RLG00000004461

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015101
Physical Location & Seq
Forward (+)
59574936 .. 59576367
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004461

Sequence Viewer

Length: 1140 bp
ATGGCGTCCATCTCTTTCAGTACCATCACCACCTTCTTCATCTTCATCCTCATCCTCACCTCTTCCTCTTGTTCTGCCTCTACTCCTTCAACTAAGACAACCCCACATCCCTTCAAGAGAATCTATGCCTTTGGTGACTCCTTCACTGACACAGGCAACACAAGATCTGTCTCCGGCCCCAGTGGCTTTGGCCATGTTGGAAACCCCCCATATGGCATTACCTTCTTCCACCGCCCGACGAATCGATACTCCGATGGGCGGTTAGTCATAGACTTCATAGCTGAGTCACTGTCCTTGCCTTACTTGCAACCCTACAGAAACATACTGAGCAATGCTTCTAGCTCTGATGCTTCTCATGGGGTTAACTTTGCTGTTGCTGGCTCCACTGCTATTAACCATGACTTCTTTGTCAAGAACAACCTTACACTTGATATCACTCCTCAGTCTATTATGAGTCAGCTGAACTGGTTCAACAAGTTCTTGGAGACTCAGGGCAGCAAAGCAGGAAATTTTGATGATGCACTTTTTTGGGTTGGTGAAATTGGGGTCAATGATTATGCTTACACTCTTGGATCTTCCATACCAGGTGATACCATCCAAAAGCTTGGGATCAGTAGTGTCACCAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGATTTCGGGGTGCTTGCCATTAGCAATGAGCTTAGCTGCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGCGTGAACGACCAAACCTCTGCTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGATCTCAGGAAACAGTTTCCTCATGCGGTAATCACCTATGCTGATTACTGGAATGCCTATTACACTGTCATGAAAAATCCAAGCCAGTACGGCTTCCAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGTGATCCCTACAACTTCAATGTATTTGCCGTGTGTGGCACACCTTCTGCTAGTGCCTGCTCGAACCCAAGCCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTGATATGTTTGTAAGCGGCAAAGCGACTCACCCTCCATTCAGCTACTTGTTGGACATGAAGCTGCGTCATGGATGA

Protein Analysis

380

Amino Acids

41.38

Weight (kDa)

6.78

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 356 8.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
AciI CCGC 4 cut(s) 232, 259, 836, 1080
AclWI GGATC 3 cut(s) 580, 617, 935
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 2 cut(s) 744, 1062
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 22, 899, 1013, 1052
AfiI CCNNNNNNNGG 3 cut(s) 212, 258, 689
AgsI TTSAA 5 cut(s) 90, 115, 472, 919, 955
AjnI CCWGG 3 cut(s) 583, 795, 906
AluBI AGCT 9 cut(s) 281, 342, 460, 604, 714, 719, 915, 1107, 1126
AluI AGCT 9 cut(s) 281, 342, 460, 604, 714, 719, 915, 1107, 1126
Alw26I GTCTC 3 cut(s) 175, 479, 729
AlwI GGATC 3 cut(s) 580, 617, 935
AoxI GGCC 4 cut(s) 175, 190, 798, 921
ApeKI GCWGC 3 cut(s) 495, 719, 1126
ApoI RAATTY 1 cut(s) 508
Asp700I GAANNNNTTC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 507, 706, 1113
BccI CCATC 5 cut(s) 17, 32, 248, 602, 1019
BceAI ACGGC 2 cut(s) 916, 950
BciT130I CCWGG 3 cut(s) 585, 797, 908
BcoDI GTCTC 3 cut(s) 175, 479, 729
BfaI CTAG 2 cut(s) 339, 987
BfmI CTRYAG 2 cut(s) 313, 632
BglI GCCNNNNNGGC 2 cut(s) 183, 900
BglII AGATCT 2 cut(s) 164, 811
BisI GCNGC 4 cut(s) 496, 720, 1081, 1127
BlpI GCTNAGC 1 cut(s) 715
BlsI GCNGC 4 cut(s) 497, 721, 1082, 1128
Bme1390I CCNGG 3 cut(s) 585, 797, 908
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 3 cut(s) 178, 382, 934
BmrFI CCNGG 3 cut(s) 585, 797, 908
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 337, 508
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 1020, 1052
BpmI CTGGAG 1 cut(s) 779
Bpu1102I GCTNAGC 1 cut(s) 715
Bsa29I ATCGAT 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 935
BsaXI ACNNNNNCTCC 2 cut(s) 1081, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 258, 689
Bse1I ACTGG 6 cut(s) 180, 470, 624, 863, 895, 1009
Bse3DI GCAATG 2 cut(s) 337, 714
BseBI CCWGG 3 cut(s) 585, 797, 908
BseCI ATCGAT 1 cut(s) 244
BseGI GGATG 6 cut(s) 45, 51, 106, 594, 752, 1030
BseLI CCNNNNNNNGG 3 cut(s) 212, 258, 689
BseMI GCAATG 2 cut(s) 337, 714
BseMII CTCAG 5 cut(s) 273, 317, 455, 503, 829
BseNI ACTGG 6 cut(s) 180, 470, 624, 863, 895, 1009
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 3 cut(s) 507, 706, 1113
BshFI GGCC 4 cut(s) 177, 192, 800, 923
BshVI ATCGAT 1 cut(s) 244
BsiSI CCGG 2 cut(s) 174, 936
BslI CCNNNNNNNGG 3 cut(s) 212, 258, 689
BsmAI GTCTC 3 cut(s) 175, 479, 729
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 4 cut(s) 177, 192, 800, 923
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 5 cut(s) 164, 572, 609, 811, 940
Bsp1720I GCTNAGC 1 cut(s) 715
BspACI CCGC 4 cut(s) 232, 259, 836, 1080
BspANI GGCC 4 cut(s) 177, 192, 800, 923
BspCNI CTCAG 5 cut(s) 274, 318, 454, 502, 828
BspDI ATCGAT 1 cut(s) 244
BspHI TCATGA 1 cut(s) 879
BspLI GGNNCC 3 cut(s) 178, 382, 934
BspMAI CTGCAG 1 cut(s) 636
BspPI GGATC 3 cut(s) 580, 617, 935
BspQI GCTCTTC 1 cut(s) 749
BsrDI GCAATG 2 cut(s) 337, 714
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 6 cut(s) 180, 470, 624, 863, 895, 1009
BssMI GATC 5 cut(s) 164, 572, 609, 811, 940
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 585, 797, 908
Bst4CI ACNGT 3 cut(s) 291, 825, 877
Bst6I CTCTTC 2 cut(s) 67, 749
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 679
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 5 cut(s) 379, 636, 698, 925, 994
BstDEI CTNAG 7 cut(s) 93, 282, 326, 441, 489, 715, 815
BstF5I GGATG 6 cut(s) 45, 51, 106, 594, 752, 1030
BstKTI GATC 5 cut(s) 167, 575, 612, 814, 943
BstMAI GTCTC 3 cut(s) 175, 479, 729
BstMBI GATC 5 cut(s) 164, 572, 609, 811, 940
BstMWI GCNNNNNNNGC 4 cut(s) 183, 304, 679, 900
BstNI CCWGG 3 cut(s) 585, 797, 908
BstSCI CCNGG 3 cut(s) 583, 795, 906
BstSFI CTRYAG 2 cut(s) 313, 632
BstV1I GCAGC 3 cut(s) 507, 706, 1113
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 3 cut(s) 164, 572, 811
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 3 cut(s) 164, 572, 811
Bsu15I ATCGAT 1 cut(s) 244
BsuRI GGCC 4 cut(s) 177, 192, 800, 923
BsuTUI ATCGAT 1 cut(s) 244
BtsCI GGATG 6 cut(s) 45, 51, 106, 594, 752, 1030
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 6 cut(s) 144, 187, 287, 384, 873, 1038
Cac8I GCNNGC 5 cut(s) 379, 636, 698, 925, 994
CciI TCATGA 1 cut(s) 879
Cfr13I GGNCC 1 cut(s) 176
ClaI ATCGAT 1 cut(s) 244
CseI GACGC 1 cut(s) 1118
CsiI ACCWGGT 1 cut(s) 583
Csp6I GTAC 4 cut(s) 21, 898, 1012, 1051
CspCI CAANNNNNGTGG 2 cut(s) 645, 680
CviAII CATG 8 cut(s) 194, 356, 398, 833, 880, 1048, 1120, 1133
CviQI GTAC 4 cut(s) 21, 898, 1012, 1051
DdeI CTNAG 7 cut(s) 93, 282, 326, 441, 489, 715, 815
DpnI GATC 5 cut(s) 166, 574, 611, 813, 942
DpnII GATC 5 cut(s) 164, 572, 609, 811, 940
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 2 cut(s) 67, 749
EarI CTCTTC 2 cut(s) 67, 749
Eco147I AGGCCT 1 cut(s) 923
Eco32I GATATC 1 cut(s) 433
Eco57I CTGAAG 2 cut(s) 744, 1062
EcoRII CCWGG 3 cut(s) 583, 795, 906
EcoRV GATATC 1 cut(s) 433
FaeI CATG 8 cut(s) 197, 359, 401, 836, 883, 1051, 1123, 1136
FatI CATG 8 cut(s) 193, 355, 397, 832, 879, 1047, 1119, 1132
FauNDI CATATG 1 cut(s) 211
Fnu4HI GCNGC 4 cut(s) 496, 720, 1081, 1127
FokI GGATG 6 cut(s) 32, 38, 93, 581, 759, 1037
Fsp4HI GCNGC 4 cut(s) 496, 720, 1081, 1127
FspBI CTAG 2 cut(s) 339, 987
GluI GCNGC 4 cut(s) 496, 720, 1081, 1127
GsuI CTGGAG 1 cut(s) 779
HaeIII GGCC 4 cut(s) 177, 192, 800, 923
HapII CCGG 2 cut(s) 174, 936
HgaI GACGC 1 cut(s) 1118
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 197, 359, 401, 836, 883, 1051, 1123, 1136
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 7 cut(s) 120, 137, 241, 284, 454, 487, 1090
HpaI GTTAAC 1 cut(s) 364
HpaII CCGG 2 cut(s) 174, 936
Hpy166II GTNNAC 2 cut(s) 364, 763
Hpy188I TCNGA 2 cut(s) 253, 346
Hpy188III TCNNGA 4 cut(s) 115, 412, 817, 880
Hpy8I GTNNAC 2 cut(s) 364, 763
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 7 cut(s) 43, 96, 121, 151, 232, 643, 990
HpyCH4III ACNGT 3 cut(s) 291, 825, 877
HpyCH4V TGCA 6 cut(s) 307, 521, 634, 656, 673, 808
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 304, 679, 900
HpyF3I CTNAG 7 cut(s) 93, 282, 326, 441, 489, 715, 815
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 197, 359, 401, 836, 883, 1051, 1123, 1136
KspAI GTTAAC 1 cut(s) 364
Kzo9I GATC 5 cut(s) 164, 572, 609, 811, 940
LguI GCTCTTC 1 cut(s) 749
LmnI GCTCC 2 cut(s) 386, 938
Lsp1109I GCAGC 3 cut(s) 507, 706, 1113
LweI GCATC 2 cut(s) 337, 508
MabI ACCWGGT 1 cut(s) 583
MaeI CTAG 2 cut(s) 339, 987
MaeIII GTNAC 3 cut(s) 134, 285, 619
MalI GATC 5 cut(s) 166, 574, 611, 813, 942
MboI GATC 5 cut(s) 164, 572, 609, 811, 940
MboII GAAGA 7 cut(s) 28, 34, 54, 217, 567, 737, 766
MflI RGATCY 3 cut(s) 164, 572, 811
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 3 cut(s) 508, 540, 1018
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 5 cut(s) 131, 293, 463, 481, 1084
MmeI TCCRAC 2 cut(s) 178, 1095
Mox20I TGGCCA 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 192
MseI TTAA 3 cut(s) 363, 393, 644
MslI CAYNNNNRTG 1 cut(s) 878
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 2 cut(s) 174, 936
MspR9I CCNGG 3 cut(s) 585, 797, 908
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 3 cut(s) 585, 797, 908
MwoI GCNNNNNNNGC 4 cut(s) 183, 304, 679, 900
NdeI CATATG 1 cut(s) 211
NdeII GATC 5 cut(s) 164, 572, 609, 811, 940
NlaIII CATG 8 cut(s) 197, 359, 401, 836, 883, 1051, 1123, 1136
NlaIV GGNNCC 3 cut(s) 178, 382, 934
NmuCI GTSAC 3 cut(s) 134, 285, 619
PagI TCATGA 1 cut(s) 879
PceI AGGCCT 1 cut(s) 923
PciSI GCTCTTC 1 cut(s) 749
PctI GAATGC 1 cut(s) 868
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 120, 241
PfoI TCCNGGA 1 cut(s) 906
PkrI GCNGC 4 cut(s) 497, 721, 1082, 1128
PleI GAGTC 5 cut(s) 131, 292, 462, 481, 1084
PpsI GAGTC 5 cut(s) 131, 292, 462, 481, 1084
Psp6I CCWGG 3 cut(s) 583, 795, 906
PspGI CCWGG 3 cut(s) 583, 795, 906
PspN4I GGNNCC 3 cut(s) 178, 382, 934
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 3 cut(s) 164, 572, 811
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 4 cut(s) 22, 899, 1013, 1052
RsaNI GTAC 4 cut(s) 21, 898, 1012, 1051
RseI CAYNNNNRTG 1 cut(s) 878
SapI GCTCTTC 1 cut(s) 749
SaqAI TTAA 3 cut(s) 363, 393, 644
SatI GCNGC 4 cut(s) 496, 720, 1081, 1127
Sau3AI GATC 5 cut(s) 164, 572, 609, 811, 940
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 5 cut(s) 131, 293, 463, 481, 1084
ScrFI CCNGG 3 cut(s) 585, 797, 908
SexAI ACCWGGT 1 cut(s) 583
SfaNI GCATC 2 cut(s) 337, 508
SfcI CTRYAG 2 cut(s) 313, 632
SmiMI CAYNNNNRTG 1 cut(s) 878
Sse9I AATT 3 cut(s) 508, 540, 1018
SseBI AGGCCT 1 cut(s) 923
SsiI CCGC 4 cut(s) 232, 259, 836, 1080
SspMI CTAG 2 cut(s) 339, 987
StuI AGGCCT 1 cut(s) 923
StyD4I CCNGG 3 cut(s) 583, 795, 906
TaaI ACNGT 3 cut(s) 291, 825, 877
TaqI TCGA 2 cut(s) 244, 998
TasI AATT 3 cut(s) 508, 540, 1018
TatI WGTACW 2 cut(s) 1011, 1050
TauI GCSGC 1 cut(s) 1083
TfiI GAWTC 2 cut(s) 120, 241
Tru1I TTAA 3 cut(s) 363, 393, 644
Tru9I TTAA 3 cut(s) 363, 393, 644
TscAI CASTG 6 cut(s) 151, 187, 294, 391, 880, 1045
TseFI GTSAC 3 cut(s) 134, 285, 619
TseI GCWGC 3 cut(s) 495, 719, 1126
Tsp45I GTSAC 3 cut(s) 134, 285, 619
TspDTI ATGAA 6 cut(s) 28, 34, 265, 896, 1022, 1136
TspRI CASTG 6 cut(s) 151, 187, 294, 391, 880, 1045
XapI RAATTY 1 cut(s) 508
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 2 cut(s) 339, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.