FvH4_2g33750

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012457
Physical Location & Seq
Forward (+)
25212282 .. 25213989
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33750.t1

Sequence Viewer

Length: 609 bp
ATGGGATCTCTGAATCCTACGCTTCTCAGAGGCATATCTCTCCTCCGCCTCCGCCTCCACCACGAGCGACTCAGCTTCGGTTTCGCCGCGGCAGCGCCCAGTCGCAGGGCGTGCTGCACCGAGTCGATTCGCGTCTCCGCCGCCGCGGAGGAGAGTGGCTCATCCTCTGCGGAGAATTCCACAGTGCTGAGGGTGAAGGAGGCTCCGAAGTATCCGCGGTGGGAGGACCCAGACTACTGCAAGTGGAAAGATAAGGAAGGCGAGATTCTGAGGGACGTCGAGCCTATTGTTATGCTTGCCAAAGAGATTCTCCATTCCGATAGGTATATGGATGGTGAGCGGCTGTCAGCTGAAGATGAAAAAATTGTGGAGGAGAGGCTTTTGGCTTATCATCCACATTCCGAGGATAAAATTGGCTGCGGAATTGATTCTATAATGGTTGATCGGCATCCCCAATTTAAACAGTCAAGGTGCCTATTTGTCATTAGAACTAATGGTGCGTGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCAACCCATGCAGAAAGGTTCATTCGAGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

203

Amino Acids

23.27

Weight (kDa)

8.2

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 101 - 177 4e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 471
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 4 cut(s) 89, 132, 146, 217
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 105
AluBI AGCT 2 cut(s) 75, 350
AluI AGCT 2 cut(s) 75, 350
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 13
ApeKI GCWGC 3 cut(s) 92, 114, 417
ApoI RAATTY 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 427
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 2 cut(s) 205, 347
AvaII GGWCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 471
BauI CACGAG 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 521
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 101, 104, 404
BccI CCATC 1 cut(s) 326
BciVI GTATCC 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 139
BfoI RGCGCY 1 cut(s) 98
BfuI GTATCC 1 cut(s) 222
BisI GCNGC 8 cut(s) 87, 90, 93, 115, 141, 144, 341, 418
BlsI GCNGC 8 cut(s) 88, 91, 94, 116, 142, 145, 342, 419
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 3 cut(s) 204, 228, 473
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 457
BmuI ACTGGG 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 521
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu10I CCTNAGC 1 cut(s) 188
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 87, 144, 215, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 87, 144, 215, 402
BseGI GGATG 4 cut(s) 161, 337, 391, 448
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 4 cut(s) 40, 85, 179, 260
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 32, 164, 386
BseXI GCAGC 3 cut(s) 101, 104, 404
BsgI GTGCAG 1 cut(s) 100
Bsh1236I CGCG 4 cut(s) 89, 132, 146, 217
BshNI GGYRCC 1 cut(s) 471
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 139
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 287
Bsp143I GATC 2 cut(s) 5, 442
BspCNI CTCAG 4 cut(s) 39, 84, 180, 261
BspFNI CGCG 4 cut(s) 89, 132, 146, 217
BspLI GGNNCC 3 cut(s) 204, 228, 473
BspPI GGATC 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 471
BsrBI CCGCTC 1 cut(s) 340
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 4 cut(s) 87, 144, 215, 402
BssMI GATC 2 cut(s) 5, 442
BssNI GRCGYC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 62
Bst2BI CACGAG 1 cut(s) 62
Bst4CI ACNGT 2 cut(s) 184, 465
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 112, 297
BstDEI CTNAG 4 cut(s) 26, 71, 188, 269
BstDSI CCRYGG 3 cut(s) 87, 144, 215
BstF5I GGATG 4 cut(s) 161, 337, 391, 448
BstFNI CGCG 4 cut(s) 89, 132, 146, 217
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 2 cut(s) 8, 445
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 2 cut(s) 5, 442
BstMWI GCNNNNNNNGC 2 cut(s) 92, 111
BstUI CGCG 4 cut(s) 89, 132, 146, 217
BstV1I GCAGC 3 cut(s) 101, 104, 404
BstV2I GAAGAC 1 cut(s) 521
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuI GTATCC 1 cut(s) 222
BtgI CCRYGG 3 cut(s) 87, 144, 215
BtsCI GGATG 4 cut(s) 161, 337, 391, 448
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 112, 297
CfoI GCGC 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 226
Cfr42I CCGCGG 3 cut(s) 90, 147, 218
CseI GACGC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 553
CviAII CATG 1 cut(s) 563
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 4 cut(s) 26, 71, 188, 269
DpnI GATC 2 cut(s) 7, 444
DpnII GATC 2 cut(s) 5, 442
DraI TTTAAA 2 cut(s) 460, 592
EciI GGCGGA 3 cut(s) 35, 41, 127
Eco47I GGWCC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 1 cut(s) 566
FaiI YATR 8 cut(s) 35, 293, 327, 329, 434, 538, 540, 564
FaqI GGGAC 1 cut(s) 287
FatI CATG 1 cut(s) 562
Fnu4HI GCNGC 8 cut(s) 87, 90, 93, 115, 141, 144, 341, 418
FokI GGATG 4 cut(s) 148, 344, 378, 435
Fsp4HI GCNGC 8 cut(s) 87, 90, 93, 115, 141, 144, 341, 418
GlaI GCGC 1 cut(s) 96
GluI GCNGC 8 cut(s) 87, 90, 93, 115, 141, 144, 341, 418
HaeII RGCGCY 1 cut(s) 98
HgaI GACGC 1 cut(s) 121
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 1 cut(s) 566
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HinfI GANTC 7 cut(s) 13, 69, 122, 127, 265, 307, 428
HphI GGTGA 2 cut(s) 205, 347
Hpy188I TCNGA 7 cut(s) 12, 29, 207, 270, 319, 403, 545
Hpy188III TCNNGA 1 cut(s) 581
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 2 cut(s) 190, 251
HpyCH4III ACNGT 2 cut(s) 184, 465
HpyCH4IV ACGT 2 cut(s) 276, 595
HpyCH4V TGCA 3 cut(s) 117, 240, 566
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 111
HpyF3I CTNAG 4 cut(s) 26, 71, 188, 269
HpySE526I ACGT 2 cut(s) 276, 595
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 1 cut(s) 566
HspAI GCGC 1 cut(s) 95
KspI CCGCGG 3 cut(s) 90, 147, 218
Kzo9I GATC 2 cut(s) 5, 442
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 4 cut(s) 91, 112, 243, 533
Lsp1109I GCAGC 3 cut(s) 101, 104, 404
LweI GCATC 1 cut(s) 457
MaeII ACGT 2 cut(s) 276, 595
MalI GATC 2 cut(s) 7, 444
MbiI CCGCTC 1 cut(s) 340
MboI GATC 2 cut(s) 5, 442
MboII GAAGA 2 cut(s) 365, 521
MflI RGATCY 1 cut(s) 5
MluCI AATT 5 cut(s) 175, 363, 411, 423, 455
MlyI GAGTC 2 cut(s) 63, 131
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 2 cut(s) 459, 591
MspA1I CMGCKG 4 cut(s) 89, 146, 217, 350
MvnI CGCG 4 cut(s) 89, 132, 146, 217
MwoI GCNNNNNNNGC 2 cut(s) 92, 111
NdeII GATC 2 cut(s) 5, 442
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 3 cut(s) 204, 228, 473
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 5 cut(s) 13, 127, 265, 307, 428
PkrI GCNGC 8 cut(s) 88, 91, 94, 116, 142, 145, 342, 419
PleI GAGTC 2 cut(s) 63, 130
PpsI GAGTC 2 cut(s) 63, 130
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
PspN4I GGNNCC 3 cut(s) 204, 228, 473
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PsuI RGATCY 1 cut(s) 5
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SacII CCGCGG 3 cut(s) 90, 147, 218
SaqAI TTAA 2 cut(s) 459, 591
SatI GCNGC 8 cut(s) 87, 90, 93, 115, 141, 144, 341, 418
Sau3AI GATC 2 cut(s) 5, 442
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 2 cut(s) 63, 131
SetI ASST 7 cut(s) 77, 279, 326, 352, 473, 575, 598
SfaNI GCATC 1 cut(s) 457
Sfr303I CCGCGG 3 cut(s) 90, 147, 218
SgrBI CCGCGG 3 cut(s) 90, 147, 218
SinI GGWCC 1 cut(s) 226
Sse9I AATT 5 cut(s) 175, 363, 411, 423, 455
TaaI ACNGT 2 cut(s) 184, 465
TaiI ACGT 2 cut(s) 279, 598
TaqI TCGA 4 cut(s) 125, 279, 532, 580
TasI AATT 5 cut(s) 175, 363, 411, 423, 455
TauI GCSGC 5 cut(s) 89, 92, 143, 146, 343
TfiI GAWTC 5 cut(s) 13, 127, 265, 307, 428
Tru1I TTAA 2 cut(s) 459, 591
Tru9I TTAA 2 cut(s) 459, 591
TscAI CASTG 1 cut(s) 189
TseI GCWGC 3 cut(s) 92, 114, 417
TspDTI ATGAA 2 cut(s) 372, 565
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 427
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.