pycom14g14680

Kinetochore protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007257
Physical Location & Seq
Reverse (-)
17392986 .. 17395113
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14680.6

Sequence Viewer

Length: 936 bp
ATGGACGGCAAACCAGAGGAGTCCGTACGGACGAAGATGGAGTCGCTACGGATGGCCTGCGAGAGTGAGATTCCGATCCACCAGCAGGATTTGGATGCCTTCGCGGCGTCGTTTCGGGACTCCCTGGCTTCCGCCCGCGCCACCGCACAAGAAACCCTCCGGTCCCAAGGTAAGTTAGGAGAGGCTAAAGCTAAATTGAGGGAAGCTGAGGATGATTTGTTTAAAGCCCTAGCAGTGAAGACCCGTAAAGAGGCCAAGCGAATGGCATTGATGGAGGCCATTGAAGCTAGGAAAGCTAGGATTGAAGTACTTAAGAGAACAGTGCAAGATCAAAGGGCTAAGAGGGATGAATATGCTGCAATCCTTTCTCAGCAATCTTTTGAGTCTGAAGAAAATTGTATCGGAGACGGAAAGGATGAAATAAAAGAGGCCATCTCGTGGTATAATAGGGTTCTTGGTTTCTACATTGAAGGCGGACATGGTGTAAAATTCACATTCAAGAACATTAGTTTGAAGAATCCAGATCAGGAATTCTGTTTCACCGTTCGCCATGCAAATGATACTTACACCTTGGTAGATTGTGATCGATCGTACTTGAACGAGACCAAAGAGATGATTCATGAATTGAACAGAACCAATGAGTTATTTAAATTTGTCAGAGATATCAGAGTGAAGTTTCAAGAAGCCGCGGCTCAAGGATTACCAACTGTTCTTCCTCAAGATTCTTCCACAATTTCTGGGTCTGCTCCAGTTTTGTCTGTCTCAACTGGGAGAAGTGAATCTCTGGCCGAGATCTATGACCACCAAGTTCAATATGGAGAAGAAAATAGACATTTCAAGAAACCCAACCATGGGAAGGGGAGTAAAGCAGTAATTCAATCTCCTGCCTCTGCATTATCTATGCGTCGGTCTCCTCGTTTAAGGGTTAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spindle_Spc25

Protein Analysis

312

Amino Acids

35.18

Weight (kDa)

8.72

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 438
AccII CGCG 3 cut(s) 104, 138, 689
AciI CCGC 7 cut(s) 104, 132, 136, 144, 474, 687, 689
AclWI GGATC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 3 cut(s) 488, 530, 650
AcuI CTGAAG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 3 cut(s) 27, 309, 593
AfiI CCNNNNNNNGG 6 cut(s) 85, 250, 438, 851, 852, 856
AflII CTTAAG 1 cut(s) 311
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 4 cut(s) 191, 206, 287, 296
AluI AGCT 4 cut(s) 191, 206, 287, 296
Alw26I GTCTC 4 cut(s) 399, 596, 766, 915
AlwI GGATC 1 cut(s) 70
AoxI GGCC 5 cut(s) 54, 252, 276, 429, 786
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 3 cut(s) 488, 530, 650
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 532
AvaII GGWCC 1 cut(s) 162
BauI CACGAG 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 245
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 343
BccI CCATC 4 cut(s) 31, 46, 265, 440
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 4 cut(s) 399, 596, 766, 915
BfaI CTAG 3 cut(s) 230, 288, 297
BfrI CTTAAG 1 cut(s) 311
BglI GCCNNNNNGGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 792
BisI GCNGC 4 cut(s) 105, 357, 687, 690
BlsI GCNGC 4 cut(s) 106, 358, 688, 691
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 162
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 777
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 1 cut(s) 777
BpiI GAAGAC 1 cut(s) 245
BplI GAGNNNNNCTC 2 cut(s) 419, 451
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 2 cut(s) 678, 702
Bsa29I ATCGAT 1 cut(s) 586
BsaBI GATNNNNATC 2 cut(s) 74, 582
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 2 cut(s) 596, 915
BsaJI CCNNGG 5 cut(s) 123, 166, 570, 687, 850
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 250, 438, 851, 852, 856
Bse1I ACTGG 2 cut(s) 749, 772
Bse8I GATNNNNATC 2 cut(s) 74, 582
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 586
BseDI CCNNGG 5 cut(s) 123, 166, 570, 687, 850
BseGI GGATG 5 cut(s) 57, 100, 217, 352, 421
BseJI GATNNNNATC 2 cut(s) 74, 582
BseLI CCNNNNNNNGG 6 cut(s) 85, 250, 438, 851, 852, 856
BseMII CTCAG 2 cut(s) 198, 383
BseNI ACTGG 2 cut(s) 749, 772
BseRI GAGGAG 2 cut(s) 32, 903
BseXI GCAGC 1 cut(s) 343
Bsh1236I CGCG 3 cut(s) 104, 138, 689
Bsh1285I CGRYCG 1 cut(s) 590
BshFI GGCC 5 cut(s) 56, 254, 278, 431, 788
BshVI ATCGAT 1 cut(s) 586
BsiEI CGRYCG 1 cut(s) 590
BsiSI CCGG 1 cut(s) 160
BsiWI CGTACG 1 cut(s) 25
BslFI GGGAC 2 cut(s) 131, 148
BslI CCNNNNNNNGG 6 cut(s) 85, 250, 438, 851, 852, 856
BsmAI GTCTC 4 cut(s) 399, 596, 766, 915
BsmBI CGTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 131, 148
BsnI GGCC 5 cut(s) 56, 254, 278, 431, 788
Bso31I GGTCTC 2 cut(s) 596, 915
Bsp143I GATC 6 cut(s) 75, 328, 523, 583, 587, 792
Bsp19I CCATGG 1 cut(s) 850
BspACI CCGC 7 cut(s) 104, 132, 136, 144, 474, 687, 689
BspANI GGCC 5 cut(s) 56, 254, 278, 431, 788
BspCNI CTCAG 2 cut(s) 199, 382
BspDI ATCGAT 1 cut(s) 586
BspFNI CGCG 3 cut(s) 104, 138, 689
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 1 cut(s) 70
BspTI CTTAAG 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 596, 915
BsrI ACTGG 2 cut(s) 749, 772
BssECI CCNNGG 5 cut(s) 123, 166, 570, 687, 850
BssMI GATC 6 cut(s) 75, 328, 523, 583, 587, 792
BssNI GRCGYC 1 cut(s) 107
BssSI CACGAG 1 cut(s) 436
BssT1I CCWWGG 3 cut(s) 166, 570, 850
Bst2BI CACGAG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 3 cut(s) 322, 544, 709
BstACI GRCGYC 1 cut(s) 107
BstAFI CTTAAG 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 58, 136
BstDEI CTNAG 3 cut(s) 207, 339, 369
BstDSI CCRYGG 2 cut(s) 687, 850
BstF5I GGATG 5 cut(s) 57, 100, 217, 352, 421
BstFNI CGCG 3 cut(s) 104, 138, 689
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 6 cut(s) 78, 331, 526, 586, 590, 795
BstMAI GTCTC 4 cut(s) 399, 596, 766, 915
BstMBI GATC 6 cut(s) 75, 328, 523, 583, 587, 792
BstMCI CGRYCG 1 cut(s) 590
BstMWI GCNNNNNNNGC 3 cut(s) 104, 284, 293
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstUI CGCG 3 cut(s) 104, 138, 689
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 792
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 792
Bsu15I ATCGAT 1 cut(s) 586
BsuRI GGCC 5 cut(s) 56, 254, 278, 431, 788
BsuTUI ATCGAT 1 cut(s) 586
BtgI CCRYGG 2 cut(s) 687, 850
BtsCI GGATG 5 cut(s) 57, 100, 217, 352, 421
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 240, 327
Cac8I GCNNGC 2 cut(s) 58, 136
CciI TCATGA 1 cut(s) 619
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 162
Cfr42I CCGCGG 1 cut(s) 690
ClaI ATCGAT 1 cut(s) 586
CseI GACGC 2 cut(s) 96, 893
Csp6I GTAC 3 cut(s) 26, 308, 592
CviAII CATG 4 cut(s) 479, 551, 620, 851
CviQI GTAC 3 cut(s) 26, 308, 592
DdeI CTNAG 3 cut(s) 207, 339, 369
DpnI GATC 6 cut(s) 77, 330, 525, 585, 589, 794
DpnII GATC 6 cut(s) 75, 328, 523, 583, 587, 792
DraI TTTAAA 2 cut(s) 223, 649
EaeI YGGCCR 1 cut(s) 786
EciI GGCGGA 2 cut(s) 121, 489
Eco130I CCWWGG 3 cut(s) 166, 570, 850
Eco31I GGTCTC 2 cut(s) 596, 915
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 408
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 3 cut(s) 166, 570, 850
ErhI CCWWGG 3 cut(s) 166, 570, 850
Esp3I CGTCTC 1 cut(s) 399
FaeI CATG 4 cut(s) 482, 554, 623, 854
FaiI YATR 9 cut(s) 354, 444, 480, 552, 621, 798, 816, 852, 902
FaqI GGGAC 2 cut(s) 131, 148
FatI CATG 4 cut(s) 478, 550, 619, 850
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 4 cut(s) 105, 357, 687, 690
FokI GGATG 5 cut(s) 64, 107, 224, 359, 428
Fsp4HI GCNGC 4 cut(s) 105, 357, 687, 690
FspBI CTAG 3 cut(s) 230, 288, 297
GlaI GCGC 1 cut(s) 139
GluI GCNGC 4 cut(s) 105, 357, 687, 690
GsuI CTGGAG 1 cut(s) 732
HaeIII GGCC 5 cut(s) 56, 254, 278, 431, 788
HapII CCGG 1 cut(s) 160
HgaI GACGC 2 cut(s) 96, 893
HhaI GCGC 1 cut(s) 140
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 4 cut(s) 482, 554, 623, 854
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 9 cut(s) 20, 41, 70, 119, 383, 517, 616, 722, 779
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 532
Hpy188I TCNGA 5 cut(s) 75, 388, 404, 659, 668
Hpy188III TCNNGA 8 cut(s) 116, 499, 521, 527, 620, 680, 719, 838
Hpy99I CGWCG 2 cut(s) 112, 909
HpyAV CCTTC 3 cut(s) 109, 464, 850
HpyCH4III ACNGT 3 cut(s) 322, 544, 709
HpyCH4V TGCA 4 cut(s) 325, 359, 554, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 284, 293
HpyF3I CTNAG 3 cut(s) 207, 339, 369
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 4 cut(s) 482, 554, 623, 854
HspAI GCGC 1 cut(s) 138
KspI CCGCGG 1 cut(s) 690
Kzo9I GATC 6 cut(s) 75, 328, 523, 583, 587, 792
LmnI GCTCC 1 cut(s) 751
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 85
MaeI CTAG 3 cut(s) 230, 288, 297
MalI GATC 6 cut(s) 77, 330, 525, 585, 589, 794
MboI GATC 6 cut(s) 75, 328, 523, 583, 587, 792
MboII GAAGA 7 cut(s) 46, 250, 401, 526, 704, 717, 833
MflI RGATCY 1 cut(s) 792
MluCI AATT 8 cut(s) 194, 394, 488, 530, 623, 650, 732, 873
MlyI GAGTC 4 cut(s) 29, 50, 113, 392
MseI TTAA 5 cut(s) 222, 312, 648, 920, 927
MslI CAYNNNNRTG 1 cut(s) 555
MspA1I CMGCKG 1 cut(s) 689
MspCI CTTAAG 1 cut(s) 311
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 3 cut(s) 104, 138, 689
MwoI GCNNNNNNNGC 3 cut(s) 104, 284, 293
NcoI CCATGG 1 cut(s) 850
NdeII GATC 6 cut(s) 75, 328, 523, 583, 587, 792
NlaIII CATG 4 cut(s) 482, 554, 623, 854
NlaIV GGNNCC 1 cut(s) 164
NmeAIII GCCGAG 1 cut(s) 814
PagI TCATGA 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PfeI GAWTC 5 cut(s) 70, 517, 616, 722, 779
Pfl23II CGTACG 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 4 cut(s) 106, 358, 688, 691
Ple19I CGATCG 1 cut(s) 590
PleI GAGTC 4 cut(s) 28, 49, 113, 391
PpsI GAGTC 4 cut(s) 28, 49, 113, 391
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspLI CGTACG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 162
PsuI RGATCY 1 cut(s) 792
PvuI CGATCG 1 cut(s) 590
RsaI GTAC 3 cut(s) 27, 309, 593
RsaNI GTAC 3 cut(s) 26, 308, 592
RseI CAYNNNNRTG 1 cut(s) 555
SacII CCGCGG 1 cut(s) 690
SaqAI TTAA 5 cut(s) 222, 312, 648, 920, 927
SatI GCNGC 4 cut(s) 105, 357, 687, 690
Sau3AI GATC 6 cut(s) 75, 328, 523, 583, 587, 792
Sau96I GGNCC 1 cut(s) 162
ScaI AGTACT 1 cut(s) 309
SchI GAGTC 4 cut(s) 29, 50, 113, 392
ScrFI CCNGG 1 cut(s) 125
SetI ASST 6 cut(s) 172, 193, 208, 289, 298, 572
SfaNI GCATC 1 cut(s) 85
Sfr303I CCGCGG 1 cut(s) 690
SgrBI CCGCGG 1 cut(s) 690
SinI GGWCC 1 cut(s) 162
SmiI ATTTAAAT 1 cut(s) 649
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 3 cut(s) 311, 693, 717
SmoI CTYRAG 3 cut(s) 311, 693, 717
Sse9I AATT 8 cut(s) 194, 394, 488, 530, 623, 650, 732, 873
SsiI CCGC 7 cut(s) 104, 132, 136, 144, 474, 687, 689
SspMI CTAG 3 cut(s) 230, 288, 297
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 3 cut(s) 166, 570, 850
SwaI ATTTAAAT 1 cut(s) 649
TaaI ACNGT 3 cut(s) 322, 544, 709
TaqI TCGA 1 cut(s) 586
TaqII GACCGA 1 cut(s) 897
TasI AATT 8 cut(s) 194, 394, 488, 530, 623, 650, 732, 873
TatI WGTACW 1 cut(s) 307
TauI GCSGC 3 cut(s) 107, 689, 692
TfiI GAWTC 5 cut(s) 70, 517, 616, 722, 779
Tru1I TTAA 5 cut(s) 222, 312, 648, 920, 927
Tru9I TTAA 5 cut(s) 222, 312, 648, 920, 927
TscAI CASTG 2 cut(s) 240, 327
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 4 cut(s) 363, 432, 608, 636
TspGWI ACGGA 4 cut(s) 13, 43, 64, 423
TspRI CASTG 2 cut(s) 240, 327
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 3 cut(s) 488, 530, 650
XcmI CCANNNNNNNNNTGG 1 cut(s) 812
XspI CTAG 3 cut(s) 230, 288, 297
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.