AT3G49320

Uncharacterised protein family (UPF0160)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0012304
Physical Location & Seq
Reverse (-)
18288172 .. 18290672
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49320.2

Sequence Viewer

Length: 1065 bp
ATGTTTGGGTCAAGAGGTCTCTGTTGCAGCAGAATCTGGAATCGTTCTCTGTTTCTCAAACGTTCTTCAAACTTCCGAGCTAGTTTCTCTACGAAACGTGTTGGGACCCATAATGGAACATTCCACTGCGACGAAGCTTTAGCTTGCTTCATCCTTCGTCGTTCCAATAGATTCTCCGATGCTCAAATCGTCCGAACCAGAGATCATCAGGTTTTGGAGAAGCTTGATGCGGCACTTGATGTTGGAGGTGTGTATGATCCTCAGAGTGAACGTTATGACCATCACCAGAAAGGATTTAGTGAAGTGTTTGGCCTTGGGTTTAATACTAAACTCAGTAGTGCTGGGCTTGTCTATAAGCATTATGGATTGGAAATAATTTCCAAGGAGCTTCAACTTGAGCAGAGACATCCTGATGTGTTTCGATTGTTTCTAGCTGTGTACAAAAACTTCATTGAGGCAGTAGATGCTTTAGACAATGGTATCCATCAATATGATACTGACCAACCTCCAAGATATGTAAACAATACTAGCCTGGGGCATAGGATTGGAAGATTGAACTTAGACTGGATTGAACCTGATCAGTCTAGTGCCAAAGAAGATGAAGCCTTTCATCGGGCAATGGAGCTTGCTGGCTCTGAGTTCTTGGAGTGTGTTCATTTTCACGCGAAATCGTGGTTACCAGCTCGGTCAATTGTTATGGAGTGTCTTGCAAAACGGTATGATATAGACTCCAGTGGAGAAATTATGAAGCTCAGTAAACAATGCCCATGGAAACTCCATATATTCGAGCTCGAGGAAGAAATGAAGATTGATCCTCCAATAAAATATGTTCTTTACCAGGATGATAGAAGCGAAAACTGGAGAATACAAGCAGTTTCGGTTTCACCAGAGAGGTTTGAGAGCCGTAAAGCTTTACCATTAGCATGGAGAGGTTTAGAAAAGGAGAAGCTCTCAGAGGAAAGCTCAATTCCACGCTGTGTTTTTGTGCATATGAGTGGTTTCATTGGTGCAAACCAAACCTATGAAGGTGCCTTAGCAATGGCAAGAGCTTCTTTGATGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0160

Protein Analysis

354

Amino Acids

40.69

Weight (kDa)

6.71

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MYG1_exonuc PF03690 34 - 257 1.9e-87 Uncharacterised protein family (UPF0160)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49320 AT3G49320 AT5G41970
fragaria_vesca FvH4_5g11020
malus_domestica MD06G1117500.v1.1 MD14G1124700.v1.1
prunus_persica Prupe.5G128600_v2.0.a1
pyrus_communis pycom07g04680 pycom14g11040
rosa_chinensis RchiOBHm_Chr7g0186791
rosa_laevigata RLG00000004815
rosa_multiflora Rmu_ssc0000024.1_g000044
rosa_roxburghii Rroxscaffold_3G00267820
rosa_rugosa Rorug06G0476700
rosa_samantha Rh7AG063800 Rh7BG080300 Rh7CG083700 Rh7DG084600
rosa_wichuraiana Rw7G007260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1028
AccII CGCG 1 cut(s) 665
AciI CCGC 1 cut(s) 230
AclI AACGTT 2 cut(s) 61, 271
AclWI GGATC 2 cut(s) 251, 806
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 1 cut(s) 612
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 4 cut(s) 69, 392, 556, 572
AjnI CCWGG 2 cut(s) 531, 837
Alw21I GWGCWC 1 cut(s) 792
Alw26I GTCTC 2 cut(s) 23, 397
AlwI GGATC 2 cut(s) 251, 806
AlwNI CAGNNNCTG 1 cut(s) 36
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 1 cut(s) 27
Asp700I GAANNNNTTC 1 cut(s) 606
AspS9I GGNCC 1 cut(s) 105
AsuHPI GGTGA 2 cut(s) 275, 876
AvaI CYCGRG 1 cut(s) 791
AvaII GGWCC 1 cut(s) 105
BaeI ACNNNNGTAYC 2 cut(s) 463, 496
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 792
BarI GAAGNNNNNNTAC 2 cut(s) 431, 463
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 1 cut(s) 39
BccI CCATC 3 cut(s) 288, 492, 1051
BceAI ACGGC 1 cut(s) 888
BciT130I CCWGG 2 cut(s) 533, 839
BciVI GTATCC 1 cut(s) 491
BclI TGATCA 1 cut(s) 577
BcoDI GTCTC 2 cut(s) 23, 397
BfaI CTAG 4 cut(s) 81, 431, 528, 585
BfuI GTATCC 1 cut(s) 491
BisI GCNGC 2 cut(s) 28, 231
BlsI GCNGC 2 cut(s) 29, 232
Bme1390I CCNGG 2 cut(s) 533, 839
Bme18I GGWCC 1 cut(s) 105
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 105
BmiI GGNNCC 3 cut(s) 106, 107, 1030
BmrFI CCNGG 2 cut(s) 533, 839
BmsI GCATC 3 cut(s) 169, 217, 454
BplI GAGNNNNNCTC 4 cut(s) 935, 967, 947, 979
BpmI CTGGAG 2 cut(s) 715, 880
Bpu10I CCTNAGC 1 cut(s) 1033
BpuEI CTTGAG 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 313, 381, 532, 767
Bsc4I CCNNNNNNNGG 1 cut(s) 612
Bse1I ACTGG 3 cut(s) 569, 732, 863
Bse3DI GCAATG 2 cut(s) 624, 1044
BseBI CCWGG 2 cut(s) 533, 839
BseDI CCNNGG 4 cut(s) 313, 381, 532, 767
BseGI GGATG 3 cut(s) 150, 406, 847
BseLI CCNNNNNNNGG 1 cut(s) 612
BseMI GCAATG 2 cut(s) 624, 1044
BseMII CTCAG 5 cut(s) 275, 346, 627, 766, 966
BseNI ACTGG 3 cut(s) 569, 732, 863
BseXI GCAGC 1 cut(s) 39
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 665
BshFI GGCC 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 1028
BsiHKAI GWGCWC 1 cut(s) 792
BsiHKCI CYCGRG 1 cut(s) 791
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 612
BsmAI GTCTC 2 cut(s) 23, 397
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 23
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 792
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 4 cut(s) 202, 256, 577, 811
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 1 cut(s) 230
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 5 cut(s) 274, 345, 628, 765, 965
BspFNI CGCG 1 cut(s) 665
BspLI GGNNCC 3 cut(s) 106, 107, 1030
BspPI GGATC 2 cut(s) 251, 806
BspT107I GGYRCC 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 624, 1044
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 3 cut(s) 569, 732, 863
BssECI CCNNGG 4 cut(s) 313, 381, 532, 767
BssMI GATC 4 cut(s) 202, 256, 577, 811
BssT1I CCWWGG 3 cut(s) 313, 381, 767
Bst2UI CCWGG 2 cut(s) 533, 839
Bst4CI ACNGT 1 cut(s) 717
BstAPI GCANNNNNTGC 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 145, 627, 631
BstDEI CTNAG 8 cut(s) 261, 332, 559, 636, 752, 952, 1033, 1062
BstDSI CCRYGG 1 cut(s) 767
BstEII GGTNACC 1 cut(s) 675
BstF5I GGATG 3 cut(s) 150, 406, 847
BstFNI CGCG 1 cut(s) 665
BstKTI GATC 4 cut(s) 205, 259, 580, 814
BstMAI GTCTC 2 cut(s) 23, 397
BstMBI GATC 4 cut(s) 202, 256, 577, 811
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 2 cut(s) 533, 839
BstPI GGTNACC 1 cut(s) 675
BstSCI CCNGG 2 cut(s) 531, 837
BstUI CGCG 1 cut(s) 665
BstV1I GCAGC 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 924
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 3 cut(s) 150, 406, 847
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 2 cut(s) 124, 739
Cac8I GCNNGC 3 cut(s) 145, 627, 631
CaiI CAGNNNCTG 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 105
Csp6I GTAC 1 cut(s) 439
CviAII CATG 2 cut(s) 768, 924
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 8 cut(s) 261, 332, 559, 636, 752, 952, 1033, 1062
DpnI GATC 4 cut(s) 204, 258, 579, 813
DpnII GATC 4 cut(s) 202, 256, 577, 811
DraIII CACNNNGTG 1 cut(s) 977
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 3 cut(s) 313, 381, 767
Eco24I GRGCYC 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 790
Eco88I CYCGRG 1 cut(s) 791
Eco91I GGTNACC 1 cut(s) 675
EcoICRI GAGCTC 1 cut(s) 790
EcoO109I RGGNCCY 1 cut(s) 105
EcoO65I GGTNACC 1 cut(s) 675
EcoRII CCWGG 2 cut(s) 531, 837
EcoT14I CCWWGG 3 cut(s) 313, 381, 767
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 3 cut(s) 313, 381, 767
FaeI CATG 2 cut(s) 771, 927
FalI AAGNNNNNCTT 1 cut(s) 1036
FaqI GGGAC 1 cut(s) 118
FatI CATG 2 cut(s) 767, 923
FauNDI CATATG 1 cut(s) 990
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 2 cut(s) 28, 231
FokI GGATG 3 cut(s) 137, 393, 854
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 2 cut(s) 28, 231
FspBI CTAG 4 cut(s) 81, 431, 528, 585
GluI GCNGC 2 cut(s) 28, 231
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 715, 880
HaeIII GGCC 1 cut(s) 312
Hin1II CATG 2 cut(s) 771, 927
HindIII AAGCTT 3 cut(s) 135, 221, 909
HinfI GANTC 4 cut(s) 33, 40, 171, 728
HphI GGTGA 2 cut(s) 275, 876
Hpy166II GTNNAC 4 cut(s) 269, 439, 520, 758
Hpy188I TCNGA 6 cut(s) 77, 178, 194, 264, 637, 955
Hpy188III TCNNGA 3 cut(s) 12, 37, 410
Hpy8I GTNNAC 4 cut(s) 269, 439, 520, 758
Hpy99I CGWCG 2 cut(s) 134, 162
HpyAV CCTTC 2 cut(s) 164, 1019
HpyCH4III ACNGT 1 cut(s) 717
HpyCH4IV ACGT 3 cut(s) 61, 97, 271
HpyCH4V TGCA 4 cut(s) 27, 710, 988, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 8 cut(s) 261, 332, 559, 636, 752, 952, 1033, 1062
HpySE526I ACGT 3 cut(s) 61, 97, 271
Hsp92II CATG 2 cut(s) 771, 927
KflI GGGWCCC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 202, 256, 577, 811
LmnI GCTCC 2 cut(s) 385, 622
Lsp1109I GCAGC 1 cut(s) 39
LweI GCATC 3 cut(s) 169, 217, 454
MaeI CTAG 4 cut(s) 81, 431, 528, 585
MaeII ACGT 3 cut(s) 61, 97, 271
MaeIII GTNAC 1 cut(s) 675
MalI GATC 4 cut(s) 204, 258, 579, 813
MboI GATC 4 cut(s) 202, 256, 577, 811
MboII GAAGA 5 cut(s) 57, 561, 608, 809, 817
MfeI CAATTG 1 cut(s) 690
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 4 cut(s) 375, 690, 741, 966
MlyI GAGTC 1 cut(s) 722
MmeI TCCRAC 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 606
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 4 cut(s) 411, 489, 922, 993
MspR9I CCNGG 2 cut(s) 533, 839
MunI CAATTG 1 cut(s) 690
MvaI CCWGG 2 cut(s) 533, 839
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 1 cut(s) 464
NcoI CCATGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 990
NdeII GATC 4 cut(s) 202, 256, 577, 811
NlaIII CATG 2 cut(s) 771, 927
NlaIV GGNNCC 3 cut(s) 106, 107, 1030
PaeR7I CTCGAG 1 cut(s) 791
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 33, 40, 171
PkrI GCNGC 2 cut(s) 29, 232
PleI GAGTC 1 cut(s) 722
PpsI GAGTC 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 105
Psp124BI GAGCTC 1 cut(s) 792
Psp1406I AACGTT 2 cut(s) 61, 271
Psp5II RGGWCCY 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 531, 837
PspEI GGTNACC 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 531, 837
PspN4I GGNNCC 3 cut(s) 106, 107, 1030
PspPI GGNCC 1 cut(s) 105
PspPPI RGGWCCY 1 cut(s) 105
PspXI VCTCGAGB 1 cut(s) 791
PstNI CAGNNNCTG 1 cut(s) 36
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 4 cut(s) 411, 489, 922, 993
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 2 cut(s) 28, 231
Sau3AI GATC 4 cut(s) 202, 256, 577, 811
Sau96I GGNCC 1 cut(s) 105
SchI GAGTC 1 cut(s) 722
ScrFI CCNGG 2 cut(s) 533, 839
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 3 cut(s) 169, 217, 454
Sfr274I CTCGAG 1 cut(s) 791
SinI GGWCC 1 cut(s) 105
SlaI CTCGAG 1 cut(s) 791
SmiMI CAYNNNNRTG 4 cut(s) 411, 489, 922, 993
SmlI CTYRAG 2 cut(s) 395, 791
SmoI CTYRAG 2 cut(s) 395, 791
Sse9I AATT 4 cut(s) 375, 690, 741, 966
SsiI CCGC 1 cut(s) 230
SspMI CTAG 4 cut(s) 81, 431, 528, 585
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 2 cut(s) 531, 837
StyI CCWWGG 3 cut(s) 313, 381, 767
TaaI ACNGT 1 cut(s) 717
TaiI ACGT 3 cut(s) 64, 100, 274
TaqI TCGA 3 cut(s) 421, 786, 792
TaqII GACCGA 1 cut(s) 675
TasI AATT 4 cut(s) 375, 690, 741, 966
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 3 cut(s) 33, 40, 171
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 131, 739
TseI GCWGC 1 cut(s) 27
TspDTI ATGAA 9 cut(s) 139, 439, 599, 615, 644, 761, 818, 991, 1038
TspRI CASTG 2 cut(s) 131, 739
VpaK11BI GGWCC 1 cut(s) 105
XhoI CTCGAG 1 cut(s) 791
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 4 cut(s) 81, 431, 528, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.