pycom08g07580

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012457
Physical Location & Seq
Reverse (-)
6113130 .. 6115566
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07580.1

Sequence Viewer

Length: 624 bp
ATGACGACGACGACGCGAATGGCGATTCTGAACCCGTTGCTGCTGCGTACAGTTTCGTTCCTCTGCGCGCGAGTCCACCACCGACGCCTGGGCCTCGGGCTTGCGGTCCGGAGCGGCAGGGCGTGGTGCAGCGGGGCGAGCGAGTCGACTCGGCCGGAGAGTCTATTGTCCTCGGTGGGCAGGTCTGCACTGAGAGGAGTGCTAAGCGTGAAGGAGCCACCGAAGTACCCTCGCTGGGATGACCCTGACTACTGCAAGTGGAAGGATAAGGAACGGGAGATTTTAAACGACATCGAGCCCGTCGTTTTGCTGGCCAAGGAGATTCTGCACTCTGATAGGTATATGGATGGTGAGCGGCTGACGGAGGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCACACTCAGAGGATAAAATTGGCTGCGCAATTGATTCTATCATGGTTGATCGGCATCCCCAATTTAAACGCTCGAGGTGCCTATTTGTCATAAGAACTGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTCCGAGCATATATCCGAGATAAGTACCCATCCCATGCAGATAGATTCATTCGGGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

208

Amino Acids

23.99

Weight (kDa)

9.18

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 106 - 182 4.2e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 171
AccB1I GGYRCC 1 cut(s) 486
AccBSI CCGCTC 2 cut(s) 114, 355
AccI GTMKAC 1 cut(s) 146
AccII CGCG 3 cut(s) 16, 68, 70
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 4 cut(s) 104, 114, 132, 355
AcoI YGGCCR 2 cut(s) 152, 312
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 3 cut(s) 49, 227, 569
AfiI CCNNNNNNNGG 2 cut(s) 88, 235
AjnI CCWGG 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 548
Ama87I CYCGRG 2 cut(s) 95, 481
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 3 cut(s) 91, 152, 312
ApeKI GCWGC 4 cut(s) 40, 43, 129, 432
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 3 cut(s) 68, 70, 437
AspS9I GGNCC 2 cut(s) 91, 106
AsuHPI GGTGA 1 cut(s) 362
AvaI CYCGRG 2 cut(s) 95, 481
AvaII GGWCC 1 cut(s) 106
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 486
BanII GRGCYC 1 cut(s) 300
BbvI GCAGC 4 cut(s) 27, 30, 141, 419
BccI CCATC 3 cut(s) 341, 503, 580
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 548
BfuAI ACCTGC 1 cut(s) 171
BisI GCNGC 6 cut(s) 41, 44, 115, 130, 356, 433
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 6 cut(s) 42, 45, 116, 131, 357, 434
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 2 cut(s) 95, 481
BmgT120I GGNCC 2 cut(s) 91, 106
BmiI GGNNCC 2 cut(s) 216, 488
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 472
Bpu1102I GCTNAGC 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 4 cut(s) 88, 94, 171, 315
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 235
Bse8I GATNNNNATC 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 4 cut(s) 88, 94, 171, 315
BseGI GGATG 5 cut(s) 244, 352, 406, 463, 572
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 88, 235
BseMII CTCAG 2 cut(s) 182, 429
BsePI GCGCGC 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 4 cut(s) 27, 30, 141, 419
BseYI CCCAGC 1 cut(s) 234
BsgI GTGCAG 3 cut(s) 148, 171, 311
Bsh1236I CGCG 3 cut(s) 16, 68, 70
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 3 cut(s) 93, 154, 314
BshNI GGYRCC 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 155
BsiHKCI CYCGRG 2 cut(s) 95, 481
BsiSI CCGG 2 cut(s) 109, 155
BslI CCNNNNNNNGG 2 cut(s) 88, 235
BsmAI GTCTC 1 cut(s) 548
BsnI GGCC 3 cut(s) 93, 154, 314
BsoBI CYCGRG 2 cut(s) 95, 481
Bsp1286I GDGCHC 1 cut(s) 300
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 1 cut(s) 457
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 4 cut(s) 104, 114, 132, 355
BspANI GGCC 3 cut(s) 93, 154, 314
BspCNI CTCAG 2 cut(s) 183, 428
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 3 cut(s) 16, 68, 70
BspLI GGNNCC 2 cut(s) 216, 488
BspMI ACCTGC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 486
BsrBI CCGCTC 2 cut(s) 114, 355
BssECI CCNNGG 4 cut(s) 88, 94, 171, 315
BssHII GCGCGC 1 cut(s) 66
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 52
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 4 cut(s) 68, 102, 139, 312
BstDEI CTNAG 3 cut(s) 191, 203, 415
BstF5I GGATG 5 cut(s) 244, 352, 406, 463, 572
BstFNI CGCG 3 cut(s) 16, 68, 70
BstHHI GCGC 3 cut(s) 68, 70, 437
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 1 cut(s) 457
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 138, 486
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstUI CGCG 3 cut(s) 16, 68, 70
BstV1I GCAGC 4 cut(s) 27, 30, 141, 419
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 3 cut(s) 93, 154, 314
BtsCI GGATG 5 cut(s) 244, 352, 406, 463, 572
BtsIMutI CAGTG 1 cut(s) 188
BveI ACCTGC 1 cut(s) 171
Cac8I GCNNGC 4 cut(s) 68, 102, 139, 312
CfoI GCGC 3 cut(s) 68, 70, 437
Cfr13I GGNCC 2 cut(s) 91, 106
CpoI CGGWCCG 1 cut(s) 106
CseI GACGC 2 cut(s) 22, 93
Csp6I GTAC 3 cut(s) 48, 226, 568
CspI CGGWCCG 1 cut(s) 106
CviAII CATG 2 cut(s) 451, 578
CviQI GTAC 3 cut(s) 48, 226, 568
DdeI CTNAG 3 cut(s) 191, 203, 415
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DraI TTTAAA 3 cut(s) 285, 475, 607
EaeI YGGCCR 2 cut(s) 152, 312
EagI CGGCCG 1 cut(s) 152
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 152
Eco88I CYCGRG 2 cut(s) 95, 481
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 454, 581
FaiI YATR 8 cut(s) 342, 344, 452, 500, 517, 553, 555, 579
FatI CATG 2 cut(s) 450, 577
FauI CCCGC 1 cut(s) 125
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 6 cut(s) 41, 44, 115, 130, 356, 433
FokI GGATG 5 cut(s) 251, 359, 393, 450, 559
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 41, 44, 115, 130, 356, 433
FspI TGCGCA 1 cut(s) 436
GlaI GCGC 3 cut(s) 67, 69, 436
GluI GCNGC 6 cut(s) 41, 44, 115, 130, 356, 433
GsaI CCCAGC 1 cut(s) 238
HaeIII GGCC 3 cut(s) 93, 154, 314
HapII CCGG 2 cut(s) 109, 155
HgaI GACGC 2 cut(s) 22, 93
HhaI GCGC 3 cut(s) 68, 70, 437
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 2 cut(s) 454, 581
Hin6I GCGC 3 cut(s) 66, 68, 435
HinP1I GCGC 3 cut(s) 66, 68, 435
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 8 cut(s) 25, 72, 143, 148, 160, 322, 443, 588
HpaII CCGG 2 cut(s) 109, 155
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 76, 147
Hpy188I TCNGA 5 cut(s) 30, 334, 418, 548, 560
Hpy188III TCNNGA 2 cut(s) 109, 596
Hpy8I GTNNAC 2 cut(s) 76, 147
Hpy99I CGWCG 5 cut(s) 10, 13, 16, 87, 305
HpyAV CCTTC 2 cut(s) 205, 256
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4IV ACGT 1 cut(s) 610
HpyCH4V TGCA 5 cut(s) 129, 188, 255, 328, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 486
HpyF3I CTNAG 3 cut(s) 191, 203, 415
HpySE526I ACGT 1 cut(s) 610
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 2 cut(s) 454, 581
HspAI GCGC 3 cut(s) 66, 68, 435
Kpn2I TCCGGA 1 cut(s) 108
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 2 cut(s) 111, 214
Lsp1109I GCAGC 4 cut(s) 27, 30, 141, 419
LweI GCATC 1 cut(s) 472
MaeII ACGT 1 cut(s) 610
MalI GATC 1 cut(s) 459
MbiI CCGCTC 2 cut(s) 114, 355
MboI GATC 1 cut(s) 457
MboII GAAGA 1 cut(s) 380
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 300
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 3 cut(s) 426, 438, 470
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 4 cut(s) 81, 142, 152, 169
MnlI CCTC 8 cut(s) 71, 104, 181, 188, 240, 358, 412, 477
Mox20I TGGCCA 1 cut(s) 314
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 314
MseI TTAA 3 cut(s) 284, 474, 606
Msp20I TGGCCA 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 2 cut(s) 109, 155
MspR9I CCNGG 1 cut(s) 89
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 3 cut(s) 16, 68, 70
MwoI GCNNNNNNNGC 2 cut(s) 138, 486
NdeII GATC 1 cut(s) 457
NlaIII CATG 2 cut(s) 454, 581
NlaIV GGNNCC 2 cut(s) 216, 488
NmeAIII GCCGAG 1 cut(s) 130
NsbI TGCGCA 1 cut(s) 436
PaeR7I CTCGAG 1 cut(s) 481
PauI GCGCGC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 300
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 4 cut(s) 25, 322, 443, 588
PkrI GCNGC 6 cut(s) 42, 45, 116, 131, 357, 434
PleI GAGTC 4 cut(s) 80, 142, 151, 168
PpsI GAGTC 4 cut(s) 80, 142, 151, 168
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 234
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 216, 488
PspPI GGNCC 2 cut(s) 91, 106
PspXI VCTCGAGB 1 cut(s) 481
PteI GCGCGC 1 cut(s) 66
RsaI GTAC 3 cut(s) 49, 227, 569
RsaNI GTAC 3 cut(s) 48, 226, 568
Rsr2I CGGWCCG 1 cut(s) 106
RsrII CGGWCCG 1 cut(s) 106
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 3 cut(s) 284, 474, 606
SatI GCNGC 6 cut(s) 41, 44, 115, 130, 356, 433
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 2 cut(s) 91, 106
SchI GAGTC 4 cut(s) 81, 142, 152, 169
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 300
SetI ASST 5 cut(s) 185, 341, 381, 488, 613
SfaNI GCATC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 481
SinI GGWCC 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 3 cut(s) 426, 438, 470
SsiI CCGC 4 cut(s) 104, 114, 132, 355
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 52
TaiI ACGT 1 cut(s) 613
TaqI TCGA 3 cut(s) 146, 294, 482
TasI AATT 3 cut(s) 426, 438, 470
TauI GCSGC 2 cut(s) 117, 358
TfiI GAWTC 4 cut(s) 25, 322, 443, 588
Tru1I TTAA 3 cut(s) 284, 474, 606
Tru9I TTAA 3 cut(s) 284, 474, 606
TscAI CASTG 1 cut(s) 195
TseI GCWGC 4 cut(s) 40, 43, 129, 432
TspDTI ATGAA 2 cut(s) 387, 580
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 195
VpaK11BI GGWCC 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.