RchiOBHm_Chr7g0191371

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015101
Physical Location & Seq
Reverse (-)
10141562 .. 10143333
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17100

Sequence Viewer

Length: 1152 bp
ATGCCAGTAAGACCAGCAATGGCGTCTATCTCTTTCAGTACCATCACCACCTTCTTCATCTTCATCCTCACGTCTTCCTCTTGTTCTGCCTCTACTCCTTCAACTAAGACAACCCCACATCCCTTCAAGAAAATCTATGCCTTTGGTGACTCCTTCACTGACACAGGCAACACAAGATCTGTCTCCGGCCCCAGTGGCTTTGGCCATGTTGGAAACCCCCCATATGGCATTACCTTCTTCCACCGCCCGACGAATCGATACTCCGATGGGCGGTTAGTCATAGACTTCATAGCTGAGTCACTGTCCTTGCCTTACTTGCAACCCTACAGAAACATAGTGAGAAATGCTTCTAGCTCTGATGCTTCTCACGGGGTTAACTTTGCTGTTGCTGGCTCCACTGCTATTAACCATGACTTCTTTGTCAAGAACAACCTTACACTTGATATCACTCCTCAGTCTATTATGAGTCAGCTGAACTGGTTCAACAAGTTCTTGGAGACTCAGGGCAGCAAAGCAGGAAATTTTGATGATGCACTTTTTTGGGTTGGTGAAATTGGGGTCAATGATTATGCTTACACTCTTGGATCTTCCATAAAAGGTGATACCATCCAAAAGCTTGGGATCAGTAGTGTCACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGATTTCGGGGTGCTTGCCATTATCAATGAGCTTAGCTGCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGCGTGAACGACCAAACCTCTGCTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGATCTCAGGAAACAGTTTCCGCATGCGGTAATCACCTATGCTGATTACTGGAATGCCTATTACACTGTCATGAAGAATCCGAGCCAGTACGGCTTCAAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGTGATCCCTACAACTTCAATGTATTTGCCGTGTGTGGCACACGTTCTGCTAGTACCTGCTCGAACCCAAGCCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTAATATGTTTGTAAGAGGCAAAGCGACTCACCCTCCATTCAGCTACTTGTTGGACATGAAACTGCGTCATGGATGA

Protein Analysis

383

Amino Acids

42.04

Weight (kDa)

8.81

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 359 7.6e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 1013
AciI CCGC 4 cut(s) 244, 271, 842, 848
AclWI GGATC 3 cut(s) 592, 629, 947
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 2 cut(s) 756, 1074
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 5 cut(s) 40, 911, 1003, 1025, 1064
AfiI CCNNNNNNNGG 3 cut(s) 224, 270, 701
AflIII ACRYGT 1 cut(s) 989
AgsI TTSAA 6 cut(s) 102, 127, 484, 919, 931, 967
AhlI ACTAGT 1 cut(s) 635
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 1 cut(s) 807
AluBI AGCT 8 cut(s) 293, 354, 472, 616, 726, 731, 927, 1119
AluI AGCT 8 cut(s) 293, 354, 472, 616, 726, 731, 927, 1119
Alw26I GTCTC 3 cut(s) 187, 491, 741
AlwI GGATC 3 cut(s) 592, 629, 947
AoxI GGCC 4 cut(s) 187, 202, 810, 933
ApeKI GCWGC 2 cut(s) 507, 731
ApoI RAATTY 1 cut(s) 520
Asp700I GAANNNNTTC 2 cut(s) 346, 479
AspS9I GGNCC 1 cut(s) 188
AsuHPI GGTGA 8 cut(s) 37, 158, 560, 611, 847, 962, 1081, 1097
BalI TGGCCA 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 66, 744
BbvI GCAGC 2 cut(s) 519, 718
BccI CCATC 4 cut(s) 50, 260, 614, 1031
BceAI ACGGC 2 cut(s) 928, 962
BciT130I CCWGG 1 cut(s) 809
BcoDI GTCTC 3 cut(s) 187, 491, 741
BcuI ACTAGT 1 cut(s) 635
BfaI CTAG 3 cut(s) 351, 636, 999
BfmI CTRYAG 2 cut(s) 325, 644
BfuAI ACCTGC 1 cut(s) 1013
BglI GCCNNNNNGGC 2 cut(s) 195, 912
BglII AGATCT 2 cut(s) 176, 823
BisI GCNGC 2 cut(s) 508, 732
BlpI GCTNAGC 1 cut(s) 727
BlsI GCNGC 2 cut(s) 509, 733
Bme1390I CCNGG 1 cut(s) 809
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 188
BmiI GGNNCC 3 cut(s) 190, 394, 946
BmrFI CCNGG 1 cut(s) 809
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 349, 520
BmuI ACTGGG 1 cut(s) 186
BpiI GAAGAC 2 cut(s) 66, 744
BplI GAGNNNNNCTC 2 cut(s) 1032, 1064
BpmI CTGGAG 1 cut(s) 791
Bpu1102I GCTNAGC 1 cut(s) 727
Bsa29I ATCGAT 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 23
BsaWI WCCGGW 1 cut(s) 947
BsaXI ACNNNNNCTCC 2 cut(s) 1093, 1123
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 270, 701
Bse1I ACTGG 6 cut(s) 5, 192, 482, 875, 907, 1021
Bse3DI GCAATG 1 cut(s) 24
BseBI CCWGG 1 cut(s) 809
BseCI ATCGAT 1 cut(s) 256
BseGI GGATG 5 cut(s) 63, 118, 606, 764, 1042
BseLI CCNNNNNNNGG 3 cut(s) 224, 270, 701
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 4 cut(s) 285, 467, 515, 841
BseNI ACTGG 6 cut(s) 5, 192, 482, 875, 907, 1021
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 2 cut(s) 519, 718
BshFI GGCC 4 cut(s) 189, 204, 812, 935
BshVI ATCGAT 1 cut(s) 256
BsiSI CCGG 2 cut(s) 186, 948
BslI CCNNNNNNNGG 3 cut(s) 224, 270, 701
BsmAI GTCTC 3 cut(s) 187, 491, 741
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 4 cut(s) 189, 204, 812, 935
Bsp1407I TGTACA 1 cut(s) 1062
Bsp143I GATC 5 cut(s) 176, 584, 621, 823, 952
Bsp1720I GCTNAGC 1 cut(s) 727
BspACI CCGC 4 cut(s) 244, 271, 842, 848
BspANI GGCC 4 cut(s) 189, 204, 812, 935
BspCNI CTCAG 4 cut(s) 286, 466, 514, 840
BspDI ATCGAT 1 cut(s) 256
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 3 cut(s) 190, 394, 946
BspMAI CTGCAG 1 cut(s) 648
BspMI ACCTGC 1 cut(s) 1013
BspPI GGATC 3 cut(s) 592, 629, 947
BspQI GCTCTTC 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 24
BsrGI TGTACA 1 cut(s) 1062
BsrI ACTGG 6 cut(s) 5, 192, 482, 875, 907, 1021
BssMI GATC 5 cut(s) 176, 584, 621, 823, 952
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 3 cut(s) 303, 837, 889
Bst6I CTCTTC 1 cut(s) 761
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 1062
BstC8I GCNNGC 5 cut(s) 391, 648, 710, 846, 937
BstDEI CTNAG 6 cut(s) 105, 294, 453, 501, 727, 827
BstF5I GGATG 5 cut(s) 63, 118, 606, 764, 1042
BstKTI GATC 5 cut(s) 179, 587, 624, 826, 955
BstMAI GTCTC 3 cut(s) 187, 491, 741
BstMBI GATC 5 cut(s) 176, 584, 621, 823, 952
BstMWI GCNNNNNNNGC 4 cut(s) 195, 316, 691, 912
BstNI CCWGG 1 cut(s) 809
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 1 cut(s) 807
BstSFI CTRYAG 2 cut(s) 325, 644
BstV1I GCAGC 2 cut(s) 519, 718
BstV2I GAAGAC 2 cut(s) 66, 744
BstX2I RGATCY 3 cut(s) 176, 584, 823
BstXI CCANNNNNNTGG 1 cut(s) 617
BstYI RGATCY 3 cut(s) 176, 584, 823
Bsu15I ATCGAT 1 cut(s) 256
BsuRI GGCC 4 cut(s) 189, 204, 812, 935
BsuTUI ATCGAT 1 cut(s) 256
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 5 cut(s) 63, 118, 606, 764, 1042
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 6 cut(s) 156, 199, 299, 396, 885, 1050
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 5 cut(s) 391, 648, 710, 846, 937
CciI TCATGA 1 cut(s) 891
Cfr13I GGNCC 1 cut(s) 188
ClaI ATCGAT 1 cut(s) 256
CseI GACGC 2 cut(s) 12, 1130
Csp6I GTAC 5 cut(s) 39, 910, 1002, 1024, 1063
CspCI CAANNNNNGTGG 2 cut(s) 657, 692
CviAII CATG 7 cut(s) 206, 410, 845, 892, 1060, 1132, 1145
CviQI GTAC 5 cut(s) 39, 910, 1002, 1024, 1063
DdeI CTNAG 6 cut(s) 105, 294, 453, 501, 727, 827
DpnI GATC 5 cut(s) 178, 586, 623, 825, 954
DpnII GATC 5 cut(s) 176, 584, 621, 823, 952
DrdI GACNNNNNNGTC 1 cut(s) 419
DseDI GACNNNNNNGTC 1 cut(s) 419
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 761
EarI CTCTTC 1 cut(s) 761
Eco147I AGGCCT 1 cut(s) 935
Eco32I GATATC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 756, 1074
EcoRII CCWGG 1 cut(s) 807
EcoRV GATATC 1 cut(s) 445
FaeI CATG 7 cut(s) 209, 413, 848, 895, 1063, 1135, 1148
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FatI CATG 7 cut(s) 205, 409, 844, 891, 1059, 1131, 1144
FauNDI CATATG 1 cut(s) 223
Fnu4HI GCNGC 2 cut(s) 508, 732
FokI GGATG 5 cut(s) 50, 105, 593, 771, 1049
Fsp4HI GCNGC 2 cut(s) 508, 732
FspBI CTAG 3 cut(s) 351, 636, 999
GluI GCNGC 2 cut(s) 508, 732
GsuI CTGGAG 1 cut(s) 791
HaeIII GGCC 4 cut(s) 189, 204, 812, 935
HapII CCGG 2 cut(s) 186, 948
HgaI GACGC 2 cut(s) 12, 1130
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 7 cut(s) 209, 413, 848, 895, 1063, 1135, 1148
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 7 cut(s) 149, 253, 296, 466, 499, 898, 1102
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 2 cut(s) 186, 948
HphI GGTGA 8 cut(s) 37, 158, 560, 611, 847, 962, 1081, 1097
Hpy166II GTNNAC 2 cut(s) 376, 775
Hpy188I TCNGA 3 cut(s) 265, 358, 903
Hpy188III TCNNGA 4 cut(s) 127, 424, 829, 892
Hpy8I GTNNAC 2 cut(s) 376, 775
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 6 cut(s) 61, 108, 133, 163, 244, 655
HpyCH4III ACNGT 3 cut(s) 303, 837, 889
HpyCH4IV ACGT 2 cut(s) 71, 991
HpyCH4V TGCA 6 cut(s) 319, 533, 646, 668, 685, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 316, 691, 912
HpyF3I CTNAG 6 cut(s) 105, 294, 453, 501, 727, 827
HpySE526I ACGT 2 cut(s) 71, 991
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 7 cut(s) 209, 413, 848, 895, 1063, 1135, 1148
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 5 cut(s) 176, 584, 621, 823, 952
LguI GCTCTTC 1 cut(s) 761
LmnI GCTCC 2 cut(s) 398, 950
Lsp1109I GCAGC 2 cut(s) 519, 718
LweI GCATC 2 cut(s) 349, 520
MaeI CTAG 3 cut(s) 351, 636, 999
MaeII ACGT 2 cut(s) 71, 991
MaeIII GTNAC 3 cut(s) 146, 297, 631
MalI GATC 5 cut(s) 178, 586, 623, 825, 954
MboI GATC 5 cut(s) 176, 584, 621, 823, 952
MboII GAAGA 8 cut(s) 46, 52, 66, 229, 579, 749, 778, 907
MflI RGATCY 3 cut(s) 176, 584, 823
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 3 cut(s) 520, 552, 1030
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 5 cut(s) 143, 305, 475, 493, 1096
MmeI TCCRAC 2 cut(s) 190, 1107
MnlI CCTC 8 cut(s) 77, 88, 100, 462, 796, 815, 1085, 1119
Mox20I TGGCCA 1 cut(s) 204
MroXI GAANNNNTTC 2 cut(s) 346, 479
MscI TGGCCA 1 cut(s) 204
MseI TTAA 3 cut(s) 375, 405, 656
MslI CAYNNNNRTG 1 cut(s) 890
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 2 cut(s) 186, 948
MspR9I CCNGG 1 cut(s) 809
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 1 cut(s) 809
MwoI GCNNNNNNNGC 4 cut(s) 195, 316, 691, 912
NdeI CATATG 1 cut(s) 223
NdeII GATC 5 cut(s) 176, 584, 621, 823, 952
NlaIII CATG 7 cut(s) 209, 413, 848, 895, 1063, 1135, 1148
NlaIV GGNNCC 3 cut(s) 190, 394, 946
NmuCI GTSAC 3 cut(s) 146, 297, 631
NspI RCATGY 1 cut(s) 848
PaeI GCATGC 1 cut(s) 848
PagI TCATGA 1 cut(s) 891
PceI AGGCCT 1 cut(s) 935
PciSI GCTCTTC 1 cut(s) 761
PctI GAATGC 1 cut(s) 880
PdmI GAANNNNTTC 2 cut(s) 346, 479
PfeI GAWTC 2 cut(s) 253, 898
PkrI GCNGC 2 cut(s) 509, 733
PleI GAGTC 5 cut(s) 143, 304, 474, 493, 1096
PpsI GAGTC 5 cut(s) 143, 304, 474, 493, 1096
Psp6I CCWGG 1 cut(s) 807
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 3 cut(s) 190, 394, 946
PspPI GGNCC 1 cut(s) 188
PstI CTGCAG 1 cut(s) 648
PsuI RGATCY 3 cut(s) 176, 584, 823
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 5 cut(s) 40, 911, 1003, 1025, 1064
RsaNI GTAC 5 cut(s) 39, 910, 1002, 1024, 1063
RseI CAYNNNNRTG 1 cut(s) 890
SapI GCTCTTC 1 cut(s) 761
SaqAI TTAA 3 cut(s) 375, 405, 656
SatI GCNGC 2 cut(s) 508, 732
Sau3AI GATC 5 cut(s) 176, 584, 621, 823, 952
Sau96I GGNCC 1 cut(s) 188
SchI GAGTC 5 cut(s) 143, 305, 475, 493, 1096
ScrFI CCNGG 1 cut(s) 809
SfaNI GCATC 2 cut(s) 349, 520
SfcI CTRYAG 2 cut(s) 325, 644
SmiMI CAYNNNNRTG 1 cut(s) 890
SpeI ACTAGT 1 cut(s) 635
SphI GCATGC 1 cut(s) 848
Sse9I AATT 3 cut(s) 520, 552, 1030
SseBI AGGCCT 1 cut(s) 935
SsiI CCGC 4 cut(s) 244, 271, 842, 848
SspMI CTAG 3 cut(s) 351, 636, 999
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 1 cut(s) 807
TaaI ACNGT 3 cut(s) 303, 837, 889
TaiI ACGT 2 cut(s) 74, 994
TaqI TCGA 2 cut(s) 256, 1010
TasI AATT 3 cut(s) 520, 552, 1030
TatI WGTACW 2 cut(s) 1023, 1062
TfiI GAWTC 2 cut(s) 253, 898
Tru1I TTAA 3 cut(s) 375, 405, 656
Tru9I TTAA 3 cut(s) 375, 405, 656
TscAI CASTG 6 cut(s) 163, 199, 306, 403, 892, 1057
TseFI GTSAC 3 cut(s) 146, 297, 631
TseI GCWGC 2 cut(s) 507, 731
Tsp45I GTSAC 3 cut(s) 146, 297, 631
TspDTI ATGAA 6 cut(s) 46, 52, 277, 908, 1034, 1148
TspRI CASTG 6 cut(s) 163, 199, 306, 403, 892, 1057
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 346, 479
XspI CTAG 3 cut(s) 351, 636, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.