Rh6DG281400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0014069
Physical Location & Seq
Reverse (-)
47559923 .. 47563468
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG281400.1

Sequence Viewer

Length: 576 bp
ATGGCTGGAATTGGAGAGCTTGCTGCAGGGGGTGCAGTAGGACTAGCATTTTCTTTGCTGTATGAAGGCGTTAGCACACTTGTCATCAAATCTAAGGAGTTTAAACCCCTTCTCGAAGAGCTCATAGTCACGTTAGACTACTTAAAACCACTGATCAAACAGATAGGAGCTGGTAACAGGGAACTGGATCACCCAAATGAAAAGGTATATTTTGAGAACCAAATGGAGAAGGGCAAGAGGCTCATTGACAAGTTGTCCACAGTTGGTAGTTGGAATTTGCTCAAGCCTTATTACAGCAATAAGCTTGTTGAATTTGATAGGTCTCTCAGCAAGATGTTGCTGAATCTAATACCGCAGGGAGTAAGGGATGGGCAAGATATATTGGTTATGTTATTTCTTTCTTTTCTGGTGAGATTTGTATGGCAAGAGCTAGAAAATTCAACTCTCGATGAGATAAGGAATGAGCTTTCAACAGTTCAGTACATGCACACCACAAATGAAATTAGTAATTTTCTCTTCACTACTTTCAGTGATAGAATTAGGGAGGGAATAGTTTCTCTTCATGCTAGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

5.46

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 130 6.7e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 353
AclWI GGATC 1 cut(s) 195
AcsI RAATTY 3 cut(s) 274, 311, 436
AfaI GTAC 1 cut(s) 482
AgsI TTSAA 3 cut(s) 311, 441, 471
AhdI GACNNNNNGTC 1 cut(s) 253
AluBI AGCT 6 cut(s) 19, 121, 170, 304, 430, 466
AluI AGCT 6 cut(s) 19, 121, 170, 304, 430, 466
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 1 cut(s) 195
ApeKI GCWGC 1 cut(s) 23
ApoI RAATTY 3 cut(s) 274, 311, 436
Asp700I GAANNNNTTC 1 cut(s) 553
AsuHPI GGTGA 2 cut(s) 182, 421
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 10
BccI CCATC 1 cut(s) 362
BclI TGATCA 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 3 cut(s) 44, 431, 567
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
BmeRI GACNNNNNGTC 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 340
BseNI ACTGG 1 cut(s) 189
BseXI GCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 2 cut(s) 153, 187
BspACI CCGC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 339
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 1 cut(s) 195
BspQI GCTCTTC 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 189
BssMI GATC 2 cut(s) 153, 187
Bst4CI ACNGT 2 cut(s) 262, 475
Bst6I CTCTTC 3 cut(s) 111, 521, 564
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 93, 326
BstF5I GGATG 1 cut(s) 373
BstKTI GATC 2 cut(s) 156, 190
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 2 cut(s) 153, 187
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 10
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 2 cut(s) 149, 535
Cac8I GCNNGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 484, 563
CviJI RGCY 9 cut(s) 5, 19, 121, 170, 241, 286, 304, 430, 466
CviKI_1 RGCY 9 cut(s) 5, 19, 121, 170, 241, 286, 304, 430, 466
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 2 cut(s) 93, 326
DpnI GATC 2 cut(s) 155, 189
DpnII GATC 2 cut(s) 153, 187
DraI TTTAAA 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 111, 521, 564
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 3 cut(s) 111, 521, 564
Ecl136II GAGCTC 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 123
FaeI CATG 2 cut(s) 487, 566
FaiI YATR 9 cut(s) 63, 125, 208, 380, 389, 421, 485, 564, 574
FatI CATG 2 cut(s) 483, 562
FbaI TGATCA 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 24
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 3 cut(s) 44, 431, 567
GluI GCNGC 1 cut(s) 24
Hin1II CATG 2 cut(s) 487, 566
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 1 cut(s) 343
HphI GGTGA 2 cut(s) 182, 421
Hpy166II GTNNAC 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 113, 446
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 3 cut(s) 59, 119, 223
HpyCH4III ACNGT 2 cut(s) 262, 475
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 3 cut(s) 26, 35, 487
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 2 cut(s) 93, 326
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 2 cut(s) 487, 566
Ksp22I TGATCA 1 cut(s) 153
Kzo9I GATC 2 cut(s) 153, 187
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 6 cut(s) 12, 156, 163, 170, 341, 392
Lsp1109I GCAGC 1 cut(s) 10
MaeI CTAG 3 cut(s) 44, 431, 567
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 127, 173
MalI GATC 2 cut(s) 155, 189
MboI GATC 2 cut(s) 153, 187
MboII GAAGA 3 cut(s) 128, 508, 551
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 7 cut(s) 9, 274, 311, 436, 501, 508, 537
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 2 cut(s) 231, 538
MroXI GAANNNNTTC 1 cut(s) 553
MseI TTAA 2 cut(s) 102, 143
MslI CAYNNNNRTG 1 cut(s) 195
MssI GTTTAAAC 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeII GATC 2 cut(s) 153, 187
NlaIII CATG 2 cut(s) 487, 566
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 553
PfeI GAWTC 1 cut(s) 343
PkrI GCNGC 1 cut(s) 25
PmeI GTTTAAAC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 123
PstI CTGCAG 1 cut(s) 28
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 123
SapI GCTCTTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 102, 143
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 2 cut(s) 153, 187
SduI GDGCHC 1 cut(s) 123
SetI ASST 9 cut(s) 21, 123, 134, 172, 207, 306, 323, 432, 468
SfcI CTRYAG 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 7 cut(s) 9, 274, 311, 436, 501, 508, 537
SsiI CCGC 1 cut(s) 353
SspMI CTAG 3 cut(s) 44, 431, 567
SstI GAGCTC 1 cut(s) 123
TaaI ACNGT 2 cut(s) 262, 475
TaiI ACGT 1 cut(s) 134
TaqI TCGA 2 cut(s) 114, 447
TasI AATT 7 cut(s) 9, 274, 311, 436, 501, 508, 537
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 2 cut(s) 102, 143
Tru9I TTAA 2 cut(s) 102, 143
TscAI CASTG 2 cut(s) 156, 535
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 1 cut(s) 23
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 5 cut(s) 78, 213, 513, 551, 561
TspRI CASTG 2 cut(s) 156, 535
XapI RAATTY 3 cut(s) 274, 311, 436
XceI RCATGY 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 553
XspI CTAG 3 cut(s) 44, 431, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.