Rw5G038490

E3 UFM1-protein ligase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007320
Physical Location & Seq
Reverse (-)
67811200 .. 67819265
8066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038490.1

Sequence Viewer

Length: 2457 bp
ATGGACGACGAATTGGTGGAATTGCAGAAGCAATTCGAGTCGGCACAGCAAGCGAAATCGAGCATCAGATTGTCCGATCGAAACGTCGTCGAATTGGTCCAAAAGCTTCACGAGCTCCACATCATCGACTTCGACCTCCTCCACACCGTCTCCGGCAAAGAATACATCACTCCGGATCAATTGAGGCATGAAATACTGGTGGAGGTCAAGAGGTTGGGCCGTATATCACTGATTGACCTTGCAGATGCTATTGGGGTTGATTTGTACCATGTCGAGAAGCAAGCTCAACATGTGGTTTCTGATGATCCGGGGCTTATGTTGATTCAGGGGGAAATAATATCACAGTCCTATTGGGATTCCGTCGCTGAAGAAATCAATGAGAGGCTTCAAGAATGTAGCCAAGTTGCTTTAGCAGAACTTGCCGCGCAGTTGCATGTCAGTTCTGAAATGGTGACATCTGTGTTGGAGCCGCGCCTTGGGACTATGGTGAAAGGTAGGCTTGAAGGTGGGCAATTGTATACCCCTGCTTATGTTGCACGTGTCACTGCCATGGTTCGTGGTGCTGCTAGGGCTATCACAGTTCCCACAAACTTATCAGTCTTGTGGAGTTCTTTACAGCAGTTGCTGCAAGAAATGGAGGGAGCCAGTGGTGTAGCTGTAGAAGGTTCATTTTTCCAATCCCTTTTTAACGGGCTTGTAAAGGAAGGCGAGATTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCAAATGTTTTTGCCATTGCTCAGAAGGAATCAATTGATTCTTTCTTCTCACAGAACTCTTTCATTAGCTATGACGTTCTGCAGAAGCTTAGAATTCCCCAGCCTGATCAGTTCTTGCAGTCCAGATATCCTGAATGCATACCTTTGGTTACTACATTTATTCACCCATCAATGATTGAGATGCTTGATGCTGCTATAGAAGATGCTCTTGAGCGTGGGAGCTGGATGGATTCTCTTTCTATACTGCCAATGTCCTTTGGATCTCAAGATGCATCCAAACTTTTGTCCCTTTGTCCTTCCATTCAGCAGGGCCTCAAGTCTGATAAGGCAATAATCTTCGGGGAATCCTTTGTATTTAGCAGTGCCTTTATCAAGGATGTGTATGATCACCTGGAGAAAGAAATGGAAACCTTTATAGTTTCAAGTACTTCTGGTACTATGGTGTCAGATGATTTGCAGGAGACAAAAGTTGGTCATGAAACAAGCAGGTTCACCGAATCAAATGAAATGGTTAGTGACAGCAGCAGTAATAAACAGACCATGGAGAAAGGATCAAAGAAGAAAAAGGGTCGAGTGGCTGGAAATATGGGAGCAGGTGCGGCCGAAGGTGATCCGGAAAACCAGGACTCTGTTCCTACAAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATTCCTCGTCCGCACAAGTTGCTGACTCAAAAGCATCTGCTAAGTTGGTAAAATCTAAAGAGGAAAATCTGAACATTCCTTCAGAAGATTGGATGATGAACAAGATTGCAACACTGGTTCCTGAATTCGAAGAACAAGGTCTTGATGATCCACAAACAATTATTAGACCTTTGGCAAACCATGTGAGACCTATGCTAATAAACTCTTGGAAGGAGAGAAGAAAGGCATTGTTTACTGAAAATGCAGAGAGAATGAAGCACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAACATGCAGCTCTACGAAAAAGCTTTAGATTTGTTTGAAGATGACCCATCAACTTCAGTCATTTTGCACCGGCATTTGTTAAGAACAACAGCTAGTACTATTGTGGATATGCTTCTTCTTAATTTGGACATGCTCAACAAATTGAAGAATGGAGTTGAAGTTGAGGACACTCAGATTTCAGAATCTTCTTCACTTAACCCAGGAGAAAGAACTTCCATTGCTAAGAATTTTCCTGGATCTCTCTCAAAGAAGGCTCTTGTTGTTGTTGAAGCTTTGGAAGGAAAGCGGGTGGAAACATTCATGACTGCTTTAAGAGACATAGCTGAAGAAAGTGGTTTACTCCTGAAAAAGCTTGACAAGAAGTTGGAAAGAACACTTCTGCATTCGTATCAAAAGGATTTGGCGTCTCAAGTTTCTGCTGAAATGGATCCAGTCTCTGTTTTGGCCAAAGTTGTTTCCCTGCTATATGTACAGATTCACCATAAAGCTCTCCAAGCACCTGGAAGGGCCATCGCTGTTGCTGTTTCTCGGCTGAAGGATAAACTAGATGAATCAGCTTTCAAGATCCTGACAGAATATCAAACTGCAACAGTAACCCTCTTAGCACTAATATCCGCTGCAAGTGGAGATGAAGAAGACTGTACATCTGATAGAATATTGAGCAAAAGGGAGCTCCTGGAAAATCAAATGCCTGCACTGAAAGGCCTGGTTTTGCGCACTGGCACCTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
E3_UFM1_ligase

Protein Analysis

818

Amino Acids

90.76

Weight (kDa)

5.3

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 4 - 288 5.6e-100 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 305 - 352 1.3e-09 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 561 - 691 3e-38 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 696 - 809 1.4e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1331
Acc16I TGCGCA 1 cut(s) 2435
Acc36I ACCTGC 2 cut(s) 1224, 1331
AccB1I GGYRCC 1 cut(s) 2441
AccI GTMKAC 1 cut(s) 518
AccII CGCG 2 cut(s) 425, 472
AccIII TCCGGA 2 cut(s) 172, 1360
AciI CCGC 6 cut(s) 423, 470, 1346, 1428, 2005, 2334
AcoI YGGCCR 2 cut(s) 1347, 2163
AcsI RAATTY 4 cut(s) 840, 1417, 1541, 1945
AcuI CTGAAG 5 cut(s) 387, 1482, 1758, 2064, 2273
AcvI CACGTG 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 2123
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 6 cut(s) 266, 1174, 1183, 1816, 2190, 2362
AfiI CCNNNNNNNGG 1 cut(s) 476
AflIII ACRYGT 2 cut(s) 289, 538
AgsI TTSAA 8 cut(s) 389, 503, 1170, 1757, 1864, 1877, 1988, 2281
AjnI CCWGG 7 cut(s) 1137, 1368, 1918, 1951, 2218, 2394, 2424
Alw21I GWGCWC 2 cut(s) 117, 2394
Alw26I GTCTC 6 cut(s) 154, 1202, 1597, 2028, 2130, 2158
AlwNI CAGNNNCTG 2 cut(s) 625, 2156
Aor13HI TCCGGA 2 cut(s) 172, 1360
AoxI GGCC 6 cut(s) 217, 1057, 1347, 2163, 2226, 2422
ApeKI GCWGC 6 cut(s) 563, 625, 938, 1269, 1726, 2336
ApoI RAATTY 4 cut(s) 840, 1417, 1541, 1945
Asp700I GAANNNNTTC 2 cut(s) 32, 806
AspLEI GCGC 3 cut(s) 427, 474, 2436
AspS9I GGNCC 4 cut(s) 97, 217, 1057, 2226
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 7 cut(s) 463, 499, 902, 1127, 1231, 1367, 2189
AsuII TTCGAA 1 cut(s) 1545
AvaII GGWCC 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 1173, 1206
BalI TGGCCA 1 cut(s) 2165
BamHI GGATCC 1 cut(s) 2146
BanI GGYRCC 1 cut(s) 2441
BanII GRGCYC 2 cut(s) 117, 2394
BauI CACGAG 1 cut(s) 110
BbrPI CACGTG 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 2361
Bbv12I GWGCWC 2 cut(s) 117, 2394
BbvI GCAGC 6 cut(s) 550, 612, 925, 1281, 1738, 2323
BccI CCATC 4 cut(s) 922, 967, 1774, 2237
BceAI ACGGC 1 cut(s) 204
BciT130I CCWGG 7 cut(s) 1139, 1370, 1920, 1953, 2220, 2396, 2426
BclI TGATCA 2 cut(s) 853, 1132
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 6 cut(s) 154, 1202, 1597, 2028, 2130, 2158
BfaI CTAG 3 cut(s) 567, 1812, 2264
BfmI CTRYAG 3 cut(s) 657, 827, 942
BfuAI ACCTGC 2 cut(s) 1224, 1331
BisI GCNGC 9 cut(s) 423, 470, 564, 626, 939, 1270, 1347, 1727, 2337
BlsI GCNGC 9 cut(s) 424, 471, 565, 627, 940, 1271, 1348, 1728, 2338
BmcAI AGTACT 2 cut(s) 1174, 1816
Bme1390I CCNGG 8 cut(s) 309, 1139, 1370, 1920, 1953, 2220, 2396, 2426
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 97, 217, 1057, 2226
BmiI GGNNCC 5 cut(s) 468, 643, 1536, 2148, 2443
BmrFI CCNGG 8 cut(s) 309, 1139, 1370, 1920, 1953, 2220, 2396, 2426
BmsI GCATC 8 cut(s) 72, 235, 918, 925, 940, 1006, 1028, 1460
BpiI GAAGAC 1 cut(s) 2361
BpmI CTGGAG 1 cut(s) 1160
Bpu14I TTCGAA 1 cut(s) 1545
BpuEI CTTGAG 4 cut(s) 977, 996, 1046, 2112
BpuMI CCSGG 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 539
BsaHI GRCGYC 1 cut(s) 2123
BsaI GGTCTC 1 cut(s) 1597
BsaJI CCNNGG 5 cut(s) 308, 475, 549, 1287, 1918
BsaWI WCCGGW 2 cut(s) 172, 1360
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1863, 1893
Bsc4I CCNNNNNNNGG 1 cut(s) 476
Bse118I RCCGGY 1 cut(s) 1788
Bse1I ACTGG 5 cut(s) 201, 645, 1536, 2150, 2443
Bse3DI GCAATG 2 cut(s) 762, 1935
BseAI TCCGGA 2 cut(s) 172, 1360
BseBI CCWGG 7 cut(s) 1139, 1370, 1920, 1953, 2220, 2396, 2426
BseDI CCNNGG 5 cut(s) 308, 475, 549, 1287, 1918
BseGI GGATG 4 cut(s) 978, 1019, 1129, 1515
BseLI CCNNNNNNNGG 1 cut(s) 476
BseMI GCAATG 2 cut(s) 762, 1935
BseMII CTCAG 2 cut(s) 782, 1904
BseNI ACTGG 5 cut(s) 201, 645, 1536, 2150, 2443
BseRI GAGGAG 1 cut(s) 128
BseX3I CGGCCG 1 cut(s) 1347
BseXI GCAGC 6 cut(s) 550, 612, 925, 1281, 1738, 2323
BseYI CCCAGC 1 cut(s) 846
BsgI GTGCAG 2 cut(s) 750, 2397
Bsh1236I CGCG 2 cut(s) 425, 472
Bsh1285I CGRYCG 2 cut(s) 79, 1350
BshFI GGCC 6 cut(s) 219, 1059, 1349, 2165, 2228, 2424
BshNI GGYRCC 1 cut(s) 2441
BsiEI CGRYCG 2 cut(s) 79, 1350
BsiHKAI GWGCWC 2 cut(s) 117, 2394
BsiSI CCGG 5 cut(s) 153, 173, 308, 1361, 1789
BslFI GGGAC 2 cut(s) 493, 1018
BslI CCNNNNNNNGG 1 cut(s) 476
BsmAI GTCTC 6 cut(s) 154, 1202, 1597, 2028, 2130, 2158
BsmBI CGTCTC 2 cut(s) 154, 2130
BsmFI GGGAC 2 cut(s) 493, 1018
BsmI GAATGC 2 cut(s) 887, 2101
BsnI GGCC 6 cut(s) 219, 1059, 1349, 2165, 2228, 2424
Bso31I GGTCTC 1 cut(s) 1597
Bsp119I TTCGAA 1 cut(s) 1545
Bsp1286I GDGCHC 2 cut(s) 117, 2394
Bsp13I TCCGGA 2 cut(s) 172, 1360
Bsp1407I TGTACA 2 cut(s) 2188, 2360
Bsp19I CCATGG 2 cut(s) 549, 1287
BspACI CCGC 6 cut(s) 423, 470, 1346, 1428, 2005, 2334
BspANI GGCC 6 cut(s) 219, 1059, 1349, 2165, 2228, 2424
BspCNI CTCAG 2 cut(s) 781, 1903
BspEI TCCGGA 2 cut(s) 172, 1360
BspFNI CGCG 2 cut(s) 425, 472
BspHI TCATGA 2 cut(s) 1222, 2019
BspLI GGNNCC 5 cut(s) 468, 643, 1536, 2148, 2443
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 2 cut(s) 1224, 1331
BspT104I TTCGAA 1 cut(s) 1545
BspT107I GGYRCC 1 cut(s) 2441
BspTNI GGTCTC 1 cut(s) 1597
BsrDI GCAATG 2 cut(s) 762, 1935
BsrFI RCCGGY 1 cut(s) 1788
BsrGI TGTACA 2 cut(s) 2188, 2360
BsrI ACTGG 5 cut(s) 201, 645, 1536, 2150, 2443
BssAI RCCGGY 1 cut(s) 1788
BssECI CCNNGG 5 cut(s) 308, 475, 549, 1287, 1918
BssNAI GTATAC 1 cut(s) 519
BssNI GRCGYC 1 cut(s) 2123
BssSI CACGAG 1 cut(s) 110
BssT1I CCWWGG 3 cut(s) 475, 549, 1287
Bst1107I GTATAC 1 cut(s) 519
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 7 cut(s) 1139, 1370, 1920, 1953, 2220, 2396, 2426
Bst4CI ACNGT 5 cut(s) 148, 345, 580, 2311, 2360
BstACI GRCGYC 1 cut(s) 2123
BstAPI GCANNNNNTGC 3 cut(s) 419, 625, 1436
BstAUI TGTACA 2 cut(s) 2188, 2360
BstBAI YACGTR 1 cut(s) 539
BstBI TTCGAA 1 cut(s) 1545
BstC8I GCNNGC 3 cut(s) 51, 282, 2412
BstDEI CTNAG 7 cut(s) 768, 836, 1392, 1458, 1890, 1941, 2320
BstDSI CCRYGG 2 cut(s) 549, 1287
BstF5I GGATG 4 cut(s) 978, 1019, 1129, 1515
BstFNI CGCG 2 cut(s) 425, 472
BstHHI GCGC 3 cut(s) 427, 474, 2436
BstMAI GTCTC 6 cut(s) 154, 1202, 1597, 2028, 2130, 2158
BstMCI CGRYCG 2 cut(s) 79, 1350
BstMWI GCNNNNNNNGC 9 cut(s) 50, 112, 419, 533, 569, 625, 1346, 1436, 2213
BstNI CCWGG 7 cut(s) 1139, 1370, 1920, 1953, 2220, 2396, 2426
BstNSI RCATGY 4 cut(s) 293, 437, 1726, 1852
BstSCI CCNGG 8 cut(s) 307, 1137, 1368, 1918, 1951, 2218, 2394, 2424
BstSFI CTRYAG 3 cut(s) 657, 827, 942
BstUI CGCG 2 cut(s) 425, 472
BstV1I GCAGC 6 cut(s) 550, 612, 925, 1281, 1738, 2323
BstV2I GAAGAC 1 cut(s) 2361
BstX2I RGATCY 4 cut(s) 1007, 1955, 2146, 2283
BstXI CCANNNNNNTGG 1 cut(s) 2219
BstYI RGATCY 4 cut(s) 1007, 1955, 2146, 2283
BstZ17I GTATAC 1 cut(s) 519
BstZI CGGCCG 1 cut(s) 1347
BsuRI GGCC 6 cut(s) 219, 1059, 1349, 2165, 2228, 2424
BtgI CCRYGG 2 cut(s) 549, 1287
BtgZI GCGATG 1 cut(s) 2215
BtsCI GGATG 4 cut(s) 978, 1019, 1129, 1515
BtsI GCAGTG 2 cut(s) 543, 1114
BtsIMutI CAGTG 7 cut(s) 227, 543, 652, 1114, 1529, 2414, 2436
BveI ACCTGC 2 cut(s) 1224, 1331
Cac8I GCNNGC 3 cut(s) 51, 282, 2412
CaiI CAGNNNCTG 2 cut(s) 625, 2156
CciI TCATGA 2 cut(s) 1222, 2019
CfoI GCGC 3 cut(s) 427, 474, 2436
Cfr10I RCCGGY 1 cut(s) 1788
Cfr13I GGNCC 4 cut(s) 97, 217, 1057, 2226
CseI GACGC 1 cut(s) 2112
Csp6I GTAC 6 cut(s) 265, 1173, 1182, 1815, 2189, 2361
CviQI GTAC 6 cut(s) 265, 1173, 1182, 1815, 2189, 2361
DdeI CTNAG 7 cut(s) 768, 836, 1392, 1458, 1890, 1941, 2320
DraIII CACNNNGTG 1 cut(s) 728
EaeI YGGCCR 2 cut(s) 1347, 2163
EagI CGGCCG 1 cut(s) 1347
Ecl136II GAGCTC 2 cut(s) 115, 2392
EclXI CGGCCG 1 cut(s) 1347
Eco130I CCWWGG 3 cut(s) 475, 549, 1287
Eco147I AGGCCT 1 cut(s) 2424
Eco24I GRGCYC 2 cut(s) 117, 2394
Eco31I GGTCTC 1 cut(s) 1597
Eco32I GATATC 1 cut(s) 875
Eco47I GGWCC 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 1347
Eco53kI GAGCTC 2 cut(s) 115, 2392
Eco57I CTGAAG 5 cut(s) 387, 1482, 1758, 2064, 2273
Eco72I CACGTG 1 cut(s) 539
EcoICRI GAGCTC 2 cut(s) 115, 2392
EcoO109I RGGNCCY 1 cut(s) 1057
EcoRI GAATTC 3 cut(s) 840, 1417, 1541
EcoRII CCWGG 7 cut(s) 1137, 1368, 1918, 1951, 2218, 2394, 2424
EcoRV GATATC 1 cut(s) 875
EcoT14I CCWWGG 3 cut(s) 475, 549, 1287
EcoT22I ATGCAT 2 cut(s) 887, 1021
EcoT38I GRGCYC 2 cut(s) 117, 2394
ErhI CCWWGG 3 cut(s) 475, 549, 1287
Esp3I CGTCTC 2 cut(s) 154, 2130
FalI AAGNNNNNCTT 4 cut(s) 483, 515, 939, 971
FaqI GGGAC 2 cut(s) 493, 1018
FauI CCCGC 1 cut(s) 1998
FbaI TGATCA 2 cut(s) 853, 1132
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 9 cut(s) 423, 470, 564, 626, 939, 1270, 1347, 1727, 2337
FokI GGATG 4 cut(s) 985, 1006, 1136, 1522
FriOI GRGCYC 2 cut(s) 117, 2394
Fsp4HI GCNGC 9 cut(s) 423, 470, 564, 626, 939, 1270, 1347, 1727, 2337
FspBI CTAG 3 cut(s) 567, 1812, 2264
FspI TGCGCA 1 cut(s) 2435
GlaI GCGC 3 cut(s) 426, 473, 2435
GluI GCNGC 9 cut(s) 423, 470, 564, 626, 939, 1270, 1347, 1727, 2337
GsaI CCCAGC 1 cut(s) 850
GsuI CTGGAG 1 cut(s) 1160
HaeIII GGCC 6 cut(s) 219, 1059, 1349, 2165, 2228, 2424
HapII CCGG 5 cut(s) 153, 173, 308, 1361, 1789
HgaI GACGC 1 cut(s) 2112
HhaI GCGC 3 cut(s) 427, 474, 2436
Hin1I GRCGYC 1 cut(s) 2123
Hin6I GCGC 3 cut(s) 425, 472, 2434
HinP1I GCGC 3 cut(s) 425, 472, 2434
HindIII AAGCTT 5 cut(s) 104, 833, 1740, 1989, 2069
HpaII CCGG 5 cut(s) 153, 173, 308, 1361, 1789
HphI GGTGA 7 cut(s) 463, 499, 902, 1127, 1231, 1367, 2189
Hpy166II GTNNAC 4 cut(s) 519, 1239, 1650, 2057
Hpy8I GTNNAC 4 cut(s) 519, 1239, 1650, 2057
Hpy99I CGWCG 4 cut(s) 11, 89, 92, 365
HpyCH4III ACNGT 5 cut(s) 148, 345, 580, 2311, 2360
HpyCH4IV ACGT 3 cut(s) 84, 538, 822
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 112, 419, 533, 569, 625, 1346, 1436, 2213
HpyF3I CTNAG 7 cut(s) 768, 836, 1392, 1458, 1890, 1941, 2320
HpySE526I ACGT 3 cut(s) 84, 538, 822
Hsp92I GRCGYC 1 cut(s) 2123
HspAI GCGC 3 cut(s) 425, 472, 2434
Kpn2I TCCGGA 2 cut(s) 172, 1360
Ksp22I TGATCA 2 cut(s) 853, 1132
LmnI GCTCC 7 cut(s) 120, 466, 641, 966, 1337, 2389, 2397
Lsp1109I GCAGC 6 cut(s) 550, 612, 925, 1281, 1738, 2323
LweI GCATC 8 cut(s) 72, 235, 918, 925, 940, 1006, 1028, 1460
MaeI CTAG 3 cut(s) 567, 1812, 2264
MaeII ACGT 3 cut(s) 84, 538, 822
MaeIII GTNAC 5 cut(s) 451, 541, 895, 1262, 2311
MfeI CAATTG 3 cut(s) 179, 512, 780
MflI RGATCY 4 cut(s) 1007, 1955, 2146, 2283
MhlI GDGCHC 2 cut(s) 117, 2394
MlsI TGGCCA 1 cut(s) 2165
MluNI TGGCCA 1 cut(s) 2165
MlyI GAGTC 4 cut(s) 47, 1367, 1436, 1718
MmeI TCCRAC 2 cut(s) 444, 2064
Mox20I TGGCCA 1 cut(s) 2165
Mph1103I ATGCAT 2 cut(s) 887, 1021
MroI TCCGGA 2 cut(s) 172, 1360
MroXI GAANNNNTTC 2 cut(s) 32, 806
MscI TGGCCA 1 cut(s) 2165
MseI TTAA 6 cut(s) 687, 1718, 1799, 1839, 1914, 2030
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 2165
MspA1I CMGCKG 1 cut(s) 2336
MspI CCGG 5 cut(s) 153, 173, 308, 1361, 1789
MspR9I CCNGG 8 cut(s) 309, 1139, 1370, 1920, 1953, 2220, 2396, 2426
MunI CAATTG 3 cut(s) 179, 512, 780
Mva1269I GAATGC 2 cut(s) 887, 2101
MvaI CCWGG 7 cut(s) 1139, 1370, 1920, 1953, 2220, 2396, 2426
MvnI CGCG 2 cut(s) 425, 472
MwoI GCNNNNNNNGC 9 cut(s) 50, 112, 419, 533, 569, 625, 1346, 1436, 2213
NciI CCSGG 1 cut(s) 309
NcoI CCATGG 2 cut(s) 549, 1287
NlaIV GGNNCC 5 cut(s) 468, 643, 1536, 2148, 2443
NmeAIII GCCGAG 1 cut(s) 2227
NmuCI GTSAC 3 cut(s) 451, 541, 1262
NsbI TGCGCA 1 cut(s) 2435
NsiI ATGCAT 2 cut(s) 887, 1021
NspI RCATGY 4 cut(s) 293, 437, 1726, 1852
NspV TTCGAA 1 cut(s) 1545
PagI TCATGA 2 cut(s) 1222, 2019
PaqCI CACCTGC 1 cut(s) 1331
PceI AGGCCT 1 cut(s) 2424
PciI ACATGT 1 cut(s) 289
PctI GAATGC 2 cut(s) 887, 2101
PdmI GAANNNNTTC 2 cut(s) 32, 806
PfoI TCCNGGA 2 cut(s) 1951, 2394
PkrI GCNGC 9 cut(s) 424, 471, 565, 627, 940, 1271, 1348, 1728, 2338
Ple19I CGATCG 1 cut(s) 79
PleI GAGTC 4 cut(s) 46, 1367, 1436, 1717
PmaCI CACGTG 1 cut(s) 539
PmlI CACGTG 1 cut(s) 539
PpsI GAGTC 4 cut(s) 46, 1367, 1436, 1717
Ppu21I YACGTR 1 cut(s) 539
PscI ACATGT 1 cut(s) 289
Psp124BI GAGCTC 2 cut(s) 117, 2394
Psp6I CCWGG 7 cut(s) 1137, 1368, 1918, 1951, 2218, 2394, 2424
PspCI CACGTG 1 cut(s) 539
PspFI CCCAGC 1 cut(s) 846
PspGI CCWGG 7 cut(s) 1137, 1368, 1918, 1951, 2218, 2394, 2424
PspN4I GGNNCC 5 cut(s) 468, 643, 1536, 2148, 2443
PspPI GGNCC 4 cut(s) 97, 217, 1057, 2226
PstI CTGCAG 1 cut(s) 831
PstNI CAGNNNCTG 2 cut(s) 625, 2156
PsuI RGATCY 4 cut(s) 1007, 1955, 2146, 2283
PvuI CGATCG 1 cut(s) 79
RsaI GTAC 6 cut(s) 266, 1174, 1183, 1816, 2190, 2362
RsaNI GTAC 6 cut(s) 265, 1173, 1182, 1815, 2189, 2361
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 2 cut(s) 117, 2394
SaqAI TTAA 6 cut(s) 687, 1718, 1799, 1839, 1914, 2030
SatI GCNGC 9 cut(s) 423, 470, 564, 626, 939, 1270, 1347, 1727, 2337
Sau96I GGNCC 4 cut(s) 97, 217, 1057, 2226
ScaI AGTACT 2 cut(s) 1174, 1816
SchI GAGTC 4 cut(s) 47, 1367, 1436, 1718
ScrFI CCNGG 8 cut(s) 309, 1139, 1370, 1920, 1953, 2220, 2396, 2426
SduI GDGCHC 2 cut(s) 117, 2394
SfaNI GCATC 8 cut(s) 72, 235, 918, 925, 940, 1006, 1028, 1460
SfcI CTRYAG 3 cut(s) 657, 827, 942
SfuI TTCGAA 1 cut(s) 1545
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 4 cut(s) 956, 1011, 1061, 2127
SmoI CTYRAG 4 cut(s) 956, 1011, 1061, 2127
SseBI AGGCCT 1 cut(s) 2424
SsiI CCGC 6 cut(s) 423, 470, 1346, 1428, 2005, 2334
SspI AATATT 1 cut(s) 2376
SspMI CTAG 3 cut(s) 567, 1812, 2264
SstI GAGCTC 2 cut(s) 117, 2394
StuI AGGCCT 1 cut(s) 2424
StyD4I CCNGG 8 cut(s) 307, 1137, 1368, 1918, 1951, 2218, 2394, 2424
StyI CCWWGG 3 cut(s) 475, 549, 1287
TaaI ACNGT 5 cut(s) 148, 345, 580, 2311, 2360
TaiI ACGT 3 cut(s) 87, 541, 825
TaqI TCGA 9 cut(s) 36, 59, 79, 90, 126, 132, 273, 1318, 1545
TatI WGTACW 4 cut(s) 1172, 1814, 2188, 2360
TauI GCSGC 3 cut(s) 425, 472, 1349
Tru1I TTAA 6 cut(s) 687, 1718, 1799, 1839, 1914, 2030
Tru9I TTAA 6 cut(s) 687, 1718, 1799, 1839, 1914, 2030
TscAI CASTG 7 cut(s) 234, 550, 652, 1114, 1536, 2421, 2443
TseFI GTSAC 3 cut(s) 451, 541, 1262
TseI GCWGC 6 cut(s) 563, 625, 938, 1269, 1726, 2336
Tsp45I GTSAC 3 cut(s) 451, 541, 1262
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 7 cut(s) 234, 550, 652, 1114, 1536, 2421, 2443
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 4 cut(s) 840, 1417, 1541, 1945
XceI RCATGY 4 cut(s) 293, 437, 1726, 1852
XmiI GTMKAC 1 cut(s) 518
XmnI GAANNNNTTC 2 cut(s) 32, 806
XspI CTAG 3 cut(s) 567, 1812, 2264
ZrmI AGTACT 2 cut(s) 1174, 1816
Zsp2I ATGCAT 2 cut(s) 887, 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.