Rh7CG122700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0015101
Physical Location & Seq
Reverse (-)
9477788 .. 9479362
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG122700.1

Sequence Viewer

Length: 690 bp
ATGAGTCAGCTGAACTGGTTCAACAAGTTCTTGGAGACTCAGGGCAGCAAAGCAGGAAATTTTGATGATGCACTTTTTTGGGTTGGTGAAATTGGGGTCAATGATTATGCTTACACTCTTGGATCTTCCATACGAGGTGATACCATCCAAAAGCTTGGGATCAGTAGTGTCACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGATTTCGGGGTGCTTGCCATTAGCAATGAGCTTAGCTGCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGCGTGAACGACCAAACCTCTGCTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGGTCTCAGGAAACAGTTTCCGCATGCGGTAATCACCTATGCTGATTACTGGAATGCCTATTACACTGTCATGAAGAATCCGAGCCAGTACGGCTTCAAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGTGATCCCTACAACTTCAATGTATTTGCCGTGTGTGGCACACGTTCTGCTAGTACCTGCTCGAACCCAAGCCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTGATAGGTTTGTAAGCGGCAAAGCGACTCACCCTCCATTCAGCTACTTGTTGGACATGAAACTGCATCATGGATGA

Protein Analysis

229

Amino Acids

25.26

Weight (kDa)

8.15

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 207 1.7e-15 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 551
AciI CCGC 3 cut(s) 380, 386, 630
AclWI GGATC 3 cut(s) 130, 167, 485
AcsI RAATTY 1 cut(s) 58
AcuI CTGAAG 2 cut(s) 294, 612
AfaI GTAC 4 cut(s) 449, 541, 563, 602
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 527
AgsI TTSAA 4 cut(s) 22, 457, 469, 505
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 1 cut(s) 345
AluBI AGCT 6 cut(s) 10, 154, 264, 269, 465, 657
AluI AGCT 6 cut(s) 10, 154, 264, 269, 465, 657
Alw26I GTCTC 3 cut(s) 29, 279, 368
AlwI GGATC 3 cut(s) 130, 167, 485
AoxI GGCC 2 cut(s) 348, 471
ApeKI GCWGC 2 cut(s) 45, 269
ApoI RAATTY 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 17
AsuHPI GGTGA 6 cut(s) 98, 149, 385, 500, 619, 635
BbsI GAAGAC 1 cut(s) 282
BbvI GCAGC 2 cut(s) 57, 256
BccI CCATC 2 cut(s) 152, 569
BceAI ACGGC 2 cut(s) 466, 500
BciT130I CCWGG 1 cut(s) 347
BcoDI GTCTC 3 cut(s) 29, 279, 368
BcuI ACTAGT 1 cut(s) 173
BfaI CTAG 2 cut(s) 174, 537
BfmI CTRYAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 551
BglI GCCNNNNNGGC 1 cut(s) 450
BisI GCNGC 3 cut(s) 46, 270, 631
BlpI GCTNAGC 1 cut(s) 265
BlsI GCNGC 3 cut(s) 47, 271, 632
Bme1390I CCNGG 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 570, 602
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse1I ACTGG 4 cut(s) 20, 413, 445, 559
Bse3DI GCAATG 1 cut(s) 264
BseBI CCWGG 1 cut(s) 347
BseGI GGATG 3 cut(s) 144, 302, 580
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 53, 379
BseNI ACTGG 4 cut(s) 20, 413, 445, 559
BseXI GCAGC 2 cut(s) 57, 256
BshFI GGCC 2 cut(s) 350, 473
BsiSI CCGG 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 3 cut(s) 29, 279, 368
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 2 cut(s) 350, 473
Bso31I GGTCTC 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 600
Bsp143I GATC 3 cut(s) 122, 159, 490
Bsp1720I GCTNAGC 1 cut(s) 265
BspACI CCGC 3 cut(s) 380, 386, 630
BspANI GGCC 2 cut(s) 350, 473
BspCNI CTCAG 2 cut(s) 52, 378
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 484
BspMAI CTGCAG 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 3 cut(s) 130, 167, 485
BspQI GCTCTTC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 264
BsrGI TGTACA 1 cut(s) 600
BsrI ACTGG 4 cut(s) 20, 413, 445, 559
BssMI GATC 3 cut(s) 122, 159, 490
Bst2UI CCWGG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 375, 427
Bst6I CTCTTC 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 229
BstAUI TGTACA 1 cut(s) 600
BstC8I GCNNGC 4 cut(s) 186, 248, 384, 475
BstDEI CTNAG 3 cut(s) 39, 265, 365
BstF5I GGATG 3 cut(s) 144, 302, 580
BstKTI GATC 3 cut(s) 125, 162, 493
BstMAI GTCTC 3 cut(s) 29, 279, 368
BstMBI GATC 3 cut(s) 122, 159, 490
BstMWI GCNNNNNNNGC 2 cut(s) 229, 450
BstNI CCWGG 1 cut(s) 347
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 345
BstSFI CTRYAG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 57, 256
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 122
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 122
BsuRI GGCC 2 cut(s) 350, 473
BtsCI GGATG 3 cut(s) 144, 302, 580
BtsIMutI CAGTG 2 cut(s) 423, 588
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 4 cut(s) 186, 248, 384, 475
CciI TCATGA 1 cut(s) 429
Csp6I GTAC 4 cut(s) 448, 540, 562, 601
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviAII CATG 5 cut(s) 383, 430, 598, 670, 683
CviQI GTAC 4 cut(s) 448, 540, 562, 601
DdeI CTNAG 3 cut(s) 39, 265, 365
DpnI GATC 3 cut(s) 124, 161, 492
DpnII GATC 3 cut(s) 122, 159, 490
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 294, 612
EcoRII CCWGG 1 cut(s) 345
FaeI CATG 5 cut(s) 386, 433, 601, 673, 686
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FatI CATG 5 cut(s) 382, 429, 597, 669, 682
Fnu4HI GCNGC 3 cut(s) 46, 270, 631
FokI GGATG 3 cut(s) 131, 309, 587
Fsp4HI GCNGC 3 cut(s) 46, 270, 631
FspBI CTAG 2 cut(s) 174, 537
GluI GCNGC 3 cut(s) 46, 270, 631
GsuI CTGGAG 1 cut(s) 329
HaeIII GGCC 2 cut(s) 350, 473
HapII CCGG 1 cut(s) 486
Hin1II CATG 5 cut(s) 386, 433, 601, 673, 686
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 4 cut(s) 4, 37, 436, 640
HpaII CCGG 1 cut(s) 486
HphI GGTGA 6 cut(s) 98, 149, 385, 500, 619, 635
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 1 cut(s) 441
Hpy188III TCNNGA 2 cut(s) 367, 430
Hpy8I GTNNAC 1 cut(s) 313
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 375, 427
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 6 cut(s) 71, 184, 206, 223, 358, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 450
HpyF3I CTNAG 3 cut(s) 39, 265, 365
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 5 cut(s) 386, 433, 601, 673, 686
Kzo9I GATC 3 cut(s) 122, 159, 490
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 2 cut(s) 57, 256
LweI GCATC 1 cut(s) 58
MaeI CTAG 2 cut(s) 174, 537
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 1 cut(s) 169
MalI GATC 3 cut(s) 124, 161, 492
MboI GATC 3 cut(s) 122, 159, 490
MboII GAAGA 4 cut(s) 117, 287, 316, 445
MflI RGATCY 1 cut(s) 122
MluCI AATT 3 cut(s) 58, 90, 568
MlyI GAGTC 3 cut(s) 13, 31, 634
MmeI TCCRAC 1 cut(s) 645
MnlI CCTC 4 cut(s) 128, 334, 353, 657
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 10
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 2 cut(s) 229, 450
NdeII GATC 3 cut(s) 122, 159, 490
NlaIII CATG 5 cut(s) 386, 433, 601, 673, 686
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 169
NspI RCATGY 1 cut(s) 386
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 1 cut(s) 429
PceI AGGCCT 1 cut(s) 473
PciSI GCTCTTC 1 cut(s) 299
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 3 cut(s) 47, 271, 632
PleI GAGTC 3 cut(s) 12, 31, 634
PpsI GAGTC 3 cut(s) 12, 31, 634
Psp6I CCWGG 1 cut(s) 345
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 484
PstI CTGCAG 1 cut(s) 186
PsuI RGATCY 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 10
RsaI GTAC 4 cut(s) 449, 541, 563, 602
RsaNI GTAC 4 cut(s) 448, 540, 562, 601
RseI CAYNNNNRTG 1 cut(s) 428
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 1 cut(s) 194
SatI GCNGC 3 cut(s) 46, 270, 631
Sau3AI GATC 3 cut(s) 122, 159, 490
SchI GAGTC 3 cut(s) 13, 31, 634
ScrFI CCNGG 1 cut(s) 347
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 428
SpeI ACTAGT 1 cut(s) 173
SphI GCATGC 1 cut(s) 386
Sse9I AATT 3 cut(s) 58, 90, 568
SseBI AGGCCT 1 cut(s) 473
SsiI CCGC 3 cut(s) 380, 386, 630
SspMI CTAG 2 cut(s) 174, 537
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 375, 427
TaiI ACGT 1 cut(s) 532
TaqI TCGA 1 cut(s) 548
TasI AATT 3 cut(s) 58, 90, 568
TatI WGTACW 2 cut(s) 561, 600
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 1 cut(s) 194
Tru9I TTAA 1 cut(s) 194
TscAI CASTG 2 cut(s) 430, 595
TseFI GTSAC 1 cut(s) 169
TseI GCWGC 2 cut(s) 45, 269
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 3 cut(s) 446, 572, 686
TspRI CASTG 2 cut(s) 430, 595
XapI RAATTY 1 cut(s) 58
XceI RCATGY 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 2 cut(s) 174, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.