pycom15g07190

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012457
Physical Location & Seq
Reverse (-)
4412296 .. 4415020
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g07190.3

Sequence Viewer

Length: 636 bp
ATGACGACGACGACGCGAATGGCGACTCTGAATCCGGTGCTGCTGCGCACAATTTCGTTCCTCTGCGCGCGAGTCCACCATCAGCGCCTGGGCCTTCGCCTGGCGGTCCGGAGTGGCAGGGCGTGCCGCAGCGGGGCGAGCGAGTCGACTAGGGCGGAGGAGCCGCGGGAGAGCCTATTGTCGTCCGCGGACGGGTCCGCGCTGAGAGGAGTGCTGAGCGCGAAGGAGCCGCCGAAGTACCCGCGCTGGGACGACCCTGACTACTGCAAGTGGAAAGATAAGGAACGGGAGATTTTAAACGACATCGAGCCCGTCGTTTTGCTCGCCAAGGAGATTCTGCACTCTGATAGGTATATGGATGGTGAGCAGCTGACGGAGGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCACAATCAAAGGATAAAATTGGCTGCGGAATTGATTCTATCATGGTTGATCGGCATCCCCAATTTAAACACTCAAGGTGCCTATTTGTCATAAGAACTGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCATCCCATGCAGATAGATTCATTCGGGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

212

Amino Acids

24.33

Weight (kDa)

8.89

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 498
AccI GTMKAC 1 cut(s) 146
AccII CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
AccIII TCCGGA 1 cut(s) 108
AfaI GTAC 2 cut(s) 239, 581
AfiI CCNNNNNNNGG 4 cut(s) 100, 133, 192, 247
AjnI CCWGG 2 cut(s) 87, 99
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
AlwNI CAGNNNCTG 1 cut(s) 88
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 5 cut(s) 40, 43, 129, 367, 444
Asp700I GAANNNNTTC 2 cut(s) 405, 454
AspLEI GCGC 7 cut(s) 48, 68, 70, 87, 202, 221, 246
AspS9I GGNCC 3 cut(s) 91, 106, 195
AsuHPI GGTGA 1 cut(s) 374
AvaII GGWCC 2 cut(s) 106, 195
BanI GGYRCC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 5 cut(s) 27, 30, 141, 379, 431
BccI CCATC 4 cut(s) 87, 353, 515, 592
BciT130I CCWGG 2 cut(s) 89, 101
BfaI CTAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 88
BisI GCNGC 8 cut(s) 41, 44, 127, 130, 164, 230, 368, 445
BlpI GCTNAGC 1 cut(s) 215
BlsI GCNGC 8 cut(s) 42, 45, 128, 131, 165, 231, 369, 446
Bme1390I CCNGG 2 cut(s) 89, 101
Bme18I GGWCC 2 cut(s) 106, 195
BmgT120I GGNCC 3 cut(s) 91, 106, 195
BmiI GGNNCC 4 cut(s) 162, 196, 228, 500
BmrFI CCNGG 2 cut(s) 89, 101
BmsI GCATC 1 cut(s) 484
BpiI GAAGAC 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 215
BpuEI CTTGAG 1 cut(s) 478
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 4 cut(s) 88, 164, 186, 327
BsaWI WCCGGW 2 cut(s) 34, 108
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 133, 192, 247
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 2 cut(s) 89, 101
BseDI CCNNGG 4 cut(s) 88, 164, 186, 327
BseGI GGATG 4 cut(s) 364, 418, 475, 584
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 4 cut(s) 100, 133, 192, 247
BseMII CTCAG 2 cut(s) 194, 206
BsePI GCGCGC 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 173, 222
BseXI GCAGC 5 cut(s) 27, 30, 141, 379, 431
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 498
BsiSI CCGG 2 cut(s) 35, 109
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 4 cut(s) 100, 133, 192, 247
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 1 cut(s) 469
Bsp1720I GCTNAGC 1 cut(s) 215
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 2 cut(s) 195, 207
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
BspLI GGNNCC 4 cut(s) 162, 196, 228, 500
BspT107I GGYRCC 1 cut(s) 498
BssECI CCNNGG 4 cut(s) 88, 164, 186, 327
BssHII GCGCGC 1 cut(s) 66
BssMI GATC 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 89, 101
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 4 cut(s) 68, 124, 139, 324
BstDEI CTNAG 2 cut(s) 203, 215
BstDSI CCRYGG 2 cut(s) 164, 186
BstF5I GGATG 4 cut(s) 364, 418, 475, 584
BstFNI CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 7 cut(s) 48, 68, 70, 87, 202, 221, 246
BstKTI GATC 1 cut(s) 472
BstMBI GATC 1 cut(s) 469
BstMWI GCNNNNNNNGC 2 cut(s) 123, 138
BstNI CCWGG 2 cut(s) 89, 101
BstSCI CCNGG 2 cut(s) 87, 99
BstUI CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
BstV1I GCAGC 5 cut(s) 27, 30, 141, 379, 431
BstV2I GAAGAC 1 cut(s) 548
BsuRI GGCC 1 cut(s) 93
BtgI CCRYGG 2 cut(s) 164, 186
BtsCI GGATG 4 cut(s) 364, 418, 475, 584
Cac8I GCNNGC 4 cut(s) 68, 124, 139, 324
CaiI CAGNNNCTG 1 cut(s) 88
CfoI GCGC 7 cut(s) 48, 68, 70, 87, 202, 221, 246
Cfr13I GGNCC 3 cut(s) 91, 106, 195
Cfr42I CCGCGG 2 cut(s) 167, 189
CpoI CGGWCCG 1 cut(s) 106
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 238, 580
CspI CGGWCCG 1 cut(s) 106
CviAII CATG 2 cut(s) 463, 590
CviJI RGCY 9 cut(s) 93, 163, 174, 229, 310, 370, 406, 444, 633
CviKI_1 RGCY 9 cut(s) 93, 163, 174, 229, 310, 370, 406, 444, 633
CviQI GTAC 2 cut(s) 238, 580
DdeI CTNAG 2 cut(s) 203, 215
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
DraI TTTAAA 3 cut(s) 297, 487, 619
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 106, 195
EcoRII CCWGG 2 cut(s) 87, 99
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 466, 593
FaiI YATR 8 cut(s) 354, 356, 464, 512, 529, 565, 567, 591
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 462, 589
FauI CCCGC 3 cut(s) 125, 159, 249
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 8 cut(s) 41, 44, 127, 130, 164, 230, 368, 445
FokI GGATG 4 cut(s) 371, 405, 462, 571
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 8 cut(s) 41, 44, 127, 130, 164, 230, 368, 445
FspBI CTAG 1 cut(s) 150
FspI TGCGCA 1 cut(s) 47
GlaI GCGC 7 cut(s) 47, 67, 69, 86, 201, 220, 245
GluI GCNGC 8 cut(s) 41, 44, 127, 130, 164, 230, 368, 445
GsaI CCCAGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 1 cut(s) 93
HapII CCGG 2 cut(s) 35, 109
HgaI GACGC 1 cut(s) 22
HhaI GCGC 7 cut(s) 48, 68, 70, 87, 202, 221, 246
Hin1II CATG 2 cut(s) 466, 593
Hin6I GCGC 7 cut(s) 46, 66, 68, 85, 200, 219, 244
HinP1I GCGC 7 cut(s) 46, 66, 68, 85, 200, 219, 244
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 7 cut(s) 25, 31, 72, 143, 334, 455, 600
HpaII CCGG 2 cut(s) 35, 109
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 76, 147
Hpy188I TCNGA 3 cut(s) 30, 346, 572
Hpy188III TCNNGA 2 cut(s) 109, 608
Hpy8I GTNNAC 2 cut(s) 76, 147
Hpy99I CGWCG 4 cut(s) 10, 13, 16, 317
HpyAV CCTTC 2 cut(s) 104, 217
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 3 cut(s) 267, 340, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 138
HpyF3I CTNAG 2 cut(s) 203, 215
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 2 cut(s) 466, 593
HspAI GCGC 7 cut(s) 46, 66, 68, 85, 200, 219, 244
Kpn2I TCCGGA 1 cut(s) 108
KspI CCGCGG 2 cut(s) 167, 189
Kzo9I GATC 1 cut(s) 469
LmnI GCTCC 2 cut(s) 160, 226
Lsp1109I GCAGC 5 cut(s) 27, 30, 141, 379, 431
LweI GCATC 1 cut(s) 484
MaeI CTAG 1 cut(s) 150
MaeII ACGT 1 cut(s) 622
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 2 cut(s) 392, 548
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 4 cut(s) 51, 438, 450, 482
MlyI GAGTC 3 cut(s) 19, 81, 152
MnlI CCTC 4 cut(s) 71, 151, 200, 370
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 2 cut(s) 405, 454
MseI TTAA 3 cut(s) 296, 486, 618
MspA1I CMGCKG 4 cut(s) 132, 166, 188, 370
MspI CCGG 2 cut(s) 35, 109
MspR9I CCNGG 2 cut(s) 89, 101
MvaI CCWGG 2 cut(s) 89, 101
MvnI CGCG 8 cut(s) 16, 68, 70, 166, 188, 200, 221, 244
MwoI GCNNNNNNNGC 2 cut(s) 123, 138
NdeII GATC 1 cut(s) 469
NlaIII CATG 2 cut(s) 466, 593
NlaIV GGNNCC 4 cut(s) 162, 196, 228, 500
NsbI TGCGCA 1 cut(s) 47
PauI GCGCGC 1 cut(s) 66
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 312
PdmI GAANNNNTTC 2 cut(s) 405, 454
PfeI GAWTC 4 cut(s) 31, 334, 455, 600
PkrI GCNGC 8 cut(s) 42, 45, 128, 131, 165, 231, 369, 446
PleI GAGTC 3 cut(s) 19, 80, 151
PpsI GAGTC 3 cut(s) 19, 80, 151
Psp6I CCWGG 2 cut(s) 87, 99
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 2 cut(s) 87, 99
PspN4I GGNNCC 4 cut(s) 162, 196, 228, 500
PspPI GGNCC 3 cut(s) 91, 106, 195
PstNI CAGNNNCTG 1 cut(s) 88
PteI GCGCGC 1 cut(s) 66
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 2 cut(s) 239, 581
RsaNI GTAC 2 cut(s) 238, 580
Rsr2I CGGWCCG 1 cut(s) 106
RsrII CGGWCCG 1 cut(s) 106
SacII CCGCGG 2 cut(s) 167, 189
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 3 cut(s) 296, 486, 618
SatI GCNGC 8 cut(s) 41, 44, 127, 130, 164, 230, 368, 445
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 3 cut(s) 91, 106, 195
SchI GAGTC 3 cut(s) 19, 81, 152
ScrFI CCNGG 2 cut(s) 89, 101
SduI GDGCHC 1 cut(s) 312
SetI ASST 5 cut(s) 353, 372, 393, 500, 625
SfaNI GCATC 1 cut(s) 484
Sfr303I CCGCGG 2 cut(s) 167, 189
SgrBI CCGCGG 2 cut(s) 167, 189
SinI GGWCC 2 cut(s) 106, 195
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 4 cut(s) 51, 438, 450, 482
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 87, 99
StyI CCWWGG 1 cut(s) 327
TaiI ACGT 1 cut(s) 625
TaqI TCGA 3 cut(s) 146, 306, 559
TasI AATT 4 cut(s) 51, 438, 450, 482
TauI GCSGC 3 cut(s) 129, 166, 232
TfiI GAWTC 4 cut(s) 31, 334, 455, 600
Tru1I TTAA 3 cut(s) 296, 486, 618
Tru9I TTAA 3 cut(s) 296, 486, 618
TseI GCWGC 5 cut(s) 40, 43, 129, 367, 444
TspDTI ATGAA 2 cut(s) 399, 592
TspGWI ACGGA 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 106, 195
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 2 cut(s) 405, 454
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.