Prupe.5G157800_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015101
Physical Location & Seq
Reverse (-)
13998179 .. 13999903
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G157800.1

Sequence Viewer

Length: 1176 bp
ATGGCTTCTTCTTCAGTCACATATTTCTCTTGGAAAAACACCCTCATTGCCATCACCACCATCTGCATCCTCTCCTCTTCTTCTTGTTCTGCCTCTTCTGCCACAACCAAAGTAAACCCAAGACCAAGACCCTTCAAGAAAATCTATGCCTTTGGTGATTCATTCACAGACACAGGCAACACAAGATCTGCCACAGGCCCTAGTGGTTTTGGCCATGTCTCAAACCCTCCATATGGCACCACTTTCTTTCACCGCCCTACCAACCGCTACTCCGACGGGCGGTTAGTGATCGATTTCATAGCTGAGTCACTGTCCTTGCCTTACTTGAAACCTTACCGTTCCATAATGAGCAATGCTACAGATGCTGCTCATGGGGTTAACTTCGCTGTTGCTGGCTCTACAGCCATAGAGCACCAGTTCTTTGTCAAGAACAACCTGAGCCTTGCAATCACTCCTCAGTCTATCCAAACCCAGCTCCTCTGGTTTGACAAGTTCTTGGAAAGCCAGGGCTGCAAAGTGGCTGGAGCAGCATGCAAAGCAGCAGCTTTTGATGATACACTTTTCTGGGTTGGTGAAATTGGGGTCAATGATTATGCTTATACCATTGGATCTAGTATACCAGGTGATACCATTCAACAGCTTGGGATCAGTAGAGTTACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTTGTTGTGCAAGGCCTGCCACTCTCAGGGTGCTTGCCGTTGGCCATGACCTTAGCTCCTGAAGACGATAGAGACGGTATAGGATGTGTGAAGAGTGCAAACAACCAAACCTATAGCCATAACCTTGTCCTCCAAGCCAAGTTGCAACAACTCAGGACACAGTTTCCCCATGCTGTCATCACCTATGCTGATTACTGGAATGCCTATTTCACAGTCATGAAGAATCCAAACCAGTATGGTTTCAAGGAGAGCTTCAAGGCCTGCTGTGGGACAGGTGACCCCTACAACTTCGAGGTGTTTGCCGTGTGTGGCACACCGTCTGCTAGTGCCTGTCCAAGCCCTTCCCAATACATAAACTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTACTTACTGATATGTTCCTCAATGGCAAGGCCACTCACCCTCCCTTCAGTTACTTGTTGGACATGAAACTGCGTCATGGATGA

Protein Analysis

392

Amino Acids

42.69

Weight (kDa)

8.57

Isoelectric Point (pI)

33.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 236
AccI GTMKAC 1 cut(s) 616
AciI CCGC 3 cut(s) 253, 265, 280
AclWI GGATC 2 cut(s) 616, 653
AcoI YGGCCR 2 cut(s) 211, 741
AcuI CTGAAG 3 cut(s) 780, 1098, 1123
AfaI GTAC 2 cut(s) 1088, 1095
AfiI CCNNNNNNNGG 4 cut(s) 233, 279, 725, 966
AgsI TTSAA 5 cut(s) 136, 328, 635, 943, 955
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 2 cut(s) 504, 619
AluBI AGCT 6 cut(s) 302, 475, 545, 640, 755, 951
AluI AGCT 6 cut(s) 302, 475, 545, 640, 755, 951
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 223, 765
AlwI GGATC 2 cut(s) 616, 653
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 6 cut(s) 196, 211, 712, 741, 957, 1122
ApeKI GCWGC 5 cut(s) 365, 510, 527, 539, 542
AspS9I GGNCC 1 cut(s) 197
AsuHPI GGTGA 8 cut(s) 46, 167, 242, 584, 635, 871, 986, 1121
BalI TGGCCA 2 cut(s) 213, 743
BanI GGYRCC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 5 cut(s) 352, 497, 539, 551, 554
BccI CCATC 3 cut(s) 59, 68, 1055
BceAI ACGGC 2 cut(s) 721, 986
BcgI CGANNNNNNTGC 2 cut(s) 980, 1014
BciT130I CCWGG 2 cut(s) 506, 621
BcoDI GTCTC 2 cut(s) 223, 765
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 4 cut(s) 201, 612, 660, 1023
BfmI CTRYAG 4 cut(s) 357, 399, 668, 811
BglII AGATCT 1 cut(s) 185
BisI GCNGC 5 cut(s) 366, 511, 528, 540, 543
BlsI GCNGC 5 cut(s) 367, 512, 529, 541, 544
Bme1390I CCNGG 2 cut(s) 506, 621
BmgT120I GGNCC 1 cut(s) 197
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 2 cut(s) 506, 621
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 2 cut(s) 75, 352
BmuI ACTGGG 1 cut(s) 1066
BpiI GAAGAC 1 cut(s) 768
BplI GAGNNNNNCTC 2 cut(s) 1056, 1088
BpmI CTGGAG 1 cut(s) 543
Bpu10I CCTNAGC 2 cut(s) 437, 751
Bsa29I ATCGAT 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 505
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 739, 769, 1117, 1147
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 279, 725, 966
Bse1I ACTGG 4 cut(s) 415, 899, 931, 1061
Bse3DI GCAATG 2 cut(s) 45, 358
BseBI CCWGG 2 cut(s) 506, 621
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 3 cut(s) 66, 788, 1066
BseLI CCNNNNNNNGG 4 cut(s) 233, 279, 725, 966
BseMI GCAATG 2 cut(s) 45, 358
BseMII CTCAG 5 cut(s) 294, 428, 470, 738, 865
BseNI ACTGG 4 cut(s) 415, 899, 931, 1061
BseRI GAGGAG 3 cut(s) 64, 444, 467
BseXI GCAGC 5 cut(s) 352, 497, 539, 551, 554
BseYI CCCAGC 1 cut(s) 471
BshFI GGCC 6 cut(s) 198, 213, 714, 743, 959, 1124
BshNI GGYRCC 1 cut(s) 236
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 414
BslFI GGGAC 1 cut(s) 982
BslI CCNNNNNNNGG 4 cut(s) 233, 279, 725, 966
BsmAI GTCTC 2 cut(s) 223, 765
BsmBI CGTCTC 1 cut(s) 765
BsmFI GGGAC 1 cut(s) 982
BsmI GAATGC 1 cut(s) 904
BsnI GGCC 6 cut(s) 198, 213, 714, 743, 959, 1124
Bsp1286I GDGCHC 1 cut(s) 414
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 4 cut(s) 185, 288, 608, 645
BspACI CCGC 3 cut(s) 253, 265, 280
BspANI GGCC 6 cut(s) 198, 213, 714, 743, 959, 1124
BspCNI CTCAG 5 cut(s) 295, 429, 469, 737, 864
BspDI ATCGAT 1 cut(s) 291
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 238
BspMAI CTGCAG 1 cut(s) 672
BspPI GGATC 2 cut(s) 616, 653
BspT107I GGYRCC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 45, 358
BsrGI TGTACA 1 cut(s) 1086
BsrI ACTGG 4 cut(s) 415, 899, 931, 1061
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 4 cut(s) 185, 288, 608, 645
BssNAI GTATAC 1 cut(s) 617
Bst1107I GTATAC 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 506, 621
Bst4CI ACNGT 6 cut(s) 312, 338, 776, 861, 913, 1017
Bst6I CTCTTC 3 cut(s) 82, 100, 785
BstAPI GCANNNNNTGC 1 cut(s) 715
BstAUI TGTACA 1 cut(s) 1086
BstC8I GCNNGC 6 cut(s) 394, 532, 672, 716, 734, 961
BstDEI CTNAG 6 cut(s) 303, 437, 456, 724, 751, 851
BstEII GGTNACC 1 cut(s) 974
BstF5I GGATG 3 cut(s) 66, 788, 1066
BstKTI GATC 4 cut(s) 188, 291, 611, 648
BstMAI GTCTC 2 cut(s) 223, 765
BstMBI GATC 4 cut(s) 185, 288, 608, 645
BstMWI GCNNNNNNNGC 6 cut(s) 98, 362, 510, 527, 536, 715
BstNI CCWGG 2 cut(s) 506, 621
BstNSI RCATGY 1 cut(s) 534
BstPI GGTNACC 1 cut(s) 974
BstSCI CCNGG 2 cut(s) 504, 619
BstSFI CTRYAG 4 cut(s) 357, 399, 668, 811
BstV1I GCAGC 5 cut(s) 352, 497, 539, 551, 554
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 2 cut(s) 185, 608
BstYI RGATCY 2 cut(s) 185, 608
BstZ17I GTATAC 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 6 cut(s) 198, 213, 714, 743, 959, 1124
BsuTUI ATCGAT 1 cut(s) 291
BtsCI GGATG 3 cut(s) 66, 788, 1066
BtsIMutI CAGTG 2 cut(s) 308, 1074
Cac8I GCNNGC 6 cut(s) 394, 532, 672, 716, 734, 961
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 915
Cfr13I GGNCC 1 cut(s) 197
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 1 cut(s) 1154
CsiI ACCWGGT 1 cut(s) 619
Csp6I GTAC 2 cut(s) 1087, 1094
CviAII CATG 9 cut(s) 215, 371, 531, 745, 869, 916, 1084, 1156, 1169
CviQI GTAC 2 cut(s) 1087, 1094
DdeI CTNAG 6 cut(s) 303, 437, 456, 724, 751, 851
DpnI GATC 4 cut(s) 187, 290, 610, 647
DpnII GATC 4 cut(s) 185, 288, 608, 645
EaeI YGGCCR 2 cut(s) 211, 741
Eam1104I CTCTTC 3 cut(s) 82, 100, 785
EarI CTCTTC 3 cut(s) 82, 100, 785
Eco147I AGGCCT 2 cut(s) 714, 959
Eco57I CTGAAG 3 cut(s) 780, 1098, 1123
Eco91I GGTNACC 1 cut(s) 974
EcoO109I RGGNCCY 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 974
EcoRII CCWGG 2 cut(s) 504, 619
Esp3I CGTCTC 1 cut(s) 765
FaeI CATG 9 cut(s) 218, 374, 534, 748, 872, 919, 1087, 1159, 1172
FalI AAGNNNNNCTT 2 cut(s) 935, 967
FaqI GGGAC 1 cut(s) 982
FatI CATG 9 cut(s) 214, 370, 530, 744, 868, 915, 1083, 1155, 1168
FauNDI CATATG 1 cut(s) 232
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 5 cut(s) 366, 511, 528, 540, 543
FokI GGATG 3 cut(s) 53, 795, 1073
Fsp4HI GCNGC 5 cut(s) 366, 511, 528, 540, 543
FspBI CTAG 4 cut(s) 201, 612, 660, 1023
GluI GCNGC 5 cut(s) 366, 511, 528, 540, 543
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 6 cut(s) 198, 213, 714, 743, 959, 1124
HgaI GACGC 1 cut(s) 1154
Hin1II CATG 9 cut(s) 218, 374, 534, 748, 872, 919, 1087, 1159, 1172
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 3 cut(s) 158, 305, 922
HpaI GTTAAC 1 cut(s) 379
HphI GGTGA 8 cut(s) 46, 167, 242, 584, 635, 871, 986, 1121
Hpy166II GTNNAC 3 cut(s) 115, 379, 617
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 5 cut(s) 136, 427, 758, 853, 916
Hpy8I GTNNAC 3 cut(s) 115, 379, 617
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 4 cut(s) 142, 679, 1050, 1147
HpyCH4III ACNGT 6 cut(s) 312, 338, 776, 861, 913, 1017
HpyCH4V TGCA 9 cut(s) 66, 446, 513, 534, 670, 692, 709, 797, 844
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 362, 510, 527, 536, 715
HpyF3I CTNAG 6 cut(s) 303, 437, 456, 724, 751, 851
Hsp92II CATG 9 cut(s) 218, 374, 534, 748, 872, 919, 1087, 1159, 1172
KspAI GTTAAC 1 cut(s) 379
Kzo9I GATC 4 cut(s) 185, 288, 608, 645
LmnI GCTCC 3 cut(s) 480, 524, 760
Lsp1109I GCAGC 5 cut(s) 352, 497, 539, 551, 554
LweI GCATC 2 cut(s) 75, 352
MabI ACCWGGT 1 cut(s) 619
MaeI CTAG 4 cut(s) 201, 612, 660, 1023
MaeIII GTNAC 5 cut(s) 16, 306, 655, 974, 1142
MalI GATC 4 cut(s) 187, 290, 610, 647
MboI GATC 4 cut(s) 185, 288, 608, 645
MboII GAAGA 7 cut(s) 3, 69, 72, 87, 773, 802, 931
MflI RGATCY 2 cut(s) 185, 608
MhlI GDGCHC 1 cut(s) 414
MlsI TGGCCA 2 cut(s) 213, 743
MluCI AATT 1 cut(s) 576
MluNI TGGCCA 2 cut(s) 213, 743
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 2 cut(s) 297, 1131
Mox20I TGGCCA 2 cut(s) 213, 743
MscI TGGCCA 2 cut(s) 213, 743
MseI TTAA 2 cut(s) 378, 680
MslI CAYNNNNRTG 1 cut(s) 914
Msp20I TGGCCA 2 cut(s) 213, 743
MspR9I CCNGG 2 cut(s) 506, 621
Mva1269I GAATGC 1 cut(s) 904
MvaI CCWGG 2 cut(s) 506, 621
MwoI GCNNNNNNNGC 6 cut(s) 98, 362, 510, 527, 536, 715
NdeI CATATG 1 cut(s) 232
NdeII GATC 4 cut(s) 185, 288, 608, 645
NlaIII CATG 9 cut(s) 218, 374, 534, 748, 872, 919, 1087, 1159, 1172
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 3 cut(s) 16, 306, 974
NspI RCATGY 1 cut(s) 534
PaeI GCATGC 1 cut(s) 534
PagI TCATGA 1 cut(s) 915
PceI AGGCCT 2 cut(s) 714, 959
PctI GAATGC 1 cut(s) 904
PfeI GAWTC 2 cut(s) 158, 922
PkrI GCNGC 5 cut(s) 367, 512, 529, 541, 544
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
Psp6I CCWGG 2 cut(s) 504, 619
PspEI GGTNACC 1 cut(s) 974
PspFI CCCAGC 1 cut(s) 471
PspGI CCWGG 2 cut(s) 504, 619
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 197
PstI CTGCAG 1 cut(s) 672
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 2 cut(s) 185, 608
RsaI GTAC 2 cut(s) 1088, 1095
RsaNI GTAC 2 cut(s) 1087, 1094
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 2 cut(s) 378, 680
SatI GCNGC 5 cut(s) 366, 511, 528, 540, 543
Sau3AI GATC 4 cut(s) 185, 288, 608, 645
Sau96I GGNCC 1 cut(s) 197
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 506, 621
SduI GDGCHC 1 cut(s) 414
SexAI ACCWGGT 1 cut(s) 619
SfaNI GCATC 2 cut(s) 75, 352
SfcI CTRYAG 4 cut(s) 357, 399, 668, 811
SmiMI CAYNNNNRTG 1 cut(s) 914
SpeI ACTAGT 1 cut(s) 659
SphI GCATGC 1 cut(s) 534
Sse9I AATT 1 cut(s) 576
SseBI AGGCCT 2 cut(s) 714, 959
SsiI CCGC 3 cut(s) 253, 265, 280
SspMI CTAG 4 cut(s) 201, 612, 660, 1023
StuI AGGCCT 2 cut(s) 714, 959
StyD4I CCNGG 2 cut(s) 504, 619
TaaI ACNGT 6 cut(s) 312, 338, 776, 861, 913, 1017
TaqI TCGA 2 cut(s) 291, 990
TasI AATT 1 cut(s) 576
TatI WGTACW 1 cut(s) 1086
TfiI GAWTC 2 cut(s) 158, 922
Tru1I TTAA 2 cut(s) 378, 680
Tru9I TTAA 2 cut(s) 378, 680
TscAI CASTG 2 cut(s) 315, 1081
TseFI GTSAC 3 cut(s) 16, 306, 974
TseI GCWGC 5 cut(s) 365, 510, 527, 539, 542
Tsp45I GTSAC 3 cut(s) 16, 306, 974
TspDTI ATGAA 5 cut(s) 150, 286, 932, 1058, 1172
TspRI CASTG 2 cut(s) 315, 1081
XceI RCATGY 1 cut(s) 534
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 4 cut(s) 201, 612, 660, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.