RLG00000010617

Protein DCL, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012457
Physical Location & Seq
Forward (+)
2990134 .. 2995056
4923 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010617

Sequence Viewer

Length: 1050 bp
ATGTCAGCGGCCCTAAAAGATCAAATCGAAATCAACCCCCAATCTCCGCAGTCCTTGGTCGGCCCCCTTGGAGATCCAGCGAAATCTTCCGGCCAACCAACAACGAGCAATGATGGATCGTCCATTAAGCTTCGCCCCAAAATCGAACCCAGCCAAAATGGACGCTCATCGGGGAGTTCGAAGACCAACCCATCCACCGTAACACCATCCTCCCAACGAAGCTCCAGGAAGCTAGACAAATCAGCGCCGTCAGAGGAAGAAAGACAAGCCCCAGCCCAAGGCGGCTCTCCATATGATAAATCGAACACAACAAGTAGAACGAGACAGGTAGCCACAAGGCTAATGACCTGGCCATCAAGAACAAATCAAAATCAAACCGGGAGAAGTGACGCACACCATGATCCACGATACTCCTCCGCCAAACAAAGACTGAGCGACATGGGATCCTTGAATCCTATGCTTCTCAGAGGCGTATCTCTCCTCCGCGTCCGTCTCCACCCCCACCGGCTGGGCTTTGGGTTTGGGATTGCGGCGGCGCCGAGTCGCAGGGCGTGCTGCACCGAGTCGATTCGGGCGGTGGAGAGTAGTTCGTCCTCTGCGGACAACTCCTCGACGCTAAGAGGCGTGCTGAGCGTGAACGAGGCACCCAAGTATCCGCGGTGGGACGACCCTGACTACTGCAAGTGGAAAGACAAGGAAGGCGAGATTCTGAGGGACGTGGAGCCTATAGTTTTGCTTGCCAAGGAGATTCTCCATTCGGATAGGTATATGGATGGTGAGCAGCTAACACTTGAAGATGAAAAAGTTGTGGTGGAAAGGCTTCTGGCGTATCATCCGCATTCCGAAGATAAAATTGGCTGCGGAATTGATTCTATAATGGTTGATCGGCATCCCCAATTCAAACACTCAAGGTGCCTATTTGTCATAAGAACTGATGGTGTATGGATAGACTTCTCCTACCAGAAGTGTCTTCGAGCATATATCCGAGATAAGTACCCAACCCATGCAGAAAGATTCATTCGAGAACATTTTAAACGCGGTAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3223

Protein Analysis

350

Amino Acids

38.97

Weight (kDa)

9.31

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 248 - 324 3.5e-31 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012457)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46630
fragaria_vesca FvH4_2g33750
malus_domestica MD08G1093000.v1.1 MD08G1093500.v1.1 MD15G1077200.v1.1
prunus_persica Prupe.1G430600_v2.0.a1
pyrus_communis pycom08g07580 pycom08g07600 pycom15g07190
rosa_chinensis RchiOBHm_Chr6g0308801
rosa_laevigata RLG00000010617
rosa_multiflora Rmu_sc0004046.1_g000005
rosa_roxburghii Rroxscaffold_7G00159910
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479600 Rh6BG489100 Rh6CG494600 Rh6DG479900
rosa_wichuraiana Rw6G041810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 535, 643, 912
AccB7I CCANNNNNTGG 1 cut(s) 508
AccII CGCG 3 cut(s) 486, 658, 1038
AclWI GGATC 5 cut(s) 68, 124, 395, 438, 451
AcoI YGGCCR 2 cut(s) 91, 350
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 1 cut(s) 995
AfiI CCNNNNNNNGG 2 cut(s) 278, 508
AgsI TTSAA 3 cut(s) 451, 794, 901
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 2 cut(s) 224, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 837, 869
AluBI AGCT 4 cut(s) 130, 222, 232, 784
AluI AGCT 4 cut(s) 130, 222, 232, 784
Alw26I GTCTC 2 cut(s) 316, 497
AlwI GGATC 5 cut(s) 68, 124, 395, 438, 451
AoxI GGCC 4 cut(s) 9, 61, 91, 350
ApeKI GCWGC 3 cut(s) 555, 781, 858
Asp700I GAANNNNTTC 2 cut(s) 819, 868
AspLEI GCGC 2 cut(s) 247, 538
AspS9I GGNCC 2 cut(s) 10, 62
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 788
AsuII TTCGAA 1 cut(s) 179
BaeI ACNNNNGTAYC 2 cut(s) 635, 668
BalI TGGCCA 1 cut(s) 352
BamHI GGATCC 1 cut(s) 443
BanI GGYRCC 3 cut(s) 535, 643, 912
BbsI GAAGAC 2 cut(s) 188, 962
BbvI GCAGC 3 cut(s) 542, 793, 845
BccI CCATC 6 cut(s) 107, 199, 214, 361, 767, 929
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 2 cut(s) 226, 349
BciVI GTATCC 1 cut(s) 663
BcnI CCSGG 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 316, 497
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 726
BfoI RGCGCY 2 cut(s) 248, 539
BfuI GTATCC 1 cut(s) 663
BisI GCNGC 7 cut(s) 9, 283, 531, 534, 556, 782, 859
BlpI GCTNAGC 1 cut(s) 629
BlsI GCNGC 7 cut(s) 10, 284, 532, 535, 557, 783, 860
Bme1390I CCNGG 3 cut(s) 226, 349, 379
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 10, 62
BmiI GGNNCC 6 cut(s) 64, 445, 537, 645, 723, 914
BmrFI CCNGG 3 cut(s) 226, 349, 379
BmsI GCATC 1 cut(s) 898
BpiI GAAGAC 2 cut(s) 188, 962
BpmI CTGGAG 1 cut(s) 208
Bpu1102I GCTNAGC 1 cut(s) 629
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 892
BpuMI CCSGG 1 cut(s) 379
BsaBI GATNNNNATC 1 cut(s) 888
BsaHI GRCGYC 1 cut(s) 536
BsaJI CCNNGG 5 cut(s) 54, 67, 277, 656, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 508
Bse118I RCCGGY 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 115
Bse8I GATNNNNATC 1 cut(s) 888
BseBI CCWGG 2 cut(s) 226, 349
BseDI CCNNGG 5 cut(s) 54, 67, 277, 656, 741
BseGI GGATG 5 cut(s) 191, 206, 778, 832, 889
BseJI GATNNNNATC 1 cut(s) 888
BseLI CCNNNNNNNGG 2 cut(s) 278, 508
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 4 cut(s) 422, 478, 620, 701
BseRI GAGGAG 3 cut(s) 403, 470, 598
BseXI GCAGC 3 cut(s) 542, 793, 845
BseYI CCCAGC 3 cut(s) 149, 271, 508
BsgI GTGCAG 1 cut(s) 541
Bsh1236I CGCG 3 cut(s) 486, 658, 1038
BshFI GGCC 4 cut(s) 11, 63, 93, 352
BshNI GGYRCC 3 cut(s) 535, 643, 912
BsiSI CCGG 3 cut(s) 90, 378, 505
BslFI GGGAC 2 cut(s) 677, 728
BslI CCNNNNNNNGG 2 cut(s) 278, 508
BsmAI GTCTC 2 cut(s) 316, 497
BsmBI CGTCTC 1 cut(s) 497
BsmFI GGGAC 2 cut(s) 677, 728
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 4 cut(s) 11, 63, 93, 352
Bsp119I TTCGAA 1 cut(s) 179
Bsp143I GATC 6 cut(s) 19, 73, 116, 400, 443, 883
Bsp1720I GCTNAGC 1 cut(s) 629
BspANI GGCC 4 cut(s) 11, 63, 93, 352
BspCNI CTCAG 4 cut(s) 423, 477, 621, 702
BspFNI CGCG 3 cut(s) 486, 658, 1038
BspLI GGNNCC 6 cut(s) 64, 445, 537, 645, 723, 914
BspPI GGATC 5 cut(s) 68, 124, 395, 438, 451
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 3 cut(s) 535, 643, 912
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 5 cut(s) 54, 67, 277, 656, 741
BssMI GATC 6 cut(s) 19, 73, 116, 400, 443, 883
BssNI GRCGYC 1 cut(s) 536
BssT1I CCWWGG 4 cut(s) 54, 67, 277, 741
Bst2UI CCWGG 2 cut(s) 226, 349
Bst4CI ACNGT 1 cut(s) 199
BstACI GRCGYC 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 553, 626, 738
BstDEI CTNAG 5 cut(s) 431, 464, 617, 629, 710
BstDSI CCRYGG 1 cut(s) 656
BstF5I GGATG 5 cut(s) 191, 206, 778, 832, 889
BstFNI CGCG 3 cut(s) 486, 658, 1038
BstH2I RGCGCY 2 cut(s) 248, 539
BstHHI GCGC 2 cut(s) 247, 538
BstKTI GATC 6 cut(s) 22, 76, 119, 403, 446, 886
BstMAI GTCTC 2 cut(s) 316, 497
BstMBI GATC 6 cut(s) 19, 73, 116, 400, 443, 883
BstMWI GCNNNNNNNGC 3 cut(s) 552, 630, 1044
BstNI CCWGG 2 cut(s) 226, 349
BstSCI CCNGG 3 cut(s) 224, 347, 377
BstSFI CTRYAG 1 cut(s) 726
BstUI CGCG 3 cut(s) 486, 658, 1038
BstV1I GCAGC 3 cut(s) 542, 793, 845
BstV2I GAAGAC 2 cut(s) 188, 962
BstX2I RGATCY 2 cut(s) 73, 443
BstYI RGATCY 2 cut(s) 73, 443
BsuI GTATCC 1 cut(s) 663
BsuRI GGCC 4 cut(s) 11, 63, 93, 352
BtgI CCRYGG 1 cut(s) 656
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 5 cut(s) 191, 206, 778, 832, 889
Cac8I GCNNGC 3 cut(s) 553, 626, 738
CfoI GCGC 2 cut(s) 247, 538
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 2 cut(s) 10, 62
Cfr42I CCGCGG 1 cut(s) 659
CseI GACGC 4 cut(s) 171, 398, 475, 622
Csp6I GTAC 1 cut(s) 994
CviAII CATG 3 cut(s) 398, 439, 1004
CviQI GTAC 1 cut(s) 994
DdeI CTNAG 5 cut(s) 431, 464, 617, 629, 710
DinI GGCGCC 1 cut(s) 537
DpnI GATC 6 cut(s) 21, 75, 118, 402, 445, 885
DpnII GATC 6 cut(s) 19, 73, 116, 400, 443, 883
DraI TTTAAA 1 cut(s) 1033
EaeI YGGCCR 2 cut(s) 91, 350
EciI GGCGGA 1 cut(s) 406
Eco130I CCWWGG 4 cut(s) 54, 67, 277, 741
EcoRII CCWGG 2 cut(s) 224, 347
EcoT14I CCWWGG 4 cut(s) 54, 67, 277, 741
EgeI GGCGCC 1 cut(s) 537
EheI GGCGCC 1 cut(s) 537
ErhI CCWWGG 4 cut(s) 54, 67, 277, 741
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 3 cut(s) 401, 442, 1007
FaqI GGGAC 2 cut(s) 677, 728
FatI CATG 3 cut(s) 397, 438, 1003
FauNDI CATATG 1 cut(s) 292
Fnu4HI GCNGC 7 cut(s) 9, 283, 531, 534, 556, 782, 859
FokI GGATG 5 cut(s) 178, 193, 785, 819, 876
Fsp4HI GCNGC 7 cut(s) 9, 283, 531, 534, 556, 782, 859
FspBI CTAG 1 cut(s) 233
GlaI GCGC 2 cut(s) 246, 537
GluI GCNGC 7 cut(s) 9, 283, 531, 534, 556, 782, 859
GsaI CCCAGC 3 cut(s) 153, 275, 512
GsuI CTGGAG 1 cut(s) 208
HaeII RGCGCY 2 cut(s) 248, 539
HaeIII GGCC 4 cut(s) 11, 63, 93, 352
HapII CCGG 3 cut(s) 90, 378, 505
HgaI GACGC 4 cut(s) 171, 398, 475, 622
HhaI GCGC 2 cut(s) 247, 538
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 3 cut(s) 401, 442, 1007
Hin6I GCGC 2 cut(s) 245, 536
HinP1I GCGC 2 cut(s) 245, 536
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 8 cut(s) 451, 541, 563, 568, 706, 748, 869, 1014
HpaII CCGG 3 cut(s) 90, 378, 505
HphI GGTGA 1 cut(s) 788
Hpy166II GTNNAC 1 cut(s) 637
Hpy188I TCNGA 6 cut(s) 253, 467, 711, 760, 844, 986
Hpy188III TCNNGA 2 cut(s) 357, 1022
Hpy8I GTNNAC 1 cut(s) 637
Hpy99I CGWCG 1 cut(s) 616
HpyAV CCTTC 1 cut(s) 692
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 3 cut(s) 558, 681, 1007
HpyF10VI GCNNNNNNNGC 3 cut(s) 552, 630, 1044
HpyF3I CTNAG 5 cut(s) 431, 464, 617, 629, 710
HpySE526I ACGT 1 cut(s) 717
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 3 cut(s) 401, 442, 1007
HspAI GCGC 2 cut(s) 245, 536
KasI GGCGCC 1 cut(s) 535
KspI CCGCGG 1 cut(s) 659
Kzo9I GATC 6 cut(s) 19, 73, 116, 400, 443, 883
LmnI GCTCC 2 cut(s) 227, 721
Lsp1109I GCAGC 3 cut(s) 542, 793, 845
LweI GCATC 1 cut(s) 898
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 2 cut(s) 199, 386
MalI GATC 6 cut(s) 21, 75, 118, 402, 445, 885
MboI GATC 6 cut(s) 19, 73, 116, 400, 443, 883
MboII GAAGA 6 cut(s) 78, 193, 269, 806, 857, 962
MflI RGATCY 2 cut(s) 73, 443
MlsI TGGCCA 1 cut(s) 352
MluCI AATT 3 cut(s) 852, 864, 896
MluNI TGGCCA 1 cut(s) 352
Mly113I GGCGCC 1 cut(s) 536
MlyI GAGTC 2 cut(s) 550, 572
Mox20I TGGCCA 1 cut(s) 352
MroXI GAANNNNTTC 2 cut(s) 819, 868
MscI TGGCCA 1 cut(s) 352
MseI TTAA 2 cut(s) 126, 1032
Msp20I TGGCCA 1 cut(s) 352
MspA1I CMGCKG 2 cut(s) 8, 658
MspI CCGG 3 cut(s) 90, 378, 505
MspR9I CCNGG 3 cut(s) 226, 349, 379
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 2 cut(s) 226, 349
MvnI CGCG 3 cut(s) 486, 658, 1038
MwoI GCNNNNNNNGC 3 cut(s) 552, 630, 1044
NarI GGCGCC 1 cut(s) 536
NciI CCSGG 1 cut(s) 379
NdeI CATATG 1 cut(s) 292
NdeII GATC 6 cut(s) 19, 73, 116, 400, 443, 883
NlaIII CATG 3 cut(s) 401, 442, 1007
NlaIV GGNNCC 6 cut(s) 64, 445, 537, 645, 723, 914
NmeAIII GCCGAG 1 cut(s) 564
NmuCI GTSAC 1 cut(s) 386
NspV TTCGAA 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PctI GAATGC 1 cut(s) 838
PdmI GAANNNNTTC 2 cut(s) 819, 868
PfeI GAWTC 6 cut(s) 451, 568, 706, 748, 869, 1014
PflMI CCANNNNNTGG 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 7 cut(s) 10, 284, 532, 535, 557, 783, 860
PleI GAGTC 2 cut(s) 549, 571
PluTI GGCGCC 1 cut(s) 539
PpsI GAGTC 2 cut(s) 549, 571
Psp6I CCWGG 2 cut(s) 224, 347
PspFI CCCAGC 3 cut(s) 149, 271, 508
PspGI CCWGG 2 cut(s) 224, 347
PspN4I GGNNCC 6 cut(s) 64, 445, 537, 645, 723, 914
PspPI GGNCC 2 cut(s) 10, 62
PsuI RGATCY 2 cut(s) 73, 443
RsaI GTAC 1 cut(s) 995
RsaNI GTAC 1 cut(s) 994
SacII CCGCGG 1 cut(s) 659
SaqAI TTAA 2 cut(s) 126, 1032
SatI GCNGC 7 cut(s) 9, 283, 531, 534, 556, 782, 859
Sau3AI GATC 6 cut(s) 19, 73, 116, 400, 443, 883
Sau96I GGNCC 2 cut(s) 10, 62
SchI GAGTC 2 cut(s) 550, 572
ScrFI CCNGG 3 cut(s) 226, 349, 379
SetI ASST 9 cut(s) 132, 224, 234, 330, 350, 720, 767, 786, 914
SfaNI GCATC 1 cut(s) 898
SfcI CTRYAG 1 cut(s) 726
SfoI GGCGCC 1 cut(s) 537
Sfr303I CCGCGG 1 cut(s) 659
SfuI TTCGAA 1 cut(s) 179
SgrBI CCGCGG 1 cut(s) 659
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
Sse9I AATT 3 cut(s) 852, 864, 896
SspDI GGCGCC 1 cut(s) 535
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 224, 347, 377
StyI CCWWGG 4 cut(s) 54, 67, 277, 741
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 720
TaqI TCGA 8 cut(s) 27, 144, 179, 302, 566, 611, 973, 1021
TasI AATT 3 cut(s) 852, 864, 896
TauI GCSGC 4 cut(s) 11, 285, 533, 536
TfiI GAWTC 6 cut(s) 451, 568, 706, 748, 869, 1014
Tru1I TTAA 2 cut(s) 126, 1032
Tru9I TTAA 2 cut(s) 126, 1032
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 3 cut(s) 555, 781, 858
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 2 cut(s) 813, 1006
TspGWI ACGGA 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 508
XmnI GAANNNNTTC 2 cut(s) 819, 868
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.