MD14G1124700.v1.1

UPF0160 protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012304
Physical Location & Seq
Reverse (-)
20017922 .. 20020709
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1124700.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGCTTCCGCTAGTAGGCAGAGGGTTTAAGCACACACTCCCATTCGTCTTCCCCGCCTACCACCACCGCCTACACCTCCACCTCTCCCGCTCTCTCATGGCCGCCGCTACTTCACCTAAGCGAGTGGGGACCCACAACGGCAGCTTCCACTGCGACGAAGCCCTCGGCTGCTTCATGATTCGCCTCACAGACAAGTTCTCCAACGCCGAAATCGTCCGCACTCGCGACCCCCAGGTTTTGGAGGTTCTGGATGCTGTGCTTGACGTTGGGGGTGTGTATGATTCTAGTCGGGACCGATACGATCATCACCAGAAGGGTTTCGAGGAGGTTTTCGGGCACGGGTTTAAGACCAAGCTGAGCAGTGCTGGTCTGGTTTACAAGCATTTCGGGAAGGAAATAATAGCGAAGGAGCTTCAGGTCGATGAAGGTCACCTCGATGTGCACCGGTTGTTCTTGGCTGTGTACAAAAGCTTTATGGAGGCAATTGATGCTGTTGACAACGGGATCAATCGGTATGATACGGACCAGCCTCCAAGATATGTGAATAATACACACTTGTCTTCAAGGGTGGGCAGGTTGAATTTAGATTGGATAGATCCTGATCAATCATCTGAAAAGGAGAACGAAGCCTTTCAAAGAGCAATGGAACTTGCGGGCAGCGAATTTTTAAGTAGTGTCCGTTTTCATGCAAAATCATGGTTACCAGCTCGATCTATCGTGATGGAGTGTCTCTTGGCAAGATGGAGTGTTGATCCAAGTGGTGAAATCATGGTTTTGAATCGATTTTGCCCTTGGAAGCTTCATTTATTTGAACTTGAAGAGGAAATGAAGATTGAGCCGCCCATCAAATATGTTCTCTATCAGGATGATAGAAGCAAACACTGGAGAGTTCAAGCGGTTGCAGTCACTCCTGATAGATTTGAGAGCCGAAAGGCTCTTGCATCACAATGGCGGGGGCTTAGGGACGAGGAGCTCTCAAAGGAGACTGGGATTCCTGGATGTGTTTTCGTTCACATGAGTGGATTTATTGGAGGAAATCAAAGTTATGAAGGTGCTCTTGCCATGGCAAAAGGGAGTTTGAAGTCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0160

Protein Analysis

364

Amino Acids

41.32

Weight (kDa)

6.53

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MYG1_exonuc PF03690 42 - 360 2.3e-137 Uncharacterised protein family (UPF0160)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49320 AT3G49320 AT5G41970
fragaria_vesca FvH4_5g11020
malus_domestica MD06G1117500.v1.1 MD14G1124700.v1.1
prunus_persica Prupe.5G128600_v2.0.a1
pyrus_communis pycom07g04680 pycom14g11040
rosa_chinensis RchiOBHm_Chr7g0186791
rosa_laevigata RLG00000004815
rosa_multiflora Rmu_ssc0000024.1_g000044
rosa_roxburghii Rroxscaffold_3G00267820
rosa_rugosa Rorug06G0476700
rosa_samantha Rh7AG063800 Rh7BG080300 Rh7CG083700 Rh7DG084600
rosa_wichuraiana Rw7G007260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 90
AccII CGCG 1 cut(s) 225
AclWI GGATC 3 cut(s) 512, 590, 746
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 580, 662
AcuI CTGAAG 1 cut(s) 398
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 2 cut(s) 14, 238
AgeI ACCGGT 1 cut(s) 444
AgsI TTSAA 8 cut(s) 564, 580, 635, 778, 812, 818, 893, 1081
AjnI CCWGG 2 cut(s) 231, 994
AleI CACNNNNGTG 1 cut(s) 1017
AluBI AGCT 7 cut(s) 144, 355, 412, 471, 707, 799, 973
AluI AGCT 7 cut(s) 144, 355, 412, 471, 707, 799, 973
Alw21I GWGCWC 3 cut(s) 444, 975, 1057
Alw26I GTCTC 2 cut(s) 734, 977
Alw44I GTGCAC 1 cut(s) 440
AlwI GGATC 3 cut(s) 512, 590, 746
AoxI GGCC 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 440
ApeKI GCWGC 3 cut(s) 141, 168, 657
ApoI RAATTY 2 cut(s) 580, 662
AsiGI ACCGGT 1 cut(s) 444
Asp700I GAANNNNTTC 2 cut(s) 317, 630
AspS9I GGNCC 3 cut(s) 129, 292, 523
AsuHPI GGTGA 4 cut(s) 105, 299, 422, 773
AvaII GGWCC 3 cut(s) 129, 292, 523
BaeGI GKGCMC 2 cut(s) 339, 444
BanII GRGCYC 1 cut(s) 975
BbsI GAAGAC 2 cut(s) 40, 552
Bbv12I GWGCWC 3 cut(s) 444, 975, 1057
BbvI GCAGC 3 cut(s) 153, 155, 669
BccI CCATC 3 cut(s) 715, 735, 851
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 2 cut(s) 233, 996
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 2 cut(s) 734, 977
BfaI CTAG 2 cut(s) 11, 285
BfuAI ACCTGC 1 cut(s) 564
BisI GCNGC 6 cut(s) 102, 105, 142, 169, 658, 839
BlpI GCTNAGC 1 cut(s) 356
BlsI GCNGC 6 cut(s) 103, 106, 143, 170, 659, 840
Bme1390I CCNGG 2 cut(s) 233, 996
Bme18I GGWCC 3 cut(s) 129, 292, 523
BmgT120I GGNCC 3 cut(s) 129, 292, 523
BmiI GGNNCC 3 cut(s) 130, 131, 293
BmrFI CCNGG 2 cut(s) 233, 996
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 3 cut(s) 241, 478, 950
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 40, 552
BplI GAGNNNNNCTC 2 cut(s) 959, 991
BpmI CTGGAG 1 cut(s) 904
Bpu10I CCTNAGC 2 cut(s) 117, 959
Bpu1102I GCTNAGC 1 cut(s) 356
Bsa29I ATCGAT 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 163, 231, 791, 1062
BsaWI WCCGGW 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 238
Bse118I RCCGGY 1 cut(s) 444
Bse1I ACTGG 2 cut(s) 887, 991
Bse3DI GCAATG 1 cut(s) 648
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 2 cut(s) 233, 996
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 4 cut(s) 163, 231, 791, 1062
BseGI GGATG 3 cut(s) 256, 871, 1004
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 2 cut(s) 14, 238
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 887, 991
BseRI GAGGAG 2 cut(s) 338, 983
BseSI GKGCMC 2 cut(s) 339, 444
BseXI GCAGC 3 cut(s) 153, 155, 669
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 444
BshVI ATCGAT 1 cut(s) 781
BsiHKAI GWGCWC 3 cut(s) 444, 975, 1057
BsiSI CCGG 1 cut(s) 445
BslFI GGGAC 3 cut(s) 142, 305, 977
BslI CCNNNNNNNGG 2 cut(s) 14, 238
BsmAI GTCTC 2 cut(s) 734, 977
BsmFI GGGAC 3 cut(s) 142, 305, 977
BsnI GGCC 1 cut(s) 101
Bsp1286I GDGCHC 4 cut(s) 339, 444, 975, 1057
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 6 cut(s) 301, 504, 595, 601, 710, 751
Bsp1720I GCTNAGC 1 cut(s) 356
Bsp19I CCATGG 1 cut(s) 1062
Bsp68I TCGCGA 1 cut(s) 225
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 348
BspDI ATCGAT 1 cut(s) 781
BspFNI CGCG 1 cut(s) 225
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 3 cut(s) 130, 131, 293
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 3 cut(s) 512, 590, 746
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 2 cut(s) 887, 991
BssAI RCCGGY 1 cut(s) 444
BssECI CCNNGG 4 cut(s) 163, 231, 791, 1062
BssMI GATC 6 cut(s) 301, 504, 595, 601, 710, 751
BssT1I CCWWGG 2 cut(s) 791, 1062
Bst2UI CCWGG 2 cut(s) 233, 996
Bst6I CTCTTC 1 cut(s) 813
BstAPI GCANNNNNTGC 1 cut(s) 488
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 655
BstDEI CTNAG 3 cut(s) 117, 356, 959
BstDSI CCRYGG 1 cut(s) 1062
BstEII GGTNACC 2 cut(s) 428, 699
BstF5I GGATG 3 cut(s) 256, 871, 1004
BstFNI CGCG 1 cut(s) 225
BstKTI GATC 6 cut(s) 304, 507, 598, 604, 713, 754
BstMAI GTCTC 2 cut(s) 734, 977
BstMBI GATC 6 cut(s) 301, 504, 595, 601, 710, 751
BstMWI GCNNNNNNNGC 2 cut(s) 150, 488
BstNI CCWGG 2 cut(s) 233, 996
BstPI GGTNACC 2 cut(s) 428, 699
BstSCI CCNGG 2 cut(s) 231, 994
BstSLI GKGCMC 2 cut(s) 339, 444
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 153, 155, 669
BstV2I GAAGAC 2 cut(s) 40, 552
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 781
BsuRI GGCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 781
BtgI CCRYGG 1 cut(s) 1062
BtsCI GGATG 3 cut(s) 256, 871, 1004
BtsI GCAGTG 2 cut(s) 148, 367
BtsIMutI CAGTG 3 cut(s) 148, 367, 880
BtuMI TCGCGA 1 cut(s) 225
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 655
CciI TCATGA 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 444
Cfr13I GGNCC 3 cut(s) 129, 292, 523
ClaI ATCGAT 1 cut(s) 781
Csp6I GTAC 1 cut(s) 463
CspAI ACCGGT 1 cut(s) 444
CviAII CATG 7 cut(s) 97, 175, 686, 696, 769, 1015, 1063
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 3 cut(s) 117, 356, 959
DpnI GATC 6 cut(s) 303, 506, 597, 603, 712, 753
DpnII GATC 6 cut(s) 301, 504, 595, 601, 710, 751
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 813
EarI CTCTTC 1 cut(s) 813
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 2 cut(s) 791, 1062
Eco24I GRGCYC 1 cut(s) 975
Eco47I GGWCC 3 cut(s) 129, 292, 523
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 398
Eco91I GGTNACC 2 cut(s) 428, 699
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 1 cut(s) 129
EcoO65I GGTNACC 2 cut(s) 428, 699
EcoRII CCWGG 2 cut(s) 231, 994
EcoT14I CCWWGG 2 cut(s) 791, 1062
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 2 cut(s) 791, 1062
FaeI CATG 7 cut(s) 100, 178, 689, 699, 772, 1018, 1066
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 3 cut(s) 142, 305, 977
FatI CATG 7 cut(s) 96, 174, 685, 695, 768, 1014, 1062
FauI CCCGC 4 cut(s) 61, 95, 646, 945
FbaI TGATCA 1 cut(s) 601
Fnu4HI GCNGC 6 cut(s) 102, 105, 142, 169, 658, 839
FokI GGATG 3 cut(s) 263, 878, 1011
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 6 cut(s) 102, 105, 142, 169, 658, 839
FspBI CTAG 2 cut(s) 11, 285
GluI GCNGC 6 cut(s) 102, 105, 142, 169, 658, 839
GsuI CTGGAG 1 cut(s) 904
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 445
Hin1II CATG 7 cut(s) 100, 178, 689, 699, 772, 1018, 1066
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 2 cut(s) 469, 797
HinfI GANTC 4 cut(s) 178, 281, 778, 991
HpaII CCGG 1 cut(s) 445
HphI GGTGA 4 cut(s) 105, 299, 422, 773
Hpy166II GTNNAC 5 cut(s) 376, 442, 463, 496, 1012
Hpy188I TCNGA 2 cut(s) 613, 1087
Hpy188III TCNNGA 9 cut(s) 175, 224, 248, 290, 388, 599, 718, 863, 911
Hpy8I GTNNAC 5 cut(s) 376, 442, 463, 496, 1012
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 5 cut(s) 307, 385, 400, 419, 1043
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 4 cut(s) 442, 689, 902, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 150, 488
HpyF3I CTNAG 3 cut(s) 117, 356, 959
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 7 cut(s) 100, 178, 689, 699, 772, 1018, 1066
KflI GGGWCCC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 6 cut(s) 301, 504, 595, 601, 710, 751
LmnI GCTCC 2 cut(s) 409, 970
Lsp1109I GCAGC 3 cut(s) 153, 155, 669
LweI GCATC 3 cut(s) 241, 478, 950
MaeI CTAG 2 cut(s) 11, 285
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 428, 699, 904
MalI GATC 6 cut(s) 303, 506, 597, 603, 712, 753
MbiI CCGCTC 1 cut(s) 90
MboI GATC 6 cut(s) 301, 504, 595, 601, 710, 751
MboII GAAGA 4 cut(s) 40, 552, 830, 841
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 4 cut(s) 339, 444, 975, 1057
MluCI AATT 3 cut(s) 483, 580, 662
MmeI TCCRAC 1 cut(s) 225
MroXI GAANNNNTTC 2 cut(s) 317, 630
MseI TTAA 3 cut(s) 27, 345, 668
MslI CAYNNNNRTG 3 cut(s) 435, 946, 1017
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 2 cut(s) 233, 996
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 2 cut(s) 233, 996
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 150, 488
NcoI CCATGG 1 cut(s) 1062
NdeII GATC 6 cut(s) 301, 504, 595, 601, 710, 751
NlaIII CATG 7 cut(s) 100, 178, 689, 699, 772, 1018, 1066
NlaIV GGNNCC 3 cut(s) 130, 131, 293
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 2 cut(s) 428, 904
NruI TCGCGA 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 1017
PagI TCATGA 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 2 cut(s) 317, 630
PfeI GAWTC 4 cut(s) 178, 281, 778, 991
PflMI CCANNNNNTGG 1 cut(s) 238
PfoI TCCNGGA 1 cut(s) 994
PinAI ACCGGT 1 cut(s) 444
PkrI GCNGC 6 cut(s) 103, 106, 143, 170, 659, 840
PpuMI RGGWCCY 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 231, 994
PspEI GGTNACC 2 cut(s) 428, 699
PspGI CCWGG 2 cut(s) 231, 994
PspN4I GGNNCC 3 cut(s) 130, 131, 293
PspPI GGNCC 3 cut(s) 129, 292, 523
PspPPI RGGWCCY 1 cut(s) 129
PsuI RGATCY 1 cut(s) 595
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 3 cut(s) 435, 946, 1017
SacI GAGCTC 1 cut(s) 975
SaqAI TTAA 3 cut(s) 27, 345, 668
SatI GCNGC 6 cut(s) 102, 105, 142, 169, 658, 839
Sau3AI GATC 6 cut(s) 301, 504, 595, 601, 710, 751
Sau96I GGNCC 3 cut(s) 129, 292, 523
ScrFI CCNGG 2 cut(s) 233, 996
SduI GDGCHC 4 cut(s) 339, 444, 975, 1057
SfaNI GCATC 3 cut(s) 241, 478, 950
SinI GGWCC 3 cut(s) 129, 292, 523
SmiMI CAYNNNNRTG 3 cut(s) 435, 946, 1017
Sse9I AATT 3 cut(s) 483, 580, 662
SspMI CTAG 2 cut(s) 11, 285
SstI GAGCTC 1 cut(s) 975
StyD4I CCNGG 2 cut(s) 231, 994
StyI CCWWGG 2 cut(s) 791, 1062
TaiI ACGT 1 cut(s) 267
TaqI TCGA 5 cut(s) 321, 420, 435, 709, 781
TaqII GACCGA 1 cut(s) 309
TasI AATT 3 cut(s) 483, 580, 662
TatI WGTACW 1 cut(s) 462
TauI GCSGC 3 cut(s) 104, 107, 841
TfiI GAWTC 4 cut(s) 178, 281, 778, 991
Tru1I TTAA 3 cut(s) 27, 345, 668
Tru9I TTAA 3 cut(s) 27, 345, 668
TscAI CASTG 3 cut(s) 155, 367, 887
TseFI GTSAC 2 cut(s) 428, 904
TseI GCWGC 3 cut(s) 141, 168, 657
Tsp45I GTSAC 2 cut(s) 428, 904
TspDTI ATGAA 6 cut(s) 163, 438, 674, 791, 842, 1062
TspGWI ACGGA 2 cut(s) 536, 668
TspRI CASTG 3 cut(s) 155, 367, 887
Van91I CCANNNNNTGG 1 cut(s) 238
VneI GTGCAC 1 cut(s) 440
VpaK11BI GGWCC 3 cut(s) 129, 292, 523
XapI RAATTY 2 cut(s) 580, 662
XmnI GAANNNNTTC 2 cut(s) 317, 630
XspI CTAG 2 cut(s) 11, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.