FvH4_5g05820

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007257
Physical Location & Seq
Forward (+)
3433271 .. 3435936
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05820.t1

Sequence Viewer

Length: 951 bp
ATGGACTCCAAGCCAGAAGATTCCGTACGGTCGAAGATACAGTCGCAAAGGATGGCCTGCGACAGAGAGATTCGGACTCATCTCCACAAGACCGACGCTGCCACGGCCGCGCTTCACGACTCTCTCGCTTCGATCCGCGCCAAAGTTCGAGAAACCGTCCAATGCCAAGGGAGACTCGGAGAGGCGAAAGCGAAACTGAGAGAAGCCGAGGAGGAATTGTTCAAGGCCCTAGCTGCAAAGACTCGTAAAGAGGCCAAGCGGATGGCAATGATGGATGCAATTTCTGCTAGGAAGGCTAGGATCGACCAACTTAAGAGAGTGGTGCAGGATCAGAGGGCTAAGAGGGATGAGTATGCTGCAATTCTGAAACAGCAATTTTCTGGTACTTTCCTCTTGACAGAATCTGAAGATAGGTCTGTGGAAGACAGTAAAGAAGAAATAGAAGAGGCCATATCATGGTATAATGAGGTCCTTGGTTTCCATGTTGAAGGCGGACAAGGGGTGAGATTCAAGTTCAAGAATGTTAACTCGAAAAATCCAGATGAGGAATTCTCCTTCACTGTTAGGCTCGCAAATGATGTGTACACCTTGTTAGATTGCGATCCACACTTGAATGAGATCAAAGAGTTGTTAGACGAATTGAACAGAACCAATGGATTATTTAAATTTGTCAGAGATATGAGACATAGGTTTCAAGAAGCTGCTTCTCAAGGACTACCAATAGTTCTGCATCAAGAGTATTCAACAATTTCTGGGTCTGCTCCAGCTTTGTCAGTGTCAACAGGAAGTGAATCTATTGCCAAGAGTCATGAGCAACTCCAAGTTCAACATGAAAAACCTGATAGACAAGTCAAGCAATTTAGCCCTGTAAAGGGGAGTAAATCACAAAATCTATCTTCTGGGTCTTTAATGTATCGTCGTCGCTCTCCTTGTTATAAGGTCAGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

317

Amino Acids

35.92

Weight (kDa)

9.0

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spindle_Spc25 PF08234 164 - 232 1.8e-23 Chromosome segregation protein Spc25
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 936
AccB7I CCANNNNNTGG 1 cut(s) 456
AccII CGCG 2 cut(s) 110, 138
AciI CCGC 4 cut(s) 108, 136, 259, 492
AclWI GGATC 4 cut(s) 127, 308, 336, 596
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 2 cut(s) 548, 665
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 3 cut(s) 27, 385, 584
AfiI CCNNNNNNNGG 3 cut(s) 456, 871, 872
AflII CTTAAG 1 cut(s) 311
AgsI TTSAA 9 cut(s) 223, 488, 511, 517, 613, 643, 695, 744, 827
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AluBI AGCT 3 cut(s) 233, 701, 767
AluI AGCT 3 cut(s) 233, 701, 767
Alw26I GTCTC 2 cut(s) 166, 676
AlwI GGATC 4 cut(s) 127, 308, 336, 596
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 5 cut(s) 54, 105, 225, 252, 447
ApeKI GCWGC 4 cut(s) 98, 233, 356, 701
ApoI RAATTY 2 cut(s) 548, 665
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 2 cut(s) 112, 140
AspS9I GGNCC 2 cut(s) 226, 469
AsuHPI GGTGA 1 cut(s) 514
AvaII GGWCC 1 cut(s) 469
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 1 cut(s) 429
BbvI GCAGC 4 cut(s) 85, 220, 343, 688
BccI CCATC 3 cut(s) 46, 256, 265
BceAI ACGGC 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 166, 676
BfaI CTAG 3 cut(s) 230, 288, 297
BfrI CTTAAG 1 cut(s) 311
BisI GCNGC 5 cut(s) 99, 108, 234, 357, 702
BlsI GCNGC 5 cut(s) 100, 109, 235, 358, 703
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 2 cut(s) 226, 469
BmsI GCATC 2 cut(s) 265, 739
BpiI GAAGAC 1 cut(s) 429
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 693
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 102, 166, 207, 472
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 3 cut(s) 456, 871, 872
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 600
BseDI CCNNGG 4 cut(s) 102, 166, 207, 472
BseGI GGATG 4 cut(s) 57, 267, 280, 352
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 3 cut(s) 456, 871, 872
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 224
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 4 cut(s) 85, 220, 343, 688
BsgI GTGCAG 1 cut(s) 344
Bsh1236I CGCG 2 cut(s) 110, 138
Bsh1285I CGRYCG 2 cut(s) 32, 108
BshFI GGCC 5 cut(s) 56, 107, 227, 254, 449
BsiEI CGRYCG 2 cut(s) 32, 108
BsiWI CGTACG 1 cut(s) 25
BslI CCNNNNNNNGG 3 cut(s) 456, 871, 872
BsmAI GTCTC 2 cut(s) 166, 676
BsnI GGCC 5 cut(s) 56, 107, 227, 254, 449
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 5 cut(s) 132, 300, 328, 601, 618
BspACI CCGC 4 cut(s) 108, 136, 259, 492
BspANI GGCC 5 cut(s) 56, 107, 227, 254, 449
BspCNI CTCAG 1 cut(s) 189
BspFNI CGCG 2 cut(s) 110, 138
BspHI TCATGA 1 cut(s) 808
BspPI GGATC 4 cut(s) 127, 308, 336, 596
BspTI CTTAAG 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 582
BssECI CCNNGG 4 cut(s) 102, 166, 207, 472
BssMI GATC 5 cut(s) 132, 300, 328, 601, 618
BssT1I CCWWGG 2 cut(s) 166, 472
Bst4CI ACNGT 5 cut(s) 30, 42, 157, 428, 562
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 582
BstC8I GCNNGC 2 cut(s) 58, 570
BstDEI CTNAG 2 cut(s) 197, 339
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 4 cut(s) 57, 267, 280, 352
BstFNI CGCG 2 cut(s) 110, 138
BstHHI GCGC 2 cut(s) 112, 140
BstKTI GATC 5 cut(s) 135, 303, 331, 604, 621
BstMAI GTCTC 2 cut(s) 166, 676
BstMBI GATC 5 cut(s) 132, 300, 328, 601, 618
BstMCI CGRYCG 2 cut(s) 32, 108
BstMWI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
BstUI CGCG 2 cut(s) 110, 138
BstV1I GCAGC 4 cut(s) 85, 220, 343, 688
BstV2I GAAGAC 1 cut(s) 429
BstXI CCANNNNNNTGG 1 cut(s) 262
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 5 cut(s) 56, 107, 227, 254, 449
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 4 cut(s) 57, 267, 280, 352
BtsIMutI CAGTG 2 cut(s) 558, 780
Cac8I GCNNGC 2 cut(s) 58, 570
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 1 cut(s) 808
CfoI GCGC 2 cut(s) 112, 140
Cfr13I GGNCC 2 cut(s) 226, 469
CseI GACGC 1 cut(s) 104
Csp6I GTAC 3 cut(s) 26, 384, 583
CviAII CATG 4 cut(s) 456, 482, 809, 830
CviQI GTAC 3 cut(s) 26, 384, 583
DdeI CTNAG 2 cut(s) 197, 339
DpnI GATC 5 cut(s) 134, 302, 330, 603, 620
DpnII GATC 5 cut(s) 132, 300, 328, 601, 618
DraI TTTAAA 1 cut(s) 664
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 507
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 166, 472
Eco47I GGWCC 1 cut(s) 469
Eco52I CGGCCG 1 cut(s) 105
Eco57I CTGAAG 1 cut(s) 426
EcoO109I RGGNCCY 2 cut(s) 226, 469
EcoRI GAATTC 1 cut(s) 548
EcoT14I CCWWGG 2 cut(s) 166, 472
ErhI CCWWGG 2 cut(s) 166, 472
FaeI CATG 4 cut(s) 459, 485, 812, 833
FatI CATG 4 cut(s) 455, 481, 808, 829
Fnu4HI GCNGC 5 cut(s) 99, 108, 234, 357, 702
FokI GGATG 4 cut(s) 64, 274, 287, 359
Fsp4HI GCNGC 5 cut(s) 99, 108, 234, 357, 702
FspBI CTAG 3 cut(s) 230, 288, 297
GlaI GCGC 2 cut(s) 111, 139
GluI GCNGC 5 cut(s) 99, 108, 234, 357, 702
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 5 cut(s) 56, 107, 227, 254, 449
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 112, 140
Hin1II CATG 4 cut(s) 459, 485, 812, 833
Hin6I GCGC 2 cut(s) 110, 138
HinP1I GCGC 2 cut(s) 110, 138
HincII GTYRAC 2 cut(s) 526, 780
HindII GTYRAC 2 cut(s) 526, 780
HpaI GTTAAC 1 cut(s) 526
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 4 cut(s) 526, 583, 585, 780
Hpy188I TCNGA 6 cut(s) 75, 179, 333, 366, 406, 674
Hpy188III TCNNGA 8 cut(s) 116, 149, 394, 517, 539, 695, 734, 809
Hpy8I GTNNAC 4 cut(s) 526, 583, 585, 780
Hpy99I CGWCG 3 cut(s) 98, 921, 924
HpyAV CCTTC 3 cut(s) 286, 482, 565
HpyCH4III ACNGT 5 cut(s) 30, 42, 157, 428, 562
HpyCH4V TGCA 5 cut(s) 236, 278, 325, 359, 730
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
HpyF3I CTNAG 2 cut(s) 197, 339
Hsp92II CATG 4 cut(s) 459, 485, 812, 833
HspAI GCGC 2 cut(s) 110, 138
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 5 cut(s) 132, 300, 328, 601, 618
LmnI GCTCC 1 cut(s) 766
Lsp1109I GCAGC 4 cut(s) 85, 220, 343, 688
LweI GCATC 2 cut(s) 265, 739
MaeI CTAG 3 cut(s) 230, 288, 297
MalI GATC 5 cut(s) 134, 302, 330, 603, 620
MboI GATC 5 cut(s) 132, 300, 328, 601, 618
MboII GAAGA 7 cut(s) 29, 46, 419, 434, 446, 455, 888
MluCI AATT 9 cut(s) 215, 279, 360, 374, 548, 638, 665, 747, 857
MlyI GAGTC 5 cut(s) 70, 113, 168, 235, 814
MseI TTAA 4 cut(s) 312, 525, 663, 908
MslI CAYNNNNRTG 1 cut(s) 612
MspCI CTTAAG 1 cut(s) 311
MvnI CGCG 2 cut(s) 110, 138
MwoI GCNNNNNNNGC 5 cut(s) 104, 107, 233, 284, 293
NdeII GATC 5 cut(s) 132, 300, 328, 601, 618
NlaIII CATG 4 cut(s) 459, 485, 812, 833
NmeAIII GCCGAG 1 cut(s) 232
PagI TCATGA 1 cut(s) 808
PfeI GAWTC 5 cut(s) 20, 70, 401, 507, 791
Pfl23II CGTACG 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 5 cut(s) 100, 109, 235, 358, 703
PleI GAGTC 5 cut(s) 70, 113, 168, 235, 813
PpsI GAGTC 5 cut(s) 70, 113, 168, 235, 813
PpuMI RGGWCCY 1 cut(s) 469
PsiI TTATAA 1 cut(s) 936
Psp5II RGGWCCY 1 cut(s) 469
PspLI CGTACG 1 cut(s) 25
PspPI GGNCC 2 cut(s) 226, 469
PspPPI RGGWCCY 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 404
RsaI GTAC 3 cut(s) 27, 385, 584
RsaNI GTAC 3 cut(s) 26, 384, 583
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 4 cut(s) 312, 525, 663, 908
SatI GCNGC 5 cut(s) 99, 108, 234, 357, 702
Sau3AI GATC 5 cut(s) 132, 300, 328, 601, 618
Sau96I GGNCC 2 cut(s) 226, 469
SchI GAGTC 5 cut(s) 70, 113, 168, 235, 814
SetI ASST 9 cut(s) 235, 416, 471, 590, 692, 703, 769, 841, 942
SfaNI GCATC 2 cut(s) 265, 739
SinI GGWCC 1 cut(s) 469
SmiI ATTTAAAT 1 cut(s) 664
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 311, 708
SmoI CTYRAG 2 cut(s) 311, 708
Sse9I AATT 9 cut(s) 215, 279, 360, 374, 548, 638, 665, 747, 857
SsiI CCGC 4 cut(s) 108, 136, 259, 492
SspMI CTAG 3 cut(s) 230, 288, 297
StyI CCWWGG 2 cut(s) 166, 472
SwaI ATTTAAAT 1 cut(s) 664
TaaI ACNGT 5 cut(s) 30, 42, 157, 428, 562
TaqI TCGA 5 cut(s) 32, 131, 148, 303, 530
TaqII GACCGA 1 cut(s) 107
TasI AATT 9 cut(s) 215, 279, 360, 374, 548, 638, 665, 747, 857
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 5 cut(s) 20, 70, 401, 507, 791
Tru1I TTAA 4 cut(s) 312, 525, 663, 908
Tru9I TTAA 4 cut(s) 312, 525, 663, 908
TscAI CASTG 2 cut(s) 565, 780
TseI GCWGC 4 cut(s) 98, 233, 356, 701
TspDTI ATGAA 1 cut(s) 846
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 2 cut(s) 565, 780
Van91I CCANNNNNTGG 1 cut(s) 456
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 2 cut(s) 548, 665
XspI CTAG 3 cut(s) 230, 288, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.