Rmu_co8465791.1_g000001

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8465791.1
OG0015101
Physical Location & Seq
Forward (+)
292 .. 1717
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8465791.1_g000001.1.cds

Sequence Viewer

Length: 1134 bp
atggcgtctatctctttcagtaccatcaccaccttcttcatcttcatcctcacgtcttcctcttgttctgcctctactccttcaactaagacaaccccacatcccttcaagaaaatctatgcctttggtgactccttcactgacacaggcaacacaagatctgtctccggccccagtggctttggccatgttggaaaccccccatatggcattaccttcttccaccgcccgacgaatcgatactccgatgggcggttagtcatagacttcatagctgagtcactgtccttgccttacttgcaaccctacaaaaacatagtgagcaacgcttctagctctgatgcttctcacggggttaactttgctgttgctggctccactgctattaaccatgacttctttgtcaagaacaaccttacacttgatatcactcctcagtctattatgagtcagctgaactggttcaacaagttcttggagactcggggcagcaaagcaggaaattttgatgatgcacttttttgggttggtgaaattggggtcaatgattatgcttacactcttggatcttccataaaaggtgataccatccaaaagcttgggatcagtagtgtcacttgttttctgcaggcattgttaaagaagggtgcaaagtatgtggttgtgcaaggtctgccgatttcggggtgcttgccattatcaatgagcttagctgctgaagacgatagagacagcataggatgtgtgaagagcgtgaacgaccaaacctctgctcacaaccttgtcctcaaggccaagttgcaagatctcaggaaacagtttccgcatgcggtaatcacctatgctgattactggaatgcctattacactgtcatgaagaatccgagccagtacggcttcaaggagagcttcaaggcctgctgtggctccggtgatccctacaacttcaatgtatttgccgtgtgtggcacacgttctgctagtacctgctcgaacccaagccagtacataaattgggatggagttcatctcactgaagccatgtacaaggtgattactaatatgtttgtaagcggcaaagcgactcaccctccattcagctacttgttggacatgaaactgcgtcatggatga

Protein Analysis

377

Amino Acids

41.27

Weight (kDa)

8.64

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 995
AciI CCGC 5 cut(s) 226, 253, 824, 830, 1074
AclWI GGATC 3 cut(s) 574, 611, 929
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 2 cut(s) 738, 1056
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 22, 893, 985, 1007, 1046
AfiI CCNNNNNNNGG 3 cut(s) 206, 252, 683
AflIII ACRYGT 1 cut(s) 971
AgsI TTSAA 6 cut(s) 84, 109, 466, 901, 913, 949
AjiI CACGTC 1 cut(s) 54
AluBI AGCT 8 cut(s) 275, 336, 454, 598, 708, 713, 909, 1101
AluI AGCT 8 cut(s) 275, 336, 454, 598, 708, 713, 909, 1101
Alw26I GTCTC 3 cut(s) 169, 473, 723
AlwI GGATC 3 cut(s) 574, 611, 929
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 4 cut(s) 169, 184, 792, 915
ApeKI GCWGC 2 cut(s) 489, 713
ApoI RAATTY 1 cut(s) 502
Asp700I GAANNNNTTC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 8 cut(s) 19, 140, 542, 593, 829, 944, 1063, 1079
AvaI CYCGRG 1 cut(s) 483
BalI TGGCCA 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 48, 726
BbvI GCAGC 2 cut(s) 501, 700
BccI CCATC 4 cut(s) 32, 242, 596, 1013
BceAI ACGGC 2 cut(s) 910, 944
BcoDI GTCTC 3 cut(s) 169, 473, 723
BfaI CTAG 2 cut(s) 333, 981
BfmI CTRYAG 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 995
BglI GCCNNNNNGGC 2 cut(s) 177, 894
BglII AGATCT 2 cut(s) 158, 805
BisI GCNGC 3 cut(s) 490, 714, 1075
BlpI GCTNAGC 1 cut(s) 709
BlsI GCNGC 3 cut(s) 491, 715, 1076
BmeT110I CYCGRG 1 cut(s) 483
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 3 cut(s) 172, 376, 928
BmrI ACTGGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 331, 502
BmuI ACTGGG 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 48, 726
BplI GAGNNNNNCTC 2 cut(s) 1014, 1046
Bpu1102I GCTNAGC 1 cut(s) 709
BpuEI CTTGAG 1 cut(s) 773
Bsa29I ATCGAT 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 929
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 252, 683
Bse1I ACTGG 5 cut(s) 174, 464, 857, 889, 1003
BseCI ATCGAT 1 cut(s) 238
BseGI GGATG 5 cut(s) 45, 100, 588, 746, 1024
BseLI CCNNNNNNNGG 3 cut(s) 206, 252, 683
BseMII CTCAG 3 cut(s) 267, 449, 823
BseNI ACTGG 5 cut(s) 174, 464, 857, 889, 1003
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 2 cut(s) 501, 700
BshFI GGCC 4 cut(s) 171, 186, 794, 917
BshVI ATCGAT 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 2 cut(s) 168, 930
BslI CCNNNNNNNGG 3 cut(s) 206, 252, 683
BsmAI GTCTC 3 cut(s) 169, 473, 723
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 4 cut(s) 171, 186, 794, 917
BsoBI CYCGRG 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 5 cut(s) 158, 566, 603, 805, 934
Bsp1720I GCTNAGC 1 cut(s) 709
BspACI CCGC 5 cut(s) 226, 253, 824, 830, 1074
BspANI GGCC 4 cut(s) 171, 186, 794, 917
BspCNI CTCAG 3 cut(s) 268, 448, 822
BspDI ATCGAT 1 cut(s) 238
BspHI TCATGA 1 cut(s) 873
BspLI GGNNCC 3 cut(s) 172, 376, 928
BspMAI CTGCAG 1 cut(s) 630
BspMI ACCTGC 1 cut(s) 995
BspPI GGATC 3 cut(s) 574, 611, 929
BspQI GCTCTTC 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 5 cut(s) 174, 464, 857, 889, 1003
BssMI GATC 5 cut(s) 158, 566, 603, 805, 934
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 3 cut(s) 285, 819, 871
Bst6I CTCTTC 1 cut(s) 743
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 673
BstAUI TGTACA 1 cut(s) 1044
BstC8I GCNNGC 5 cut(s) 373, 630, 692, 828, 919
BstDEI CTNAG 5 cut(s) 87, 276, 435, 709, 809
BstF5I GGATG 5 cut(s) 45, 100, 588, 746, 1024
BstKTI GATC 5 cut(s) 161, 569, 606, 808, 937
BstMAI GTCTC 3 cut(s) 169, 473, 723
BstMBI GATC 5 cut(s) 158, 566, 603, 805, 934
BstMWI GCNNNNNNNGC 4 cut(s) 177, 298, 673, 894
BstNSI RCATGY 1 cut(s) 830
BstSFI CTRYAG 1 cut(s) 626
BstV1I GCAGC 2 cut(s) 501, 700
BstV2I GAAGAC 2 cut(s) 48, 726
BstX2I RGATCY 3 cut(s) 158, 566, 805
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 3 cut(s) 158, 566, 805
Bsu15I ATCGAT 1 cut(s) 238
BsuRI GGCC 4 cut(s) 171, 186, 794, 917
BsuTUI ATCGAT 1 cut(s) 238
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 5 cut(s) 45, 100, 588, 746, 1024
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 6 cut(s) 138, 181, 281, 378, 867, 1032
BveI ACCTGC 1 cut(s) 995
Cac8I GCNNGC 5 cut(s) 373, 630, 692, 828, 919
CciI TCATGA 1 cut(s) 873
Cfr13I GGNCC 1 cut(s) 170
ClaI ATCGAT 1 cut(s) 238
CseI GACGC 1 cut(s) 1112
Csp6I GTAC 5 cut(s) 21, 892, 984, 1006, 1045
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 7 cut(s) 188, 392, 827, 874, 1042, 1114, 1127
CviQI GTAC 5 cut(s) 21, 892, 984, 1006, 1045
DdeI CTNAG 5 cut(s) 87, 276, 435, 709, 809
DpnI GATC 5 cut(s) 160, 568, 605, 807, 936
DpnII GATC 5 cut(s) 158, 566, 603, 805, 934
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 743
EarI CTCTTC 1 cut(s) 743
Eco147I AGGCCT 1 cut(s) 917
Eco32I GATATC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 738, 1056
Eco88I CYCGRG 1 cut(s) 483
EcoRV GATATC 1 cut(s) 427
FaeI CATG 7 cut(s) 191, 395, 830, 877, 1045, 1117, 1130
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 7 cut(s) 187, 391, 826, 873, 1041, 1113, 1126
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 490, 714, 1075
FokI GGATG 5 cut(s) 32, 87, 575, 753, 1031
Fsp4HI GCNGC 3 cut(s) 490, 714, 1075
FspBI CTAG 2 cut(s) 333, 981
GluI GCNGC 3 cut(s) 490, 714, 1075
HaeIII GGCC 4 cut(s) 171, 186, 794, 917
HapII CCGG 2 cut(s) 168, 930
HgaI GACGC 1 cut(s) 1112
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 191, 395, 830, 877, 1045, 1117, 1130
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 131, 235, 278, 448, 481, 880, 1084
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 2 cut(s) 168, 930
HphI GGTGA 8 cut(s) 19, 140, 542, 593, 829, 944, 1063, 1079
Hpy166II GTNNAC 2 cut(s) 358, 757
Hpy188I TCNGA 3 cut(s) 247, 340, 885
Hpy188III TCNNGA 4 cut(s) 109, 406, 811, 874
Hpy8I GTNNAC 2 cut(s) 358, 757
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 6 cut(s) 43, 90, 115, 145, 226, 637
HpyCH4III ACNGT 3 cut(s) 285, 819, 871
HpyCH4IV ACGT 2 cut(s) 53, 973
HpyCH4V TGCA 6 cut(s) 301, 515, 628, 650, 667, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 298, 673, 894
HpyF3I CTNAG 5 cut(s) 87, 276, 435, 709, 809
HpySE526I ACGT 2 cut(s) 53, 973
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 191, 395, 830, 877, 1045, 1117, 1130
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 5 cut(s) 158, 566, 603, 805, 934
LguI GCTCTTC 1 cut(s) 743
LmnI GCTCC 2 cut(s) 380, 932
Lsp1109I GCAGC 2 cut(s) 501, 700
LweI GCATC 2 cut(s) 331, 502
MaeI CTAG 2 cut(s) 333, 981
MaeII ACGT 2 cut(s) 53, 973
MaeIII GTNAC 3 cut(s) 128, 279, 613
MalI GATC 5 cut(s) 160, 568, 605, 807, 936
MboI GATC 5 cut(s) 158, 566, 603, 805, 934
MboII GAAGA 8 cut(s) 28, 34, 48, 211, 561, 731, 760, 889
MflI RGATCY 3 cut(s) 158, 566, 805
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 3 cut(s) 502, 534, 1012
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 5 cut(s) 125, 287, 457, 475, 1078
MmeI TCCRAC 2 cut(s) 172, 1089
MnlI CCTC 7 cut(s) 59, 70, 82, 444, 778, 797, 1101
Mox20I TGGCCA 1 cut(s) 186
MroXI GAANNNNTTC 1 cut(s) 461
MscI TGGCCA 1 cut(s) 186
MseI TTAA 3 cut(s) 357, 387, 638
MslI CAYNNNNRTG 1 cut(s) 872
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 2 cut(s) 168, 930
Mva1269I GAATGC 1 cut(s) 862
MwoI GCNNNNNNNGC 4 cut(s) 177, 298, 673, 894
NdeI CATATG 1 cut(s) 205
NdeII GATC 5 cut(s) 158, 566, 603, 805, 934
NlaIII CATG 7 cut(s) 191, 395, 830, 877, 1045, 1117, 1130
NlaIV GGNNCC 3 cut(s) 172, 376, 928
NmuCI GTSAC 3 cut(s) 128, 279, 613
NspI RCATGY 1 cut(s) 830
PaeI GCATGC 1 cut(s) 830
PagI TCATGA 1 cut(s) 873
PceI AGGCCT 1 cut(s) 917
PciSI GCTCTTC 1 cut(s) 743
PctI GAATGC 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 235, 880
PkrI GCNGC 3 cut(s) 491, 715, 1076
PleI GAGTC 5 cut(s) 125, 286, 456, 475, 1078
PpsI GAGTC 5 cut(s) 125, 286, 456, 475, 1078
PspN4I GGNNCC 3 cut(s) 172, 376, 928
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 1 cut(s) 630
PsuI RGATCY 3 cut(s) 158, 566, 805
PvuII CAGCTG 1 cut(s) 454
RsaI GTAC 5 cut(s) 22, 893, 985, 1007, 1046
RsaNI GTAC 5 cut(s) 21, 892, 984, 1006, 1045
RseI CAYNNNNRTG 1 cut(s) 872
SapI GCTCTTC 1 cut(s) 743
SaqAI TTAA 3 cut(s) 357, 387, 638
SatI GCNGC 3 cut(s) 490, 714, 1075
Sau3AI GATC 5 cut(s) 158, 566, 603, 805, 934
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 5 cut(s) 125, 287, 457, 475, 1078
SfaNI GCATC 2 cut(s) 331, 502
SfcI CTRYAG 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
SphI GCATGC 1 cut(s) 830
Sse9I AATT 3 cut(s) 502, 534, 1012
SseBI AGGCCT 1 cut(s) 917
SsiI CCGC 5 cut(s) 226, 253, 824, 830, 1074
SspMI CTAG 2 cut(s) 333, 981
StuI AGGCCT 1 cut(s) 917
TaaI ACNGT 3 cut(s) 285, 819, 871
TaiI ACGT 2 cut(s) 56, 976
TaqI TCGA 2 cut(s) 238, 992
TasI AATT 3 cut(s) 502, 534, 1012
TatI WGTACW 2 cut(s) 1005, 1044
TauI GCSGC 1 cut(s) 1077
TfiI GAWTC 2 cut(s) 235, 880
Tru1I TTAA 3 cut(s) 357, 387, 638
Tru9I TTAA 3 cut(s) 357, 387, 638
TscAI CASTG 6 cut(s) 145, 181, 288, 385, 874, 1039
TseFI GTSAC 3 cut(s) 128, 279, 613
TseI GCWGC 2 cut(s) 489, 713
Tsp45I GTSAC 3 cut(s) 128, 279, 613
TspDTI ATGAA 6 cut(s) 28, 34, 259, 890, 1016, 1130
TspRI CASTG 6 cut(s) 145, 181, 288, 385, 874, 1039
XapI RAATTY 1 cut(s) 502
XceI RCATGY 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 2 cut(s) 333, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.