pycom08g07600

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012457
Physical Location & Seq
Reverse (-)
6132404 .. 6135130
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g07600.1

Sequence Viewer

Length: 633 bp
ATGACGACGACGACGACGACGACGCGAATGGCGACTCTGAATCCGGCGCTGCTGCGAACATTTTCGTTCCTCTGCGCGCGAGTCCACCACCGACGCCTGGGCCTCGGGCTTGCGGTCCCGAGCGGCAGGGCGTGGTGCAGCGTGGCGAGCGAGTCGACTCGGCCGGATAGTCTATCGTCCTCGGCGGACAGTTCCGCGCTGAGAGGAGTGCTGAGCGTGAAGGAGCCGCCGAAGTACCCTCGCTGGGACGACCCTGACTACTGCAAGTGGAAGGGTAAGGAACGGGAGATTTTAAACGACATCGGGCCGGTCGTTTTGCTGGCCAAGGAGATTCTGCACTCTGATAGGTACATGGATGGTGAGCGGCTGACGGAGGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCACACTCAGAGGATAAAATTGGCTGCGCAATTGATTCTATCATGGTTGATCGGCATCCCCAATTTAAACACTCAAGGTGCCTATTTGTCATAAGAACTGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCATCCCATGCAGATATATTCATTCGGGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

211

Amino Acids

24.0

Weight (kDa)

8.73

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 109 - 185 3.5e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 495
AccBSI CCGCTC 2 cut(s) 123, 364
AccI GTMKAC 1 cut(s) 155
AccII CGCG 4 cut(s) 25, 77, 79, 197
AciI CCGC 6 cut(s) 113, 123, 185, 195, 227, 364
AcoI YGGCCR 2 cut(s) 161, 321
AcyI GRCGYC 1 cut(s) 94
AfaI GTAC 3 cut(s) 236, 350, 578
AfiI CCNNNNNNNGG 2 cut(s) 97, 244
AjnI CCWGG 1 cut(s) 96
Ama87I CYCGRG 2 cut(s) 104, 118
AoxI GGCC 4 cut(s) 100, 161, 305, 321
ApeKI GCWGC 4 cut(s) 49, 52, 138, 441
Asp700I GAANNNNTTC 2 cut(s) 61, 402
AspLEI GCGC 5 cut(s) 49, 77, 79, 199, 446
AspS9I GGNCC 3 cut(s) 100, 115, 305
AsuHPI GGTGA 1 cut(s) 371
AvaI CYCGRG 2 cut(s) 104, 118
AvaII GGWCC 1 cut(s) 115
BalI TGGCCA 1 cut(s) 323
BanI GGYRCC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 545
BbvI GCAGC 4 cut(s) 36, 39, 150, 428
BccI CCATC 3 cut(s) 350, 512, 589
BciT130I CCWGG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 50
BisI GCNGC 7 cut(s) 50, 53, 124, 139, 227, 365, 442
BlpI GCTNAGC 1 cut(s) 212
BlsI GCNGC 7 cut(s) 51, 54, 125, 140, 228, 366, 443
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 115
BmeT110I CYCGRG 2 cut(s) 104, 118
BmgT120I GGNCC 3 cut(s) 100, 115, 305
BmiI GGNNCC 3 cut(s) 117, 225, 497
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 481
BpiI GAAGAC 1 cut(s) 545
Bpu1102I GCTNAGC 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 1 cut(s) 94
BsaJI CCNNGG 4 cut(s) 97, 103, 180, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 244
Bse118I RCCGGY 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 4 cut(s) 97, 103, 180, 324
BseGI GGATG 4 cut(s) 361, 415, 472, 581
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 97, 244
BseMII CTCAG 3 cut(s) 191, 203, 438
BsePI GCGCGC 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 219
BseX3I CGGCCG 1 cut(s) 161
BseXI GCAGC 4 cut(s) 36, 39, 150, 428
BseYI CCCAGC 1 cut(s) 243
BsgI GTGCAG 2 cut(s) 157, 320
Bsh1236I CGCG 4 cut(s) 25, 77, 79, 197
Bsh1285I CGRYCG 2 cut(s) 164, 312
BshFI GGCC 4 cut(s) 102, 163, 307, 323
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 2 cut(s) 164, 312
BsiHKCI CYCGRG 2 cut(s) 104, 118
BsiSI CCGG 3 cut(s) 44, 164, 308
BslFI GGGAC 2 cut(s) 101, 260
BslI CCNNNNNNNGG 2 cut(s) 97, 244
BsmFI GGGAC 2 cut(s) 101, 260
BsnI GGCC 4 cut(s) 102, 163, 307, 323
BsoBI CYCGRG 2 cut(s) 104, 118
Bsp143I GATC 1 cut(s) 466
Bsp1720I GCTNAGC 1 cut(s) 212
BspACI CCGC 6 cut(s) 113, 123, 185, 195, 227, 364
BspANI GGCC 4 cut(s) 102, 163, 307, 323
BspCNI CTCAG 3 cut(s) 192, 204, 437
BspFNI CGCG 4 cut(s) 25, 77, 79, 197
BspLI GGNNCC 3 cut(s) 117, 225, 497
BspT107I GGYRCC 1 cut(s) 495
BsrBI CCGCTC 2 cut(s) 123, 364
BsrFI RCCGGY 1 cut(s) 307
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 4 cut(s) 97, 103, 180, 324
BssHII GCGCGC 1 cut(s) 75
BssMI GATC 1 cut(s) 466
BssNI GRCGYC 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 94
BstC8I GCNNGC 4 cut(s) 77, 111, 148, 321
BstDEI CTNAG 3 cut(s) 200, 212, 424
BstF5I GGATG 4 cut(s) 361, 415, 472, 581
BstFNI CGCG 4 cut(s) 25, 77, 79, 197
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 5 cut(s) 49, 77, 79, 199, 446
BstKTI GATC 1 cut(s) 469
BstMBI GATC 1 cut(s) 466
BstMCI CGRYCG 2 cut(s) 164, 312
BstMWI GCNNNNNNNGC 1 cut(s) 147
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 4 cut(s) 25, 77, 79, 197
BstV1I GCAGC 4 cut(s) 36, 39, 150, 428
BstV2I GAAGAC 1 cut(s) 545
BstZI CGGCCG 1 cut(s) 161
BsuRI GGCC 4 cut(s) 102, 163, 307, 323
BtsCI GGATG 4 cut(s) 361, 415, 472, 581
Cac8I GCNNGC 4 cut(s) 77, 111, 148, 321
CfoI GCGC 5 cut(s) 49, 77, 79, 199, 446
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 3 cut(s) 100, 115, 305
CseI GACGC 2 cut(s) 31, 102
Csp6I GTAC 3 cut(s) 235, 349, 577
CviAII CATG 3 cut(s) 352, 460, 587
CviQI GTAC 3 cut(s) 235, 349, 577
DdeI CTNAG 3 cut(s) 200, 212, 424
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DraI TTTAAA 3 cut(s) 294, 484, 616
EaeI YGGCCR 2 cut(s) 161, 321
EagI CGGCCG 1 cut(s) 161
EciI GGCGGA 1 cut(s) 200
EclXI CGGCCG 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 115
Eco52I CGGCCG 1 cut(s) 161
Eco88I CYCGRG 2 cut(s) 104, 118
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 355, 463, 590
FaiI YATR 8 cut(s) 353, 461, 509, 526, 562, 564, 588, 596
FaqI GGGAC 2 cut(s) 101, 260
FatI CATG 3 cut(s) 351, 459, 586
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 7 cut(s) 50, 53, 124, 139, 227, 365, 442
FokI GGATG 4 cut(s) 368, 402, 459, 568
Fsp4HI GCNGC 7 cut(s) 50, 53, 124, 139, 227, 365, 442
FspI TGCGCA 1 cut(s) 445
GlaI GCGC 5 cut(s) 48, 76, 78, 198, 445
GluI GCNGC 7 cut(s) 50, 53, 124, 139, 227, 365, 442
GsaI CCCAGC 1 cut(s) 247
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 4 cut(s) 102, 163, 307, 323
HapII CCGG 3 cut(s) 44, 164, 308
HgaI GACGC 2 cut(s) 31, 102
HhaI GCGC 5 cut(s) 49, 77, 79, 199, 446
Hin1I GRCGYC 1 cut(s) 94
Hin1II CATG 3 cut(s) 355, 463, 590
Hin6I GCGC 5 cut(s) 47, 75, 77, 197, 444
HinP1I GCGC 5 cut(s) 47, 75, 77, 197, 444
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 7 cut(s) 34, 40, 81, 152, 157, 331, 452
HpaII CCGG 3 cut(s) 44, 164, 308
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 2 cut(s) 85, 156
Hpy188I TCNGA 4 cut(s) 39, 343, 427, 569
Hpy188III TCNNGA 2 cut(s) 118, 605
Hpy8I GTNNAC 2 cut(s) 85, 156
Hpy99I CGWCG 7 cut(s) 10, 13, 16, 19, 22, 25, 96
HpyAV CCTTC 2 cut(s) 214, 265
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 4 cut(s) 138, 264, 337, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 147
HpyF3I CTNAG 3 cut(s) 200, 212, 424
HpySE526I ACGT 1 cut(s) 619
Hsp92I GRCGYC 1 cut(s) 94
Hsp92II CATG 3 cut(s) 355, 463, 590
HspAI GCGC 5 cut(s) 47, 75, 77, 197, 444
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 4 cut(s) 36, 39, 150, 428
LweI GCATC 1 cut(s) 481
MaeII ACGT 1 cut(s) 619
MalI GATC 1 cut(s) 468
MbiI CCGCTC 2 cut(s) 123, 364
MboI GATC 1 cut(s) 466
MboII GAAGA 2 cut(s) 389, 545
MfeI CAATTG 1 cut(s) 447
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 3 cut(s) 435, 447, 479
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 4 cut(s) 28, 90, 151, 161
MnlI CCTC 7 cut(s) 80, 113, 190, 197, 249, 367, 421
Mox20I TGGCCA 1 cut(s) 323
MroXI GAANNNNTTC 2 cut(s) 61, 402
MscI TGGCCA 1 cut(s) 323
MseI TTAA 3 cut(s) 293, 483, 615
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 3 cut(s) 44, 164, 308
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 4 cut(s) 25, 77, 79, 197
MwoI GCNNNNNNNGC 1 cut(s) 147
NdeII GATC 1 cut(s) 466
NlaIII CATG 3 cut(s) 355, 463, 590
NlaIV GGNNCC 3 cut(s) 117, 225, 497
NmeAIII GCCGAG 2 cut(s) 139, 161
NsbI TGCGCA 1 cut(s) 445
PauI GCGCGC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 5 cut(s) 11, 14, 17, 29, 309
PdmI GAANNNNTTC 2 cut(s) 61, 402
PfeI GAWTC 3 cut(s) 40, 331, 452
PkrI GCNGC 7 cut(s) 51, 54, 125, 140, 228, 366, 443
PleI GAGTC 4 cut(s) 28, 89, 151, 160
PpsI GAGTC 4 cut(s) 28, 89, 151, 160
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 117, 225, 497
PspPI GGNCC 3 cut(s) 100, 115, 305
PteI GCGCGC 1 cut(s) 75
RsaI GTAC 3 cut(s) 236, 350, 578
RsaNI GTAC 3 cut(s) 235, 349, 577
SalI GTCGAC 1 cut(s) 154
SaqAI TTAA 3 cut(s) 293, 483, 615
SatI GCNGC 7 cut(s) 50, 53, 124, 139, 227, 365, 442
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 3 cut(s) 100, 115, 305
SchI GAGTC 4 cut(s) 28, 90, 151, 161
ScrFI CCNGG 1 cut(s) 98
SetI ASST 4 cut(s) 350, 390, 497, 622
SfaNI GCATC 1 cut(s) 481
SinI GGWCC 1 cut(s) 115
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 3 cut(s) 435, 447, 479
SsiI CCGC 6 cut(s) 113, 123, 185, 195, 227, 364
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 191
TaiI ACGT 1 cut(s) 622
TaqI TCGA 2 cut(s) 155, 556
TasI AATT 3 cut(s) 435, 447, 479
TauI GCSGC 3 cut(s) 126, 229, 367
TfiI GAWTC 3 cut(s) 40, 331, 452
Tru1I TTAA 3 cut(s) 293, 483, 615
Tru9I TTAA 3 cut(s) 293, 483, 615
TseI GCWGC 4 cut(s) 49, 52, 138, 441
TspDTI ATGAA 2 cut(s) 396, 589
TspGWI ACGGA 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 155
XmnI GAANNNNTTC 2 cut(s) 61, 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.