MD06G1170100.v1.1

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007257
Physical Location & Seq
Reverse (-)
31069808 .. 31072438
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1170100.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGACGGCAAACCGGAGGAGTCCGTACGGACGAAGATGGAGTCGCTACGGATGGCCTGCGAGAGTGAGATTCCGATCCACGAGAAGGATTTGGATGCCTTCACGGCGTCGTTTCGGGATTCCCTGGCTTCCGTCCGCGCTACCGCACAAGAAACCCTCCAGTCCCAAGGTAAATTAGGAGAGGCTAAAGCTAAATTGAGGGAAGCTGAGGATGATTTGTTTAAAGCCCTAGCAGTGAAGACCCGTAAAGAGGCCAAGCGAATGGCATTGATGGAGGCCATTGAAGCTAGGAAAGCTAGAATTGAAGTACTTAAGAGAACAGTGCAAGATCAAAGGGCTAAGAGGGAAGAATATGCTGCAATCCTTTCTCAGCAATATTTCGAATCTGAAGAAAATTGTATCCCAGACAGAAAGGATGAAATAAAAGAGGCCATCTCTTGGTATAATAGGGTTCTTGGTTTCTACGTTGAAGGCGGACATGGTGTAAAATTCACATTCAAAAACATTAGTTTGAAGAATCCAGATCAGGAATTCTGTTTCACTGTTCGACATGCAAATGATACTTACACCTTGTTAGATTGTGATCCGTACTTGAACGAGACCAAAGAGATGATTCGCGAATTGAACAGAACCAATGAGTTATTTAAATTTGCCAGAGATATGAGAGTGAAGTTTCAAGATGCTGCAGCTCAAGGATTACCAGCTGTTCTTCCTCAAGAGTCTTCCACAATTTCTGGGTCTGCTCCAGTTTTGTCTGTCTCAACTGAGAGAAGTGAATCTCCAGCCAAGATTTATGACCACCAAGTTCAATACGCAGAAGAAAATAGACATTTCAAGAAACCAAACCATGGGAAGGGGAGTAGAGCAGCAATTCTATCTCCTGCCTCTGCATTATCTATGCGTCGGTCTCCTCGTTTAAGGGTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

311

Amino Acids

35.33

Weight (kDa)

8.85

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 158 - 226 1.8e-21 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 438, 848
AccII CGCG 2 cut(s) 138, 618
AciI CCGC 3 cut(s) 136, 144, 474
AclWI GGATC 2 cut(s) 70, 578
AcsI RAATTY 3 cut(s) 488, 530, 647
AcuI CTGAAG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 3 cut(s) 27, 309, 590
AfiI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
AflII CTTAAG 1 cut(s) 311
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 6 cut(s) 191, 206, 287, 296, 689, 704
AluI AGCT 6 cut(s) 191, 206, 287, 296, 689, 704
Alw26I GTCTC 3 cut(s) 593, 763, 912
AlwI GGATC 2 cut(s) 70, 578
AoxI GGCC 4 cut(s) 54, 252, 276, 429
ApeKI GCWGC 4 cut(s) 356, 683, 686, 866
ApoI RAATTY 3 cut(s) 488, 530, 647
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 381
BauI CACGAG 1 cut(s) 80
BbsI GAAGAC 2 cut(s) 245, 714
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 343, 670, 698, 878
BccI CCATC 4 cut(s) 31, 46, 265, 440
BceAI ACGGC 2 cut(s) 22, 120
BciT130I CCWGG 1 cut(s) 125
BciVI GTATCC 1 cut(s) 410
BcoDI GTCTC 3 cut(s) 593, 763, 912
BfaI CTAG 3 cut(s) 230, 288, 297
BfmI CTRYAG 1 cut(s) 684
BfrI CTTAAG 1 cut(s) 311
BfuI GTATCC 1 cut(s) 410
BglI GCCNNNNNGGC 1 cut(s) 104
BisI GCNGC 4 cut(s) 357, 684, 687, 867
BlsI GCNGC 4 cut(s) 358, 685, 688, 868
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 125
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 85, 670
BpiI GAAGAC 2 cut(s) 245, 714
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpmI CTGGAG 3 cut(s) 143, 729, 765
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 675, 699
BsaBI GATNNNNATC 2 cut(s) 74, 582
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 2 cut(s) 593, 912
BsaJI CCNNGG 3 cut(s) 123, 166, 847
BsaWI WCCGGW 1 cut(s) 13
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
Bse1I ACTGG 2 cut(s) 160, 746
Bse8I GATNNNNATC 2 cut(s) 74, 582
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 3 cut(s) 123, 166, 847
BseGI GGATG 4 cut(s) 57, 100, 217, 421
BseJI GATNNNNATC 2 cut(s) 74, 582
BseLI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
BseMII CTCAG 3 cut(s) 198, 383, 756
BseNI ACTGG 2 cut(s) 160, 746
BseRI GAGGAG 2 cut(s) 32, 900
BseXI GCAGC 4 cut(s) 343, 670, 698, 878
Bsh1236I CGCG 2 cut(s) 138, 618
BshFI GGCC 4 cut(s) 56, 254, 278, 431
BsiSI CCGG 1 cut(s) 14
BsiWI CGTACG 1 cut(s) 25
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 5 cut(s) 85, 250, 438, 848, 853
BsmAI GTCTC 3 cut(s) 593, 763, 912
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 4 cut(s) 56, 254, 278, 431
Bso31I GGTCTC 2 cut(s) 593, 912
Bsp119I TTCGAA 1 cut(s) 381
Bsp143I GATC 4 cut(s) 75, 328, 523, 583
Bsp19I CCATGG 1 cut(s) 847
Bsp68I TCGCGA 1 cut(s) 618
BspACI CCGC 3 cut(s) 136, 144, 474
BspANI GGCC 4 cut(s) 56, 254, 278, 431
BspCNI CTCAG 3 cut(s) 199, 382, 757
BspFNI CGCG 2 cut(s) 138, 618
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 2 cut(s) 70, 578
BspT104I TTCGAA 1 cut(s) 381
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 593, 912
BsrI ACTGG 2 cut(s) 160, 746
BssECI CCNNGG 3 cut(s) 123, 166, 847
BssMI GATC 4 cut(s) 75, 328, 523, 583
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 166, 847
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 322, 544
BstACI GRCGYC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 4 cut(s) 207, 339, 369, 765
BstDSI CCRYGG 1 cut(s) 847
BstF5I GGATG 4 cut(s) 57, 100, 217, 421
BstFNI CGCG 2 cut(s) 138, 618
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 78, 331, 526, 586
BstMAI GTCTC 3 cut(s) 593, 763, 912
BstMBI GATC 4 cut(s) 75, 328, 523, 583
BstMWI GCNNNNNNNGC 3 cut(s) 104, 284, 293
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 684
BstUI CGCG 2 cut(s) 138, 618
BstV1I GCAGC 4 cut(s) 343, 670, 698, 878
BstV2I GAAGAC 2 cut(s) 245, 714
BstXI CCANNNNNNTGG 1 cut(s) 262
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 4 cut(s) 56, 254, 278, 431
BtgI CCRYGG 1 cut(s) 847
BtsCI GGATG 4 cut(s) 57, 100, 217, 421
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 240, 327, 540
BtuMI TCGCGA 1 cut(s) 618
Cac8I GCNNGC 1 cut(s) 58
CfoI GCGC 1 cut(s) 140
CseI GACGC 2 cut(s) 96, 890
Csp6I GTAC 3 cut(s) 26, 308, 589
CviAII CATG 3 cut(s) 479, 551, 848
CviQI GTAC 3 cut(s) 26, 308, 589
DdeI CTNAG 4 cut(s) 207, 339, 369, 765
DpnI GATC 4 cut(s) 77, 330, 525, 585
DpnII GATC 4 cut(s) 75, 328, 523, 583
DraI TTTAAA 2 cut(s) 223, 646
EciI GGCGGA 1 cut(s) 489
Eco130I CCWWGG 2 cut(s) 166, 847
Eco31I GGTCTC 2 cut(s) 593, 912
Eco57I CTGAAG 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 2 cut(s) 166, 847
ErhI CCWWGG 2 cut(s) 166, 847
FaeI CATG 3 cut(s) 482, 554, 851
FaiI YATR 8 cut(s) 354, 444, 480, 552, 662, 795, 849, 899
FaqI GGGAC 1 cut(s) 148
FatI CATG 3 cut(s) 478, 550, 847
Fnu4HI GCNGC 4 cut(s) 357, 684, 687, 867
FokI GGATG 4 cut(s) 64, 107, 224, 428
Fsp4HI GCNGC 4 cut(s) 357, 684, 687, 867
FspBI CTAG 3 cut(s) 230, 288, 297
GlaI GCGC 1 cut(s) 139
GluI GCNGC 4 cut(s) 357, 684, 687, 867
GsuI CTGGAG 3 cut(s) 143, 729, 765
HaeIII GGCC 4 cut(s) 56, 254, 278, 431
HapII CCGG 1 cut(s) 14
HgaI GACGC 2 cut(s) 96, 890
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 3 cut(s) 482, 554, 851
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 9 cut(s) 20, 41, 70, 119, 383, 517, 613, 719, 776
HpaII CCGG 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 75, 388
Hpy188III TCNNGA 8 cut(s) 116, 521, 527, 617, 677, 716, 835, 925
Hpy99I CGWCG 2 cut(s) 112, 906
HpyAV CCTTC 4 cut(s) 79, 109, 464, 847
HpyCH4III ACNGT 2 cut(s) 322, 544
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 5 cut(s) 325, 359, 554, 686, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 284, 293
HpyF3I CTNAG 4 cut(s) 207, 339, 369, 765
HpySE526I ACGT 1 cut(s) 465
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 3 cut(s) 482, 554, 851
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 4 cut(s) 75, 328, 523, 583
LmnI GCTCC 1 cut(s) 748
Lsp1109I GCAGC 4 cut(s) 343, 670, 698, 878
LweI GCATC 2 cut(s) 85, 670
MaeI CTAG 3 cut(s) 230, 288, 297
MaeII ACGT 1 cut(s) 465
MalI GATC 4 cut(s) 77, 330, 525, 585
MboI GATC 4 cut(s) 75, 328, 523, 583
MboII GAAGA 8 cut(s) 46, 250, 359, 401, 526, 701, 714, 830
MlyI GAGTC 3 cut(s) 29, 50, 728
MseI TTAA 4 cut(s) 222, 312, 645, 917
MslI CAYNNNNRTG 1 cut(s) 555
MspA1I CMGCKG 1 cut(s) 704
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 2 cut(s) 138, 618
MwoI GCNNNNNNNGC 3 cut(s) 104, 284, 293
NcoI CCATGG 1 cut(s) 847
NdeII GATC 4 cut(s) 75, 328, 523, 583
NlaIII CATG 3 cut(s) 482, 554, 851
NruI TCGCGA 1 cut(s) 618
NspI RCATGY 1 cut(s) 554
NspV TTCGAA 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 910
PfeI GAWTC 6 cut(s) 70, 119, 383, 517, 613, 776
Pfl23II CGTACG 1 cut(s) 25
PflMI CCANNNNNTGG 2 cut(s) 438, 848
PkrI GCNGC 4 cut(s) 358, 685, 688, 868
PleI GAGTC 3 cut(s) 28, 49, 727
PpsI GAGTC 3 cut(s) 28, 49, 727
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspLI CGTACG 1 cut(s) 25
PstI CTGCAG 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 704
RruI TCGCGA 1 cut(s) 618
RsaI GTAC 3 cut(s) 27, 309, 590
RsaNI GTAC 3 cut(s) 26, 308, 589
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 4 cut(s) 222, 312, 645, 917
SatI GCNGC 4 cut(s) 357, 684, 687, 867
Sau3AI GATC 4 cut(s) 75, 328, 523, 583
ScaI AGTACT 1 cut(s) 309
SchI GAGTC 3 cut(s) 29, 50, 728
ScrFI CCNGG 1 cut(s) 125
SetI ASST 9 cut(s) 172, 193, 208, 289, 298, 468, 572, 691, 706
SfaNI GCATC 2 cut(s) 85, 670
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 381
SmiI ATTTAAAT 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 3 cut(s) 311, 690, 714
SmoI CTYRAG 3 cut(s) 311, 690, 714
SsiI CCGC 3 cut(s) 136, 144, 474
SspI AATATT 1 cut(s) 377
SspMI CTAG 3 cut(s) 230, 288, 297
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 166, 847
SwaI ATTTAAAT 1 cut(s) 646
TaaI ACNGT 2 cut(s) 322, 544
TaiI ACGT 1 cut(s) 468
TaqI TCGA 2 cut(s) 381, 547
TaqII GACCGA 1 cut(s) 894
TatI WGTACW 1 cut(s) 307
TfiI GAWTC 6 cut(s) 70, 119, 383, 517, 613, 776
Tru1I TTAA 4 cut(s) 222, 312, 645, 917
Tru9I TTAA 4 cut(s) 222, 312, 645, 917
TscAI CASTG 3 cut(s) 240, 327, 547
TseI GCWGC 4 cut(s) 356, 683, 686, 866
TspDTI ATGAA 1 cut(s) 432
TspGWI ACGGA 5 cut(s) 13, 43, 64, 121, 576
TspRI CASTG 3 cut(s) 240, 327, 547
Van91I CCANNNNNTGG 2 cut(s) 438, 848
Vha464I CTTAAG 1 cut(s) 311
XapI RAATTY 3 cut(s) 488, 530, 647
XceI RCATGY 1 cut(s) 554
XspI CTAG 3 cut(s) 230, 288, 297
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.