MD14G1175700.v1.1

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007257
Physical Location & Seq
Reverse (-)
26910607 .. 26913492
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1175700.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGACGCCAAACCAGAGGAGTCCGTACGGACGAAGATGGAGTCGCTACGGATGGCCTGCGAGAGTGAGATTCCGATCCACCAGCAGGATTTGGATGCCTTCGCGGCGTCGTTTCGGGACTCCCTGGCTTCAGCCCGCGCCACCGCACAGGAAACCCTCCAGTCCCAAGGTAAGTTAGGAGAGGCTAAAGCTAAATTGAGGGAAGCTGAGGATGATTTGTTTAAAGCCCTAGCAGTGAAGACCCGTAAAGAGGCCAAGCAAATGGCATTGATGGAGGCCATTGAAGCTAGGAAAGCTAGAATTGAAGTACTTAAGAGAACGGTGCAAGATCAAAGGGCTAAGAGGGATGAATATGCTGCAATCCTTTCTCAGCAATCTTTTGAGTCTGAAGAAAATTGTATCGGAGACAGAAAAGATGAAATAAAAGAGGCCATCTTGTGGTATAATAGGGTCCTTGGTTTCTACATTGAAGGCGGACATGGTGTAAAATTCACATTCAAGAACATTAGTTTGAAGAATCCAGATCAGGAATTCCGTTTCACTGTTCGCCATGCAAATGATACTTACACCTTGGTAGATTGTGATCCGTACTTGAACGAGACCAAAGAGATGATTCATGAATTGAACAGAAACAATGAGTTATTTAAATTTGTCAGAGATATCAGAGTGAAGTTTCAAGAAGTTGGGGCTCAAGGATTACCAACTGTTCTTCCTCAAGATTCTTCCACAATTTCTGGGTCTGCTCCAGTTTTGTCTGTCTCAACTGGGAGAAGTGAATCTCTGGCCGAGATTTATGACCACCAAGTTCAATATGGAGAAGAAAATAGACATTTCAAGAAACCTAACCATGGGAAGGGGAGTAAAGCAGCAATTCAATCTCCTGCCTCTGCATTATCTATGCGTCGGTCTCCTCGTTTAAGGGTTAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

311

Amino Acids

35.17

Weight (kDa)

8.58

Isoelectric Point (pI)

57.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 158 - 226 1.5e-21 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 2 cut(s) 104, 138
AciI CCGC 4 cut(s) 104, 136, 144, 474
AclWI GGATC 2 cut(s) 70, 578
AcoI YGGCCR 1 cut(s) 783
AcsI RAATTY 3 cut(s) 488, 530, 647
AcuI CTGAAG 2 cut(s) 114, 408
AcyI GRCGYC 2 cut(s) 6, 107
AfaI GTAC 3 cut(s) 27, 309, 590
AfiI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
AflII CTTAAG 1 cut(s) 311
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 4 cut(s) 191, 206, 287, 296
AluI AGCT 4 cut(s) 191, 206, 287, 296
Alw26I GTCTC 4 cut(s) 399, 593, 763, 912
AlwI GGATC 2 cut(s) 70, 578
AoxI GGCC 5 cut(s) 54, 252, 276, 429, 783
ApeKI GCWGC 2 cut(s) 356, 866
ApoI RAATTY 3 cut(s) 488, 530, 647
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 451
AvaII GGWCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 245
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 2 cut(s) 343, 878
BccI CCATC 4 cut(s) 31, 46, 265, 440
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 4 cut(s) 399, 593, 763, 912
BfaI CTAG 3 cut(s) 230, 288, 297
BfrI CTTAAG 1 cut(s) 311
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 3 cut(s) 105, 357, 867
BlsI GCNGC 3 cut(s) 106, 358, 868
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 451
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 774
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 774
BpiI GAAGAC 1 cut(s) 245
BpmI CTGGAG 2 cut(s) 143, 729
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 675, 699
BsaBI GATNNNNATC 2 cut(s) 74, 582
BsaHI GRCGYC 2 cut(s) 6, 107
BsaI GGTCTC 2 cut(s) 593, 912
BsaJI CCNNGG 5 cut(s) 123, 166, 454, 570, 847
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
Bse1I ACTGG 3 cut(s) 160, 746, 769
Bse8I GATNNNNATC 2 cut(s) 74, 582
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 5 cut(s) 123, 166, 454, 570, 847
BseGI GGATG 4 cut(s) 57, 100, 217, 352
BseJI GATNNNNATC 2 cut(s) 74, 582
BseLI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
BseMII CTCAG 2 cut(s) 198, 383
BseNI ACTGG 3 cut(s) 160, 746, 769
BseRI GAGGAG 2 cut(s) 32, 900
BseXI GCAGC 2 cut(s) 343, 878
Bsh1236I CGCG 2 cut(s) 104, 138
BshFI GGCC 5 cut(s) 56, 254, 278, 431, 785
BsiWI CGTACG 1 cut(s) 25
BslFI GGGAC 2 cut(s) 131, 148
BslI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
BsmAI GTCTC 4 cut(s) 399, 593, 763, 912
BsmFI GGGAC 2 cut(s) 131, 148
BsnI GGCC 5 cut(s) 56, 254, 278, 431, 785
Bso31I GGTCTC 2 cut(s) 593, 912
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 4 cut(s) 75, 328, 523, 583
Bsp19I CCATGG 1 cut(s) 847
BspACI CCGC 4 cut(s) 104, 136, 144, 474
BspANI GGCC 5 cut(s) 56, 254, 278, 431, 785
BspCNI CTCAG 2 cut(s) 199, 382
BspFNI CGCG 2 cut(s) 104, 138
BspHI TCATGA 1 cut(s) 616
BspLI GGNNCC 1 cut(s) 452
BspPI GGATC 2 cut(s) 70, 578
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 593, 912
BsrI ACTGG 3 cut(s) 160, 746, 769
BssECI CCNNGG 5 cut(s) 123, 166, 454, 570, 847
BssMI GATC 4 cut(s) 75, 328, 523, 583
BssNI GRCGYC 2 cut(s) 6, 107
BssT1I CCWWGG 4 cut(s) 166, 454, 570, 847
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 322, 544, 706
BstACI GRCGYC 2 cut(s) 6, 107
BstAFI CTTAAG 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 58, 136
BstDEI CTNAG 3 cut(s) 207, 339, 369
BstDSI CCRYGG 1 cut(s) 847
BstF5I GGATG 4 cut(s) 57, 100, 217, 352
BstFNI CGCG 2 cut(s) 104, 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 78, 331, 526, 586
BstMAI GTCTC 4 cut(s) 399, 593, 763, 912
BstMBI GATC 4 cut(s) 75, 328, 523, 583
BstMWI GCNNNNNNNGC 3 cut(s) 104, 284, 293
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstUI CGCG 2 cut(s) 104, 138
BstV1I GCAGC 2 cut(s) 343, 878
BstV2I GAAGAC 1 cut(s) 245
BstXI CCANNNNNNTGG 1 cut(s) 262
BsuRI GGCC 5 cut(s) 56, 254, 278, 431, 785
BtgI CCRYGG 1 cut(s) 847
BtsCI GGATG 4 cut(s) 57, 100, 217, 352
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 240, 540
Cac8I GCNNGC 2 cut(s) 58, 136
CciI TCATGA 1 cut(s) 616
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 451
CseI GACGC 3 cut(s) 14, 96, 890
Csp6I GTAC 3 cut(s) 26, 308, 589
CviAII CATG 4 cut(s) 479, 551, 617, 848
CviQI GTAC 3 cut(s) 26, 308, 589
DdeI CTNAG 3 cut(s) 207, 339, 369
DpnI GATC 4 cut(s) 77, 330, 525, 585
DpnII GATC 4 cut(s) 75, 328, 523, 583
DraI TTTAAA 2 cut(s) 223, 646
EaeI YGGCCR 1 cut(s) 783
EciI GGCGGA 1 cut(s) 489
Eco130I CCWWGG 4 cut(s) 166, 454, 570, 847
Eco24I GRGCYC 1 cut(s) 691
Eco31I GGTCTC 2 cut(s) 593, 912
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 114, 408
EcoO109I RGGNCCY 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 4 cut(s) 166, 454, 570, 847
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 4 cut(s) 166, 454, 570, 847
FaeI CATG 4 cut(s) 482, 554, 620, 851
FaiI YATR 9 cut(s) 354, 444, 480, 552, 618, 795, 813, 849, 899
FaqI GGGAC 2 cut(s) 131, 148
FatI CATG 4 cut(s) 478, 550, 616, 847
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 3 cut(s) 105, 357, 867
FokI GGATG 4 cut(s) 64, 107, 224, 359
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 3 cut(s) 105, 357, 867
FspBI CTAG 3 cut(s) 230, 288, 297
GlaI GCGC 1 cut(s) 139
GluI GCNGC 3 cut(s) 105, 357, 867
GsuI CTGGAG 2 cut(s) 143, 729
HaeIII GGCC 5 cut(s) 56, 254, 278, 431, 785
HgaI GACGC 3 cut(s) 14, 96, 890
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 2 cut(s) 6, 107
Hin1II CATG 4 cut(s) 482, 554, 620, 851
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 9 cut(s) 20, 41, 70, 119, 383, 517, 613, 719, 776
Hpy188I TCNGA 5 cut(s) 75, 388, 404, 656, 665
Hpy188III TCNNGA 8 cut(s) 116, 499, 521, 527, 617, 677, 716, 835
Hpy99I CGWCG 2 cut(s) 112, 906
HpyAV CCTTC 3 cut(s) 109, 464, 847
HpyCH4III ACNGT 3 cut(s) 322, 544, 706
HpyCH4V TGCA 4 cut(s) 325, 359, 554, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 284, 293
HpyF3I CTNAG 3 cut(s) 207, 339, 369
Hsp92I GRCGYC 2 cut(s) 6, 107
Hsp92II CATG 4 cut(s) 482, 554, 620, 851
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 4 cut(s) 75, 328, 523, 583
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 2 cut(s) 343, 878
LweI GCATC 1 cut(s) 85
MaeI CTAG 3 cut(s) 230, 288, 297
MalI GATC 4 cut(s) 77, 330, 525, 585
MboI GATC 4 cut(s) 75, 328, 523, 583
MboII GAAGA 7 cut(s) 46, 250, 401, 526, 701, 714, 830
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 9 cut(s) 194, 300, 394, 488, 530, 620, 647, 729, 870
MlyI GAGTC 4 cut(s) 29, 50, 113, 392
MseI TTAA 5 cut(s) 222, 312, 645, 917, 924
MslI CAYNNNNRTG 1 cut(s) 555
MspCI CTTAAG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 2 cut(s) 104, 138
MwoI GCNNNNNNNGC 3 cut(s) 104, 284, 293
NcoI CCATGG 1 cut(s) 847
NdeII GATC 4 cut(s) 75, 328, 523, 583
NlaIII CATG 4 cut(s) 482, 554, 620, 851
NlaIV GGNNCC 1 cut(s) 452
NmeAIII GCCGAG 1 cut(s) 811
PagI TCATGA 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 910
PfeI GAWTC 5 cut(s) 70, 517, 613, 719, 776
Pfl23II CGTACG 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 3 cut(s) 106, 358, 868
PleI GAGTC 4 cut(s) 28, 49, 113, 391
PpsI GAGTC 4 cut(s) 28, 49, 113, 391
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspLI CGTACG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 1 cut(s) 451
PspPPI RGGWCCY 1 cut(s) 451
RsaI GTAC 3 cut(s) 27, 309, 590
RsaNI GTAC 3 cut(s) 26, 308, 589
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 5 cut(s) 222, 312, 645, 917, 924
SatI GCNGC 3 cut(s) 105, 357, 867
Sau3AI GATC 4 cut(s) 75, 328, 523, 583
Sau96I GGNCC 1 cut(s) 451
ScaI AGTACT 1 cut(s) 309
SchI GAGTC 4 cut(s) 29, 50, 113, 392
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 691
SetI ASST 7 cut(s) 172, 193, 208, 289, 298, 572, 844
SfaNI GCATC 1 cut(s) 85
SinI GGWCC 1 cut(s) 451
SmiI ATTTAAAT 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 3 cut(s) 311, 690, 714
SmoI CTYRAG 3 cut(s) 311, 690, 714
Sse9I AATT 9 cut(s) 194, 300, 394, 488, 530, 620, 647, 729, 870
SsiI CCGC 4 cut(s) 104, 136, 144, 474
SspMI CTAG 3 cut(s) 230, 288, 297
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 4 cut(s) 166, 454, 570, 847
SwaI ATTTAAAT 1 cut(s) 646
TaaI ACNGT 3 cut(s) 322, 544, 706
TaqII GACCGA 1 cut(s) 894
TasI AATT 9 cut(s) 194, 300, 394, 488, 530, 620, 647, 729, 870
TatI WGTACW 1 cut(s) 307
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 5 cut(s) 70, 517, 613, 719, 776
Tru1I TTAA 5 cut(s) 222, 312, 645, 917, 924
Tru9I TTAA 5 cut(s) 222, 312, 645, 917, 924
TscAI CASTG 2 cut(s) 240, 547
TseI GCWGC 2 cut(s) 356, 866
TspDTI ATGAA 4 cut(s) 363, 432, 605, 633
TspGWI ACGGA 5 cut(s) 13, 43, 64, 524, 576
TspRI CASTG 2 cut(s) 240, 547
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 451
XapI RAATTY 3 cut(s) 488, 530, 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 809
XspI CTAG 3 cut(s) 230, 288, 297
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.