Rh7BG119700

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015101
Physical Location & Seq
Reverse (-)
8923279 .. 8925441
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG119700.1

Sequence Viewer

Length: 1047 bp
ATGGTGGTAGTTGAAATCTTTAAAGATGTAGATCAGAGCACCGCCCAAACTCCTACTGAAAGCAACACAAGATCTGTCTCCGGCCCCAGTGGCTTTGGCCATGTTGGAAATCCCCCATATGGCATTACCTTCTTCCACCGCCCGACGAATCGATACTCCGATGGGCGGTTAGTCATAGACTTCATAGCTGAGTCACTGTCCTTGCCTTACTTGCAACCCTACAGAAAAACAGTGAGCAATGCTTCTAGCTCTGATGCTTCTCATGGGGTTAACTTTGCTGTTGCTGGCTCCACTGCTATTAACCATGACTTCTTTGTCAAGAACAACCTTACACTTGATATCACTCCTCAGTCTATCATGAGTCAGCTGAACTGGTTCAACAAGTTCTTGGAGACTCAGGGCAGCAAAGCAGGAAAGTTTGATGATGCACTTTTTTGGGTTGGTGAGATTGGGGTCAATGATTATGCTTACACTCTTGGATCTTCCATACCAGGTGCTACCATCCAAAAGCTTGGGATCAGTAGTGTCACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGATTTCGGGGTGCTTACCATTAGCAATGAGCTTAGCTGCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGCGTGAACGACCGAACCTCTGCTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGATCTCAGGAAACAGTTTCCTCATGCGGTAATCACCTATGCTGATTACTGGAATGCCTATTACACTGTCATGAAGAATCCGAGCCAGTACGGCTTCAAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGCGATCCCTACAACTTCAATGTATTTGCCGTGTGTGGCACACCTTCTGCTAGCGCCTGCTCGAACCCAAGCCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTGATATGTTTGTAAGCGGCAAAGCGACTCACCCTCCATTCAGCTACTTGTTGGACATGAAACTGCGTCATGGATGA

Protein Analysis

348

Amino Acids

38.06

Weight (kDa)

7.1

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 325 2.2e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AciI CCGC 5 cut(s) 42, 139, 166, 743, 987
AclWI GGATC 3 cut(s) 487, 524, 842
AcoI YGGCCR 1 cut(s) 97
AcuI CTGAAG 2 cut(s) 651, 969
AfaI GTAC 3 cut(s) 806, 920, 959
AfiI CCNNNNNNNGG 3 cut(s) 119, 165, 596
AgsI TTSAA 5 cut(s) 14, 379, 814, 826, 862
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 2 cut(s) 490, 702
AluBI AGCT 8 cut(s) 188, 249, 367, 511, 621, 626, 822, 1014
AluI AGCT 8 cut(s) 188, 249, 367, 511, 621, 626, 822, 1014
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 3 cut(s) 82, 386, 636
AlwI GGATC 3 cut(s) 487, 524, 842
AoxI GGCC 4 cut(s) 82, 97, 705, 828
ApeKI GCWGC 2 cut(s) 402, 626
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 1 cut(s) 899
AspS9I GGNCC 1 cut(s) 83
AsuHPI GGTGA 4 cut(s) 455, 742, 976, 992
AsuNHI GCTAGC 1 cut(s) 893
BalI TGGCCA 1 cut(s) 99
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 414, 613
BccI CCATC 3 cut(s) 155, 509, 926
BceAI ACGGC 2 cut(s) 823, 857
BciT130I CCWGG 2 cut(s) 492, 704
BcoDI GTCTC 3 cut(s) 82, 386, 636
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 3 cut(s) 246, 531, 894
BfmI CTRYAG 2 cut(s) 220, 539
BfoI RGCGCY 1 cut(s) 900
BglI GCCNNNNNGGC 2 cut(s) 90, 807
BglII AGATCT 2 cut(s) 71, 718
BisI GCNGC 3 cut(s) 403, 627, 988
BlpI GCTNAGC 1 cut(s) 622
BlsI GCNGC 3 cut(s) 404, 628, 989
Bme1390I CCNGG 2 cut(s) 492, 704
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 3 cut(s) 85, 289, 841
BmrFI CCNGG 2 cut(s) 492, 704
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 244, 415
BmtI GCTAGC 1 cut(s) 897
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 639
BplI GAGNNNNNCTC 2 cut(s) 927, 959
BpmI CTGGAG 1 cut(s) 686
Bpu1102I GCTNAGC 1 cut(s) 622
Bsa29I ATCGAT 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 988, 1018
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 165, 596
Bse1I ACTGG 5 cut(s) 87, 377, 770, 802, 916
Bse3DI GCAATG 2 cut(s) 244, 621
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 2 cut(s) 492, 704
BseCI ATCGAT 1 cut(s) 151
BseGI GGATG 3 cut(s) 501, 659, 937
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 3 cut(s) 119, 165, 596
BseMI GCAATG 2 cut(s) 244, 621
BseMII CTCAG 4 cut(s) 180, 362, 410, 736
BseNI ACTGG 5 cut(s) 87, 377, 770, 802, 916
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 2 cut(s) 414, 613
Bsh1285I CGRYCG 1 cut(s) 676
BshFI GGCC 4 cut(s) 84, 99, 707, 830
BshVI ATCGAT 1 cut(s) 151
BsiEI CGRYCG 1 cut(s) 676
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 2 cut(s) 81, 843
BslI CCNNNNNNNGG 3 cut(s) 119, 165, 596
BsmAI GTCTC 3 cut(s) 82, 386, 636
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 4 cut(s) 84, 99, 707, 830
Bsp1286I GDGCHC 1 cut(s) 41
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 6 cut(s) 31, 71, 479, 516, 718, 847
Bsp1720I GCTNAGC 1 cut(s) 622
BspACI CCGC 5 cut(s) 42, 139, 166, 743, 987
BspANI GGCC 4 cut(s) 84, 99, 707, 830
BspCNI CTCAG 4 cut(s) 181, 361, 409, 735
BspDI ATCGAT 1 cut(s) 151
BspHI TCATGA 2 cut(s) 357, 786
BspLI GGNNCC 3 cut(s) 85, 289, 841
BspMAI CTGCAG 1 cut(s) 543
BspOI GCTAGC 1 cut(s) 897
BspPI GGATC 3 cut(s) 487, 524, 842
BspQI GCTCTTC 1 cut(s) 656
BsrDI GCAATG 2 cut(s) 244, 621
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 5 cut(s) 87, 377, 770, 802, 916
BssMI GATC 6 cut(s) 31, 71, 479, 516, 718, 847
Bst2UI CCWGG 2 cut(s) 492, 704
Bst4CI ACNGT 4 cut(s) 198, 232, 732, 784
Bst6I CTCTTC 1 cut(s) 656
BstAPI GCANNNNNTGC 1 cut(s) 586
BstAUI TGTACA 1 cut(s) 957
BstC8I GCNNGC 5 cut(s) 286, 543, 832, 895, 901
BstDEI CTNAG 5 cut(s) 189, 348, 396, 622, 722
BstF5I GGATG 3 cut(s) 501, 659, 937
BstH2I RGCGCY 1 cut(s) 900
BstHHI GCGC 1 cut(s) 899
BstKTI GATC 6 cut(s) 34, 74, 482, 519, 721, 850
BstMAI GTCTC 3 cut(s) 82, 386, 636
BstMBI GATC 6 cut(s) 31, 71, 479, 516, 718, 847
BstMCI CGRYCG 1 cut(s) 676
BstMWI GCNNNNNNNGC 4 cut(s) 90, 211, 586, 807
BstNI CCWGG 2 cut(s) 492, 704
BstSCI CCNGG 2 cut(s) 490, 702
BstSFI CTRYAG 2 cut(s) 220, 539
BstV1I GCAGC 2 cut(s) 414, 613
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 3 cut(s) 71, 479, 718
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 3 cut(s) 71, 479, 718
Bsu15I ATCGAT 1 cut(s) 151
BsuRI GGCC 4 cut(s) 84, 99, 707, 830
BsuTUI ATCGAT 1 cut(s) 151
BtsCI GGATG 3 cut(s) 501, 659, 937
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 6 cut(s) 94, 194, 237, 291, 780, 945
Cac8I GCNNGC 5 cut(s) 286, 543, 832, 895, 901
CciI TCATGA 2 cut(s) 357, 786
CfoI GCGC 1 cut(s) 899
Cfr13I GGNCC 1 cut(s) 83
ClaI ATCGAT 1 cut(s) 151
CseI GACGC 1 cut(s) 1025
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 3 cut(s) 805, 919, 958
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 9 cut(s) 101, 263, 305, 358, 740, 787, 955, 1027, 1040
CviQI GTAC 3 cut(s) 805, 919, 958
DdeI CTNAG 5 cut(s) 189, 348, 396, 622, 722
DpnI GATC 6 cut(s) 33, 73, 481, 518, 720, 849
DpnII GATC 6 cut(s) 31, 71, 479, 516, 718, 847
DraI TTTAAA 1 cut(s) 22
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Eco147I AGGCCT 1 cut(s) 830
Eco32I GATATC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 651, 969
EcoRII CCWGG 2 cut(s) 490, 702
EcoRV GATATC 1 cut(s) 340
FaeI CATG 9 cut(s) 104, 266, 308, 361, 743, 790, 958, 1030, 1043
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FatI CATG 9 cut(s) 100, 262, 304, 357, 739, 786, 954, 1026, 1039
FauNDI CATATG 1 cut(s) 118
Fnu4HI GCNGC 3 cut(s) 403, 627, 988
FokI GGATG 3 cut(s) 488, 666, 944
Fsp4HI GCNGC 3 cut(s) 403, 627, 988
FspBI CTAG 3 cut(s) 246, 531, 894
GlaI GCGC 1 cut(s) 898
GluI GCNGC 3 cut(s) 403, 627, 988
GsuI CTGGAG 1 cut(s) 686
HaeII RGCGCY 1 cut(s) 900
HaeIII GGCC 4 cut(s) 84, 99, 707, 830
HapII CCGG 2 cut(s) 81, 843
HgaI GACGC 1 cut(s) 1025
HhaI GCGC 1 cut(s) 899
Hin1II CATG 9 cut(s) 104, 266, 308, 361, 743, 790, 958, 1030, 1043
Hin6I GCGC 1 cut(s) 897
HinP1I GCGC 1 cut(s) 897
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 6 cut(s) 148, 191, 361, 394, 793, 997
HpaI GTTAAC 1 cut(s) 271
HpaII CCGG 2 cut(s) 81, 843
HphI GGTGA 4 cut(s) 455, 742, 976, 992
Hpy166II GTNNAC 2 cut(s) 271, 670
Hpy188I TCNGA 4 cut(s) 36, 160, 253, 798
Hpy188III TCNNGA 4 cut(s) 319, 358, 724, 787
Hpy8I GTNNAC 2 cut(s) 271, 670
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 139, 550, 897
HpyCH4III ACNGT 4 cut(s) 198, 232, 732, 784
HpyCH4V TGCA 6 cut(s) 214, 428, 541, 563, 580, 715
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 211, 586, 807
HpyF3I CTNAG 5 cut(s) 189, 348, 396, 622, 722
Hsp92II CATG 9 cut(s) 104, 266, 308, 361, 743, 790, 958, 1030, 1043
HspAI GCGC 1 cut(s) 897
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 6 cut(s) 31, 71, 479, 516, 718, 847
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 2 cut(s) 293, 845
Lsp1109I GCAGC 2 cut(s) 414, 613
LweI GCATC 2 cut(s) 244, 415
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 3 cut(s) 246, 531, 894
MaeIII GTNAC 2 cut(s) 192, 526
MalI GATC 6 cut(s) 33, 73, 481, 518, 720, 849
MboI GATC 6 cut(s) 31, 71, 479, 516, 718, 847
MboII GAAGA 5 cut(s) 124, 474, 644, 673, 802
MflI RGATCY 3 cut(s) 71, 479, 718
MhlI GDGCHC 1 cut(s) 41
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 1 cut(s) 925
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 4 cut(s) 200, 370, 388, 991
MmeI TCCRAC 2 cut(s) 85, 1002
MnlI CCTC 5 cut(s) 357, 691, 710, 747, 1014
Mox20I TGGCCA 1 cut(s) 99
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 99
MseI TTAA 4 cut(s) 21, 270, 300, 551
MslI CAYNNNNRTG 1 cut(s) 785
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 2 cut(s) 81, 843
MspR9I CCNGG 2 cut(s) 492, 704
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 2 cut(s) 492, 704
MwoI GCNNNNNNNGC 4 cut(s) 90, 211, 586, 807
NdeI CATATG 1 cut(s) 118
NdeII GATC 6 cut(s) 31, 71, 479, 516, 718, 847
NheI GCTAGC 1 cut(s) 893
NlaIII CATG 9 cut(s) 104, 266, 308, 361, 743, 790, 958, 1030, 1043
NlaIV GGNNCC 3 cut(s) 85, 289, 841
NmuCI GTSAC 2 cut(s) 192, 526
PagI TCATGA 2 cut(s) 357, 786
PceI AGGCCT 1 cut(s) 830
PciSI GCTCTTC 1 cut(s) 656
PctI GAATGC 1 cut(s) 775
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 2 cut(s) 148, 793
PkrI GCNGC 3 cut(s) 404, 628, 989
PleI GAGTC 4 cut(s) 199, 369, 388, 991
PpsI GAGTC 4 cut(s) 199, 369, 388, 991
Psp6I CCWGG 2 cut(s) 490, 702
PspGI CCWGG 2 cut(s) 490, 702
PspN4I GGNNCC 3 cut(s) 85, 289, 841
PspPI GGNCC 1 cut(s) 83
PstI CTGCAG 1 cut(s) 543
PsuI RGATCY 3 cut(s) 71, 479, 718
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 3 cut(s) 806, 920, 959
RsaNI GTAC 3 cut(s) 805, 919, 958
RseI CAYNNNNRTG 1 cut(s) 785
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 4 cut(s) 21, 270, 300, 551
SatI GCNGC 3 cut(s) 403, 627, 988
Sau3AI GATC 6 cut(s) 31, 71, 479, 516, 718, 847
Sau96I GGNCC 1 cut(s) 83
SchI GAGTC 4 cut(s) 200, 370, 388, 991
ScrFI CCNGG 2 cut(s) 492, 704
SduI GDGCHC 1 cut(s) 41
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 2 cut(s) 244, 415
SfcI CTRYAG 2 cut(s) 220, 539
SmiMI CAYNNNNRTG 1 cut(s) 785
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 1 cut(s) 925
SseBI AGGCCT 1 cut(s) 830
SsiI CCGC 5 cut(s) 42, 139, 166, 743, 987
SspMI CTAG 3 cut(s) 246, 531, 894
StuI AGGCCT 1 cut(s) 830
StyD4I CCNGG 2 cut(s) 490, 702
TaaI ACNGT 4 cut(s) 198, 232, 732, 784
TaqI TCGA 2 cut(s) 151, 905
TaqII GACCGA 1 cut(s) 690
TasI AATT 1 cut(s) 925
TatI WGTACW 2 cut(s) 918, 957
TauI GCSGC 1 cut(s) 990
TfiI GAWTC 2 cut(s) 148, 793
Tru1I TTAA 4 cut(s) 21, 270, 300, 551
Tru9I TTAA 4 cut(s) 21, 270, 300, 551
TscAI CASTG 6 cut(s) 94, 201, 237, 298, 787, 952
TseFI GTSAC 2 cut(s) 192, 526
TseI GCWGC 2 cut(s) 402, 626
Tsp45I GTSAC 2 cut(s) 192, 526
TspDTI ATGAA 4 cut(s) 172, 803, 929, 1043
TspRI CASTG 6 cut(s) 94, 201, 237, 298, 787, 952
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 3 cut(s) 246, 531, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.