Prupe.1G430600_v2.0.a1

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012457
Physical Location & Seq
Reverse (-)
36903670 .. 36911240
7571 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G430600.1

Sequence Viewer

Length: 627 bp
ATGACGACGAGAATGGGCACTCTCAACCCTACGCTGCTCAGAAGCATTTCGTTCCTCTGCGTGCGGGTCCAGCACCGGCGTCTGGGCCTTGGGCTGGTGGGCCCGAGTGGTAGGGCGTGGTGCAGCGGCGCGAGCGAGTCGACTCGGGCGGCGGGGCCGGAGGGTGTATTATCCTCTGCGGACAAGTCTACGCTGAGAGGAGTGCTGAGCGTCAAGGAGGCGCCAAAGTACCCGAGGTGGGACGACCCTGACTACTGTAAATGGAAGGATAAGGAAGGGGAGATTCTGAACGACATCGAGCCCCTCATTTTGCTCGCCAAGGAGATTCTTCATTCTGACAGGTATATGGATGGTGAGCGCCTTTCAGCTGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCGCATTCTGAGGATAAAATTGGCTGTGGAATTGATTCTATCATGGTTGATCGACATCCCCAATTTAGACACTCAAGGTGCCTATTTGTCATAAGAACTGATGGTATATGGATCGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCATCCCATGCAGAAAGATTCATTCGAGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

209

Amino Acids

23.73

Weight (kDa)

8.73

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 220, 489
AccI GTMKAC 2 cut(s) 140, 188
AccII CGCG 1 cut(s) 131
AciI CCGC 6 cut(s) 64, 126, 149, 152, 179, 413
AclWI GGATC 1 cut(s) 530
AcuI CTGAAG 1 cut(s) 390
AcyI GRCGYC 2 cut(s) 79, 221
AfaI GTAC 2 cut(s) 230, 572
AfiI CCNNNNNNNGG 3 cut(s) 82, 94, 238
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
AlwI GGATC 1 cut(s) 530
Ama87I CYCGRG 3 cut(s) 103, 144, 232
AoxI GGCC 3 cut(s) 85, 100, 155
ApaI GGGCCC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 34, 123
Asp700I GAANNNNTTC 3 cut(s) 46, 396, 445
AspLEI GCGC 3 cut(s) 131, 223, 360
AspS9I GGNCC 5 cut(s) 67, 85, 100, 101, 155
AsuHPI GGTGA 1 cut(s) 365
AvaI CYCGRG 3 cut(s) 103, 144, 232
AvaII GGWCC 1 cut(s) 67
BaeGI GKGCMC 2 cut(s) 20, 104
BanI GGYRCC 2 cut(s) 220, 489
BanII GRGCYC 2 cut(s) 104, 303
BbsI GAAGAC 1 cut(s) 539
BbvI GCAGC 2 cut(s) 21, 135
BccI CCATC 3 cut(s) 344, 506, 583
BfoI RGCGCY 2 cut(s) 224, 361
BisI GCNGC 4 cut(s) 35, 124, 127, 150
BlpI GCTNAGC 1 cut(s) 206
BlsI GCNGC 4 cut(s) 36, 125, 128, 151
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 3 cut(s) 103, 144, 232
BmgT120I GGNCC 5 cut(s) 67, 85, 100, 101, 155
BmiI GGNNCC 5 cut(s) 68, 102, 156, 222, 491
BpiI GAAGAC 1 cut(s) 539
Bpu1102I GCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 469
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 2 cut(s) 79, 221
BsaJI CCNNGG 3 cut(s) 88, 233, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 94, 238
Bse118I RCCGGY 1 cut(s) 75
Bse8I GATNNNNATC 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 88, 233, 318
BseGI GGATG 4 cut(s) 355, 409, 466, 575
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 82, 94, 238
BseMII CTCAG 4 cut(s) 52, 185, 197, 411
BseRI GAGGAG 1 cut(s) 213
BseSI GKGCMC 2 cut(s) 20, 104
BseXI GCAGC 2 cut(s) 21, 135
BsgI GTGCAG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 3 cut(s) 87, 102, 157
BshNI GGYRCC 2 cut(s) 220, 489
BsiHKCI CYCGRG 3 cut(s) 103, 144, 232
BsiSI CCGG 2 cut(s) 76, 158
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 82, 94, 238
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 87, 102, 157
BsoBI CYCGRG 3 cut(s) 103, 144, 232
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 20, 104, 303
Bsp143I GATC 2 cut(s) 460, 522
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 6 cut(s) 64, 126, 149, 152, 179, 413
BspANI GGCC 3 cut(s) 87, 102, 157
BspCNI CTCAG 4 cut(s) 51, 186, 198, 412
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 5 cut(s) 68, 102, 156, 222, 491
BspPI GGATC 1 cut(s) 530
BspT107I GGYRCC 2 cut(s) 220, 489
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 3 cut(s) 88, 233, 318
BssMI GATC 2 cut(s) 460, 522
BssNI GRCGYC 2 cut(s) 79, 221
BssT1I CCWWGG 2 cut(s) 88, 318
Bst4CI ACNGT 1 cut(s) 257
BstACI GRCGYC 2 cut(s) 79, 221
BstC8I GCNNGC 3 cut(s) 62, 133, 315
BstDEI CTNAG 4 cut(s) 38, 194, 206, 420
BstF5I GGATG 4 cut(s) 355, 409, 466, 575
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 2 cut(s) 224, 361
BstHHI GCGC 3 cut(s) 131, 223, 360
BstKTI GATC 2 cut(s) 463, 525
BstMBI GATC 2 cut(s) 460, 522
BstMWI GCNNNNNNNGC 2 cut(s) 70, 132
BstSLI GKGCMC 2 cut(s) 20, 104
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 21, 135
BstV2I GAAGAC 1 cut(s) 539
BsuRI GGCC 3 cut(s) 87, 102, 157
BtsCI GGATG 4 cut(s) 355, 409, 466, 575
Cac8I GCNNGC 3 cut(s) 62, 133, 315
CfoI GCGC 3 cut(s) 131, 223, 360
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 5 cut(s) 67, 85, 100, 101, 155
CseI GACGC 2 cut(s) 68, 199
Csp6I GTAC 2 cut(s) 229, 571
CviAII CATG 2 cut(s) 454, 581
CviJI RGCY 9 cut(s) 87, 94, 102, 157, 301, 368, 397, 435, 624
CviKI_1 RGCY 9 cut(s) 87, 94, 102, 157, 301, 368, 397, 435, 624
CviQI GTAC 2 cut(s) 229, 571
DdeI CTNAG 4 cut(s) 38, 194, 206, 420
DinI GGCGCC 1 cut(s) 222
DpnI GATC 2 cut(s) 462, 524
DpnII GATC 2 cut(s) 460, 522
DraI TTTAAA 1 cut(s) 610
Eco130I CCWWGG 2 cut(s) 88, 318
Eco24I GRGCYC 2 cut(s) 104, 303
Eco47I GGWCC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 3 cut(s) 103, 144, 232
EcoT14I CCWWGG 2 cut(s) 88, 318
EcoT38I GRGCYC 2 cut(s) 104, 303
EgeI GGCGCC 1 cut(s) 222
EheI GGCGCC 1 cut(s) 222
ErhI CCWWGG 2 cut(s) 88, 318
FaeI CATG 2 cut(s) 457, 584
FaiI YATR 9 cut(s) 345, 347, 455, 503, 518, 520, 556, 558, 582
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 453, 580
FauI CCCGC 2 cut(s) 57, 145
FblI GTMKAC 2 cut(s) 140, 188
Fnu4HI GCNGC 4 cut(s) 35, 124, 127, 150
FokI GGATG 4 cut(s) 362, 396, 453, 562
FriOI GRGCYC 2 cut(s) 104, 303
Fsp4HI GCNGC 4 cut(s) 35, 124, 127, 150
GlaI GCGC 3 cut(s) 130, 222, 359
GluI GCNGC 4 cut(s) 35, 124, 127, 150
HaeII RGCGCY 2 cut(s) 224, 361
HaeIII GGCC 3 cut(s) 87, 102, 157
HapII CCGG 2 cut(s) 76, 158
HgaI GACGC 2 cut(s) 68, 199
HhaI GCGC 3 cut(s) 131, 223, 360
Hin1I GRCGYC 2 cut(s) 79, 221
Hin1II CATG 2 cut(s) 457, 584
Hin6I GCGC 3 cut(s) 129, 221, 358
HinP1I GCGC 3 cut(s) 129, 221, 358
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 6 cut(s) 137, 142, 283, 325, 446, 591
HpaII CCGG 2 cut(s) 76, 158
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 2 cut(s) 141, 189
Hpy188I TCNGA 5 cut(s) 41, 288, 337, 421, 563
Hpy188III TCNNGA 1 cut(s) 599
Hpy8I GTNNAC 2 cut(s) 141, 189
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 259, 269
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 2 cut(s) 123, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 132
HpyF3I CTNAG 4 cut(s) 38, 194, 206, 420
HpySE526I ACGT 1 cut(s) 613
Hsp92I GRCGYC 2 cut(s) 79, 221
Hsp92II CATG 2 cut(s) 457, 584
HspAI GCGC 3 cut(s) 129, 221, 358
KasI GGCGCC 1 cut(s) 220
Kzo9I GATC 2 cut(s) 460, 522
LpnPI CCDG 8 cut(s) 68, 80, 83, 89, 171, 261, 325, 551
Lsp1109I GCAGC 2 cut(s) 21, 135
MaeII ACGT 1 cut(s) 613
MalI GATC 2 cut(s) 462, 524
MboI GATC 2 cut(s) 460, 522
MboII GAAGA 3 cut(s) 320, 383, 539
MhlI GDGCHC 3 cut(s) 20, 104, 303
MluCI AATT 3 cut(s) 429, 441, 473
Mly113I GGCGCC 1 cut(s) 221
MlyI GAGTC 2 cut(s) 136, 146
MnlI CCTC 8 cut(s) 65, 154, 184, 191, 211, 228, 314, 415
MroXI GAANNNNTTC 3 cut(s) 46, 396, 445
MseI TTAA 1 cut(s) 609
MspA1I CMGCKG 2 cut(s) 126, 368
MspI CCGG 2 cut(s) 76, 158
Mva1269I GAATGC 1 cut(s) 415
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 2 cut(s) 70, 132
NarI GGCGCC 1 cut(s) 221
NdeII GATC 2 cut(s) 460, 522
NlaIII CATG 2 cut(s) 457, 584
NlaIV GGNNCC 5 cut(s) 68, 102, 156, 222, 491
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 3 cut(s) 46, 396, 445
PfeI GAWTC 4 cut(s) 283, 325, 446, 591
PkrI GCNGC 4 cut(s) 36, 125, 128, 151
PleI GAGTC 2 cut(s) 136, 145
PluTI GGCGCC 1 cut(s) 224
PpsI GAGTC 2 cut(s) 136, 145
PspN4I GGNNCC 5 cut(s) 68, 102, 156, 222, 491
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 5 cut(s) 67, 85, 100, 101, 155
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 2 cut(s) 230, 572
RsaNI GTAC 2 cut(s) 229, 571
SalI GTCGAC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 4 cut(s) 35, 124, 127, 150
Sau3AI GATC 2 cut(s) 460, 522
Sau96I GGNCC 5 cut(s) 67, 85, 100, 101, 155
SchI GAGTC 2 cut(s) 136, 146
SduI GDGCHC 3 cut(s) 20, 104, 303
SetI ASST 6 cut(s) 239, 344, 370, 384, 491, 616
SfoI GGCGCC 1 cut(s) 222
SgrAI CRCCGGYG 1 cut(s) 75
SinI GGWCC 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
Sse9I AATT 3 cut(s) 429, 441, 473
SsiI CCGC 6 cut(s) 64, 126, 149, 152, 179, 413
SspDI GGCGCC 1 cut(s) 220
StyI CCWWGG 2 cut(s) 88, 318
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 1 cut(s) 616
TaqI TCGA 6 cut(s) 140, 297, 463, 525, 550, 598
TasI AATT 3 cut(s) 429, 441, 473
TauI GCSGC 2 cut(s) 129, 152
TfiI GAWTC 4 cut(s) 283, 325, 446, 591
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TseI GCWGC 2 cut(s) 34, 123
TspDTI ATGAA 3 cut(s) 320, 390, 583
VpaK11BI GGWCC 1 cut(s) 67
XmiI GTMKAC 2 cut(s) 140, 188
XmnI GAANNNNTTC 3 cut(s) 46, 396, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.