AT3G48460

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015101
Physical Location & Seq
Forward (+)
17949416 .. 17951250
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G48460.1

Sequence Viewer

Length: 1146 bp
ATGTCTTCCTCTATCTCTCCCCTCCTCACAACCGCCATCTCCGTCGCAATCCTCCTCTTCTCCACCATCTCCACAGCGGCGACAATTCCAAACATCCACCGTCCATTCAACAAAATCTACGCCTTCGGAGATTCTTTCACCGACACCGGAAACTCACGCTCCGGCGAAGGACCAGCCGGATTCGGACACTTGTCAAGTCCTCCATACGGCATGACTTTCTTCCGACGACCAACAAACCGTTACTCCGACGGTCGTCTAACCATCGACTTCGTGGCAGAGTCAATGAATTTACCGTTTCTGCCACCGTATCTCAGCCTCAAAACCACTAACGCTAACGGCACAGCAACGGATACTCACGGCGTTAATTTCGCTGTCTCTGGATCAACGGTGATTAAACACGCGTTTTTCGTCAAGAACAATCTGTCGCTTGATATGACTCCTCAGTCTATAGAAACAGAGCTTGCTTGGTTTGAGAAGTACTTGGAGACACTTGGAACGAATCAGAAGGTTTCGTTGTTTAAAGATTCTCTCTTTTGGATTGGAGAAATTGGAGTGAATGATTATGCGTATACCCTTGGATCTACTGTGTCAAGTGACACCATTAGAGAACTTAGTATCTCAACTTTTACAAGGTTTTTGGAGACATTGTTGAACAAAGGTGTGAAATATATGTTGGTGCAAGGACATCCAGCAACTGGATGCTTAACGTTAGCAATGTCGTTGGCCGCAGAAGATGATCGGGACAGTCTAGGATGTGTGCAGAGTGCCAATAACCAAAGTTACACTCACAATCTTGCCCTTCAATCCAAACTTAAACAACTTAGGATCAAGTATCCGAGTGCCACAATAGTCTACGCAGATTACTGGAACGCGTACCGTGCGGTGATTAAACACCCTAGCAAGTACGGTATCACCGAGAAGTTCAAGGCGTGTTGTGGAATTGGAGAGCCGTACAACTTCCAAGTGTTCCAAACGTGTGGGACGGATGCAGCTACAGTGTGTAAGGACCCGAATCAATACATCAATTGGGACGGAGTCCACTTGACCGAGGCTATGTATAAGGTTATGGCCGATATGTTCCTTGACGGCACTTTCACTCGTCCTAGATTTAGTGACTTGTTGATCAAGAAACTAAACTATTTATGA

Protein Analysis

381

Amino Acids

42.23

Weight (kDa)

7.61

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 358 1e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 695
AccI GTMKAC 2 cut(s) 569, 852
AccII CGCG 2 cut(s) 401, 872
AciI CCGC 4 cut(s) 33, 77, 726, 881
AclI AACGTT 1 cut(s) 707
AclWI GGATC 3 cut(s) 388, 586, 833
AcoI YGGCCR 2 cut(s) 723, 1068
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 4 cut(s) 479, 875, 905, 953
AfiI CCNNNNNNNGG 2 cut(s) 206, 695
AflIII ACRYGT 3 cut(s) 399, 870, 974
AgsI TTSAA 4 cut(s) 109, 652, 803, 925
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AluBI AGCT 2 cut(s) 460, 992
AluI AGCT 2 cut(s) 460, 992
Alw26I GTCTC 3 cut(s) 379, 479, 635
AlwI GGATC 3 cut(s) 388, 586, 833
AlwNI CAGNNNCTG 1 cut(s) 695
AoxI GGCC 2 cut(s) 723, 1068
ApeKI GCWGC 1 cut(s) 989
ApoI RAATTY 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 170, 1006
AsuHPI GGTGA 4 cut(s) 130, 400, 895, 904
AvaII GGWCC 2 cut(s) 170, 1006
BbvI GCAGC 1 cut(s) 1001
BccI CCATC 3 cut(s) 44, 74, 269
BceAI ACGGC 5 cut(s) 223, 352, 373, 934, 1102
BciVI GTATCC 2 cut(s) 343, 843
BclI TGATCA 1 cut(s) 1122
BcoDI GTCTC 3 cut(s) 379, 479, 635
BfaI CTAG 3 cut(s) 749, 897, 1104
BfmI CTRYAG 2 cut(s) 447, 993
BfuI GTATCC 2 cut(s) 343, 843
BisI GCNGC 3 cut(s) 78, 726, 990
BlsI GCNGC 3 cut(s) 79, 727, 991
BmcAI AGTACT 1 cut(s) 479
Bme18I GGWCC 2 cut(s) 170, 1006
BmgT120I GGNCC 2 cut(s) 170, 1006
BmiI GGNNCC 1 cut(s) 1008
BmsI GCATC 2 cut(s) 689, 976
BoxI GACNNNNGTC 2 cut(s) 190, 252
BsaJI CCNNGG 2 cut(s) 574, 1047
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 6 cut(s) 143, 173, 227, 257, 632, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 695
Bse1I ACTGG 2 cut(s) 700, 869
Bse3DI GCAATG 1 cut(s) 720
BseDI CCNNGG 2 cut(s) 574, 1047
BseGI GGATG 5 cut(s) 93, 685, 704, 758, 991
BseLI CCNNNNNNNGG 2 cut(s) 206, 695
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 325, 455
BseNI ACTGG 2 cut(s) 700, 869
BseRI GAGGAG 3 cut(s) 14, 44, 429
BseXI GCAGC 1 cut(s) 1001
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 401, 872
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 2 cut(s) 725, 1070
BsiEI CGRYCG 1 cut(s) 253
BsiSI CCGG 3 cut(s) 147, 162, 177
BslFI GGGAC 3 cut(s) 755, 994, 1043
BslI CCNNNNNNNGG 2 cut(s) 206, 695
BsmAI GTCTC 3 cut(s) 379, 479, 635
BsmFI GGGAC 3 cut(s) 755, 994, 1043
BsnI GGCC 2 cut(s) 725, 1070
Bsp143I GATC 5 cut(s) 380, 578, 736, 825, 1122
BspACI CCGC 4 cut(s) 33, 77, 726, 881
BspANI GGCC 2 cut(s) 725, 1070
BspCNI CTCAG 2 cut(s) 324, 454
BspFNI CGCG 2 cut(s) 401, 872
BspLI GGNNCC 1 cut(s) 1008
BspPI GGATC 3 cut(s) 388, 586, 833
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 2 cut(s) 700, 869
BssECI CCNNGG 2 cut(s) 574, 1047
BssMI GATC 5 cut(s) 380, 578, 736, 825, 1122
BssNAI GTATAC 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 574
Bst1107I GTATAC 1 cut(s) 570
Bst6I CTCTTC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 462
BstDEI CTNAG 4 cut(s) 311, 441, 611, 821
BstF5I GGATG 5 cut(s) 93, 685, 704, 758, 991
BstFNI CGCG 2 cut(s) 401, 872
BstKTI GATC 5 cut(s) 383, 581, 739, 828, 1125
BstMAI GTCTC 3 cut(s) 379, 479, 635
BstMBI GATC 5 cut(s) 380, 578, 736, 825, 1122
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 878
BstPAI GACNNNNGTC 2 cut(s) 190, 252
BstSFI CTRYAG 2 cut(s) 447, 993
BstUI CGCG 2 cut(s) 401, 872
BstV1I GCAGC 1 cut(s) 1001
BstX2I RGATCY 1 cut(s) 578
BstXI CCANNNNNNTGG 1 cut(s) 977
BstYI RGATCY 1 cut(s) 578
BstZ17I GTATAC 1 cut(s) 570
BsuI GTATCC 2 cut(s) 343, 843
BsuRI GGCC 2 cut(s) 725, 1070
BtsCI GGATG 5 cut(s) 93, 685, 704, 758, 991
BtsIMutI CAGTG 1 cut(s) 1002
Cac8I GCNNGC 1 cut(s) 462
CaiI CAGNNNCTG 1 cut(s) 695
Cfr13I GGNCC 2 cut(s) 170, 1006
Csp6I GTAC 4 cut(s) 478, 874, 904, 952
CviAII CATG 1 cut(s) 211
CviJI RGCY 8 cut(s) 176, 315, 460, 725, 949, 992, 1052, 1070
CviKI_1 RGCY 8 cut(s) 176, 315, 460, 725, 949, 992, 1052, 1070
CviQI GTAC 4 cut(s) 478, 874, 904, 952
DdeI CTNAG 4 cut(s) 311, 441, 611, 821
DpnI GATC 5 cut(s) 382, 580, 738, 827, 1124
DpnII GATC 5 cut(s) 380, 578, 736, 825, 1122
DraI TTTAAA 1 cut(s) 520
DrdI GACNNNNNNGTC 1 cut(s) 442
DseDI GACNNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 2 cut(s) 723, 1068
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 574
Eco47I GGWCC 2 cut(s) 170, 1006
EcoO109I RGGNCCY 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 1 cut(s) 214
FaqI GGGAC 3 cut(s) 755, 994, 1043
FatI CATG 1 cut(s) 210
FbaI TGATCA 1 cut(s) 1122
FblI GTMKAC 2 cut(s) 569, 852
Fnu4HI GCNGC 3 cut(s) 78, 726, 990
FokI GGATG 5 cut(s) 80, 672, 711, 765, 998
Fsp4HI GCNGC 3 cut(s) 78, 726, 990
FspBI CTAG 3 cut(s) 749, 897, 1104
GluI GCNGC 3 cut(s) 78, 726, 990
HaeIII GGCC 2 cut(s) 725, 1070
HapII CCGG 3 cut(s) 147, 162, 177
Hin1II CATG 1 cut(s) 214
HinfI GANTC 8 cut(s) 131, 180, 278, 436, 499, 524, 1012, 1035
HpaII CCGG 3 cut(s) 147, 162, 177
HphI GGTGA 4 cut(s) 130, 400, 895, 904
Hpy166II GTNNAC 3 cut(s) 570, 853, 1039
Hpy188I TCNGA 6 cut(s) 128, 185, 224, 247, 504, 837
Hpy188III TCNNGA 4 cut(s) 378, 412, 740, 1126
Hpy8I GTNNAC 3 cut(s) 570, 853, 1039
Hpy99I CGWCG 3 cut(s) 47, 228, 251
HpyAV CCTTC 4 cut(s) 133, 161, 499, 809
HpyCH4IV ACGT 2 cut(s) 707, 974
HpyCH4V TGCA 3 cut(s) 679, 760, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 878
HpyF3I CTNAG 4 cut(s) 311, 441, 611, 821
HpySE526I ACGT 2 cut(s) 707, 974
Hsp92II CATG 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 5 cut(s) 380, 578, 736, 825, 1122
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 8 cut(s) 160, 175, 186, 190, 363, 681, 702, 850
Lsp1109I GCAGC 1 cut(s) 1001
LweI GCATC 2 cut(s) 689, 976
MaeI CTAG 3 cut(s) 749, 897, 1104
MaeII ACGT 2 cut(s) 707, 974
MaeIII GTNAC 4 cut(s) 239, 593, 779, 1112
MalI GATC 5 cut(s) 382, 580, 738, 827, 1124
MboI GATC 5 cut(s) 380, 578, 736, 825, 1122
MboII GAAGA 3 cut(s) 49, 211, 743
MfeI CAATTG 1 cut(s) 1024
MflI RGATCY 1 cut(s) 578
MluCI AATT 6 cut(s) 84, 286, 364, 546, 939, 1024
MluI ACGCGT 2 cut(s) 399, 870
MlyI GAGTC 3 cut(s) 287, 430, 1044
MmeI TCCRAC 2 cut(s) 247, 270
MnlI CCTC 9 cut(s) 19, 32, 35, 62, 65, 210, 326, 450, 1042
MseI TTAA 6 cut(s) 363, 393, 519, 704, 813, 888
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 3 cut(s) 147, 162, 177
MunI CAATTG 1 cut(s) 1024
MvnI CGCG 2 cut(s) 401, 872
MwoI GCNNNNNNNGC 1 cut(s) 878
NdeII GATC 5 cut(s) 380, 578, 736, 825, 1122
NlaIII CATG 1 cut(s) 214
NlaIV GGNNCC 1 cut(s) 1008
NmuCI GTSAC 2 cut(s) 593, 1112
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 912
PfeI GAWTC 5 cut(s) 131, 180, 499, 524, 1012
PflFI GACNNNGTC 1 cut(s) 1034
PflMI CCANNNNNTGG 1 cut(s) 695
PkrI GCNGC 3 cut(s) 79, 727, 991
PleI GAGTC 3 cut(s) 286, 430, 1043
PpsI GAGTC 3 cut(s) 286, 430, 1043
PpuMI RGGWCCY 1 cut(s) 1006
PshAI GACNNNNGTC 2 cut(s) 190, 252
Psp1406I AACGTT 1 cut(s) 707
Psp5II RGGWCCY 1 cut(s) 1006
PspN4I GGNNCC 1 cut(s) 1008
PspPI GGNCC 2 cut(s) 170, 1006
PspPPI RGGWCCY 1 cut(s) 1006
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 1034
RsaI GTAC 4 cut(s) 479, 875, 905, 953
RsaNI GTAC 4 cut(s) 478, 874, 904, 952
SaqAI TTAA 6 cut(s) 363, 393, 519, 704, 813, 888
SatI GCNGC 3 cut(s) 78, 726, 990
Sau3AI GATC 5 cut(s) 380, 578, 736, 825, 1122
Sau96I GGNCC 2 cut(s) 170, 1006
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 3 cut(s) 287, 430, 1044
SetI ASST 8 cut(s) 462, 510, 635, 661, 710, 977, 994, 1065
SfaNI GCATC 2 cut(s) 689, 976
SfcI CTRYAG 2 cut(s) 447, 993
SinI GGWCC 2 cut(s) 170, 1006
Sse9I AATT 6 cut(s) 84, 286, 364, 546, 939, 1024
SsiI CCGC 4 cut(s) 33, 77, 726, 881
SspMI CTAG 3 cut(s) 749, 897, 1104
StyI CCWWGG 1 cut(s) 574
TaiI ACGT 2 cut(s) 710, 977
TaqI TCGA 1 cut(s) 264
TaqII GACCGA 1 cut(s) 1061
TasI AATT 6 cut(s) 84, 286, 364, 546, 939, 1024
TatI WGTACW 1 cut(s) 477
TauI GCSGC 2 cut(s) 80, 728
TfiI GAWTC 5 cut(s) 131, 180, 499, 524, 1012
Tru1I TTAA 6 cut(s) 363, 393, 519, 704, 813, 888
Tru9I TTAA 6 cut(s) 363, 393, 519, 704, 813, 888
TscAI CASTG 1 cut(s) 1002
TseFI GTSAC 2 cut(s) 593, 1112
TseI GCWGC 1 cut(s) 989
Tsp45I GTSAC 2 cut(s) 593, 1112
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 4 cut(s) 31, 362, 998, 1047
TspRI CASTG 1 cut(s) 1002
Tth111I GACNNNGTC 1 cut(s) 1034
Van91I CCANNNNNTGG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 170, 1006
XapI RAATTY 1 cut(s) 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XmiI GTMKAC 2 cut(s) 569, 852
XspI CTAG 3 cut(s) 749, 897, 1104
ZrmI AGTACT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.