCAAACGATTCTTGGACCTTTGGCACACTATTTGAGGCCTAAGTTAATCAACTCCTGGAAGGAGAGAAGAAAGGCATTGTTTACAGAAAATGCGGAGAGAATGAAGAACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAATATGCAGCTTTATGAAAAGGCTTTAGATTTGTTTGAAGATGACCAATCAACCTCAGTCATTTTTCACAGGCATCTGTTAAGAACAACAGCTACTACTATTGCCGACATACTTCTTCACAATTTGGACATGCACAACAAATTGAAGAATGGAGTTGAAGAAGTTGCAGAGCCTCAAATTTCAGAATCTATTTCCCTCAATGCGGGAGAGAGAACTTCCATTGCCAAGAATTTTCCTGGATCTCTTTCAAACAAGGCTCTTGCTGTAGTTGAAGCATTGGAAGGAAAGCGGGTGGAAGCATTCATGACTGCTCTAAGAGATATTGCAGAAGAGAGCGGCTTGCTCTTGAAAAAGCTTGACAAGAAATTGGAAAGAACGCTTCTGCATTCATATCAGAAGGATTTGGTGTCTCAAGTTTCTGCTGAGACAGATCCAGTTCCTCTTTTGCCAAAAGTTGTTTCCCTGATTTACGTTCAGTTTCACCATAAAGCTCTTCAAGCACCTGGAAGGGCCATCGCTGTTGCTGTTTCACGGCTGAAGGATAAACTGGATGAATCAGCCTTCAAGATCCTGACAGATTATCAAACTGCAACAGTGACACTGTTAACGTTAATATCTGCTGCAAGTGTAGAGGAAGAAGATTGTTCATCTGATAGACTTTTGAACAGAAGGGAGCTTTTGGAGACTCAAATGCCTGCACTCAAAGGCCTGGTCTTACGCACCTCACAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

818

Amino Acids

90.55

Weight (kDa)

5.29

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 4 - 288 6.4e-97 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 305 - 353 1.1e-07 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 561 - 692 1e-38 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 697 - 809 1.5e-28 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1224
AccBSI CCGCTC 1 cut(s) 2055
AccI GTMKAC 2 cut(s) 518, 1238
AccII CGCG 2 cut(s) 425, 729
AciI CCGC 8 cut(s) 200, 242, 423, 472, 1661, 1922, 2008, 2055
AclI AACGTT 1 cut(s) 2327
AclWI GGATC 6 cut(s) 727, 980, 1306, 1966, 2144, 2281
AcsI RAATTY 4 cut(s) 802, 840, 1896, 1948
AcuI CTGAAG 3 cut(s) 92, 1482, 2276
AfaI GTAC 2 cut(s) 1183, 1536
AfiI CCNNNNNNNGG 4 cut(s) 476, 477, 1408, 1921
AflIII ACRYGT 1 cut(s) 289
AjnI CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
AjuI GAANNNNNNNTTGG 2 cut(s) 2161, 2193
Alw26I GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
AlwI GGATC 6 cut(s) 727, 980, 1306, 1966, 2144, 2281
AlwNI CAGNNNCTG 2 cut(s) 115, 625
AoxI GGCC 5 cut(s) 1343, 1434, 1604, 2229, 2425
ApeKI GCWGC 9 cut(s) 115, 563, 625, 938, 1269, 1451, 1454, 1726, 2339
ApoI RAATTY 4 cut(s) 802, 840, 1896, 1948
ArsI GACNNNNNNTTYG 2 cut(s) 2415, 2447
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 2 cut(s) 427, 731
AspS9I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
AsuC2I CCSGG 1 cut(s) 1429
AsuHPI GGTGA 5 cut(s) 142, 499, 902, 1477, 2192
AvaII GGWCC 3 cut(s) 97, 1565, 1583
BanII GRGCYC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1441, 1463, 1738, 2326
BccI CCATC 2 cut(s) 632, 2240
BceAI ACGGC 1 cut(s) 2267
BciT130I CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BcnI CCSGG 1 cut(s) 1429
BcoDI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BfaI CTAG 3 cut(s) 215, 1139, 1532
BfmI CTRYAG 3 cut(s) 23, 657, 1983
BfuAI ACCTGC 1 cut(s) 1224
BmcAI AGTACT 1 cut(s) 1183
Bme1390I CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
Bme18I GGWCC 3 cut(s) 97, 1565, 1583
BmgT120I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
BmiI GGNNCC 2 cut(s) 468, 1567
BmrFI CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
BmsI GCATC 7 cut(s) 72, 464, 918, 925, 940, 1006, 1801
Bpu10I CCTNAGC 1 cut(s) 846
BpuEI CTTGAG 3 cut(s) 996, 1046, 2115
BpuMI CCSGG 1 cut(s) 1429
Bsa29I ATCGAT 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 264
BsaI GGTCTC 2 cut(s) 1014, 1064
BsaJI CCNNGG 2 cut(s) 475, 549
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 642, 672
Bsc4I CCNNNNNNNGG 4 cut(s) 476, 477, 1408, 1921
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 4 cut(s) 676, 1179, 2153, 2271
Bse3DI GCAATG 2 cut(s) 762, 1938
Bse8I GATNNNNATC 1 cut(s) 264
BseBI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 2 cut(s) 475, 549
BseGI GGATG 4 cut(s) 307, 643, 1129, 2275
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 4 cut(s) 476, 477, 1408, 1921
BseMI GCAATG 2 cut(s) 762, 1938
BseMII CTCAG 5 cut(s) 860, 1233, 1341, 1788, 2133
BseNI ACTGG 4 cut(s) 676, 1179, 2153, 2271
BseXI GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1441, 1463, 1738, 2326
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 2 cut(s) 812, 2400
Bsh1236I CGCG 2 cut(s) 425, 729
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 5 cut(s) 1345, 1436, 1606, 2231, 2427
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 79
BsiSI CCGG 3 cut(s) 153, 252, 1428
BslFI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
BslI CCNNNNNNNGG 4 cut(s) 476, 477, 1408, 1921
BsmAI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BsmFI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
BsmI GAATGC 2 cut(s) 2018, 2104
BsnI GGCC 5 cut(s) 1345, 1436, 1606, 2231, 2427
Bso31I GGTCTC 2 cut(s) 1014, 1064
Bsp1286I GDGCHC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 8 cut(s) 200, 242, 423, 472, 1661, 1922, 2008, 2055
BspANI GGCC 5 cut(s) 1345, 1436, 1606, 2231, 2427
BspCNI CTCAG 5 cut(s) 859, 1234, 1342, 1787, 2134
BspDI ATCGAT 1 cut(s) 975
BspFNI CGCG 2 cut(s) 425, 729
BspHI TCATGA 2 cut(s) 1222, 2022
BspLI GGNNCC 2 cut(s) 468, 1567
BspMAI CTGCAG 1 cut(s) 27
BspMI ACCTGC 1 cut(s) 1224
BspPI GGATC 6 cut(s) 727, 980, 1306, 1966, 2144, 2281
BspQI GCTCTTC 1 cut(s) 2217
BspTNI GGTCTC 2 cut(s) 1014, 1064
BsrBI CCGCTC 1 cut(s) 2055
BsrDI GCAATG 2 cut(s) 762, 1938
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 4 cut(s) 676, 1179, 2153, 2271
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 475, 549
BssNAI GTATAC 1 cut(s) 519
BssT1I CCWWGG 2 cut(s) 475, 549
Bst1107I GTATAC 1 cut(s) 519
Bst2UI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
Bst4CI ACNGT 9 cut(s) 148, 1029, 1186, 1258, 1376, 1528, 1565, 2314, 2322
Bst6I CTCTTC 3 cut(s) 1429, 2043, 2217
BstAPI GCANNNNNTGC 3 cut(s) 419, 533, 625
BstC8I GCNNGC 6 cut(s) 51, 282, 1347, 1434, 2060, 2415
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 307, 643, 1129, 2275
BstFNI CGCG 2 cut(s) 425, 729
BstHHI GCGC 2 cut(s) 427, 731
BstMAI GTCTC 6 cut(s) 1014, 1064, 1202, 2133, 2138, 2396
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
BstNI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
BstNSI RCATGY 3 cut(s) 293, 437, 1852
BstSCI CCNGG 7 cut(s) 237, 1368, 1427, 1622, 1954, 2221, 2427
BstSFI CTRYAG 3 cut(s) 23, 657, 1983
BstUI CGCG 2 cut(s) 425, 729
BstV1I GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1441, 1463, 1738, 2326
BstX2I RGATCY 3 cut(s) 1958, 2149, 2286
BstXI CCANNNNNNTGG 1 cut(s) 1186
BstYI RGATCY 3 cut(s) 1958, 2149, 2286
BstZ17I GTATAC 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 975
BsuRI GGCC 5 cut(s) 1345, 1436, 1606, 2231, 2427
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 2218
BtsCI GGATG 4 cut(s) 307, 643, 1129, 2275
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 5 cut(s) 1114, 1381, 1533, 2318, 2319
BveI ACCTGC 1 cut(s) 1224
Cac8I GCNNGC 6 cut(s) 51, 282, 1347, 1434, 2060, 2415
CaiI CAGNNNCTG 2 cut(s) 115, 625
CciI TCATGA 2 cut(s) 1222, 2022
CfoI GCGC 2 cut(s) 427, 731
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 2 cut(s) 254, 1008
Csp6I GTAC 2 cut(s) 1182, 1535
CviAII CATG 8 cut(s) 269, 290, 434, 550, 1223, 1333, 1849, 2023
CviQI GTAC 2 cut(s) 1182, 1535
Eam1104I CTCTTC 3 cut(s) 1429, 2043, 2217
EarI CTCTTC 3 cut(s) 1429, 2043, 2217
EciI GGCGGA 2 cut(s) 215, 257
Eco130I CCWWGG 2 cut(s) 475, 549
Eco147I AGGCCT 2 cut(s) 1606, 2427
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 2 cut(s) 1014, 1064
Eco32I GATATC 2 cut(s) 195, 875
Eco47I GGWCC 3 cut(s) 97, 1565, 1583
Eco57I CTGAAG 3 cut(s) 92, 1482, 2276
EcoRI GAATTC 2 cut(s) 802, 840
EcoRII CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
EcoRV GATATC 2 cut(s) 195, 875
EcoT14I CCWWGG 2 cut(s) 475, 549
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 2 cut(s) 475, 549
FaeI CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2026
FalI AAGNNNNNCTT 4 cut(s) 483, 515, 696, 728
FaqI GGGAC 6 cut(s) 493, 664, 1018, 1103, 1478, 1551
FatI CATG 8 cut(s) 268, 289, 433, 549, 1222, 1332, 1848, 2022
FauI CCCGC 3 cut(s) 479, 1915, 2001
FauNDI CATATG 1 cut(s) 529
FblI GTMKAC 2 cut(s) 518, 1238
FokI GGATG 4 cut(s) 314, 650, 1136, 2282
FriOI GRGCYC 1 cut(s) 471
FspBI CTAG 3 cut(s) 215, 1139, 1532
GlaI GCGC 2 cut(s) 426, 730
GsaI CCCAGC 1 cut(s) 1329
HaeIII GGCC 5 cut(s) 1345, 1436, 1606, 2231, 2427
HapII CCGG 3 cut(s) 153, 252, 1428
HgaI GACGC 2 cut(s) 254, 1008
HhaI GCGC 2 cut(s) 427, 731
Hin1II CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2026
Hin6I GCGC 2 cut(s) 425, 729
HinP1I GCGC 2 cut(s) 425, 729
HincII GTYRAC 2 cut(s) 1239, 2325
HindII GTYRAC 2 cut(s) 1239, 2325
HindIII AAGCTT 2 cut(s) 104, 2072
HpaI GTTAAC 1 cut(s) 2325
HpaII CCGG 3 cut(s) 153, 252, 1428
HphI GGTGA 5 cut(s) 142, 499, 902, 1477, 2192
Hpy166II GTNNAC 6 cut(s) 212, 519, 910, 1239, 1650, 2325
Hpy8I GTNNAC 6 cut(s) 212, 519, 910, 1239, 1650, 2325
Hpy99I CGWCG 3 cut(s) 11, 89, 92
HpyCH4III ACNGT 9 cut(s) 148, 1029, 1186, 1258, 1376, 1528, 1565, 2314, 2322
HpyCH4IV ACGT 5 cut(s) 84, 961, 1491, 2190, 2327
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
HpySE526I ACGT 5 cut(s) 84, 961, 1491, 2190, 2327
Hsp92II CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2026
HspAI GCGC 2 cut(s) 425, 729
KspAI GTTAAC 1 cut(s) 2325
LguI GCTCTTC 1 cut(s) 2217
LmnI GCTCC 4 cut(s) 112, 466, 641, 2392
Lsp1109I GCAGC 9 cut(s) 102, 550, 612, 925, 1281, 1441, 1463, 1738, 2326
LweI GCATC 7 cut(s) 72, 464, 918, 925, 940, 1006, 1801
MaeI CTAG 3 cut(s) 215, 1139, 1532
MaeII ACGT 5 cut(s) 84, 961, 1491, 2190, 2327
MaeIII GTNAC 5 cut(s) 148, 541, 895, 1262, 2314
MbiI CCGCTC 1 cut(s) 2055
MfeI CAATTG 3 cut(s) 179, 512, 780
MflI RGATCY 3 cut(s) 1958, 2149, 2286
MhlI GDGCHC 1 cut(s) 471
MlyI GAGTC 5 cut(s) 1085, 1253, 1451, 1718, 2398
MmeI TCCRAC 1 cut(s) 444
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
MslI CAYNNNNRTG 1 cut(s) 601
MspA1I CMGCKG 2 cut(s) 1349, 1454
MspI CCGG 3 cut(s) 153, 252, 1428
MspR9I CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
MunI CAATTG 3 cut(s) 179, 512, 780
Mva1269I GAATGC 2 cut(s) 2018, 2104
MvaI CCWGG 6 cut(s) 239, 1370, 1624, 1956, 2223, 2429
MvnI CGCG 2 cut(s) 425, 729
MwoI GCNNNNNNNGC 7 cut(s) 22, 50, 112, 419, 533, 625, 2216
NciI CCSGG 1 cut(s) 1429
NcoI CCATGG 1 cut(s) 549
NdeI CATATG 1 cut(s) 529
NlaIII CATG 8 cut(s) 272, 293, 437, 553, 1226, 1336, 1852, 2026
NlaIV GGNNCC 2 cut(s) 468, 1567
NmuCI GTSAC 3 cut(s) 148, 1262, 2314
NspI RCATGY 3 cut(s) 293, 437, 1852
PagI TCATGA 2 cut(s) 1222, 2022
PceI AGGCCT 2 cut(s) 1606, 2427
PciI ACATGT 1 cut(s) 289
PciSI GCTCTTC 1 cut(s) 2217
PctI GAATGC 2 cut(s) 2018, 2104
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 8 cut(s) 322, 356, 776, 785, 977, 1576, 1904, 2273
PflFI GACNNNGTC 1 cut(s) 1243
PfoI TCCNGGA 2 cut(s) 1622, 1954
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 5 cut(s) 1085, 1252, 1450, 1717, 2398
PpsI GAGTC 5 cut(s) 1085, 1252, 1450, 1717, 2398
PscI ACATGT 1 cut(s) 289
Psp1406I AACGTT 1 cut(s) 2327
Psp6I CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 6 cut(s) 237, 1368, 1622, 1954, 2221, 2427
PspN4I GGNNCC 2 cut(s) 468, 1567
PspPI GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
PsrI GAACNNNNNNTAC 2 cut(s) 1795, 1827
PstI CTGCAG 1 cut(s) 27
PstNI CAGNNNCTG 2 cut(s) 115, 625
PsuI RGATCY 3 cut(s) 1958, 2149, 2286
PsyI GACNNNGTC 1 cut(s) 1243
PvuI CGATCG 1 cut(s) 79
PvuII CAGCTG 2 cut(s) 1349, 1454
RsaI GTAC 2 cut(s) 1183, 1536
RsaNI GTAC 2 cut(s) 1182, 1535
RseI CAYNNNNRTG 1 cut(s) 601
SalI GTCGAC 1 cut(s) 1237
SapI GCTCTTC 1 cut(s) 2217
SaqAI TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
Sau96I GGNCC 5 cut(s) 97, 1343, 1565, 1583, 2229
ScaI AGTACT 1 cut(s) 1183
SchI GAGTC 5 cut(s) 1085, 1253, 1451, 1718, 2398
ScrFI CCNGG 7 cut(s) 239, 1370, 1429, 1624, 1956, 2223, 2429
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 7 cut(s) 72, 464, 918, 925, 940, 1006, 1801
SfcI CTRYAG 3 cut(s) 23, 657, 1983
SinI GGWCC 3 cut(s) 97, 1565, 1583
SmiMI CAYNNNNRTG 1 cut(s) 601
SmlI CTYRAG 3 cut(s) 1011, 1061, 2130
SmoI CTYRAG 3 cut(s) 1011, 1061, 2130
SseBI AGGCCT 2 cut(s) 1606, 2427
SsiI CCGC 8 cut(s) 200, 242, 423, 472, 1661, 1922, 2008, 2055
SspI AATATT 1 cut(s) 1083
SspMI CTAG 3 cut(s) 215, 1139, 1532
StuI AGGCCT 2 cut(s) 1606, 2427
StyD4I CCNGG 7 cut(s) 237, 1368, 1427, 1622, 1954, 2221, 2427
StyI CCWWGG 2 cut(s) 475, 549
TaaI ACNGT 9 cut(s) 148, 1029, 1186, 1258, 1376, 1528, 1565, 2314, 2322
TaiI ACGT 5 cut(s) 87, 964, 1494, 2193, 2330
TaqI TCGA 9 cut(s) 36, 59, 79, 90, 126, 132, 957, 975, 1238
TatI WGTACW 1 cut(s) 1181
TauI GCSGC 2 cut(s) 425, 2058
TfiI GAWTC 8 cut(s) 322, 356, 776, 785, 977, 1576, 1904, 2273
Tru1I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
Tru9I TTAA 7 cut(s) 687, 813, 1613, 1718, 1799, 2324, 2330
TscAI CASTG 5 cut(s) 1114, 1381, 1533, 2319, 2325
TseFI GTSAC 3 cut(s) 148, 1262, 2314
TseI GCWGC 9 cut(s) 115, 563, 625, 938, 1269, 1451, 1454, 1726, 2339
Tsp45I GTSAC 3 cut(s) 148, 1262, 2314
TspGWI ACGGA 2 cut(s) 959, 1304
TspRI CASTG 5 cut(s) 1114, 1381, 1533, 2319, 2325
Tth111I GACNNNGTC 1 cut(s) 1243
VpaK11BI GGWCC 3 cut(s) 97, 1565, 1583
XapI RAATTY 4 cut(s) 802, 840, 1896, 1948
XbaI TCTAGA 1 cut(s) 1138
XceI RCATGY 3 cut(s) 293, 437, 1852
XcmI CCANNNNNNNNNTGG 1 cut(s) 1577
XmiI GTMKAC 2 cut(s) 518, 1238
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 3 cut(s) 215, 1139, 1532
ZrmI AGTACT 1 cut(s) 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.