Rroxscaffold_157G00438320

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000157
OG0015101
Physical Location & Seq
Reverse (-)
12886 .. 14329
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_157G00438320.1

Sequence Viewer

Length: 1158 bp
ATGCCAGTAAGACCAGCAATGGCGTCTATCTCTTTCAGTACCATCACCACCTTCTTCATCTTCGTCTTCATCCTCACCTCTTCCTCTTGTTCTGCCTCTACTCCTTCAACTAAGACAACCCCACATCCCTTCAAGAAAATCTATGCCTTTGGTGACTCCTTCACTGACACAGGCAACACAAGATCTGTCTCCGGCCCCAGTGGCTTTGGCCATGTTGGAAACCCCCCATATGGCATTACCTTCTTCCACCGCCCGACTAATCGATACTCCGATGGGCGGTTAGTCATAGACTTCATAGCTGACTCACTGTCCTTGCCTTACTTGCAACCCTACAGAAACATAGTGAGCAATGCTTCTAGCTCTGATGCTTCTCATGGGGTTAACTTTGCTGTTGCTGGCTCCACTGCTATTAACCATGACTTCTTTGTCAAGAACAACCTTACACTTGATATCACTCCTCAGTCTATTATGAGTCAGCTGAACTGGTTCAACAAGTTCTCGAAGACTCAGGGCAGCAAAGCAGGAAATTTTGATGATGCACTTTTTTGGGTTGGTGAAATTGGGGTCAATGATTATGCTTACACTCTTGGATCTTCCATACCAGGTGCTACCATCCAAAAGCTTGGGATCAGTAGTGTCACTAGTTTTCTGCAGGCATTGTTAAAGAAGGGTGCAAAGTATGTGGTTGTGCAAGGTCTGCCGATTTCGGGGTGCTTGCCATTAGCAATGAGCTTAGCTGCTGAAGACGATAGAGACAGCATAGGATGTGTGAAGAGCGTGAACGACCAAACCTCTGCTCACAACCTTGTCCTCCAGGCCAAGTTGCAAGATCTCAGGAAACAGTTTCCTCATGCGGTAATCACCTATGCTGATTACTGGAATGCCTATTACACTGTCATGAAGAATCCGAGCCAGTACGGCTTCAAGGAGAGCTTCAAGGCCTGCTGTGGCTCCGGTGATCCCTACAACTTCAATGTATTTGCCGTGTGTGGCACACCTTCTGCTAGTGCCTGCTCGAACCCAAGCCAGTACATAAATTGGGATGGAGTTCATCTCACTGAAGCCATGTACAAGGTGATTACTAATATGTTTGTAAGCGGCAAAGCGACTCACCCTCCATTCAGCTACTTGTTGGACATGAAACTGCGTCATGGATGA

Protein Analysis

385

Amino Acids

41.93

Weight (kDa)

8.69

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 361 5.5e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015101)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48460
fragaria_vesca FvH4_5g07260
malus_domestica MD06G1157800.v1.1
prunus_persica Prupe.5G157800_v2.0.a1 Prupe.I001600_v2.0.a1
pyrus_communis pycom06g14090 pycom14g13710
rosa_chinensis RchiOBHm_Chr7g0191371
rosa_laevigata RLG00000004461
rosa_multiflora Rmu_co8465791.1_g000001
rosa_roxburghii Rroxscaffold_157G00438320
rosa_rugosa Rorug06G0510300
rosa_samantha Rh7AG117600 Rh7BG119700 Rh7CG122700 Rh7DG120800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AciI CCGC 4 cut(s) 250, 277, 854, 1098
AclWI GGATC 3 cut(s) 598, 635, 953
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 2 cut(s) 762, 1080
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 4 cut(s) 40, 917, 1031, 1070
AfiI CCNNNNNNNGG 3 cut(s) 230, 276, 707
AgsI TTSAA 6 cut(s) 108, 133, 490, 925, 937, 973
AhdI GACNNNNNGTC 1 cut(s) 307
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 2 cut(s) 601, 813
AluBI AGCT 8 cut(s) 299, 360, 478, 622, 732, 737, 933, 1125
AluI AGCT 8 cut(s) 299, 360, 478, 622, 732, 737, 933, 1125
Alw26I GTCTC 2 cut(s) 193, 747
AlwI GGATC 3 cut(s) 598, 635, 953
AoxI GGCC 4 cut(s) 193, 208, 816, 939
ApeKI GCWGC 2 cut(s) 513, 737
ApoI RAATTY 1 cut(s) 526
Asp700I GAANNNNTTC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 8 cut(s) 37, 67, 164, 566, 853, 968, 1087, 1103
BalI TGGCCA 1 cut(s) 210
BbsI GAAGAC 3 cut(s) 58, 509, 750
BbvI GCAGC 2 cut(s) 525, 724
BccI CCATC 4 cut(s) 50, 266, 620, 1037
BceAI ACGGC 2 cut(s) 934, 968
BciT130I CCWGG 2 cut(s) 603, 815
BcoDI GTCTC 2 cut(s) 193, 747
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 3 cut(s) 357, 642, 1005
BfmI CTRYAG 2 cut(s) 331, 650
BglI GCCNNNNNGGC 2 cut(s) 201, 918
BglII AGATCT 2 cut(s) 182, 829
BisI GCNGC 3 cut(s) 514, 738, 1099
BlpI GCTNAGC 1 cut(s) 733
BlsI GCNGC 3 cut(s) 515, 739, 1100
Bme1390I CCNGG 2 cut(s) 603, 815
BmeRI GACNNNNNGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 3 cut(s) 196, 400, 952
BmrFI CCNGG 2 cut(s) 603, 815
BmrI ACTGGG 1 cut(s) 192
BmsI GCATC 2 cut(s) 355, 526
BmuI ACTGGG 1 cut(s) 192
BpiI GAAGAC 3 cut(s) 58, 509, 750
BplI GAGNNNNNCTC 2 cut(s) 1038, 1070
BpmI CTGGAG 1 cut(s) 797
Bpu1102I GCTNAGC 1 cut(s) 733
Bsa29I ATCGAT 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 23
BsaWI WCCGGW 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 1099, 1129
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 276, 707
Bse1I ACTGG 6 cut(s) 5, 198, 488, 881, 913, 1027
Bse3DI GCAATG 3 cut(s) 24, 355, 732
BseBI CCWGG 2 cut(s) 603, 815
BseCI ATCGAT 1 cut(s) 262
BseGI GGATG 5 cut(s) 69, 124, 612, 770, 1048
BseLI CCNNNNNNNGG 3 cut(s) 230, 276, 707
BseMI GCAATG 3 cut(s) 24, 355, 732
BseMII CTCAG 3 cut(s) 473, 521, 847
BseNI ACTGG 6 cut(s) 5, 198, 488, 881, 913, 1027
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 2 cut(s) 525, 724
BshFI GGCC 4 cut(s) 195, 210, 818, 941
BshVI ATCGAT 1 cut(s) 262
BsiSI CCGG 2 cut(s) 192, 954
BslI CCNNNNNNNGG 3 cut(s) 230, 276, 707
BsmAI GTCTC 2 cut(s) 193, 747
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 4 cut(s) 195, 210, 818, 941
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 5 cut(s) 182, 590, 627, 829, 958
Bsp1720I GCTNAGC 1 cut(s) 733
BspACI CCGC 4 cut(s) 250, 277, 854, 1098
BspANI GGCC 4 cut(s) 195, 210, 818, 941
BspCNI CTCAG 3 cut(s) 472, 520, 846
BspDI ATCGAT 1 cut(s) 262
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 3 cut(s) 196, 400, 952
BspMAI CTGCAG 1 cut(s) 654
BspPI GGATC 3 cut(s) 598, 635, 953
BspQI GCTCTTC 1 cut(s) 767
BsrDI GCAATG 3 cut(s) 24, 355, 732
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 6 cut(s) 5, 198, 488, 881, 913, 1027
BssMI GATC 5 cut(s) 182, 590, 627, 829, 958
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 603, 815
Bst4CI ACNGT 3 cut(s) 309, 843, 895
Bst6I CTCTTC 2 cut(s) 85, 767
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 697
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 5 cut(s) 397, 654, 716, 943, 1012
BstDEI CTNAG 5 cut(s) 111, 459, 507, 733, 833
BstF5I GGATG 5 cut(s) 69, 124, 612, 770, 1048
BstKTI GATC 5 cut(s) 185, 593, 630, 832, 961
BstMAI GTCTC 2 cut(s) 193, 747
BstMBI GATC 5 cut(s) 182, 590, 627, 829, 958
BstMWI GCNNNNNNNGC 4 cut(s) 201, 322, 697, 918
BstNI CCWGG 2 cut(s) 603, 815
BstSCI CCNGG 2 cut(s) 601, 813
BstSFI CTRYAG 2 cut(s) 331, 650
BstV1I GCAGC 2 cut(s) 525, 724
BstV2I GAAGAC 3 cut(s) 58, 509, 750
BstX2I RGATCY 3 cut(s) 182, 590, 829
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 3 cut(s) 182, 590, 829
Bsu15I ATCGAT 1 cut(s) 262
BsuRI GGCC 4 cut(s) 195, 210, 818, 941
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 5 cut(s) 69, 124, 612, 770, 1048
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 6 cut(s) 162, 205, 305, 402, 891, 1056
Cac8I GCNNGC 5 cut(s) 397, 654, 716, 943, 1012
CciI TCATGA 1 cut(s) 897
Cfr13I GGNCC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 262
CseI GACGC 2 cut(s) 12, 1136
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 4 cut(s) 39, 916, 1030, 1069
CspCI CAANNNNNGTGG 2 cut(s) 663, 698
CviAII CATG 8 cut(s) 212, 374, 416, 851, 898, 1066, 1138, 1151
CviQI GTAC 4 cut(s) 39, 916, 1030, 1069
DdeI CTNAG 5 cut(s) 111, 459, 507, 733, 833
DpnI GATC 5 cut(s) 184, 592, 629, 831, 960
DpnII GATC 5 cut(s) 182, 590, 627, 829, 958
DrdI GACNNNNNNGTC 1 cut(s) 425
DriI GACNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 85, 767
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 2 cut(s) 85, 767
Eco147I AGGCCT 1 cut(s) 941
Eco32I GATATC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 762, 1080
EcoRII CCWGG 2 cut(s) 601, 813
EcoRV GATATC 1 cut(s) 451
FaeI CATG 8 cut(s) 215, 377, 419, 854, 901, 1069, 1141, 1154
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FatI CATG 8 cut(s) 211, 373, 415, 850, 897, 1065, 1137, 1150
FauNDI CATATG 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 514, 738, 1099
FokI GGATG 5 cut(s) 56, 111, 599, 777, 1055
Fsp4HI GCNGC 3 cut(s) 514, 738, 1099
FspBI CTAG 3 cut(s) 357, 642, 1005
GluI GCNGC 3 cut(s) 514, 738, 1099
GsuI CTGGAG 1 cut(s) 797
HaeIII GGCC 4 cut(s) 195, 210, 818, 941
HapII CCGG 2 cut(s) 192, 954
HgaI GACGC 2 cut(s) 12, 1136
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 8 cut(s) 215, 377, 419, 854, 901, 1069, 1141, 1154
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 6 cut(s) 155, 302, 472, 505, 904, 1108
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 2 cut(s) 192, 954
HphI GGTGA 8 cut(s) 37, 67, 164, 566, 853, 968, 1087, 1103
Hpy166II GTNNAC 2 cut(s) 382, 781
Hpy188I TCNGA 3 cut(s) 271, 364, 909
Hpy188III TCNNGA 5 cut(s) 133, 430, 499, 835, 898
Hpy8I GTNNAC 2 cut(s) 382, 781
HpyAV CCTTC 7 cut(s) 61, 114, 139, 169, 250, 661, 1008
HpyCH4III ACNGT 3 cut(s) 309, 843, 895
HpyCH4V TGCA 6 cut(s) 325, 539, 652, 674, 691, 826
HpyF10VI GCNNNNNNNGC 4 cut(s) 201, 322, 697, 918
HpyF3I CTNAG 5 cut(s) 111, 459, 507, 733, 833
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 8 cut(s) 215, 377, 419, 854, 901, 1069, 1141, 1154
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 5 cut(s) 182, 590, 627, 829, 958
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 2 cut(s) 404, 956
Lsp1109I GCAGC 2 cut(s) 525, 724
LweI GCATC 2 cut(s) 355, 526
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 3 cut(s) 357, 642, 1005
MaeIII GTNAC 2 cut(s) 152, 637
MalI GATC 5 cut(s) 184, 592, 629, 831, 960
MboI GATC 5 cut(s) 182, 590, 627, 829, 958
MflI RGATCY 3 cut(s) 182, 590, 829
MlsI TGGCCA 1 cut(s) 210
MluCI AATT 3 cut(s) 526, 558, 1036
MluNI TGGCCA 1 cut(s) 210
MlyI GAGTC 5 cut(s) 149, 296, 481, 499, 1102
MmeI TCCRAC 2 cut(s) 196, 1113
MnlI CCTC 9 cut(s) 83, 88, 94, 106, 468, 802, 821, 858, 1125
Mox20I TGGCCA 1 cut(s) 210
MroXI GAANNNNTTC 1 cut(s) 485
MscI TGGCCA 1 cut(s) 210
MseI TTAA 3 cut(s) 381, 411, 662
MslI CAYNNNNRTG 1 cut(s) 896
Msp20I TGGCCA 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 2 cut(s) 192, 954
MspR9I CCNGG 2 cut(s) 603, 815
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 603, 815
MwoI GCNNNNNNNGC 4 cut(s) 201, 322, 697, 918
NdeI CATATG 1 cut(s) 229
NdeII GATC 5 cut(s) 182, 590, 627, 829, 958
NlaIII CATG 8 cut(s) 215, 377, 419, 854, 901, 1069, 1141, 1154
NlaIV GGNNCC 3 cut(s) 196, 400, 952
NmuCI GTSAC 2 cut(s) 152, 637
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 1 cut(s) 941
PciSI GCTCTTC 1 cut(s) 767
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 1 cut(s) 904
PkrI GCNGC 3 cut(s) 515, 739, 1100
PleI GAGTC 5 cut(s) 149, 296, 480, 499, 1102
PpsI GAGTC 5 cut(s) 149, 296, 480, 499, 1102
Psp6I CCWGG 2 cut(s) 601, 813
PspGI CCWGG 2 cut(s) 601, 813
PspN4I GGNNCC 3 cut(s) 196, 400, 952
PspPI GGNCC 1 cut(s) 194
PstI CTGCAG 1 cut(s) 654
PsuI RGATCY 3 cut(s) 182, 590, 829
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 4 cut(s) 40, 917, 1031, 1070
RsaNI GTAC 4 cut(s) 39, 916, 1030, 1069
RseI CAYNNNNRTG 1 cut(s) 896
SapI GCTCTTC 1 cut(s) 767
SaqAI TTAA 3 cut(s) 381, 411, 662
SatI GCNGC 3 cut(s) 514, 738, 1099
Sau3AI GATC 5 cut(s) 182, 590, 627, 829, 958
Sau96I GGNCC 1 cut(s) 194
SchI GAGTC 5 cut(s) 149, 296, 481, 499, 1102
ScrFI CCNGG 2 cut(s) 603, 815
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 355, 526
SfcI CTRYAG 2 cut(s) 331, 650
SmiMI CAYNNNNRTG 1 cut(s) 896
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 3 cut(s) 526, 558, 1036
SseBI AGGCCT 1 cut(s) 941
SsiI CCGC 4 cut(s) 250, 277, 854, 1098
SspMI CTAG 3 cut(s) 357, 642, 1005
StuI AGGCCT 1 cut(s) 941
StyD4I CCNGG 2 cut(s) 601, 813
TaaI ACNGT 3 cut(s) 309, 843, 895
TaqI TCGA 3 cut(s) 262, 500, 1016
TasI AATT 3 cut(s) 526, 558, 1036
TatI WGTACW 2 cut(s) 1029, 1068
TauI GCSGC 1 cut(s) 1101
TfiI GAWTC 1 cut(s) 904
Tru1I TTAA 3 cut(s) 381, 411, 662
Tru9I TTAA 3 cut(s) 381, 411, 662
TscAI CASTG 6 cut(s) 169, 205, 312, 409, 898, 1063
TseFI GTSAC 2 cut(s) 152, 637
TseI GCWGC 2 cut(s) 513, 737
Tsp45I GTSAC 2 cut(s) 152, 637
TspDTI ATGAA 6 cut(s) 46, 58, 283, 914, 1040, 1154
TspRI CASTG 6 cut(s) 169, 205, 312, 409, 898, 1063
XapI RAATTY 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 3 cut(s) 357, 642, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.