AT3G50450

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014069
Physical Location & Seq
Forward (+)
18722234 .. 18723310
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50450.1

Sequence Viewer

Length: 570 bp
ATGCCTCTTGTTGAGCTTCTTACAAGTGCTGCTCTTGGATTGTCCCTCCAACTCCTTCACGACGCCATCATAAGAGCCAAGGAGAGATCCTTAATCACAAGATGTATATTGGACCGTCTCGATGCTACACTCCATAAGATAACTCCATTTGTCATTAAGATCGATACCCTCACCGAAGAAGTCGATGAACCTTTCAGAAAAGTCATCGAAGAGCTTAAACGTCTTCTCGAGAAAGCCATTCGTCTCGTTGATGCTTATGCGGAGCTTAAACTCCGAAACTTACTCAGGAAATATAGGTACAAGAGAAGAATCAAGGAGTTGGACTCTTCTTTGAGATGGATGATAGATGTGGATGTTCAAGTCAATCAATGGCTGGATATCAAAAAACTTATGGGTAAGATGTCTGAAATGAACACTAAACTCGATGATATTACGCGTCAACCAATGGATATCATCGAAGCAACTGGTCGATCTTCTGAAGAAGATGGATGCACAAAGCCAACGATTGATATACACTTTCGATGGAAAAACCAGACCAAAGAGCACGAGATCCGGTTCATCTTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

189

Amino Acids

22.34

Weight (kDa)

9.02

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 142 1.2e-52 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 436
AciI CCGC 1 cut(s) 260
AclWI GGATC 2 cut(s) 81, 544
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 434
AgsI TTSAA 2 cut(s) 359, 565
AluBI AGCT 3 cut(s) 16, 214, 265
AluI AGCT 3 cut(s) 16, 214, 265
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 122, 248
AlwI GGATC 2 cut(s) 81, 544
Ama87I CYCGRG 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 112
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 1 cut(s) 16
BccI CCATC 4 cut(s) 74, 330, 479, 516
BcoDI GTCTC 2 cut(s) 122, 248
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 112
BmsI GCATC 3 cut(s) 112, 241, 479
BpiI GAAGAC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 308, 340
Bsa29I ATCGAT 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 552
Bse1I ACTGG 1 cut(s) 469
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 3 cut(s) 345, 358, 494
BseMII CTCAG 1 cut(s) 298
BseNI ACTGG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 436
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 227
BsiSI CCGG 1 cut(s) 553
BslFI GGGAC 1 cut(s) 28
BsmAI GTCTC 2 cut(s) 122, 248
BsmBI CGTCTC 2 cut(s) 122, 248
BsmFI GGGAC 1 cut(s) 28
BsoBI CYCGRG 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 546
Bsp143I GATC 4 cut(s) 86, 159, 470, 549
BspACI CCGC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 297
BspDI ATCGAT 1 cut(s) 162
BspFNI CGCG 1 cut(s) 436
BspPI GGATC 2 cut(s) 81, 544
BspQI GCTCTTC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 4 cut(s) 86, 159, 470, 549
BssNI GRCGYC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 545
BssT1I CCWWGG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 545
Bst4CI ACNGT 1 cut(s) 116
Bst6I CTCTTC 2 cut(s) 204, 331
BstACI GRCGYC 1 cut(s) 63
BstDEI CTNAG 1 cut(s) 284
BstF5I GGATG 3 cut(s) 345, 358, 494
BstFNI CGCG 1 cut(s) 436
BstKTI GATC 4 cut(s) 89, 162, 473, 552
BstMAI GTCTC 2 cut(s) 122, 248
BstMBI GATC 4 cut(s) 86, 159, 470, 549
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 86, 549
BstYI RGATCY 2 cut(s) 86, 549
Bsu15I ATCGAT 1 cut(s) 162
BsuTUI ATCGAT 1 cut(s) 162
BtsCI GGATG 3 cut(s) 345, 358, 494
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 162
CseI GACGC 2 cut(s) 71, 425
Csp6I GTAC 1 cut(s) 298
CviJI RGCY 7 cut(s) 16, 77, 214, 236, 265, 373, 499
CviKI_1 RGCY 7 cut(s) 16, 77, 214, 236, 265, 373, 499
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 284
DpnI GATC 4 cut(s) 88, 161, 472, 551
DpnII GATC 4 cut(s) 86, 159, 470, 549
Eam1104I CTCTTC 2 cut(s) 204, 331
EarI CTCTTC 2 cut(s) 204, 331
Eco130I CCWWGG 1 cut(s) 78
Eco32I GATATC 2 cut(s) 379, 451
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 227
EcoRV GATATC 2 cut(s) 379, 451
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 2 cut(s) 122, 248
FaiI YATR 7 cut(s) 71, 107, 135, 258, 294, 392, 512
FaqI GGGAC 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 3 cut(s) 352, 365, 501
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HapII CCGG 1 cut(s) 553
HgaI GACGC 2 cut(s) 71, 425
Hin1I GRCGYC 1 cut(s) 63
HincII GTYRAC 1 cut(s) 440
HindII GTYRAC 1 cut(s) 440
HinfI GANTC 2 cut(s) 309, 323
HpaII CCGG 1 cut(s) 553
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 4 cut(s) 197, 275, 406, 478
Hpy188III TCNNGA 5 cut(s) 59, 119, 227, 229, 286
Hpy8I GTNNAC 1 cut(s) 440
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 1 cut(s) 492
HpyF3I CTNAG 1 cut(s) 284
HpySE526I ACGT 1 cut(s) 220
Hsp92I GRCGYC 1 cut(s) 63
Kzo9I GATC 4 cut(s) 86, 159, 470, 549
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 5 cut(s) 271, 359, 450, 545, 566
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 112, 241, 479
MaeII ACGT 1 cut(s) 220
MalI GATC 4 cut(s) 88, 161, 472, 551
MboI GATC 4 cut(s) 86, 159, 470, 549
MboII GAAGA 9 cut(s) 188, 215, 221, 318, 318, 465, 491, 494, 553
MflI RGATCY 2 cut(s) 86, 549
MhlI GDGCHC 1 cut(s) 546
MluI ACGCGT 1 cut(s) 434
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 73, 300
MnlI CCTC 3 cut(s) 15, 56, 179
MseI TTAA 4 cut(s) 92, 156, 216, 267
MspI CCGG 1 cut(s) 553
MvnI CGCG 1 cut(s) 436
NdeII GATC 4 cut(s) 86, 159, 470, 549
PaeR7I CTCGAG 1 cut(s) 227
PciSI GCTCTTC 1 cut(s) 204
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 2 cut(s) 86, 549
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 4 cut(s) 92, 156, 216, 267
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 4 cut(s) 86, 159, 470, 549
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 1 cut(s) 546
SetI ASST 6 cut(s) 18, 193, 216, 223, 267, 299
SfaNI GCATC 3 cut(s) 112, 241, 479
Sfr274I CTCGAG 1 cut(s) 227
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SsiI CCGC 1 cut(s) 260
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 223
TaqI TCGA 9 cut(s) 120, 162, 183, 207, 228, 423, 456, 469, 520
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 4 cut(s) 92, 156, 216, 267
Tru9I TTAA 4 cut(s) 92, 156, 216, 267
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 201, 425, 547
VpaK11BI GGWCC 1 cut(s) 112
XhoI CTCGAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.