RLG00000004302

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007257
Physical Location & Seq
Forward (+)
58124827 .. 58126880
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004302

Sequence Viewer

Length: 936 bp
ATGGACACCAAACCAGAAGAATCCGTACGGTCGAAAATGGAGTCTCTAAAGCTGGCCTGCGACAGAGAGATTCCGGCTCACCTGCAGAAGACGGACGCTGCCGCGGCGGCGCTTCGCGACTCCCTGGCTTCGATCCGCGCCAGAGTACAAGAAACCGTCCACTGCCAAGGGAAACTCGGAGAGGCTAAAGCTAAACTGAGGGAAGCCGAGGAGGAATTGTTTAAAGCCCTAGCAGCAAAGACTCGTAAAGAGACCAAGCGGATGGCAATGACGGATGCGATTGCTGCCAGGAAAGCTAGAATAGACGAACTTAAGAGAGTGGTGCAAGATCGAAGGGCTAAGAGGGATGAGTATGGTGCAATTCTTTCTCAGCAATTTTCTGAATCTGAAGATAGGTCTATCGAAGACAGTAAAGAAGAATTAGAAGAGGCCATATCATGGTATAATAAGGTCCTTGGTTTCTATATTGAAGGCGGACATGGGGTGAGATTCACATTTAAGAACATCAACTTGAAAAATCCAGATGAGGAATTCTCCTTCACTGTTAGGCATGCAAATGATGTGTACACCTTGATAGATTGTGATCCACACTTGAATGAGATCAAAGAGTTGTTAGATGAATTGAACAGAACCAATGGATTATTTAAATTTGTCAGAGATATGAGACAAAGGTTTCAAGAGGCTGCCTCTCGAGGGCTACCAATGGTTATGCATCAAGAGTATTCCACAATTTCTGGGTCTGCTCCAGCTTTGTCAATGTCAACGGTCAGAAGTGAATCTTTTGCCAAGAGTCATGAGCAACACCAAGTTCAACATGAAAAACCTGATAGACAAGTCAAGAAACTTAGCCCTGGAAAGTGGAATAAATCACAAAGTCTATCTCCTGGGTCGGCATTGTCTCTTCGTCGCTCTCCTCGTTTTAAGGTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

312

Amino Acids

35.51

Weight (kDa)

9.24

Isoelectric Point (pI)

58.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 158 - 226 6.7e-24 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 90
Acc36I ACCTGC 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 3 cut(s) 104, 117, 138
AciI CCGC 6 cut(s) 102, 104, 107, 136, 259, 474
AclWI GGATC 2 cut(s) 127, 578
AcsI RAATTY 2 cut(s) 530, 647
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 3 cut(s) 27, 147, 566
AfiI CCNNNNNNNGG 2 cut(s) 438, 693
AflII CTTAAG 1 cut(s) 311
AgsI TTSAA 6 cut(s) 470, 514, 595, 625, 677, 812
AjnI CCWGG 4 cut(s) 123, 287, 850, 883
AluBI AGCT 4 cut(s) 52, 191, 296, 749
AluI AGCT 4 cut(s) 52, 191, 296, 749
Alw26I GTCTC 4 cut(s) 48, 245, 658, 903
AlwI GGATC 2 cut(s) 127, 578
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 2 cut(s) 54, 429
ApeKI GCWGC 4 cut(s) 98, 233, 284, 683
ApoI RAATTY 2 cut(s) 530, 647
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 2 cut(s) 112, 140
AspS9I GGNCC 1 cut(s) 451
AsuHPI GGTGA 2 cut(s) 71, 496
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 1 cut(s) 451
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 2 cut(s) 95, 411
BbvI GCAGC 4 cut(s) 85, 245, 271, 670
BccI CCATC 1 cut(s) 256
BciT130I CCWGG 4 cut(s) 125, 289, 852, 885
BcoDI GTCTC 4 cut(s) 48, 245, 658, 903
BfaI CTAG 2 cut(s) 230, 297
BfmI CTRYAG 1 cut(s) 83
BfoI RGCGCY 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 7 cut(s) 99, 102, 105, 108, 234, 285, 684
BlsI GCNGC 7 cut(s) 100, 103, 106, 109, 235, 286, 685
Bme1390I CCNGG 4 cut(s) 125, 289, 852, 885
Bme18I GGWCC 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 451
BmrFI CCNGG 4 cut(s) 125, 289, 852, 885
BmsI GCATC 2 cut(s) 265, 721
BpiI GAAGAC 2 cut(s) 95, 411
BplI GAGNNNNNCTC 4 cut(s) 518, 550, 671, 703
BpmI CTGGAG 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 582
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 7 cut(s) 102, 123, 166, 207, 454, 850, 884
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 693
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 4 cut(s) 125, 289, 852, 885
BseDI CCNNGG 7 cut(s) 102, 123, 166, 207, 454, 850, 884
BseGI GGATG 3 cut(s) 267, 280, 352
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 438, 693
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 188, 383
BseRI GAGGAG 2 cut(s) 224, 903
BseXI GCAGC 4 cut(s) 85, 245, 271, 670
Bsh1236I CGCG 3 cut(s) 104, 117, 138
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 2 cut(s) 56, 431
BsiEI CGRYCG 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 1 cut(s) 74
BsiWI CGTACG 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 438, 693
BsmAI GTCTC 4 cut(s) 48, 245, 658, 903
BsnI GGCC 2 cut(s) 56, 431
Bso31I GGTCTC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 690
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 4 cut(s) 132, 328, 583, 600
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 6 cut(s) 102, 104, 107, 136, 259, 474
BspANI GGCC 2 cut(s) 56, 431
BspCNI CTCAG 2 cut(s) 189, 382
BspFNI CGCG 3 cut(s) 104, 117, 138
BspHI TCATGA 1 cut(s) 793
BspMAI CTGCAG 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 90
BspPI GGATC 2 cut(s) 127, 578
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 564
BssECI CCNNGG 7 cut(s) 102, 123, 166, 207, 454, 850, 884
BssMI GATC 4 cut(s) 132, 328, 583, 600
BssT1I CCWWGG 2 cut(s) 166, 454
Bst2UI CCWGG 4 cut(s) 125, 289, 852, 885
Bst4CI ACNGT 5 cut(s) 30, 157, 410, 544, 766
Bst6I CTCTTC 2 cut(s) 420, 906
BstAFI CTTAAG 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 3 cut(s) 54, 58, 552
BstDEI CTNAG 4 cut(s) 197, 339, 369, 845
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 3 cut(s) 267, 280, 352
BstFNI CGCG 3 cut(s) 104, 117, 138
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 2 cut(s) 112, 140
BstKTI GATC 4 cut(s) 135, 331, 586, 603
BstMAI GTCTC 4 cut(s) 48, 245, 658, 903
BstMBI GATC 4 cut(s) 132, 328, 583, 600
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
BstNI CCWGG 4 cut(s) 125, 289, 852, 885
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 123, 287, 850, 883
BstSFI CTRYAG 1 cut(s) 83
BstUI CGCG 3 cut(s) 104, 117, 138
BstV1I GCAGC 4 cut(s) 85, 245, 271, 670
BstV2I GAAGAC 2 cut(s) 95, 411
BstXI CCANNNNNNTGG 1 cut(s) 262
BsuRI GGCC 2 cut(s) 56, 431
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 267, 280, 352
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 540
BtuMI TCGCGA 1 cut(s) 117
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 3 cut(s) 54, 58, 552
CciI TCATGA 1 cut(s) 793
CfoI GCGC 2 cut(s) 112, 140
Cfr13I GGNCC 1 cut(s) 451
Cfr42I CCGCGG 1 cut(s) 105
CseI GACGC 1 cut(s) 104
Csp6I GTAC 3 cut(s) 26, 146, 565
CviAII CATG 5 cut(s) 438, 479, 551, 794, 815
CviQI GTAC 3 cut(s) 26, 146, 565
DdeI CTNAG 4 cut(s) 197, 339, 369, 845
DpnI GATC 4 cut(s) 134, 330, 585, 602
DpnII GATC 4 cut(s) 132, 328, 583, 600
DraI TTTAAA 2 cut(s) 223, 646
Eam1104I CTCTTC 2 cut(s) 420, 906
EarI CTCTTC 2 cut(s) 420, 906
EciI GGCGGA 1 cut(s) 489
Eco130I CCWWGG 2 cut(s) 166, 454
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 690
EcoO109I RGGNCCY 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 4 cut(s) 123, 287, 850, 883
EcoT14I CCWWGG 2 cut(s) 166, 454
EcoT22I ATGCAT 1 cut(s) 714
ErhI CCWWGG 2 cut(s) 166, 454
FaeI CATG 5 cut(s) 441, 482, 554, 797, 818
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FatI CATG 5 cut(s) 437, 478, 550, 793, 814
Fnu4HI GCNGC 7 cut(s) 99, 102, 105, 108, 234, 285, 684
FokI GGATG 3 cut(s) 274, 287, 359
Fsp4HI GCNGC 7 cut(s) 99, 102, 105, 108, 234, 285, 684
FspBI CTAG 2 cut(s) 230, 297
GlaI GCGC 2 cut(s) 111, 139
GluI GCNGC 7 cut(s) 99, 102, 105, 108, 234, 285, 684
GsuI CTGGAG 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 2 cut(s) 56, 431
HapII CCGG 1 cut(s) 74
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 112, 140
Hin1II CATG 5 cut(s) 441, 482, 554, 797, 818
Hin6I GCGC 2 cut(s) 110, 138
HinP1I GCGC 2 cut(s) 110, 138
HincII GTYRAC 1 cut(s) 762
HindII GTYRAC 1 cut(s) 762
HinfI GANTC 9 cut(s) 20, 41, 70, 119, 241, 383, 489, 776, 790
HpaII CCGG 1 cut(s) 74
HphI GGTGA 2 cut(s) 71, 496
Hpy166II GTNNAC 4 cut(s) 160, 565, 567, 762
Hpy188I TCNGA 5 cut(s) 179, 382, 388, 656, 770
Hpy188III TCNNGA 8 cut(s) 116, 521, 677, 690, 716, 794, 838, 928
Hpy8I GTNNAC 4 cut(s) 160, 565, 567, 762
Hpy99I CGWCG 1 cut(s) 909
HpyAV CCTTC 3 cut(s) 327, 464, 547
HpyCH4III ACNGT 5 cut(s) 30, 157, 410, 544, 766
HpyCH4V TGCA 5 cut(s) 85, 325, 359, 554, 712
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
HpyF3I CTNAG 4 cut(s) 197, 339, 369, 845
Hsp92II CATG 5 cut(s) 441, 482, 554, 797, 818
HspAI GCGC 2 cut(s) 110, 138
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 4 cut(s) 132, 328, 583, 600
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 4 cut(s) 85, 245, 271, 670
LweI GCATC 2 cut(s) 265, 721
MaeI CTAG 2 cut(s) 230, 297
MalI GATC 4 cut(s) 134, 330, 585, 602
MboI GATC 4 cut(s) 132, 328, 583, 600
MboII GAAGA 7 cut(s) 29, 100, 401, 416, 428, 437, 893
MluCI AATT 8 cut(s) 215, 360, 374, 419, 530, 620, 647, 729
MlyI GAGTC 4 cut(s) 50, 113, 235, 799
Mph1103I ATGCAT 1 cut(s) 714
MseI TTAA 5 cut(s) 222, 312, 498, 645, 921
MslI CAYNNNNRTG 2 cut(s) 555, 594
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 4 cut(s) 125, 289, 852, 885
MvaI CCWGG 4 cut(s) 125, 289, 852, 885
MvnI CGCG 3 cut(s) 104, 117, 138
MwoI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
NdeII GATC 4 cut(s) 132, 328, 583, 600
NlaIII CATG 5 cut(s) 441, 482, 554, 797, 818
NmeAIII GCCGAG 1 cut(s) 232
NruI TCGCGA 1 cut(s) 117
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 1 cut(s) 554
PaeI GCATGC 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 690
PagI TCATGA 1 cut(s) 793
PaqCI CACCTGC 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PfeI GAWTC 5 cut(s) 20, 70, 383, 489, 776
Pfl23II CGTACG 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 7 cut(s) 100, 103, 106, 109, 235, 286, 685
PleI GAGTC 4 cut(s) 49, 113, 235, 798
PpsI GAGTC 4 cut(s) 49, 113, 235, 798
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 4 cut(s) 123, 287, 850, 883
PspGI CCWGG 4 cut(s) 123, 287, 850, 883
PspLI CGTACG 1 cut(s) 25
PspPI GGNCC 1 cut(s) 451
PspPPI RGGWCCY 1 cut(s) 451
PstI CTGCAG 1 cut(s) 87
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 3 cut(s) 27, 147, 566
RsaNI GTAC 3 cut(s) 26, 146, 565
RseI CAYNNNNRTG 2 cut(s) 555, 594
SacII CCGCGG 1 cut(s) 105
SaqAI TTAA 5 cut(s) 222, 312, 498, 645, 921
SatI GCNGC 7 cut(s) 99, 102, 105, 108, 234, 285, 684
Sau3AI GATC 4 cut(s) 132, 328, 583, 600
Sau96I GGNCC 1 cut(s) 451
SchI GAGTC 4 cut(s) 50, 113, 235, 799
ScrFI CCNGG 4 cut(s) 125, 289, 852, 885
SfaNI GCATC 2 cut(s) 265, 721
SfcI CTRYAG 1 cut(s) 83
Sfr274I CTCGAG 1 cut(s) 690
Sfr303I CCGCGG 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 105
SinI GGWCC 1 cut(s) 451
SlaI CTCGAG 1 cut(s) 690
SmiI ATTTAAAT 1 cut(s) 646
SmiMI CAYNNNNRTG 2 cut(s) 555, 594
SmlI CTYRAG 2 cut(s) 311, 690
SmoI CTYRAG 2 cut(s) 311, 690
SphI GCATGC 1 cut(s) 554
Sse9I AATT 8 cut(s) 215, 360, 374, 419, 530, 620, 647, 729
SsiI CCGC 6 cut(s) 102, 104, 107, 136, 259, 474
SspMI CTAG 2 cut(s) 230, 297
StyD4I CCNGG 4 cut(s) 123, 287, 850, 883
StyI CCWWGG 2 cut(s) 166, 454
SwaI ATTTAAAT 1 cut(s) 646
TaaI ACNGT 5 cut(s) 30, 157, 410, 544, 766
TaqI TCGA 5 cut(s) 32, 131, 331, 402, 691
TasI AATT 8 cut(s) 215, 360, 374, 419, 530, 620, 647, 729
TatI WGTACW 2 cut(s) 145, 564
TauI GCSGC 3 cut(s) 104, 107, 110
TfiI GAWTC 5 cut(s) 20, 70, 383, 489, 776
Tru1I TTAA 5 cut(s) 222, 312, 498, 645, 921
Tru9I TTAA 5 cut(s) 222, 312, 498, 645, 921
TscAI CASTG 2 cut(s) 167, 547
TseI GCWGC 4 cut(s) 98, 233, 284, 683
TspDTI ATGAA 2 cut(s) 633, 831
TspGWI ACGGA 3 cut(s) 13, 107, 287
TspRI CASTG 2 cut(s) 167, 547
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 451
XapI RAATTY 2 cut(s) 530, 647
XceI RCATGY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 690
XspI CTAG 2 cut(s) 230, 297
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.