AT3G48210

Chromosome segregation protein Spc25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007257
Physical Location & Seq
Forward (+)
17849351 .. 17851785
2435 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G48210.1

Sequence Viewer

Length: 948 bp
ATGGAACAAATTAGCAACATCGCTGGTGGAGACACAACGAAAGAGACTATGGCGTCTCTGGGTCTCATCTGTGAGAAAGATATTCATGAACAGCGTCTTAAAATCGATTCTTTCATCGCTTCTCCTTTCCGGAGATCGATGAACTCTTTAGTGGAGCGAGCTCAAGCCACTGCTCAAAGCCAAGTGGAACTAATGAATTTGAAAGCGGATCTGAGAGAAGCAGAGGATGAGCTAGTTAAAGTGTTGGCAGTGAAAACCCGTAAAGAGGCACGGCAAATGGGGATAAGAGATTCCATTTCTGCAACACAATCTAGGATTGAAGTACTTAGAAGAAATTTGCAGCTACAGAAGTCAAAGAAGGATGATTCTGTGAGAATTATCTCCCAACAGTTGCAAGCCTTGTCAAAATCGAAGGACAACGCTGGTAAAGTCACCGAAGACAAAGCAGATATTCATGAAGCAATCTCTTGGTATAATCATGCTCTCGGTTTTCACGTTGAAGCTGGACATGGAGTTAAATTCACATTCACCAACATTGATGCAAAAAGGCCGACCCGCGAATTTTCGTTCACAGTTCACTATGGAAATGACATCTACACACTATTGGATAGCGATCTGCAATTGGATTACATCAACGAGATGGTTCAAGAATTGAATAAAACCAATGACCTTTTCAGATTTGTTCGTCTGATGAGGGAGCAGTTTCTGAAATCCACTTTATCTGAGCTGCCAACACATTCAGGACAACTGCAACAGGAAACGTCTGCCATATCTGCTTCAGCTCCAGCTATATCATTTTCCACTGATACAAACATGTCAACTCCAGAAAACAAAAGATCTAAGGTTCAAGTGAATCGGCGACAAAAAAGAGGTAGCGAATCACCACTCCTAGCTCCTGTATCCACATCTGCCACTCGCCGTTCTTCTCGCTTTAAGGGTAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

315

Amino Acids

35.58

Weight (kDa)

9.45

Isoelectric Point (pI)

57.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 168 - 235 1.3e-20 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 52
AccII CGCG 1 cut(s) 558
AccIII TCCGGA 1 cut(s) 129
AciI CCGC 2 cut(s) 206, 556
AclWI GGATC 1 cut(s) 216
AcsI RAATTY 4 cut(s) 196, 334, 518, 560
AcuI CTGAAG 1 cut(s) 762
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 265
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 6 cut(s) 202, 320, 500, 647, 655, 848
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AluBI AGCT 8 cut(s) 161, 232, 343, 503, 727, 782, 788, 893
AluI AGCT 8 cut(s) 161, 232, 343, 503, 727, 782, 788, 893
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 4 cut(s) 24, 38, 60, 68
AlwI GGATC 1 cut(s) 216
AlwNI CAGNNNCTG 1 cut(s) 706
Aor13HI TCCGGA 1 cut(s) 129
AoxI GGCC 1 cut(s) 548
ApeKI GCWGC 2 cut(s) 340, 727
ApoI RAATTY 4 cut(s) 196, 334, 518, 560
AsuHPI GGTGA 3 cut(s) 424, 520, 873
BanII GRGCYC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 352, 714
BccI CCATC 1 cut(s) 634
BceAI ACGGC 2 cut(s) 287, 903
BciVI GTATCC 1 cut(s) 910
BcoDI GTCTC 4 cut(s) 24, 38, 60, 68
BfaI CTAG 3 cut(s) 233, 312, 890
BfmI CTRYAG 1 cut(s) 344
BfuI GTATCC 1 cut(s) 910
BglII AGATCT 1 cut(s) 836
BisI GCNGC 2 cut(s) 341, 728
BlsI GCNGC 2 cut(s) 342, 729
BmcAI AGTACT 1 cut(s) 324
BmsI GCATC 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 2 cut(s) 768, 807
BpuEI CTTGAG 1 cut(s) 147
Bsa29I ATCGAT 2 cut(s) 105, 137
BsaBI GATNNNNATC 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 612
BseAI TCCGGA 1 cut(s) 129
BseCI ATCGAT 2 cut(s) 105, 137
BseGI GGATG 2 cut(s) 232, 367
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 203, 714
BseXI GCAGC 2 cut(s) 352, 714
Bsh1236I CGCG 1 cut(s) 558
BshFI GGCC 1 cut(s) 550
BshVI ATCGAT 2 cut(s) 105, 137
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 4 cut(s) 24, 38, 60, 68
BsmBI CGTCTC 1 cut(s) 60
BsnI GGCC 1 cut(s) 550
Bso31I GGTCTC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 129
Bsp143I GATC 4 cut(s) 134, 208, 613, 836
BspACI CCGC 2 cut(s) 206, 556
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 2 cut(s) 204, 715
BspDI ATCGAT 2 cut(s) 105, 137
BspEI TCCGGA 1 cut(s) 129
BspFNI CGCG 1 cut(s) 558
BspHI TCATGA 2 cut(s) 85, 454
BspPI GGATC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 68
BssMI GATC 4 cut(s) 134, 208, 613, 836
BssNI GRCGYC 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 390, 574
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 159, 396
BstDEI CTNAG 4 cut(s) 212, 326, 723, 840
BstF5I GGATG 2 cut(s) 232, 367
BstFNI CGCG 1 cut(s) 558
BstKTI GATC 4 cut(s) 137, 211, 616, 839
BstMAI GTCTC 4 cut(s) 24, 38, 60, 68
BstMBI GATC 4 cut(s) 134, 208, 613, 836
BstMWI GCNNNNNNNGC 1 cut(s) 773
BstNSI RCATGY 1 cut(s) 817
BstSFI CTRYAG 1 cut(s) 344
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 2 cut(s) 352, 714
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 208, 836
BstYI RGATCY 2 cut(s) 208, 836
Bsu15I ATCGAT 2 cut(s) 105, 137
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 1 cut(s) 550
BsuTUI ATCGAT 2 cut(s) 105, 137
BtgZI GCGATG 2 cut(s) 4, 100
BtsCI GGATG 2 cut(s) 232, 367
BtsI GCAGTG 2 cut(s) 168, 255
BtsIMutI CAGTG 3 cut(s) 168, 255, 801
Cac8I GCNNGC 2 cut(s) 159, 396
CaiI CAGNNNCTG 1 cut(s) 706
CciI TCATGA 2 cut(s) 85, 454
ClaI ATCGAT 2 cut(s) 105, 137
CseI GACGC 2 cut(s) 42, 83
Csp6I GTAC 1 cut(s) 323
CviAII CATG 5 cut(s) 86, 455, 479, 509, 814
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 4 cut(s) 212, 326, 723, 840
DpnI GATC 4 cut(s) 136, 210, 615, 838
DpnII GATC 4 cut(s) 134, 208, 613, 836
DrdI GACNNNNNNGTC 1 cut(s) 52
DseDI GACNNNNNNGTC 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 68
Eco53kI GAGCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 762
EcoICRI GAGCTC 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 163
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 5 cut(s) 89, 458, 482, 512, 817
FatI CATG 5 cut(s) 85, 454, 478, 508, 813
FauI CCCGC 1 cut(s) 563
Fnu4HI GCNGC 2 cut(s) 341, 728
FokI GGATG 2 cut(s) 239, 374
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 2 cut(s) 341, 728
FspBI CTAG 3 cut(s) 233, 312, 890
GluI GCNGC 2 cut(s) 341, 728
GsuI CTGGAG 2 cut(s) 768, 807
HaeIII GGCC 1 cut(s) 550
HapII CCGG 1 cut(s) 130
HgaI GACGC 2 cut(s) 42, 83
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 5 cut(s) 89, 458, 482, 512, 817
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 5 cut(s) 107, 290, 365, 853, 878
HpaII CCGG 1 cut(s) 130
HphI GGTGA 3 cut(s) 424, 520, 873
Hpy166II GTNNAC 3 cut(s) 570, 577, 819
Hpy188I TCNGA 5 cut(s) 213, 677, 690, 708, 724
Hpy188III TCNNGA 6 cut(s) 86, 130, 455, 647, 741, 824
Hpy8I GTNNAC 3 cut(s) 570, 577, 819
HpyAV CCTTC 2 cut(s) 352, 406
HpyCH4III ACNGT 2 cut(s) 390, 574
HpyCH4IV ACGT 2 cut(s) 495, 761
HpyCH4V TGCA 6 cut(s) 302, 340, 394, 542, 619, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 773
HpyF3I CTNAG 4 cut(s) 212, 326, 723, 840
HpySE526I ACGT 2 cut(s) 495, 761
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 5 cut(s) 89, 458, 482, 512, 817
Kpn2I TCCGGA 1 cut(s) 129
Kzo9I GATC 4 cut(s) 134, 208, 613, 836
LmnI GCTCC 4 cut(s) 154, 697, 787, 898
Lsp1109I GCAGC 2 cut(s) 352, 714
LweI GCATC 1 cut(s) 529
MaeI CTAG 3 cut(s) 233, 312, 890
MaeII ACGT 2 cut(s) 495, 761
MaeIII GTNAC 1 cut(s) 430
MalI GATC 4 cut(s) 136, 210, 615, 838
MboI GATC 4 cut(s) 134, 208, 613, 836
MboII GAAGA 3 cut(s) 342, 449, 915
MfeI CAATTG 1 cut(s) 620
MflI RGATCY 2 cut(s) 208, 836
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 8 cut(s) 9, 196, 334, 375, 518, 560, 620, 650
MnlI CCTC 4 cut(s) 217, 259, 687, 863
MroI TCCGGA 1 cut(s) 129
MseI TTAA 4 cut(s) 99, 237, 516, 933
MspI CCGG 1 cut(s) 130
MunI CAATTG 1 cut(s) 620
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 1 cut(s) 773
NdeII GATC 4 cut(s) 134, 208, 613, 836
NlaIII CATG 5 cut(s) 89, 458, 482, 512, 817
NmuCI GTSAC 1 cut(s) 430
NspI RCATGY 1 cut(s) 817
PagI TCATGA 2 cut(s) 85, 454
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PfeI GAWTC 5 cut(s) 107, 290, 365, 853, 878
PkrI GCNGC 2 cut(s) 342, 729
PscI ACATGT 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 706
PsuI RGATCY 2 cut(s) 208, 836
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SacI GAGCTC 1 cut(s) 163
SaqAI TTAA 4 cut(s) 99, 237, 516, 933
SatI GCNGC 2 cut(s) 341, 728
Sau3AI GATC 4 cut(s) 134, 208, 613, 836
ScaI AGTACT 1 cut(s) 324
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 162
SmoI CTYRAG 1 cut(s) 162
Sse9I AATT 8 cut(s) 9, 196, 334, 375, 518, 560, 620, 650
SsiI CCGC 2 cut(s) 206, 556
SspMI CTAG 3 cut(s) 233, 312, 890
SstI GAGCTC 1 cut(s) 163
TaaI ACNGT 2 cut(s) 390, 574
TaiI ACGT 2 cut(s) 498, 764
TaqI TCGA 3 cut(s) 105, 137, 410
TasI AATT 8 cut(s) 9, 196, 334, 375, 518, 560, 620, 650
TatI WGTACW 1 cut(s) 322
TfiI GAWTC 5 cut(s) 107, 290, 365, 853, 878
Tru1I TTAA 4 cut(s) 99, 237, 516, 933
Tru9I TTAA 4 cut(s) 99, 237, 516, 933
TscAI CASTG 3 cut(s) 175, 255, 808
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 2 cut(s) 340, 727
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 7 cut(s) 74, 102, 103, 155, 209, 443, 471
TspRI CASTG 3 cut(s) 175, 255, 808
XapI RAATTY 4 cut(s) 196, 334, 518, 560
XceI RCATGY 1 cut(s) 817
XspI CTAG 3 cut(s) 233, 312, 890
ZrmI AGTACT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.