FvH4_6g24101

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007257
Physical Location & Seq
Forward (+)
18159517 .. 18162028
2512 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24101.t1

Sequence Viewer

Length: 924 bp
ATGCCAGAAGAATCCGTACGGTCGAAGATGGAGTCGCTAAGGGTGGCCTGCGACAGAGAGATTCGAACTCACCTCCAGAAGACCGATGCTGCCACGGCGGCGCTTCACGACTCCCTTGCTACCATCCACGCCAGAACTCGAGAAACAGTCCAGTGCCAAGGGAGACTTGGAGAGGCTAAAGCGAACCTGCGACAAGCCGAGGAGGAATTGTTCAAGGCCCTAGCAGCAAAGACTCGTAAAGAGGCCAAGCGGATGGCAATGATGGATGCAATTTCTGCTAGGAAAGCTAGAATCGACGAGCTTAAGAGAGTGGTGCAGGATCGGAGGGCTAAGAGGGATGAGTATGCTGCAATTCTGAATCAAATTTCTGGATCTGAAGATAGGTCGATTGAAGACAGTAAAGAAGAAACAGAAGAGGCCATATCATGGTATAATGAGGTCCTTGGTTTCCATATTGAAGGCGGACATGGGGTGAGATTCAAGTTCAAACATATTAACTCAAAAAATCCAAATGAGGAATTCTCCTTCACTGTTAGGCTCGCAAATGATGTGTACACCTTGTTAGATTGTGATCCACACTTGAATGAGATCAAAGGGTTGTTAGACGAAGCGAACAGAACCAATGGATTATTTAAATTTGTCAGAGATATGAGACAAAGGTTTCAAGAAGCTGCTTCTCAAGGATTAGCAATGGTTCTGCATCAAGAGTATTCAACAATTTCTGGGTCTGCCCCAGCTTTGTCAGTGTCAACAGTCAGAAGTGAATCTTTTGCTGAGAGTCATGAGCAACTCCAAGTTCAACATGAAAAACCTGATAGACAAGTCAAGATATTTAGCCCTGGAAAGGGGAGTAAATCACAGAATCTATCTCCTGGGTCTTCATTGTCTCGTCGTCGCTCTCCTCGTTTTAGGGTCAGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

308

Amino Acids

34.85

Weight (kDa)

9.28

Isoelectric Point (pI)

59.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 154 - 222 4.2e-22 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 195
AccB7I CCANNNNNTGG 1 cut(s) 426
AciI CCGC 3 cut(s) 98, 250, 462
AclWI GGATC 3 cut(s) 327, 379, 566
AcsI RAATTY 3 cut(s) 363, 518, 635
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 2 cut(s) 18, 554
AfiI CCNNNNNNNGG 3 cut(s) 426, 844, 845
AflII CTTAAG 1 cut(s) 302
AgsI TTSAA 9 cut(s) 214, 392, 458, 481, 487, 583, 665, 714, 800
AjnI CCWGG 2 cut(s) 838, 871
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 4 cut(s) 287, 301, 671, 737
AluI AGCT 4 cut(s) 287, 301, 671, 737
Alw26I GTCTC 3 cut(s) 157, 646, 891
AlwI GGATC 3 cut(s) 327, 379, 566
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 4 cut(s) 45, 216, 243, 417
ApeKI GCWGC 4 cut(s) 89, 224, 347, 671
ApoI RAATTY 3 cut(s) 363, 518, 635
ArsI GACNNNNNNTTYG 2 cut(s) 17, 49
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 2 cut(s) 217, 439
AsuHPI GGTGA 2 cut(s) 62, 484
AsuII TTCGAA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 1 cut(s) 439
BarI GAAGNNNNNNTAC 1 cut(s) 32
BbsI GAAGAC 3 cut(s) 86, 399, 870
BbvI GCAGC 4 cut(s) 76, 236, 334, 658
BccI CCATC 4 cut(s) 22, 131, 247, 256
BceAI ACGGC 1 cut(s) 111
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 2 cut(s) 840, 873
BcoDI GTCTC 3 cut(s) 157, 646, 891
BfaI CTAG 3 cut(s) 221, 279, 288
BfoI RGCGCY 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 195
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 5 cut(s) 90, 99, 225, 348, 672
BlsI GCNGC 5 cut(s) 91, 100, 226, 349, 673
Bme1390I CCNGG 2 cut(s) 840, 873
Bme18I GGWCC 1 cut(s) 439
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 217, 439
BmrFI CCNGG 2 cut(s) 840, 873
BmsI GCATC 3 cut(s) 76, 256, 709
BpiI GAAGAC 3 cut(s) 86, 399, 870
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 1 cut(s) 38
Bpu14I TTCGAA 1 cut(s) 64
BpuEI CTTGAG 1 cut(s) 663
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 6 cut(s) 93, 157, 198, 442, 838, 872
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 3 cut(s) 426, 844, 845
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 2 cut(s) 264, 696
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 2 cut(s) 840, 873
BseDI CCNNGG 6 cut(s) 93, 157, 198, 442, 838, 872
BseGI GGATG 4 cut(s) 123, 258, 271, 343
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 3 cut(s) 426, 844, 845
BseMI GCAATG 2 cut(s) 264, 696
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 1 cut(s) 151
BseRI GAGGAG 2 cut(s) 215, 891
BseXI GCAGC 4 cut(s) 76, 236, 334, 658
BseYI CCCAGC 1 cut(s) 733
BsgI GTGCAG 1 cut(s) 335
Bsh1285I CGRYCG 1 cut(s) 23
BshFI GGCC 4 cut(s) 47, 218, 245, 419
BsiEI CGRYCG 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 138
BsiWI CGTACG 1 cut(s) 16
BslI CCNNNNNNNGG 3 cut(s) 426, 844, 845
BsmAI GTCTC 3 cut(s) 157, 646, 891
BsnI GGCC 4 cut(s) 47, 218, 245, 419
BsoBI CYCGRG 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 552
Bsp143I GATC 4 cut(s) 319, 371, 571, 588
BspACI CCGC 3 cut(s) 98, 250, 462
BspANI GGCC 4 cut(s) 47, 218, 245, 419
BspCNI CTCAG 1 cut(s) 766
BspHI TCATGA 1 cut(s) 781
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 3 cut(s) 327, 379, 566
BspT104I TTCGAA 1 cut(s) 64
BspTI CTTAAG 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 264, 696
BsrGI TGTACA 1 cut(s) 552
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 6 cut(s) 93, 157, 198, 442, 838, 872
BssMI GATC 4 cut(s) 319, 371, 571, 588
BssT1I CCWWGG 2 cut(s) 157, 442
Bst2UI CCWGG 2 cut(s) 840, 873
Bst4CI ACNGT 5 cut(s) 21, 148, 398, 532, 754
Bst6I CTCTTC 1 cut(s) 408
BstAFI CTTAAG 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 275
BstAUI TGTACA 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 2 cut(s) 49, 540
BstDEI CTNAG 3 cut(s) 38, 330, 774
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 4 cut(s) 123, 258, 271, 343
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstKTI GATC 4 cut(s) 322, 374, 574, 591
BstMAI GTCTC 3 cut(s) 157, 646, 891
BstMBI GATC 4 cut(s) 319, 371, 571, 588
BstMCI CGRYCG 1 cut(s) 23
BstMWI GCNNNNNNNGC 5 cut(s) 95, 98, 224, 275, 284
BstNI CCWGG 2 cut(s) 840, 873
BstSCI CCNGG 2 cut(s) 838, 871
BstV1I GCAGC 4 cut(s) 76, 236, 334, 658
BstV2I GAAGAC 3 cut(s) 86, 399, 870
BstX2I RGATCY 1 cut(s) 371
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 4 cut(s) 47, 218, 245, 419
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 4 cut(s) 123, 258, 271, 343
BtsIMutI CAGTG 3 cut(s) 158, 528, 750
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 49, 540
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 217, 439
Csp6I GTAC 2 cut(s) 17, 553
CviAII CATG 4 cut(s) 426, 467, 782, 803
CviQI GTAC 2 cut(s) 17, 553
DdeI CTNAG 3 cut(s) 38, 330, 774
DpnI GATC 4 cut(s) 321, 373, 573, 590
DpnII GATC 4 cut(s) 319, 371, 571, 588
DraI TTTAAA 1 cut(s) 634
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 157, 442
Eco47I GGWCC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 138
EcoO109I RGGNCCY 2 cut(s) 217, 439
EcoRI GAATTC 1 cut(s) 518
EcoRII CCWGG 2 cut(s) 838, 871
EcoT14I CCWWGG 2 cut(s) 157, 442
ErhI CCWWGG 2 cut(s) 157, 442
FaeI CATG 4 cut(s) 429, 470, 785, 806
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 751, 783
FatI CATG 4 cut(s) 425, 466, 781, 802
Fnu4HI GCNGC 5 cut(s) 90, 99, 225, 348, 672
FokI GGATG 4 cut(s) 110, 265, 278, 350
Fsp4HI GCNGC 5 cut(s) 90, 99, 225, 348, 672
FspBI CTAG 3 cut(s) 221, 279, 288
GlaI GCGC 1 cut(s) 102
GluI GCNGC 5 cut(s) 90, 99, 225, 348, 672
GsaI CCCAGC 1 cut(s) 737
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 4 cut(s) 47, 218, 245, 419
HhaI GCGC 1 cut(s) 103
Hin1II CATG 4 cut(s) 429, 470, 785, 806
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HphI GGTGA 2 cut(s) 62, 484
Hpy166II GTNNAC 3 cut(s) 553, 555, 750
Hpy188I TCNGA 5 cut(s) 324, 357, 376, 644, 758
Hpy188III TCNNGA 9 cut(s) 76, 107, 140, 369, 665, 704, 782, 826, 916
Hpy8I GTNNAC 3 cut(s) 553, 555, 750
Hpy99I CGWCG 3 cut(s) 299, 894, 897
HpyAV CCTTC 2 cut(s) 452, 535
HpyCH4III ACNGT 5 cut(s) 21, 148, 398, 532, 754
HpyCH4V TGCA 4 cut(s) 269, 316, 350, 700
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 98, 224, 275, 284
HpyF3I CTNAG 3 cut(s) 38, 330, 774
Hsp92II CATG 4 cut(s) 429, 470, 785, 806
HspAI GCGC 1 cut(s) 101
Kzo9I GATC 4 cut(s) 319, 371, 571, 588
Lsp1109I GCAGC 4 cut(s) 76, 236, 334, 658
LweI GCATC 3 cut(s) 76, 256, 709
MaeI CTAG 3 cut(s) 221, 279, 288
MalI GATC 4 cut(s) 321, 373, 573, 590
MboI GATC 4 cut(s) 319, 371, 571, 588
MboII GAAGA 8 cut(s) 20, 37, 91, 389, 404, 416, 425, 870
MflI RGATCY 1 cut(s) 371
MluCI AATT 7 cut(s) 206, 270, 351, 363, 518, 635, 717
MlyI GAGTC 4 cut(s) 41, 104, 226, 787
MseI TTAA 3 cut(s) 303, 495, 633
MslI CAYNNNNRTG 1 cut(s) 582
MspCI CTTAAG 1 cut(s) 302
MspR9I CCNGG 2 cut(s) 840, 873
MvaI CCWGG 2 cut(s) 840, 873
MwoI GCNNNNNNNGC 5 cut(s) 95, 98, 224, 275, 284
NdeII GATC 4 cut(s) 319, 371, 571, 588
NlaIII CATG 4 cut(s) 429, 470, 785, 806
NmeAIII GCCGAG 1 cut(s) 223
NspV TTCGAA 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 901
PfeI GAWTC 7 cut(s) 11, 61, 291, 358, 477, 764, 862
Pfl23II CGTACG 1 cut(s) 16
PflMI CCANNNNNTGG 1 cut(s) 426
PkrI GCNGC 5 cut(s) 91, 100, 226, 349, 673
PleI GAGTC 4 cut(s) 40, 104, 226, 786
PpsI GAGTC 4 cut(s) 40, 104, 226, 786
PpuMI RGGWCCY 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 2 cut(s) 838, 871
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 2 cut(s) 838, 871
PspLI CGTACG 1 cut(s) 16
PspPI GGNCC 2 cut(s) 217, 439
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 18, 554
RsaNI GTAC 2 cut(s) 17, 553
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 3 cut(s) 303, 495, 633
SatI GCNGC 5 cut(s) 90, 99, 225, 348, 672
Sau3AI GATC 4 cut(s) 319, 371, 571, 588
Sau96I GGNCC 2 cut(s) 217, 439
SchI GAGTC 4 cut(s) 41, 104, 226, 787
ScrFI CCNGG 2 cut(s) 840, 873
SfaNI GCATC 3 cut(s) 76, 256, 709
Sfr274I CTCGAG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 1 cut(s) 439
SlaI CTCGAG 1 cut(s) 138
SmiI ATTTAAAT 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 3 cut(s) 138, 302, 678
SmoI CTYRAG 3 cut(s) 138, 302, 678
Sse9I AATT 7 cut(s) 206, 270, 351, 363, 518, 635, 717
SsiI CCGC 3 cut(s) 98, 250, 462
SspMI CTAG 3 cut(s) 221, 279, 288
StyD4I CCNGG 2 cut(s) 838, 871
StyI CCWWGG 2 cut(s) 157, 442
SwaI ATTTAAAT 1 cut(s) 634
TaaI ACNGT 5 cut(s) 21, 148, 398, 532, 754
TaqI TCGA 5 cut(s) 23, 64, 139, 294, 386
TaqII GACCGA 1 cut(s) 98
TasI AATT 7 cut(s) 206, 270, 351, 363, 518, 635, 717
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 7 cut(s) 11, 61, 291, 358, 477, 764, 862
Tru1I TTAA 3 cut(s) 303, 495, 633
Tru9I TTAA 3 cut(s) 303, 495, 633
TscAI CASTG 3 cut(s) 158, 535, 750
TseI GCWGC 4 cut(s) 89, 224, 347, 671
TspDTI ATGAA 2 cut(s) 819, 870
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 3 cut(s) 158, 535, 750
Van91I CCANNNNNTGG 1 cut(s) 426
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 3 cut(s) 363, 518, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 138
XspI CTAG 3 cut(s) 221, 279, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.