Rh7AG132200

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007257
Physical Location & Seq
Reverse (-)
10588443 .. 10591073
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG132200.1

Sequence Viewer

Length: 675 bp
ATGGCAATGACGGATGCGATTGCTGCCAGGAAAGCTAGAATAGACGAACTTAAGAGAGTGGTGCAAGATCGAAGGGCTAAGAGGGATGAGTATGGTGCAATTCTTTCTCAGCAATTTTCTGAATCTGAAGATAGGTCTATCGAAGACAGTAAAGAAGAAATAGAAGAGGCCATATCATGGTATAATAAGGTCCTTGGTTTCTATATTGAAGGCGGACATGGGGTGAGATTCACATTTAAGAACATTAACTTGAAAAATCCAGATGAGGAATTCTGCTTCACTGTTAGGCATGCAAATGATGTGTACACCTTGATAGATTGTGATCCACACTTGAATGAGATCAAAGAGTTGTTAGACGAATTGAACAGAACCAATGGATTATTTAAATTTGTCAGAGATATGAGACAAAGGTTTCAAGAAGCTGCCTCTAGAGGGTTACCAATGGTTATGCATCAAGAGTATTCCACAATTTCTGGGTCTGCTCCAGCTTTGTCAATGTCAACTGTCAGAAGTGAATCTTTTGCCAAGAGTCATGAGCAACACCAAGTTCAACATGAAAAACCTGATAGACAAGTCAAGAAACTTAGCCCTGGAAAGTGGAATAAATCGCAAAGTCTATCTCCTGGGTCGGCATTGTCTCTTCGTCGCTCTCCTCGTTTTAAGGTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

224

Amino Acids

25.85

Weight (kDa)

8.98

Isoelectric Point (pI)

64.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 71 - 139 1.9e-23 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 177
AciI CCGC 1 cut(s) 213
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 2 cut(s) 269, 386
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 177, 432
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 6 cut(s) 209, 253, 334, 364, 416, 551
AjnI CCWGG 3 cut(s) 26, 589, 622
AluBI AGCT 3 cut(s) 35, 422, 488
AluI AGCT 3 cut(s) 35, 422, 488
Alw26I GTCTC 2 cut(s) 397, 642
AlwI GGATC 1 cut(s) 317
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 23, 422
ApoI RAATTY 2 cut(s) 269, 386
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 10, 409
BciT130I CCWGG 3 cut(s) 28, 591, 624
BcoDI GTCTC 2 cut(s) 397, 642
BfaI CTAG 2 cut(s) 36, 429
BfrI CTTAAG 1 cut(s) 50
BisI GCNGC 2 cut(s) 24, 423
BlsI GCNGC 2 cut(s) 25, 424
Bme1390I CCNGG 3 cut(s) 28, 591, 624
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 3 cut(s) 28, 591, 624
BmsI GCATC 2 cut(s) 4, 460
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 3 cut(s) 193, 589, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 432
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 3 cut(s) 28, 591, 624
BseDI CCNNGG 3 cut(s) 193, 589, 623
BseGI GGATG 2 cut(s) 19, 91
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 2 cut(s) 177, 432
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 2 cut(s) 10, 409
BshFI GGCC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 177, 432
BsmAI GTCTC 2 cut(s) 397, 642
BsnI GGCC 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 3 cut(s) 67, 322, 339
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 532
BspPI GGATC 1 cut(s) 317
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 303
BssECI CCNNGG 3 cut(s) 193, 589, 623
BssMI GATC 3 cut(s) 67, 322, 339
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 3 cut(s) 28, 591, 624
Bst4CI ACNGT 3 cut(s) 149, 283, 505
Bst6I CTCTTC 2 cut(s) 159, 645
BstAFI CTTAAG 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 3 cut(s) 78, 108, 584
BstEII GGTNACC 1 cut(s) 435
BstF5I GGATG 2 cut(s) 19, 91
BstKTI GATC 3 cut(s) 70, 325, 342
BstMAI GTCTC 2 cut(s) 397, 642
BstMBI GATC 3 cut(s) 67, 322, 339
BstMWI GCNNNNNNNGC 2 cut(s) 23, 32
BstNI CCWGG 3 cut(s) 28, 591, 624
BstNSI RCATGY 1 cut(s) 293
BstPI GGTNACC 1 cut(s) 435
BstSCI CCNGG 3 cut(s) 26, 589, 622
BstV1I GCAGC 2 cut(s) 10, 409
BstV2I GAAGAC 1 cut(s) 150
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 2 cut(s) 19, 91
BtsIMutI CAGTG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 304
CviAII CATG 5 cut(s) 177, 218, 290, 533, 554
CviJI RGCY 6 cut(s) 35, 77, 170, 422, 488, 588
CviKI_1 RGCY 6 cut(s) 35, 77, 170, 422, 488, 588
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 3 cut(s) 78, 108, 584
DpnI GATC 3 cut(s) 69, 324, 341
DpnII GATC 3 cut(s) 67, 322, 339
DraI TTTAAA 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 159, 645
EarI CTCTTC 2 cut(s) 159, 645
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 147
Eco91I GGTNACC 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 190
EcoO65I GGTNACC 1 cut(s) 435
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 3 cut(s) 26, 589, 622
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 5 cut(s) 180, 221, 293, 536, 557
FalI AAGNNNNNCTT 2 cut(s) 502, 534
FatI CATG 5 cut(s) 176, 217, 289, 532, 553
Fnu4HI GCNGC 2 cut(s) 24, 423
FokI GGATG 2 cut(s) 26, 98
Fsp4HI GCNGC 2 cut(s) 24, 423
FspBI CTAG 2 cut(s) 36, 429
GluI GCNGC 2 cut(s) 24, 423
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 1 cut(s) 170
Hin1II CATG 5 cut(s) 180, 221, 293, 536, 557
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 4 cut(s) 122, 228, 515, 529
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 304, 306, 501
Hpy188I TCNGA 4 cut(s) 121, 127, 395, 509
Hpy188III TCNNGA 7 cut(s) 260, 416, 429, 455, 533, 577, 667
Hpy8I GTNNAC 3 cut(s) 304, 306, 501
Hpy99I CGWCG 1 cut(s) 648
HpyAV CCTTC 2 cut(s) 66, 203
HpyCH4III ACNGT 3 cut(s) 149, 283, 505
HpyCH4V TGCA 4 cut(s) 64, 98, 293, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 32
HpyF3I CTNAG 3 cut(s) 78, 108, 584
Hsp92II CATG 5 cut(s) 180, 221, 293, 536, 557
Kzo9I GATC 3 cut(s) 67, 322, 339
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 2 cut(s) 10, 409
LweI GCATC 2 cut(s) 4, 460
MaeI CTAG 2 cut(s) 36, 429
MaeIII GTNAC 1 cut(s) 435
MalI GATC 3 cut(s) 69, 324, 341
MboI GATC 3 cut(s) 67, 322, 339
MboII GAAGA 5 cut(s) 140, 155, 167, 176, 632
MluCI AATT 6 cut(s) 99, 113, 269, 359, 386, 468
MlyI GAGTC 1 cut(s) 538
MnlI CCTC 6 cut(s) 75, 160, 259, 425, 436, 663
Mph1103I ATGCAT 1 cut(s) 453
MseI TTAA 5 cut(s) 51, 237, 246, 384, 660
MslI CAYNNNNRTG 2 cut(s) 294, 333
MspCI CTTAAG 1 cut(s) 50
MspR9I CCNGG 3 cut(s) 28, 591, 624
MvaI CCWGG 3 cut(s) 28, 591, 624
MwoI GCNNNNNNNGC 2 cut(s) 23, 32
NdeII GATC 3 cut(s) 67, 322, 339
NlaIII CATG 5 cut(s) 180, 221, 293, 536, 557
NsiI ATGCAT 1 cut(s) 453
NspI RCATGY 1 cut(s) 293
PaeI GCATGC 1 cut(s) 293
PagI TCATGA 1 cut(s) 532
PcsI WCGNNNNNNNCGW 1 cut(s) 652
PfeI GAWTC 3 cut(s) 122, 228, 515
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 2 cut(s) 25, 424
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PpuMI RGGWCCY 1 cut(s) 190
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 26, 589, 622
PspEI GGTNACC 1 cut(s) 435
PspGI CCWGG 3 cut(s) 26, 589, 622
PspPI GGNCC 1 cut(s) 190
PspPPI RGGWCCY 1 cut(s) 190
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 294, 333
SaqAI TTAA 5 cut(s) 51, 237, 246, 384, 660
SatI GCNGC 2 cut(s) 24, 423
Sau3AI GATC 3 cut(s) 67, 322, 339
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 3 cut(s) 28, 591, 624
SetI ASST 9 cut(s) 37, 137, 192, 311, 413, 424, 490, 565, 666
SfaNI GCATC 2 cut(s) 4, 460
SinI GGWCC 1 cut(s) 190
SmiI ATTTAAAT 1 cut(s) 385
SmiMI CAYNNNNRTG 2 cut(s) 294, 333
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SphI GCATGC 1 cut(s) 293
Sse9I AATT 6 cut(s) 99, 113, 269, 359, 386, 468
SsiI CCGC 1 cut(s) 213
SspMI CTAG 2 cut(s) 36, 429
StyD4I CCNGG 3 cut(s) 26, 589, 622
StyI CCWWGG 1 cut(s) 193
SwaI ATTTAAAT 1 cut(s) 385
TaaI ACNGT 3 cut(s) 149, 283, 505
TaqI TCGA 2 cut(s) 70, 141
TasI AATT 6 cut(s) 99, 113, 269, 359, 386, 468
TatI WGTACW 1 cut(s) 303
TfiI GAWTC 3 cut(s) 122, 228, 515
Tru1I TTAA 5 cut(s) 51, 237, 246, 384, 660
Tru9I TTAA 5 cut(s) 51, 237, 246, 384, 660
TscAI CASTG 1 cut(s) 286
TseI GCWGC 2 cut(s) 23, 422
TspDTI ATGAA 1 cut(s) 570
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 286
Van91I CCANNNNNTGG 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 190
XapI RAATTY 2 cut(s) 269, 386
XbaI TCTAGA 1 cut(s) 428
XceI RCATGY 1 cut(s) 293
XspI CTAG 2 cut(s) 36, 429
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.