AT3G50480

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014069
Physical Location & Seq
Forward (+)
18733021 .. 18734420
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50480.1

Sequence Viewer

Length: 603 bp
ATGCCGATTGCTGAGCTTGCTGTAATCAAAACAGTGGGTGGGCCTCTGATAGCGGCTGCTCTTGGAGTTGGAGCCCAAGTCATTTACGACGGCTTCAGAAAAGGAAAAGATACATCTATCATAAACCGTCTCGGCCGTACAATGGAAAGTATCAGTCCGGTGCGGGATCGAATCGGTAAGCTCAGTAATGTGGAAGGTAAACCATTTAGGGAAGTCCACGAAAGTCTTACGCGTCTCCTTGAAGATGCGAAATCTATTATTGAGAAATATTGGAAACTCAGATGGAGCAGACACGTTTGCAGGAAGTACAGGTACATCAAAAAACTCGAATCGATAGAACTTGAATTAGTAAGGGTAGCACGTGAGATACAAGTCCACCAATGGACCGATATCAAAGAAATGAAGGCGATACAAGTCCACCAATGGACCGATATCAAAGAAATGAAGGCGATACAAGTCGACCAATGGACCGATATCAAAGAAATGAAGGCGATACAAGTCGACCAATGGATCGATATCAAAGAAATGAAGGCGATACAAGTCGACCAATGGACCGATATCAAAGAAATGAAGGCCCAGATATCTGAAAAGCATAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

200

Amino Acids

23.39

Weight (kDa)

9.38

Isoelectric Point (pI)

18.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 147 1.6e-42 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 459, 501, 543
AccII CGCG 1 cut(s) 232
AciI CCGC 2 cut(s) 53, 163
AclWI GGATC 2 cut(s) 174, 518
AcoI YGGCCR 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 79
AcvI CACGTG 1 cut(s) 362
AfaI GTAC 3 cut(s) 139, 308, 314
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 2 cut(s) 230, 292
AgsI TTSAA 2 cut(s) 242, 344
AluBI AGCT 2 cut(s) 16, 181
AluI AGCT 2 cut(s) 16, 181
Alw26I GTCTC 2 cut(s) 134, 239
AlwI GGATC 2 cut(s) 174, 518
AoxI GGCC 3 cut(s) 41, 133, 573
ApeKI GCWGC 1 cut(s) 56
AspS9I GGNCC 6 cut(s) 41, 384, 426, 468, 552, 574
AvaII GGWCC 4 cut(s) 384, 426, 468, 552
BanII GRGCYC 1 cut(s) 76
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbrPI CACGTG 1 cut(s) 362
BbvI GCAGC 1 cut(s) 43
BccI CCATC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 106, 120
BcoDI GTCTC 2 cut(s) 134, 239
BisI GCNGC 2 cut(s) 54, 57
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 2 cut(s) 55, 58
Bme18I GGWCC 4 cut(s) 384, 426, 468, 552
BmgT120I GGNCC 6 cut(s) 41, 384, 426, 468, 552, 574
BmiI GGNNCC 1 cut(s) 73
BmsI GCATC 1 cut(s) 235
Bpu1102I GCTNAGC 1 cut(s) 12
Bsa29I ATCGAT 2 cut(s) 332, 513
BsaAI YACGTR 1 cut(s) 362
BsaBI GATNNNNATC 1 cut(s) 515
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse8I GATNNNNATC 1 cut(s) 515
BseCI ATCGAT 2 cut(s) 332, 513
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 196, 292
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 232
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 3 cut(s) 43, 135, 575
BshVI ATCGAT 2 cut(s) 332, 513
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 134, 239
BsmBI CGTCTC 2 cut(s) 134, 239
BsnI GGCC 3 cut(s) 43, 135, 575
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 166, 510
Bsp1720I GCTNAGC 1 cut(s) 12
BspACI CCGC 2 cut(s) 53, 163
BspANI GGCC 3 cut(s) 43, 135, 575
BspCNI CTCAG 3 cut(s) 4, 195, 291
BspDI ATCGAT 2 cut(s) 332, 513
BspFNI CGCG 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 73
BspPI GGATC 2 cut(s) 174, 518
BssMI GATC 2 cut(s) 166, 510
Bst4CI ACNGT 2 cut(s) 34, 128
BstBAI YACGTR 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 3 cut(s) 12, 182, 278
BstFNI CGCG 1 cut(s) 232
BstKTI GATC 2 cut(s) 169, 513
BstMAI GTCTC 2 cut(s) 134, 239
BstMBI GATC 2 cut(s) 166, 510
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstUI CGCG 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 43
BstZI CGGCCG 1 cut(s) 133
Bsu15I ATCGAT 2 cut(s) 332, 513
BsuRI GGCC 3 cut(s) 43, 135, 575
BsuTUI ATCGAT 2 cut(s) 332, 513
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 18
Cfr13I GGNCC 6 cut(s) 41, 384, 426, 468, 552, 574
ClaI ATCGAT 2 cut(s) 332, 513
CseI GACGC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 138, 307, 313
CviJI RGCY 8 cut(s) 16, 43, 56, 74, 93, 135, 181, 575
CviKI_1 RGCY 8 cut(s) 16, 43, 56, 74, 93, 135, 181, 575
CviQI GTAC 3 cut(s) 138, 307, 313
DdeI CTNAG 3 cut(s) 12, 182, 278
DpnI GATC 2 cut(s) 168, 512
DpnII GATC 2 cut(s) 166, 510
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
EclXI CGGCCG 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 76
Eco32I GATATC 6 cut(s) 391, 433, 475, 517, 559, 582
Eco47I GGWCC 4 cut(s) 384, 426, 468, 552
Eco52I CGGCCG 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 79
Eco72I CACGTG 1 cut(s) 362
EcoRV GATATC 6 cut(s) 391, 433, 475, 517, 559, 582
EcoT38I GRGCYC 1 cut(s) 76
Esp3I CGTCTC 2 cut(s) 134, 239
FaiI YATR 2 cut(s) 122, 594
FauI CCCGC 1 cut(s) 156
FblI GTMKAC 3 cut(s) 459, 501, 543
Fnu4HI GCNGC 2 cut(s) 54, 57
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 2 cut(s) 54, 57
GluI GCNGC 2 cut(s) 54, 57
HaeIII GGCC 3 cut(s) 43, 135, 575
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 221
HincII GTYRAC 3 cut(s) 460, 502, 544
HindII GTYRAC 3 cut(s) 460, 502, 544
HinfI GANTC 2 cut(s) 171, 329
HpaII CCGG 1 cut(s) 158
Hpy166II GTNNAC 7 cut(s) 200, 217, 376, 418, 460, 502, 544
Hpy188I TCNGA 4 cut(s) 48, 98, 281, 586
Hpy8I GTNNAC 7 cut(s) 200, 217, 376, 418, 460, 502, 544
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 6 cut(s) 188, 397, 439, 481, 523, 565
HpyCH4III ACNGT 2 cut(s) 34, 128
HpyCH4IV ACGT 2 cut(s) 294, 361
HpyCH4V TGCA 1 cut(s) 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 3 cut(s) 12, 182, 278
HpySE526I ACGT 2 cut(s) 294, 361
Kzo9I GATC 2 cut(s) 166, 510
LmnI GCTCC 2 cut(s) 71, 285
LpnPI CCDG 4 cut(s) 171, 286, 295, 590
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 1 cut(s) 235
MaeII ACGT 2 cut(s) 294, 361
MalI GATC 2 cut(s) 168, 512
MboI GATC 2 cut(s) 166, 510
MboII GAAGA 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 1 cut(s) 344
MluI ACGCGT 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 1 cut(s) 54
MspI CCGG 1 cut(s) 158
MvnI CGCG 1 cut(s) 232
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 2 cut(s) 166, 510
NlaIV GGNNCC 1 cut(s) 73
NmeAIII GCCGAG 1 cut(s) 111
PfeI GAWTC 2 cut(s) 171, 329
PkrI GCNGC 2 cut(s) 55, 58
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 362
PspCI CACGTG 1 cut(s) 362
PspN4I GGNNCC 1 cut(s) 73
PspPI GGNCC 6 cut(s) 41, 384, 426, 468, 552, 574
RsaI GTAC 3 cut(s) 139, 308, 314
RsaNI GTAC 3 cut(s) 138, 307, 313
SalI GTCGAC 3 cut(s) 458, 500, 542
SatI GCNGC 2 cut(s) 54, 57
Sau3AI GATC 2 cut(s) 166, 510
Sau96I GGNCC 6 cut(s) 41, 384, 426, 468, 552, 574
SduI GDGCHC 1 cut(s) 76
SetI ASST 6 cut(s) 18, 183, 199, 297, 314, 364
SfaNI GCATC 1 cut(s) 235
SinI GGWCC 4 cut(s) 384, 426, 468, 552
Sse9I AATT 1 cut(s) 344
SsiI CCGC 2 cut(s) 53, 163
SspI AATATT 1 cut(s) 269
TaaI ACNGT 2 cut(s) 34, 128
TaiI ACGT 2 cut(s) 297, 364
TaqI TCGA 7 cut(s) 169, 327, 332, 459, 501, 513, 543
TaqII GACCGA 4 cut(s) 401, 443, 485, 569
TasI AATT 1 cut(s) 344
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 171, 329
TscAI CASTG 1 cut(s) 39
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 5 cut(s) 416, 458, 500, 542, 584
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 4 cut(s) 384, 426, 468, 552
XmiI GTMKAC 3 cut(s) 459, 501, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.