MD15G1077200.v1.1

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012457
Physical Location & Seq
Reverse (-)
5279325 .. 5282352
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1077200.v1.1.491

Sequence Viewer

Length: 654 bp
ATGACGACGACGACGCGAATGGCGACTCTGAGTCCGGTGCTGCTGCGCACAATTTCGTTCCTCTGCGCGCGAGTCCACCATCAGCGCCTGGGCCTTCGCCTGGCGGTCCGGAGTGGCAGGGCGTGCTGTAGCGGGGCGAGCGAGTCGACTAGGGCGGAGGAGCCGCGGGAGAGCCTATTGTCGTCCGCGGACGGGTCCGCGCTGAGAGGAGTGCTGAGCGTGAAGGAGCCGCCGAAGTACCCGCGCTGGGACGACCCTGACTACTGCAAGTGGAAAGATAAGGAACGGGAGATTTTAAACGACATCGAGCCCGTCGTTTTGCTCGCCAAGGAGATTCTGCACTCTGATAGGTATATGGATGGTGAGCAGCTGACGGAGGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCACAATCGAAGGATAAAATTGGCTGCGGAATTGATTCTATCATGGTTGATCGGCATCCCCAATTTAAACACTCAAGGTGCCTATTTGTCATAAGAACTGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATGCGCATGAAATGATATTCGAATTATACCTTGGAAGGGGAATGTCTTTTGTTTTCAGACCTTCAGTGACCTACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

218

Amino Acids

24.96

Weight (kDa)

7.64

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 110 - 186 1e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 47, 579
AccB1I GGYRCC 1 cut(s) 498
AccI GTMKAC 1 cut(s) 146
AccII CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
AccIII TCCGGA 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 621
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 4 cut(s) 100, 192, 247, 610
AhdI GACNNNNNGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 87, 99
AjuI GAANNNNNNNTTGG 2 cut(s) 588, 620
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
AlwNI CAGNNNCTG 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 4 cut(s) 40, 43, 367, 444
Asp700I GAANNNNTTC 2 cut(s) 405, 454
AspLEI GCGC 7 cut(s) 48, 68, 70, 87, 202, 246, 580
AspS9I GGNCC 3 cut(s) 91, 106, 195
AsuHPI GGTGA 1 cut(s) 374
AsuII TTCGAA 1 cut(s) 594
AvaII GGWCC 2 cut(s) 106, 195
BanI GGYRCC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 4 cut(s) 27, 30, 379, 431
BccI CCATC 3 cut(s) 87, 353, 515
BciT130I CCWGG 2 cut(s) 89, 101
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 127
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 6 cut(s) 41, 44, 164, 230, 368, 445
BlpI GCTNAGC 1 cut(s) 215
BlsI GCNGC 6 cut(s) 42, 45, 165, 231, 369, 446
Bme1390I CCNGG 2 cut(s) 89, 101
Bme18I GGWCC 2 cut(s) 106, 195
BmeRI GACNNNNNGTC 1 cut(s) 30
BmgT120I GGNCC 3 cut(s) 91, 106, 195
BmiI GGNNCC 4 cut(s) 162, 196, 228, 500
BmrFI CCNGG 2 cut(s) 89, 101
BmsI GCATC 2 cut(s) 484, 565
BpiI GAAGAC 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 215
Bpu14I TTCGAA 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 478
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 5 cut(s) 88, 164, 186, 327, 604
BsaWI WCCGGW 2 cut(s) 34, 108
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 192, 247, 610
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 89, 101
BseDI CCNNGG 5 cut(s) 88, 164, 186, 327, 604
BseGI GGATG 3 cut(s) 364, 418, 475
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 4 cut(s) 100, 192, 247, 610
BseMII CTCAG 3 cut(s) 20, 194, 206
BsePI GCGCGC 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 173, 222
BseXI GCAGC 4 cut(s) 27, 30, 379, 431
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 498
BsiSI CCGG 2 cut(s) 35, 109
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 4 cut(s) 100, 192, 247, 610
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 1 cut(s) 469
Bsp1720I GCTNAGC 1 cut(s) 215
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 3 cut(s) 21, 195, 207
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
BspLI GGNNCC 4 cut(s) 162, 196, 228, 500
BspT104I TTCGAA 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 498
BssECI CCNNGG 5 cut(s) 88, 164, 186, 327, 604
BssHII GCGCGC 1 cut(s) 66
BssMI GATC 1 cut(s) 469
BssT1I CCWWGG 2 cut(s) 327, 604
Bst2UI CCWGG 2 cut(s) 89, 101
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 4 cut(s) 68, 124, 139, 324
BstDEI CTNAG 3 cut(s) 29, 203, 215
BstDSI CCRYGG 2 cut(s) 164, 186
BstF5I GGATG 3 cut(s) 364, 418, 475
BstFNI CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 7 cut(s) 48, 68, 70, 87, 202, 246, 580
BstKTI GATC 1 cut(s) 472
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 2 cut(s) 123, 138
BstNI CCWGG 2 cut(s) 89, 101
BstSCI CCNGG 2 cut(s) 87, 99
BstSFI CTRYAG 1 cut(s) 127
BstUI CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
BstV1I GCAGC 4 cut(s) 27, 30, 379, 431
BstV2I GAAGAC 1 cut(s) 548
BsuRI GGCC 1 cut(s) 93
BtgI CCRYGG 2 cut(s) 164, 186
BtsCI GGATG 3 cut(s) 364, 418, 475
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 4 cut(s) 68, 124, 139, 324
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 7 cut(s) 48, 68, 70, 87, 202, 246, 580
Cfr13I GGNCC 3 cut(s) 91, 106, 195
Cfr42I CCGCGG 2 cut(s) 167, 189
CpoI CGGWCCG 1 cut(s) 106
CseI GACGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 238
CspI CGGWCCG 1 cut(s) 106
CviAII CATG 2 cut(s) 463, 581
CviJI RGCY 8 cut(s) 93, 163, 174, 229, 310, 370, 406, 444
CviKI_1 RGCY 8 cut(s) 93, 163, 174, 229, 310, 370, 406, 444
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 3 cut(s) 29, 203, 215
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
DraI TTTAAA 2 cut(s) 297, 487
DriI GACNNNNNGTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 30
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 327, 604
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 106, 195
Eco57I CTGAAG 1 cut(s) 621
EcoRII CCWGG 2 cut(s) 87, 99
EcoT14I CCWWGG 2 cut(s) 327, 604
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 2 cut(s) 327, 604
FaeI CATG 2 cut(s) 466, 584
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 462, 580
FauI CCCGC 3 cut(s) 125, 159, 249
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 6 cut(s) 41, 44, 164, 230, 368, 445
FokI GGATG 3 cut(s) 371, 405, 462
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 6 cut(s) 41, 44, 164, 230, 368, 445
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 1 cut(s) 150
FspI TGCGCA 2 cut(s) 47, 579
GlaI GCGC 7 cut(s) 47, 67, 69, 86, 201, 245, 579
GluI GCNGC 6 cut(s) 41, 44, 164, 230, 368, 445
GsaI CCCAGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 93
HapII CCGG 2 cut(s) 35, 109
HgaI GACGC 1 cut(s) 22
HhaI GCGC 7 cut(s) 48, 68, 70, 87, 202, 246, 580
Hin1II CATG 2 cut(s) 466, 584
Hin6I GCGC 7 cut(s) 46, 66, 68, 85, 200, 244, 578
HinP1I GCGC 7 cut(s) 46, 66, 68, 85, 200, 244, 578
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 6 cut(s) 25, 31, 72, 143, 334, 455
HpaII CCGG 2 cut(s) 35, 109
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 76, 147
Hpy188I TCNGA 4 cut(s) 30, 346, 572, 632
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 2 cut(s) 76, 147
Hpy99I CGWCG 4 cut(s) 10, 13, 16, 317
HpyAV CCTTC 5 cut(s) 104, 217, 424, 603, 645
HpyCH4V TGCA 2 cut(s) 267, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 138
HpyF3I CTNAG 3 cut(s) 29, 203, 215
Hsp92II CATG 2 cut(s) 466, 584
HspAI GCGC 7 cut(s) 46, 66, 68, 85, 200, 244, 578
Kpn2I TCCGGA 1 cut(s) 108
KspI CCGCGG 2 cut(s) 167, 189
Kzo9I GATC 1 cut(s) 469
LmnI GCTCC 2 cut(s) 160, 226
Lsp1109I GCAGC 4 cut(s) 27, 30, 379, 431
LweI GCATC 2 cut(s) 484, 565
MaeI CTAG 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 640
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 2 cut(s) 392, 548
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 5 cut(s) 51, 438, 450, 482, 596
MlyI GAGTC 4 cut(s) 19, 40, 81, 152
MnlI CCTC 4 cut(s) 71, 151, 200, 370
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 2 cut(s) 405, 454
MseI TTAA 2 cut(s) 296, 486
MspA1I CMGCKG 3 cut(s) 166, 188, 370
MspI CCGG 2 cut(s) 35, 109
MspR9I CCNGG 2 cut(s) 89, 101
MvaI CCWGG 2 cut(s) 89, 101
MvnI CGCG 7 cut(s) 16, 68, 70, 166, 188, 200, 244
MwoI GCNNNNNNNGC 2 cut(s) 123, 138
NdeII GATC 1 cut(s) 469
NlaIII CATG 2 cut(s) 466, 584
NlaIV GGNNCC 4 cut(s) 162, 196, 228, 500
NmuCI GTSAC 1 cut(s) 640
NsbI TGCGCA 2 cut(s) 47, 579
NspV TTCGAA 1 cut(s) 594
PauI GCGCGC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 312
PdmI GAANNNNTTC 2 cut(s) 405, 454
PfeI GAWTC 2 cut(s) 334, 455
PkrI GCNGC 6 cut(s) 42, 45, 165, 231, 369, 446
PleI GAGTC 4 cut(s) 19, 39, 80, 151
PpsI GAGTC 4 cut(s) 19, 39, 80, 151
Psp6I CCWGG 2 cut(s) 87, 99
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 2 cut(s) 87, 99
PspN4I GGNNCC 4 cut(s) 162, 196, 228, 500
PspPI GGNCC 3 cut(s) 91, 106, 195
PstNI CAGNNNCTG 1 cut(s) 88
PteI GCGCGC 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
Rsr2I CGGWCCG 1 cut(s) 106
RsrII CGGWCCG 1 cut(s) 106
SacII CCGCGG 2 cut(s) 167, 189
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 296, 486
SatI GCNGC 6 cut(s) 41, 44, 164, 230, 368, 445
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 3 cut(s) 91, 106, 195
SchI GAGTC 4 cut(s) 19, 40, 81, 152
ScrFI CCNGG 2 cut(s) 89, 101
SduI GDGCHC 1 cut(s) 312
SetI ASST 7 cut(s) 353, 372, 393, 500, 606, 637, 647
SfaNI GCATC 2 cut(s) 484, 565
SfcI CTRYAG 1 cut(s) 127
Sfr303I CCGCGG 2 cut(s) 167, 189
SfuI TTCGAA 1 cut(s) 594
SgrBI CCGCGG 2 cut(s) 167, 189
SinI GGWCC 2 cut(s) 106, 195
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 5 cut(s) 51, 438, 450, 482, 596
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 87, 99
StyI CCWWGG 2 cut(s) 327, 604
TaqI TCGA 5 cut(s) 146, 306, 428, 559, 594
TasI AATT 5 cut(s) 51, 438, 450, 482, 596
TauI GCSGC 2 cut(s) 166, 232
TfiI GAWTC 2 cut(s) 334, 455
Tru1I TTAA 2 cut(s) 296, 486
Tru9I TTAA 2 cut(s) 296, 486
TscAI CASTG 1 cut(s) 645
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 4 cut(s) 40, 43, 367, 444
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 2 cut(s) 399, 597
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 1 cut(s) 645
VpaK11BI GGWCC 2 cut(s) 106, 195
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 2 cut(s) 405, 454
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.