Rw6G041810

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012457
Physical Location & Seq
Reverse (-)
65462490 .. 65465835
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041810.1

Sequence Viewer

Length: 522 bp
ATGATCCACGATACTCCTCCGCCAAACAAATTAAGAGTGATTGCGGAAATGGGATCCTTGAATCCTATGCTTCTCAGAGGCGCATCTCTCCTGCGCGTCCGTCTCCGCCACCACCGGCTGGGGTTTGGGTTTGGGGCGGCGCCGAGTCGCAGGGCGTGCTGCACGGAGTCGAATCGGGCGGAGGAGAGTAGTTCGGACAACTCCTCGACGCTAAGAGGCGTGCTGAGCGTGAACGAGGCACCCAAGTATCCGCGGTGGGACGACCCGGACTACTGCAAGTGGAAAGATAAGGAAGGCGAGATTCTGAGGGACGTGGAGCCTGTAGTTTTGCTTGCCAAAGAGATTCTCCATTCGGATAGGTATATGGATGGTGAGCAGCTAACAGTTGAAGATGAAAAAGTTGTGGTGGAAAGGCTTCTGGCGTATCATCCGCATTCCGAAGATAAAATTGGCTGCGGAATTGATTCTATAATGGTTGATCGGCATCCCAATTCAAACACTCAAGGTGCTTATTTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

173

Amino Acids

19.49

Weight (kDa)

5.97

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 113 - 167 9.7e-16 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 139, 238
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 2 cut(s) 96, 253
AciI CCGC 9 cut(s) 20, 44, 106, 137, 179, 251, 253, 431, 456
AclWI GGATC 2 cut(s) 48, 61
AcyI GRCGYC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 3 cut(s) 61, 389, 495
AjiI CACGTC 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 1 cut(s) 379
AluI AGCT 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 2 cut(s) 48, 61
ApeKI GCWGC 3 cut(s) 159, 376, 453
Asp700I GAANNNNTTC 2 cut(s) 414, 463
AspLEI GCGC 3 cut(s) 83, 96, 142
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 230, 263
BamHI GGATCC 1 cut(s) 53
BanI GGYRCC 2 cut(s) 139, 238
BbvI GCAGC 3 cut(s) 146, 388, 440
BccI CCATC 1 cut(s) 362
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 143
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 4 cut(s) 138, 160, 377, 454
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 4 cut(s) 139, 161, 378, 455
Bme1390I CCNGG 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 4 cut(s) 55, 141, 240, 318
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 2 cut(s) 92, 493
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 486
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse118I RCCGGY 1 cut(s) 114
Bse8I GATNNNNATC 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 251
BseGI GGATG 3 cut(s) 373, 427, 484
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 3 cut(s) 88, 215, 296
BseRI GAGGAG 3 cut(s) 6, 193, 197
BseXI GCAGC 3 cut(s) 146, 388, 440
BseYI CCCAGC 1 cut(s) 118
BsgI GTGCAG 1 cut(s) 145
Bsh1236I CGCG 2 cut(s) 96, 253
BshNI GGYRCC 2 cut(s) 139, 238
BsiSI CCGG 2 cut(s) 115, 266
BslFI GGGAC 2 cut(s) 272, 323
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 2 cut(s) 272, 323
BsmI GAATGC 1 cut(s) 433
Bsp143I GATC 3 cut(s) 3, 53, 478
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 9 cut(s) 20, 44, 106, 137, 179, 251, 253, 431, 456
BspCNI CTCAG 3 cut(s) 87, 216, 297
BspFNI CGCG 2 cut(s) 96, 253
BspLI GGNNCC 4 cut(s) 55, 141, 240, 318
BspPI GGATC 2 cut(s) 48, 61
BspT107I GGYRCC 2 cut(s) 139, 238
BsrFI RCCGGY 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 251
BssMI GATC 3 cut(s) 3, 53, 478
BssNI GRCGYC 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 385
BstACI GRCGYC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 157, 221, 333
BstDEI CTNAG 4 cut(s) 74, 212, 224, 305
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 3 cut(s) 373, 427, 484
BstFNI CGCG 2 cut(s) 96, 253
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 3 cut(s) 83, 96, 142
BstKTI GATC 3 cut(s) 6, 56, 481
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 3 cut(s) 3, 53, 478
BstMWI GCNNNNNNNGC 2 cut(s) 156, 225
BstSCI CCNGG 1 cut(s) 264
BstSFI CTRYAG 1 cut(s) 321
BstUI CGCG 2 cut(s) 96, 253
BstV1I GCAGC 3 cut(s) 146, 388, 440
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuI GTATCC 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 251
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 3 cut(s) 373, 427, 484
Cac8I GCNNGC 3 cut(s) 157, 221, 333
CfoI GCGC 3 cut(s) 83, 96, 142
Cfr10I RCCGGY 1 cut(s) 114
Cfr42I CCGCGG 1 cut(s) 254
CseI GACGC 2 cut(s) 85, 217
CviJI RGCY 5 cut(s) 118, 319, 379, 415, 453
CviKI_1 RGCY 5 cut(s) 118, 319, 379, 415, 453
DdeI CTNAG 4 cut(s) 74, 212, 224, 305
DinI GGCGCC 1 cut(s) 141
DpnI GATC 3 cut(s) 5, 55, 480
DpnII GATC 3 cut(s) 3, 53, 478
EciI GGCGGA 3 cut(s) 9, 95, 194
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 107
FaiI YATR 5 cut(s) 68, 363, 365, 470, 520
FaqI GGGAC 2 cut(s) 272, 323
Fnu4HI GCNGC 4 cut(s) 138, 160, 377, 454
FokI GGATG 3 cut(s) 380, 414, 471
Fsp4HI GCNGC 4 cut(s) 138, 160, 377, 454
GlaI GCGC 3 cut(s) 82, 95, 141
GluI GCNGC 4 cut(s) 138, 160, 377, 454
GsaI CCCAGC 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 143
HapII CCGG 2 cut(s) 115, 266
HgaI GACGC 2 cut(s) 85, 217
HhaI GCGC 3 cut(s) 83, 96, 142
Hin1I GRCGYC 1 cut(s) 140
Hin6I GCGC 3 cut(s) 81, 94, 140
HinP1I GCGC 3 cut(s) 81, 94, 140
HinfI GANTC 7 cut(s) 61, 145, 167, 172, 301, 343, 464
HpaII CCGG 2 cut(s) 115, 266
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 5 cut(s) 77, 196, 306, 355, 439
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 162, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 225
HpyF3I CTNAG 4 cut(s) 74, 212, 224, 305
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 140
HspAI GCGC 3 cut(s) 81, 94, 140
KasI GGCGCC 1 cut(s) 139
KspI CCGCGG 1 cut(s) 254
Kzo9I GATC 3 cut(s) 3, 53, 478
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 7 cut(s) 104, 104, 128, 136, 279, 333, 404
Lsp1109I GCAGC 3 cut(s) 146, 388, 440
LweI GCATC 2 cut(s) 92, 493
MaeII ACGT 1 cut(s) 312
MalI GATC 3 cut(s) 5, 55, 480
MboI GATC 3 cut(s) 3, 53, 478
MboII GAAGA 2 cut(s) 401, 452
MflI RGATCY 1 cut(s) 53
MluCI AATT 4 cut(s) 29, 447, 459, 490
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 2 cut(s) 154, 176
MnlI CCTC 7 cut(s) 27, 71, 175, 209, 214, 229, 300
MroXI GAANNNNTTC 2 cut(s) 414, 463
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 2 cut(s) 115, 266
MspR9I CCNGG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 433
MvnI CGCG 2 cut(s) 96, 253
MwoI GCNNNNNNNGC 2 cut(s) 156, 225
NarI GGCGCC 1 cut(s) 140
NciI CCSGG 1 cut(s) 266
NdeII GATC 3 cut(s) 3, 53, 478
NlaIV GGNNCC 4 cut(s) 55, 141, 240, 318
NmeAIII GCCGAG 1 cut(s) 168
PctI GAATGC 1 cut(s) 433
PdmI GAANNNNTTC 2 cut(s) 414, 463
PfeI GAWTC 5 cut(s) 61, 172, 301, 343, 464
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 4 cut(s) 139, 161, 378, 455
PleI GAGTC 2 cut(s) 153, 175
PluTI GGCGCC 1 cut(s) 143
PpsI GAGTC 2 cut(s) 153, 175
PspFI CCCAGC 1 cut(s) 118
PspN4I GGNNCC 4 cut(s) 55, 141, 240, 318
PsuI RGATCY 1 cut(s) 53
SacII CCGCGG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 4 cut(s) 138, 160, 377, 454
Sau3AI GATC 3 cut(s) 3, 53, 478
SchI GAGTC 2 cut(s) 154, 176
ScrFI CCNGG 1 cut(s) 266
SetI ASST 4 cut(s) 315, 362, 381, 508
SfaNI GCATC 2 cut(s) 92, 493
SfcI CTRYAG 1 cut(s) 321
SfoI GGCGCC 1 cut(s) 141
Sfr303I CCGCGG 1 cut(s) 254
SgrBI CCGCGG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 4 cut(s) 29, 447, 459, 490
SsiI CCGC 9 cut(s) 20, 44, 106, 137, 179, 251, 253, 431, 456
SspDI GGCGCC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 170, 206
TasI AATT 4 cut(s) 29, 447, 459, 490
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 5 cut(s) 61, 172, 301, 343, 464
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseI GCWGC 3 cut(s) 159, 376, 453
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 2 cut(s) 89, 179
Van91I CCANNNNNTGG 1 cut(s) 118
XmnI GAANNNNTTC 2 cut(s) 414, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.