Rw2G013210

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014069
Physical Location & Seq
Forward (+)
13952109 .. 13952687
579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013210.1

Sequence Viewer

Length: 543 bp
ATGGAACAACTCTTTATAGAGGCTACAATAGGACATGCACTAGAGGCCTTATTGAGAGCTGTTTCACAAGCCAGGGACAGAGCGCACAACTATGAGTCCATTCTTGCACAACTTGAATTCACAATCCAATCCATCGCTCCAAAAATCTATGAAATCGATTGTTTATCTGAAGAACATCTCCAGGATGATGTGGAGTTAGTCGATAAGTGTTCAAAGTCGGGTTGCTGCACCTTCTACAACAGCCAGAGGTACTACAAGAAGCTCCAAGAGTTGGATATTTCTCTTGGAAGGCTGATTACGATTGAGTTGCAAGTTAAGGTGGCATTGGATGTTGTAAGGATTTCGGCTGACATGAAGGAGCTCTGTAAGAGATGGGATGATCAAGTGAAGAATGTACATGAAGAGGGAGGACAGAGTACTTGGATTTTCAAGCTAAGTGGAAGAAAGCTTTTGGGTAGGTTTGCTGGACTAAAGAAGATTGTATTGTACAAACCAAAGAGAATCCTTCTGAATTTGGTTACTTGTGGAGCTATGGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050896 GO:0051704 GO:0051707
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

180

Amino Acids

20.61

Weight (kDa)

6.44

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 122 3.8e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 271
AcsI RAATTY 2 cut(s) 116, 511
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 4 cut(s) 251, 396, 418, 488
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 3 cut(s) 116, 213, 430
AjnI CCWGG 2 cut(s) 71, 180
AluBI AGCT 6 cut(s) 59, 262, 361, 433, 448, 530
AluI AGCT 6 cut(s) 59, 262, 361, 433, 448, 530
Alw21I GWGCWC 1 cut(s) 363
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 225
ApoI RAATTY 2 cut(s) 116, 511
AspLEI GCGC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 280, 312
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 212
BccI CCATC 2 cut(s) 140, 366
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 2 cut(s) 73, 182
BclI TGATCA 1 cut(s) 379
BfaI CTAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
BmcAI AGTACT 1 cut(s) 418
Bme1390I CCNGG 2 cut(s) 73, 182
BmrFI CCNGG 2 cut(s) 73, 182
BpmI CTGGAG 1 cut(s) 164
Bsa29I ATCGAT 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 72
Bsc4I CCNNNNNNNGG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 73, 182
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 3 cut(s) 190, 334, 382
BseLI CCNNNNNNNGG 1 cut(s) 271
BseXI GCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 211
BshFI GGCC 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 363
Bsp1407I TGTACA 2 cut(s) 394, 486
Bsp143I GATC 1 cut(s) 379
BspANI GGCC 1 cut(s) 47
BspDI ATCGAT 1 cut(s) 156
BsrGI TGTACA 2 cut(s) 394, 486
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 379
Bst2UI CCWGG 2 cut(s) 73, 182
Bst6I CTCTTC 1 cut(s) 396
BstAUI TGTACA 2 cut(s) 394, 486
BstDEI CTNAG 1 cut(s) 434
BstF5I GGATG 3 cut(s) 190, 334, 382
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 382
BstMBI GATC 1 cut(s) 379
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 2 cut(s) 73, 182
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 71, 180
BstV1I GCAGC 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 156
BsuRI GGCC 1 cut(s) 47
BsuTUI ATCGAT 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 190, 334, 382
CfoI GCGC 1 cut(s) 85
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 4 cut(s) 250, 395, 417, 487
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviAII CATG 3 cut(s) 35, 352, 398
CviQI GTAC 4 cut(s) 250, 395, 417, 487
DdeI CTNAG 1 cut(s) 434
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 361
Eco147I AGGCCT 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 71, 180
EcoT38I GRGCYC 1 cut(s) 363
FaeI CATG 3 cut(s) 38, 355, 401
FaiI YATR 7 cut(s) 17, 36, 93, 150, 353, 399, 533
FaqI GGGAC 1 cut(s) 89
FatI CATG 3 cut(s) 34, 351, 397
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 3 cut(s) 197, 341, 389
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 226
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 1 cut(s) 47
HhaI GCGC 1 cut(s) 85
Hin1II CATG 3 cut(s) 38, 355, 401
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 95, 501
Hpy188I TCNGA 2 cut(s) 169, 510
HpyAV CCTTC 4 cut(s) 241, 282, 349, 515
HpyCH4V TGCA 4 cut(s) 38, 107, 228, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 434
Hsp92II CATG 3 cut(s) 38, 355, 401
HspAI GCGC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 4 cut(s) 142, 267, 358, 527
LpnPI CCDG 6 cut(s) 58, 85, 167, 194, 257, 450
Lsp1109I GCAGC 1 cut(s) 212
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 517
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 5 cut(s) 182, 400, 413, 453, 487
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 116, 511
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 5 cut(s) 13, 37, 240, 397, 401
MseI TTAA 2 cut(s) 315, 541
MslI CAYNNNNRTG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 73, 182
MvaI CCWGG 2 cut(s) 73, 182
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 1 cut(s) 379
NlaIII CATG 3 cut(s) 38, 355, 401
NspI RCATGY 1 cut(s) 38
PceI AGGCCT 1 cut(s) 47
PfeI GAWTC 1 cut(s) 501
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 1 cut(s) 180
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 71, 180
PspGI CCWGG 2 cut(s) 71, 180
RsaI GTAC 4 cut(s) 251, 396, 418, 488
RsaNI GTAC 4 cut(s) 250, 395, 417, 487
RseI CAYNNNNRTG 1 cut(s) 90
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 315, 541
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 1 cut(s) 379
ScaI AGTACT 1 cut(s) 418
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 73, 182
SduI GDGCHC 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 90
Sse9I AATT 2 cut(s) 116, 511
SseBI AGGCCT 1 cut(s) 47
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 71, 180
TaqI TCGA 2 cut(s) 156, 201
TasI AATT 2 cut(s) 116, 511
TatI WGTACW 3 cut(s) 394, 416, 486
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 2 cut(s) 315, 541
Tru9I TTAA 2 cut(s) 315, 541
TseI GCWGC 1 cut(s) 225
TspDTI ATGAA 3 cut(s) 165, 368, 414
Van91I CCANNNNNTGG 1 cut(s) 271
XapI RAATTY 2 cut(s) 116, 511
XceI RCATGY 1 cut(s) 38
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.