MD08G1093500.v1.1

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012457
Physical Location & Seq
Reverse (-)
7767028 .. 7773614
6587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1093500.v1.1.491

Sequence Viewer

Length: 624 bp
ATGACGACGACGACGCGAATAGCGACTCTGAATCCGGCGCTGCTGCGAAAAATTTCGTTCCTCTGTGCGCGAGTCCACCACCGACGCCTGGGCCTCGGGCTTGCGGTCCCGAGCGGAAGGGCGTGGTGCAGCGTGGCGAGCGAGTCGACTCGGCCGGATAGTCCATCGTCCTCGGCGGACAGTTCCACGCTCAGAGGAGTGCTGAGCGTGAAGGAGCCGCCGAAGTACCCTCGCTGGGACGACCCTGACTACTGCAAGTGGAAGGATAAGGAACGGGAGATTTTAAACGACATCGAGCCCGTCGTTTTGCTGGCTAAGGAGATTCTGCACTCTGATAGGTATATGGATGGTGAGCAGCTGACGGAGGAAGATGAAAAGGTTGTGGTGGAAAGGCTTCTTGCTCATCATCCACACTCGGAGGATAAAATTGGCTGCGGAATTGATTCTGTCATGGTTGATCGGCATCCCCAATTTAGACACTCGAGGTGCCTATTTGTCATAAGAACAGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCATCCCATGCAGATAGATTCATTCGGGAACATTTTAAACGTGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

208

Amino Acids

23.83

Weight (kDa)

8.52

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 106 - 182 2.5e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 486
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 146
AccII CGCG 2 cut(s) 16, 70
AciI CCGC 5 cut(s) 104, 114, 176, 218, 435
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 51
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 2 cut(s) 227, 569
AfiI CCNNNNNNNGG 2 cut(s) 88, 235
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 358
AluI AGCT 1 cut(s) 358
Ama87I CYCGRG 3 cut(s) 95, 109, 481
AoxI GGCC 2 cut(s) 91, 152
ApeKI GCWGC 5 cut(s) 40, 43, 129, 355, 432
ApoI RAATTY 1 cut(s) 51
Asp700I GAANNNNTTC 3 cut(s) 52, 393, 442
AspLEI GCGC 2 cut(s) 40, 70
AspS9I GGNCC 2 cut(s) 91, 106
AsuHPI GGTGA 1 cut(s) 362
AvaI CYCGRG 3 cut(s) 95, 109, 481
AvaII GGWCC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 486
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 536
BbvI GCAGC 5 cut(s) 27, 30, 141, 367, 419
BccI CCATC 4 cut(s) 172, 341, 503, 580
BciT130I CCWGG 1 cut(s) 89
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 6 cut(s) 41, 44, 130, 218, 356, 433
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 6 cut(s) 42, 45, 131, 219, 357, 434
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 3 cut(s) 95, 109, 481
BmgT120I GGNCC 2 cut(s) 91, 106
BmiI GGNNCC 3 cut(s) 108, 216, 488
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 472
BpiI GAAGAC 1 cut(s) 536
Bpu10I CCTNAGC 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 88, 94, 171
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 235
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 3 cut(s) 88, 94, 171
BseGI GGATG 4 cut(s) 352, 406, 463, 572
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 88, 235
BseMII CTCAG 2 cut(s) 194, 205
BseRI GAGGAG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 5 cut(s) 27, 30, 141, 367, 419
BseYI CCCAGC 1 cut(s) 234
BsgI GTGCAG 2 cut(s) 148, 311
Bsh1236I CGCG 2 cut(s) 16, 70
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 93, 154
BshNI GGYRCC 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 155
BsiHKCI CYCGRG 3 cut(s) 95, 109, 481
BsiSI CCGG 2 cut(s) 35, 155
BslFI GGGAC 2 cut(s) 92, 251
BslI CCNNNNNNNGG 2 cut(s) 88, 235
BsmFI GGGAC 2 cut(s) 92, 251
BsnI GGCC 2 cut(s) 93, 154
BsoBI CYCGRG 3 cut(s) 95, 109, 481
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 457
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 5 cut(s) 104, 114, 176, 218, 435
BspANI GGCC 2 cut(s) 93, 154
BspCNI CTCAG 2 cut(s) 195, 204
BspFNI CGCG 2 cut(s) 16, 70
BspLI GGNNCC 3 cut(s) 108, 216, 488
BspT107I GGYRCC 1 cut(s) 486
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 3 cut(s) 88, 94, 171
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 102, 139, 312
BstDEI CTNAG 3 cut(s) 191, 203, 315
BstF5I GGATG 4 cut(s) 352, 406, 463, 572
BstFNI CGCG 2 cut(s) 16, 70
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 2 cut(s) 40, 70
BstKTI GATC 1 cut(s) 460
BstMBI GATC 1 cut(s) 457
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstUI CGCG 2 cut(s) 16, 70
BstV1I GCAGC 5 cut(s) 27, 30, 141, 367, 419
BstV2I GAAGAC 1 cut(s) 536
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 2 cut(s) 93, 154
BtsCI GGATG 4 cut(s) 352, 406, 463, 572
Cac8I GCNNGC 3 cut(s) 102, 139, 312
CfoI GCGC 2 cut(s) 40, 70
Cfr13I GGNCC 2 cut(s) 91, 106
CseI GACGC 2 cut(s) 22, 93
Csp6I GTAC 2 cut(s) 226, 568
CviAII CATG 2 cut(s) 451, 578
CviQI GTAC 2 cut(s) 226, 568
DdeI CTNAG 3 cut(s) 191, 203, 315
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DraI TTTAAA 2 cut(s) 285, 607
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
EciI GGCGGA 1 cut(s) 191
EclXI CGGCCG 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 152
Eco88I CYCGRG 3 cut(s) 95, 109, 481
EcoRII CCWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 300
FaeI CATG 2 cut(s) 454, 581
FaiI YATR 8 cut(s) 342, 344, 452, 500, 517, 553, 555, 579
FaqI GGGAC 2 cut(s) 92, 251
FatI CATG 2 cut(s) 450, 577
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 6 cut(s) 41, 44, 130, 218, 356, 433
FokI GGATG 4 cut(s) 359, 393, 450, 559
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 6 cut(s) 41, 44, 130, 218, 356, 433
GlaI GCGC 2 cut(s) 39, 69
GluI GCNGC 6 cut(s) 41, 44, 130, 218, 356, 433
GsaI CCCAGC 1 cut(s) 238
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 2 cut(s) 93, 154
HapII CCGG 2 cut(s) 35, 155
HgaI GACGC 2 cut(s) 22, 93
HhaI GCGC 2 cut(s) 40, 70
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 2 cut(s) 454, 581
Hin6I GCGC 2 cut(s) 38, 68
HinP1I GCGC 2 cut(s) 38, 68
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 8 cut(s) 25, 31, 72, 143, 148, 322, 443, 588
HpaII CCGG 2 cut(s) 35, 155
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 76, 147
Hpy188I TCNGA 5 cut(s) 30, 194, 334, 418, 560
Hpy188III TCNNGA 2 cut(s) 109, 596
Hpy8I GTNNAC 2 cut(s) 76, 147
Hpy99I CGWCG 5 cut(s) 10, 13, 16, 87, 305
HpyAV CCTTC 3 cut(s) 111, 205, 256
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 610
HpyCH4V TGCA 4 cut(s) 129, 255, 328, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 3 cut(s) 191, 203, 315
HpySE526I ACGT 1 cut(s) 610
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 2 cut(s) 454, 581
HspAI GCGC 2 cut(s) 38, 68
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 8 cut(s) 48, 74, 101, 168, 220, 258, 296, 548
Lsp1109I GCAGC 5 cut(s) 27, 30, 141, 367, 419
LweI GCATC 1 cut(s) 472
MaeII ACGT 1 cut(s) 610
MalI GATC 1 cut(s) 459
MbiI CCGCTC 1 cut(s) 114
MboI GATC 1 cut(s) 457
MboII GAAGA 2 cut(s) 380, 536
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 4 cut(s) 51, 426, 438, 470
MlyI GAGTC 4 cut(s) 19, 81, 142, 152
MnlI CCTC 8 cut(s) 71, 104, 181, 188, 240, 358, 412, 477
MroXI GAANNNNTTC 3 cut(s) 52, 393, 442
MseI TTAA 2 cut(s) 284, 606
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 2 cut(s) 35, 155
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 2 cut(s) 16, 70
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 1 cut(s) 457
NlaIII CATG 2 cut(s) 454, 581
NlaIV GGNNCC 3 cut(s) 108, 216, 488
NmeAIII GCCGAG 2 cut(s) 130, 152
PaeR7I CTCGAG 1 cut(s) 481
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 300
PdmI GAANNNNTTC 3 cut(s) 52, 393, 442
PfeI GAWTC 4 cut(s) 31, 322, 443, 588
PkrI GCNGC 6 cut(s) 42, 45, 131, 219, 357, 434
PleI GAGTC 4 cut(s) 19, 80, 142, 151
PpsI GAGTC 4 cut(s) 19, 80, 142, 151
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 234
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 108, 216, 488
PspPI GGNCC 2 cut(s) 91, 106
PspXI VCTCGAGB 1 cut(s) 481
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 2 cut(s) 227, 569
RsaNI GTAC 2 cut(s) 226, 568
SalI GTCGAC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 284, 606
SatI GCNGC 6 cut(s) 41, 44, 130, 218, 356, 433
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 2 cut(s) 91, 106
SchI GAGTC 4 cut(s) 19, 81, 142, 152
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 300
SetI ASST 5 cut(s) 341, 360, 381, 488, 613
SfaNI GCATC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 481
SinI GGWCC 1 cut(s) 106
SlaI CTCGAG 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 4 cut(s) 51, 426, 438, 470
SsiI CCGC 5 cut(s) 104, 114, 176, 218, 435
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 613
TaqI TCGA 4 cut(s) 146, 294, 482, 547
TasI AATT 4 cut(s) 51, 426, 438, 470
TauI GCSGC 1 cut(s) 220
TfiI GAWTC 4 cut(s) 31, 322, 443, 588
Tru1I TTAA 2 cut(s) 284, 606
Tru9I TTAA 2 cut(s) 284, 606
TseI GCWGC 5 cut(s) 40, 43, 129, 355, 432
TspDTI ATGAA 2 cut(s) 387, 580
TspGWI ACGGA 1 cut(s) 377
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 146
XmnI GAANNNNTTC 3 cut(s) 52, 393, 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.