AT3G46630

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012457
Physical Location & Seq
Reverse (-)
17180678 .. 17182560
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G46630.1

Sequence Viewer

Length: 624 bp
ATGACCTCGCTACTTCTTCTCAGAACGCTTCCGCTTCTCCGCAACGGATTTCTCAACCATCGTCATCAAGTTGGTATCGTAGCTGGTGGAATTCTCAGTCACCGTCGGCGTTTGTTGTGTTCTCTTGCTGATAGACCACAGTTTTACGAGAATAATGATTCTGTTTCTCCGGTGGAGGGATCCGGCGCGGCGGCGATGAACGTGACATCGCCTGTTGTTGATAATAGCTGGCGTTATGAAGATCCTGATTATCGGAAATGGAAGAATCTAGAGGCTGAGATTCTTCGAGATATTGAACCTATTTCTCTTCTCGCTAAAGAAATTCTTCATTCCGATAGGTATTTGGATGGAGAACGCTTAGATTTTGAGGATGAGAAAATTGTCATGGAAAAGCTTCTTCCTTATCACCCATATTCCAAAGACAAAATTGGTTGTGGTCTTGATTTTATAATGGTTGATCGGCATCCACAATTCAGGCACTCAAGATGCCTCTTTGTTGTGAGAACAGATGGTGGATGGATAGATTTCTCGTACCAAAAGTGTCTTCGAGCCTATGTTCGAGATAAGTACCCGTCTCACGCTGAGAGGTTTATTCGTGAACATTTTAAGCGTGCTAGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

207

Amino Acids

24.19

Weight (kDa)

8.32

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 106 - 182 1.1e-29 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccII CGCG 1 cut(s) 188
AciI CCGC 4 cut(s) 32, 40, 188, 191
AclWI GGATC 3 cut(s) 174, 187, 236
AcsI RAATTY 2 cut(s) 90, 321
AfaI GTAC 2 cut(s) 533, 569
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 1 cut(s) 296
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AluBI AGCT 4 cut(s) 83, 228, 394, 621
AluI AGCT 4 cut(s) 83, 228, 394, 621
Alw26I GTCTC 1 cut(s) 579
AlwI GGATC 3 cut(s) 174, 187, 236
ApoI RAATTY 2 cut(s) 90, 321
Asp700I GAANNNNTTC 2 cut(s) 324, 393
AspLEI GCGC 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 92, 398
BamHI GGATCC 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 536
BccI CCATC 4 cut(s) 66, 341, 503, 510
BcoDI GTCTC 1 cut(s) 579
BfaI CTAG 2 cut(s) 269, 615
BisI GCNGC 2 cut(s) 189, 192
BlsI GCNGC 2 cut(s) 190, 193
BmiI GGNNCC 1 cut(s) 181
BmsI GCATC 2 cut(s) 472, 476
BpiI GAAGAC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 462
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 462
BseGI GGATG 4 cut(s) 352, 376, 463, 521
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMII CTCAG 4 cut(s) 34, 109, 267, 573
Bsh1236I CGCG 1 cut(s) 188
BsiSI CCGG 2 cut(s) 170, 183
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 579
BsmBI CGTCTC 1 cut(s) 579
Bsp143I GATC 3 cut(s) 179, 241, 457
BspACI CCGC 4 cut(s) 32, 40, 188, 191
BspCNI CTCAG 4 cut(s) 33, 108, 268, 574
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 3 cut(s) 174, 187, 236
BssMI GATC 3 cut(s) 179, 241, 457
Bst4CI ACNGT 2 cut(s) 104, 141
Bst6I CTCTTC 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 230, 612
BstDEI CTNAG 5 cut(s) 20, 95, 276, 358, 582
BstF5I GGATG 4 cut(s) 352, 376, 463, 521
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 3 cut(s) 182, 244, 460
BstMAI GTCTC 1 cut(s) 579
BstMBI GATC 3 cut(s) 179, 241, 457
BstUI CGCG 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 2 cut(s) 179, 241
BstYI RGATCY 2 cut(s) 179, 241
BtgZI GCGATG 2 cut(s) 192, 209
BtsCI GGATG 4 cut(s) 352, 376, 463, 521
Cac8I GCNNGC 2 cut(s) 230, 612
CfoI GCGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 532, 568
CviAII CATG 1 cut(s) 385
CviJI RGCY 6 cut(s) 83, 228, 275, 394, 551, 621
CviKI_1 RGCY 6 cut(s) 83, 228, 275, 394, 551, 621
CviQI GTAC 2 cut(s) 532, 568
DdeI CTNAG 5 cut(s) 20, 95, 276, 358, 582
DpnI GATC 3 cut(s) 181, 243, 459
DpnII GATC 3 cut(s) 179, 241, 457
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 579
FaeI CATG 1 cut(s) 388
FaiI YATR 5 cut(s) 237, 386, 412, 449, 555
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 1 cut(s) 384
Fnu4HI GCNGC 2 cut(s) 189, 192
FokI GGATG 4 cut(s) 359, 383, 450, 528
Fsp4HI GCNGC 2 cut(s) 189, 192
FspBI CTAG 2 cut(s) 269, 615
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 189, 192
HapII CCGG 2 cut(s) 170, 183
HhaI GCGC 1 cut(s) 188
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 3 cut(s) 158, 265, 280
HpaII CCGG 2 cut(s) 170, 183
HphI GGTGA 2 cut(s) 92, 398
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 3 cut(s) 23, 255, 334
Hpy188III TCNNGA 7 cut(s) 245, 269, 287, 440, 483, 560, 596
Hpy8I GTNNAC 1 cut(s) 599
Hpy99I CGWCG 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 104, 141
HpyCH4IV ACGT 1 cut(s) 201
HpyF3I CTNAG 5 cut(s) 20, 95, 276, 358, 582
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 186
Kzo9I GATC 3 cut(s) 179, 241, 457
LpnPI CCDG 7 cut(s) 69, 183, 196, 214, 225, 258, 460
LweI GCATC 2 cut(s) 472, 476
MaeI CTAG 2 cut(s) 269, 615
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 98, 202
MalI GATC 3 cut(s) 181, 243, 459
MboI GATC 3 cut(s) 179, 241, 457
MboII GAAGA 8 cut(s) 8, 251, 274, 275, 299, 317, 389, 536
MflI RGATCY 2 cut(s) 179, 241
MluCI AATT 5 cut(s) 90, 321, 378, 426, 470
MnlI CCTC 6 cut(s) 16, 169, 265, 361, 500, 579
MroXI GAANNNNTTC 2 cut(s) 324, 393
MseI TTAA 1 cut(s) 606
MspI CCGG 2 cut(s) 170, 183
MvnI CGCG 1 cut(s) 188
NdeII GATC 3 cut(s) 179, 241, 457
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 98, 202
PdmI GAANNNNTTC 2 cut(s) 324, 393
PfeI GAWTC 3 cut(s) 158, 265, 280
PkrI GCNGC 2 cut(s) 190, 193
PsiI TTATAA 1 cut(s) 449
PspN4I GGNNCC 1 cut(s) 181
PsuI RGATCY 2 cut(s) 179, 241
RsaI GTAC 2 cut(s) 533, 569
RsaNI GTAC 2 cut(s) 532, 568
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 2 cut(s) 189, 192
Sau3AI GATC 3 cut(s) 179, 241, 457
SetI ASST 9 cut(s) 8, 85, 204, 230, 301, 341, 396, 590, 623
SfaNI GCATC 2 cut(s) 472, 476
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 5 cut(s) 90, 321, 378, 426, 470
SsiI CCGC 4 cut(s) 32, 40, 188, 191
SspMI CTAG 2 cut(s) 269, 615
TaaI ACNGT 2 cut(s) 104, 141
TaiI ACGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 286, 547, 559
TasI AATT 5 cut(s) 90, 321, 378, 426, 470
TauI GCSGC 2 cut(s) 191, 194
TfiI GAWTC 3 cut(s) 158, 265, 280
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TseFI GTSAC 2 cut(s) 98, 202
Tsp45I GTSAC 2 cut(s) 98, 202
TspDTI ATGAA 3 cut(s) 212, 252, 317
TspGWI ACGGA 1 cut(s) 60
XapI RAATTY 2 cut(s) 90, 321
XbaI TCTAGA 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 324, 393
XspI CTAG 2 cut(s) 269, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.