FvH4_5g07260

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015101
Physical Location & Seq
Forward (+)
4251958 .. 4253818
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07260.t1

Sequence Viewer

Length: 1128 bp
ATGGCTTCAATATCTTTCATTGCCATCACCACCATCTTCATCTTCATCCTCGCCTCTTCTTCTTGTTATGCCTCTACTCCTTCAACCAAGACAACCTCACATCCCTTCAAGAAAATCTATGCCTTTGGTGACTCCTTCACTGACACAGGCAACACAAGATCTCTCTCTGGCCCTAGTGGCTTTGGCAATGTTGGAAACCCCCCATATGGCATCACCTTCTTCCACCGCCCAACGAATCGATACTCGGACGGGCGGTTAGTCATAGACTTCATAGCTGAATCGCTGTCCTTGCCTTACTTGCAACCCTACAGAAACATAGTGAGCAATGCTTCTAGGTCTGATGCTTCTCATGGGGTCAACTTTGCTGTTGCTGGCTCCACTGCTATTAACCATGACTTCTTTGTCAAGAACAACCTTACACTTGATATCACTCCTCAGTCTATCATGAGTCAGCTGAACTGGTTCAACAAGTTCTTGGAGACTCAGGGCAGCAAAGCAGCAAGTTTTGATGATGCACTTTTTTGGGTCGGTGAGATTGGGGTCAATGACTATGCTTACACTCTTGGATCTACTATACCAGGTGATACCATCCAAAAGCTTGGGATCAGTAGTGTCACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGACTTCGGGGTGCTTGCCATTGGCAATGACCTTAGCTTCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGTGTGAACAACCAAAGCTACAGTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGATCTCAGGAAACAGTTTCCTCATGCAGTAATCACCTATGCTGACTACTGGAATGCCTATTACAGTGTCATGAAGAATCCGAGCCAATACGGCTTCAAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGTGATCCCTACAACTTCAATGTATTCGCCACTTGCGGCGCCCCTTCTGTTAGTGCCTGCTCGAAACCAAGCCAATACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTGATATGTTTGTGAGCGGCAAAGAGACTCACCCTCCATGTAGTTACTTGTTGGACATGCGTCATGGATGA

Protein Analysis

376

Amino Acids

41.04

Weight (kDa)

7.58

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 39 - 354 8.3e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 968
AccBSI CCGCTC 1 cut(s) 1074
AciI CCGC 4 cut(s) 226, 253, 966, 1074
AclWI GGATC 3 cut(s) 574, 611, 929
AcuI CTGAAG 2 cut(s) 738, 1056
AcyI GRCGYC 1 cut(s) 969
AfaI GTAC 1 cut(s) 1046
AfiI CCNNNNNNNGG 2 cut(s) 206, 683
AgsI TTSAA 7 cut(s) 9, 84, 109, 466, 901, 913, 949
AhlI ACTAGT 1 cut(s) 617
AjnI CCWGG 2 cut(s) 577, 789
AluBI AGCT 6 cut(s) 275, 454, 598, 713, 768, 909
AluI AGCT 6 cut(s) 275, 454, 598, 713, 768, 909
Alw26I GTCTC 3 cut(s) 473, 723, 1076
AlwI GGATC 3 cut(s) 574, 611, 929
AoxI GGCC 3 cut(s) 169, 792, 915
ApeKI GCWGC 2 cut(s) 489, 497
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 971
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 9 cut(s) 19, 140, 205, 542, 593, 829, 944, 1063, 1079
BanI GGYRCC 1 cut(s) 968
BbsI GAAGAC 1 cut(s) 726
BbvI GCAGC 2 cut(s) 501, 509
BccI CCATC 4 cut(s) 32, 41, 596, 1013
BceAI ACGGC 1 cut(s) 910
BciT130I CCWGG 2 cut(s) 579, 791
BcoDI GTCTC 3 cut(s) 473, 723, 1076
BcuI ACTAGT 1 cut(s) 617
BfaI CTAG 3 cut(s) 174, 333, 618
BfmI CTRYAG 3 cut(s) 307, 626, 769
BfoI RGCGCY 1 cut(s) 972
BglI GCCNNNNNGGC 2 cut(s) 177, 894
BglII AGATCT 2 cut(s) 158, 805
BisI GCNGC 4 cut(s) 490, 498, 967, 1075
BlsI GCNGC 4 cut(s) 491, 499, 968, 1076
Bme1390I CCNGG 2 cut(s) 579, 791
BmgT120I GGNCC 1 cut(s) 170
BmiI GGNNCC 3 cut(s) 376, 928, 970
BmrFI CCNGG 2 cut(s) 579, 791
BmsI GCATC 3 cut(s) 219, 331, 502
BoxI GACNNNNGTC 1 cut(s) 1116
BpiI GAAGAC 1 cut(s) 726
BplI GAGNNNNNCTC 2 cut(s) 1014, 1046
BpmI CTGGAG 1 cut(s) 773
Bpu10I CCTNAGC 1 cut(s) 709
Bsa29I ATCGAT 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 969
BsaWI WCCGGW 1 cut(s) 929
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 683
Bse1I ACTGG 2 cut(s) 464, 857
Bse3DI GCAATG 4 cut(s) 18, 193, 331, 708
BseBI CCWGG 2 cut(s) 579, 791
BseCI ATCGAT 1 cut(s) 238
BseGI GGATG 5 cut(s) 45, 100, 588, 746, 1024
BseLI CCNNNNNNNGG 2 cut(s) 206, 683
BseMI GCAATG 4 cut(s) 18, 193, 331, 708
BseMII CTCAG 3 cut(s) 449, 497, 823
BseNI ACTGG 2 cut(s) 464, 857
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 2 cut(s) 501, 509
BshFI GGCC 3 cut(s) 171, 794, 917
BshNI GGYRCC 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 238
BsiSI CCGG 1 cut(s) 930
BslI CCNNNNNNNGG 2 cut(s) 206, 683
BsmAI GTCTC 3 cut(s) 473, 723, 1076
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 3 cut(s) 171, 794, 917
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 5 cut(s) 158, 566, 603, 805, 934
BspACI CCGC 4 cut(s) 226, 253, 966, 1074
BspANI GGCC 3 cut(s) 171, 794, 917
BspCNI CTCAG 3 cut(s) 448, 496, 822
BspDI ATCGAT 1 cut(s) 238
BspHI TCATGA 2 cut(s) 444, 873
BspLI GGNNCC 3 cut(s) 376, 928, 970
BspMAI CTGCAG 1 cut(s) 630
BspPI GGATC 3 cut(s) 574, 611, 929
BspT107I GGYRCC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 1074
BsrDI GCAATG 4 cut(s) 18, 193, 331, 708
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 2 cut(s) 464, 857
BssMI GATC 5 cut(s) 158, 566, 603, 805, 934
BssNI GRCGYC 1 cut(s) 969
Bst2UI CCWGG 2 cut(s) 579, 791
Bst4CI ACNGT 3 cut(s) 773, 819, 869
Bst6I CTCTTC 2 cut(s) 61, 743
BstACI GRCGYC 1 cut(s) 969
BstAPI GCANNNNNTGC 1 cut(s) 673
BstAUI TGTACA 1 cut(s) 1044
BstC8I GCNNGC 5 cut(s) 373, 630, 692, 919, 988
BstDEI CTNAG 4 cut(s) 435, 483, 709, 809
BstF5I GGATG 5 cut(s) 45, 100, 588, 746, 1024
BstH2I RGCGCY 1 cut(s) 972
BstHHI GCGC 1 cut(s) 971
BstKTI GATC 5 cut(s) 161, 569, 606, 808, 937
BstMAI GTCTC 3 cut(s) 473, 723, 1076
BstMBI GATC 5 cut(s) 158, 566, 603, 805, 934
BstMWI GCNNNNNNNGC 5 cut(s) 177, 289, 298, 673, 894
BstNI CCWGG 2 cut(s) 579, 791
BstNSI RCATGY 1 cut(s) 1117
BstPAI GACNNNNGTC 1 cut(s) 1116
BstSCI CCNGG 2 cut(s) 577, 789
BstSFI CTRYAG 3 cut(s) 307, 626, 769
BstV1I GCAGC 2 cut(s) 501, 509
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 3 cut(s) 158, 566, 805
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 3 cut(s) 158, 566, 805
Bsu15I ATCGAT 1 cut(s) 238
BsuRI GGCC 3 cut(s) 171, 794, 917
BsuTUI ATCGAT 1 cut(s) 238
BtsCI GGATG 5 cut(s) 45, 100, 588, 746, 1024
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 4 cut(s) 138, 378, 874, 1032
Cac8I GCNNGC 5 cut(s) 373, 630, 692, 919, 988
CciI TCATGA 2 cut(s) 444, 873
CfoI GCGC 1 cut(s) 971
Cfr13I GGNCC 1 cut(s) 170
ClaI ATCGAT 1 cut(s) 238
CseI GACGC 1 cut(s) 1106
CsiI ACCWGGT 1 cut(s) 577
Csp6I GTAC 1 cut(s) 1045
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 9 cut(s) 350, 392, 445, 827, 874, 1042, 1095, 1114, 1121
CviQI GTAC 1 cut(s) 1045
DdeI CTNAG 4 cut(s) 435, 483, 709, 809
DinI GGCGCC 1 cut(s) 970
DpnI GATC 5 cut(s) 160, 568, 605, 807, 936
DpnII GATC 5 cut(s) 158, 566, 603, 805, 934
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 61, 743
EarI CTCTTC 2 cut(s) 61, 743
Eco147I AGGCCT 1 cut(s) 917
Eco32I GATATC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 738, 1056
EcoRII CCWGG 2 cut(s) 577, 789
EcoRV GATATC 1 cut(s) 427
EgeI GGCGCC 1 cut(s) 970
EheI GGCGCC 1 cut(s) 970
FaeI CATG 9 cut(s) 353, 395, 448, 830, 877, 1045, 1098, 1117, 1124
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FatI CATG 9 cut(s) 349, 391, 444, 826, 873, 1041, 1094, 1113, 1120
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 4 cut(s) 490, 498, 967, 1075
FokI GGATG 5 cut(s) 32, 87, 575, 753, 1031
Fsp4HI GCNGC 4 cut(s) 490, 498, 967, 1075
FspBI CTAG 3 cut(s) 174, 333, 618
GlaI GCGC 1 cut(s) 970
GluI GCNGC 4 cut(s) 490, 498, 967, 1075
GsuI CTGGAG 1 cut(s) 773
HaeII RGCGCY 1 cut(s) 972
HaeIII GGCC 3 cut(s) 171, 794, 917
HapII CCGG 1 cut(s) 930
HgaI GACGC 1 cut(s) 1106
HhaI GCGC 1 cut(s) 971
Hin1I GRCGYC 1 cut(s) 969
Hin1II CATG 9 cut(s) 353, 395, 448, 830, 877, 1045, 1098, 1117, 1124
Hin6I GCGC 1 cut(s) 969
HinP1I GCGC 1 cut(s) 969
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 131, 235, 278, 448, 481, 880, 1084
HpaII CCGG 1 cut(s) 930
HphI GGTGA 9 cut(s) 19, 140, 205, 542, 593, 829, 944, 1063, 1079
Hpy166II GTNNAC 2 cut(s) 358, 757
Hpy188I TCNGA 4 cut(s) 247, 340, 718, 885
Hpy188III TCNNGA 5 cut(s) 109, 406, 445, 811, 874
Hpy8I GTNNAC 2 cut(s) 358, 757
HpyAV CCTTC 6 cut(s) 90, 115, 145, 226, 637, 984
HpyCH4III ACNGT 3 cut(s) 773, 819, 869
HpyCH4V TGCA 7 cut(s) 301, 515, 628, 650, 667, 802, 830
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 289, 298, 673, 894
HpyF3I CTNAG 4 cut(s) 435, 483, 709, 809
Hsp92I GRCGYC 1 cut(s) 969
Hsp92II CATG 9 cut(s) 353, 395, 448, 830, 877, 1045, 1098, 1117, 1124
HspAI GCGC 1 cut(s) 969
KasI GGCGCC 1 cut(s) 968
Kzo9I GATC 5 cut(s) 158, 566, 603, 805, 934
LmnI GCTCC 2 cut(s) 380, 932
Lsp1109I GCAGC 2 cut(s) 501, 509
LweI GCATC 3 cut(s) 219, 331, 502
MabI ACCWGGT 1 cut(s) 577
MaeI CTAG 3 cut(s) 174, 333, 618
MaeIII GTNAC 4 cut(s) 128, 613, 773, 1100
MalI GATC 5 cut(s) 160, 568, 605, 807, 936
MbiI CCGCTC 1 cut(s) 1074
MboI GATC 5 cut(s) 158, 566, 603, 805, 934
MboII GAAGA 8 cut(s) 28, 34, 48, 51, 211, 731, 760, 889
MflI RGATCY 3 cut(s) 158, 566, 805
MluCI AATT 1 cut(s) 1012
Mly113I GGCGCC 1 cut(s) 969
MlyI GAGTC 4 cut(s) 125, 457, 475, 1078
MmeI TCCRAC 2 cut(s) 172, 1089
MnlI CCTC 8 cut(s) 59, 64, 82, 106, 444, 797, 834, 1101
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 2 cut(s) 387, 638
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 1 cut(s) 930
MspR9I CCNGG 2 cut(s) 579, 791
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 2 cut(s) 579, 791
MwoI GCNNNNNNNGC 5 cut(s) 177, 289, 298, 673, 894
NarI GGCGCC 1 cut(s) 969
NdeI CATATG 1 cut(s) 205
NdeII GATC 5 cut(s) 158, 566, 603, 805, 934
NlaIII CATG 9 cut(s) 353, 395, 448, 830, 877, 1045, 1098, 1117, 1124
NlaIV GGNNCC 3 cut(s) 376, 928, 970
NmuCI GTSAC 3 cut(s) 128, 613, 773
NspI RCATGY 1 cut(s) 1117
PagI TCATGA 2 cut(s) 444, 873
PceI AGGCCT 1 cut(s) 917
PctI GAATGC 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 461
PfeI GAWTC 3 cut(s) 235, 278, 880
PkrI GCNGC 4 cut(s) 491, 499, 968, 1076
PleI GAGTC 4 cut(s) 125, 456, 475, 1078
PluTI GGCGCC 1 cut(s) 972
PpsI GAGTC 4 cut(s) 125, 456, 475, 1078
PshAI GACNNNNGTC 1 cut(s) 1116
Psp6I CCWGG 2 cut(s) 577, 789
PspGI CCWGG 2 cut(s) 577, 789
PspN4I GGNNCC 3 cut(s) 376, 928, 970
PspPI GGNCC 1 cut(s) 170
PstI CTGCAG 1 cut(s) 630
PsuI RGATCY 3 cut(s) 158, 566, 805
PvuII CAGCTG 1 cut(s) 454
RsaI GTAC 1 cut(s) 1046
RsaNI GTAC 1 cut(s) 1045
SaqAI TTAA 2 cut(s) 387, 638
SatI GCNGC 4 cut(s) 490, 498, 967, 1075
Sau3AI GATC 5 cut(s) 158, 566, 603, 805, 934
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 4 cut(s) 125, 457, 475, 1078
ScrFI CCNGG 2 cut(s) 579, 791
SexAI ACCWGGT 1 cut(s) 577
SfaNI GCATC 3 cut(s) 219, 331, 502
SfcI CTRYAG 3 cut(s) 307, 626, 769
SfoI GGCGCC 1 cut(s) 970
SpeI ACTAGT 1 cut(s) 617
Sse9I AATT 1 cut(s) 1012
SseBI AGGCCT 1 cut(s) 917
SsiI CCGC 4 cut(s) 226, 253, 966, 1074
SspDI GGCGCC 1 cut(s) 968
SspMI CTAG 3 cut(s) 174, 333, 618
StuI AGGCCT 1 cut(s) 917
StyD4I CCNGG 2 cut(s) 577, 789
TaaI ACNGT 3 cut(s) 773, 819, 869
TaqI TCGA 2 cut(s) 238, 992
TasI AATT 1 cut(s) 1012
TatI WGTACW 1 cut(s) 1044
TauI GCSGC 2 cut(s) 969, 1077
TfiI GAWTC 3 cut(s) 235, 278, 880
Tru1I TTAA 2 cut(s) 387, 638
Tru9I TTAA 2 cut(s) 387, 638
TscAI CASTG 4 cut(s) 145, 385, 874, 1039
TseFI GTSAC 3 cut(s) 128, 613, 773
TseI GCWGC 2 cut(s) 489, 497
Tsp45I GTSAC 3 cut(s) 128, 613, 773
TspDTI ATGAA 6 cut(s) 7, 28, 34, 259, 890, 1016
TspRI CASTG 4 cut(s) 145, 385, 874, 1039
XceI RCATGY 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 3 cut(s) 174, 333, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.