AT3G50460

plant-type hypersensitive response

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014069
Physical Location & Seq
Forward (+)
18727666 .. 18728810
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50460.1

Sequence Viewer

Length: 618 bp
ATGCCTCTTACCGAGATTATCGCAGGGGCTGCTCTTGGACTTGCTCTCCAAATCCTTCACGAGGCCATCCAAAGAGCAAAAGATAGATCCTTAACCACAAGTTGTATCTTGGACCGTCTCGATTCTACAATCTTGAGGATCACTCCACTGATGGCTAAGGTCGAAAAGCTCAACAAAGAATCCGATGAATCTCTGAGGAAAGTCTTCGAAGATCTTAAACATCTTCTCGAGAAAGCCGTGGTTCTTGTCGAAGCTTATGCGGAGCTCAAACGCAGAAACTTACTTGGAAAATATAGGTACAAGAGAAGAATCAAAGAGTTAGAAGGTTCATTGAAATGGATGGTAGATGTTGACGTTAAAGTCAATCAATGGGCAGATATCAAAGATCTTATGGCCAAGATGTCTGAAATGAACACTAAACTTGAAAAAATCATGGGTCAACCAATAGATTGTATTATATCTGAAGATAATACTAATATGGACATAGTCGAAAGAGTTGATCCATCATTGGAAGCAAAAGCTGGATGCTCAAATAGCGATTCTAAGCCGAAGATCGATATCCACCTTCGATGGAGCAAGCAAAGCAAAGATCACGGAATTCGCTTCGTTTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPW8

Protein Analysis

205

Amino Acids

23.44

Weight (kDa)

8.66

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 142 2.6e-55 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50450 AT3G50460 AT3G50470 AT3G50480 AT3G50480
malus_domestica MD02G1163800.v1.1
prunus_persica Prupe.7G140100_v2.0.a1
rosa_laevigata RLG00000017290
rosa_multiflora Rmu_ssc0000257.1_g000011
rosa_roxburghii Rroxscaffold_2G00139110
rosa_rugosa Rorug02G0119300
rosa_samantha Rh2AG168800 Rh2BG176000 Rh2CG174400 Rh2DG174300 Rh6DG281400
rosa_wichuraiana Rw2G013210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AciI CCGC 1 cut(s) 260
AclWI GGATC 3 cut(s) 81, 146, 494
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 597
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 3 cut(s) 334, 425, 613
AluBI AGCT 4 cut(s) 169, 254, 265, 521
AluI AGCT 4 cut(s) 169, 254, 265, 521
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 3 cut(s) 81, 146, 494
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 2 cut(s) 63, 393
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 597
Asp700I GAANNNNTTC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 395
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 16
BccI CCATC 5 cut(s) 74, 145, 334, 511, 564
BceAI ACGGC 1 cut(s) 221
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BcoDI GTCTC 1 cut(s) 122
BglII AGATCT 2 cut(s) 211, 385
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 112
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 112
BmsI GCATC 1 cut(s) 515
BpiI GAAGAC 1 cut(s) 196
BplI GAGNNNNNCTC 2 cut(s) 127, 159
Bpu10I CCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 154
Bsa29I ATCGAT 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 557
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 557
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 3 cut(s) 66, 345, 530
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 185
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 2 cut(s) 65, 395
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 122
BsmBI CGTCTC 1 cut(s) 122
BsnI GGCC 2 cut(s) 65, 395
BsoBI CYCGRG 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
BspACI CCGC 1 cut(s) 260
BspANI GGCC 2 cut(s) 65, 395
BspCNI CTCAG 1 cut(s) 186
BspDI ATCGAT 1 cut(s) 555
BspPI GGATC 3 cut(s) 81, 146, 494
BspT104I TTCGAA 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
BssSI CACGAG 1 cut(s) 59
Bst2BI CACGAG 1 cut(s) 59
Bst4CI ACNGT 1 cut(s) 116
BstAPI GCANNNNNTGC 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 578
BstDEI CTNAG 3 cut(s) 156, 194, 543
BstDSI CCRYGG 1 cut(s) 237
BstENI CCTNNNNNAGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 66, 345, 530
BstKTI GATC 7 cut(s) 89, 141, 214, 388, 502, 555, 592
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
BstMWI GCNNNNNNNGC 3 cut(s) 29, 534, 582
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 3 cut(s) 86, 211, 385
BstYI RGATCY 3 cut(s) 86, 211, 385
Bsu15I ATCGAT 1 cut(s) 555
BsuRI GGCC 2 cut(s) 65, 395
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 3 cut(s) 66, 345, 530
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 578
CaiI CAGNNNCTG 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 555
Csp6I GTAC 1 cut(s) 298
CviAII CATG 1 cut(s) 433
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 3 cut(s) 156, 194, 543
DpnI GATC 7 cut(s) 88, 140, 213, 387, 501, 554, 591
DpnII GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 393
Ecl136II GAGCTC 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 267
Eco32I GATATC 2 cut(s) 379, 559
Eco47I GGWCC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 597
EcoRV GATATC 2 cut(s) 379, 559
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 1 cut(s) 436
FaiI YATR 7 cut(s) 258, 294, 392, 434, 458, 479, 485
FatI CATG 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 3 cut(s) 53, 352, 537
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 65, 395
Hin1II CATG 1 cut(s) 436
HincII GTYRAC 2 cut(s) 352, 440
HindII GTYRAC 2 cut(s) 352, 440
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 5 cut(s) 122, 179, 188, 309, 539
Hpy166II GTNNAC 2 cut(s) 352, 440
Hpy188I TCNGA 4 cut(s) 184, 195, 406, 463
Hpy188III TCNNGA 5 cut(s) 59, 119, 133, 227, 229
Hpy8I GTNNAC 2 cut(s) 352, 440
HpyAV CCTTC 3 cut(s) 65, 317, 575
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 534, 582
HpyF3I CTNAG 3 cut(s) 156, 194, 543
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 1 cut(s) 436
Kzo9I GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
LmnI GCTCC 2 cut(s) 262, 573
LpnPI CCDG 2 cut(s) 9, 507
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 515
MaeII ACGT 1 cut(s) 354
MalI GATC 7 cut(s) 88, 140, 213, 387, 501, 554, 591
MboI GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
MboII GAAGA 6 cut(s) 196, 215, 221, 318, 476, 562
MflI RGATCY 3 cut(s) 86, 211, 385
MhlI GDGCHC 1 cut(s) 267
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 597, 613
MluNI TGGCCA 1 cut(s) 395
MnlI CCTC 4 cut(s) 15, 55, 129, 189
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 92, 216, 357
MslI CAYNNNNRTG 1 cut(s) 334
Msp20I TGGCCA 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 29, 534, 582
NdeII GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
NlaIII CATG 1 cut(s) 436
NspV TTCGAA 1 cut(s) 207
PaeR7I CTCGAG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 5 cut(s) 122, 179, 188, 309, 539
PflFI GACNNNGTC 1 cut(s) 485
PkrI GCNGC 1 cut(s) 31
Psp124BI GAGCTC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 3 cut(s) 86, 211, 385
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 334
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 3 cut(s) 92, 216, 357
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 7 cut(s) 86, 138, 211, 385, 499, 552, 589
Sau96I GGNCC 1 cut(s) 112
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 162, 171, 256, 267, 299, 328, 357, 523, 567
SfaNI GCATC 1 cut(s) 515
Sfr274I CTCGAG 1 cut(s) 227
SfuI TTCGAA 1 cut(s) 207
SinI GGWCC 1 cut(s) 112
SlaI CTCGAG 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 334
SmlI CTYRAG 2 cut(s) 133, 227
SmoI CTYRAG 2 cut(s) 133, 227
Sse9I AATT 2 cut(s) 597, 613
SsiI CCGC 1 cut(s) 260
SstI GAGCTC 1 cut(s) 267
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 357
TaqI TCGA 8 cut(s) 120, 162, 207, 228, 249, 489, 555, 568
TasI AATT 2 cut(s) 597, 613
TfiI GAWTC 5 cut(s) 122, 179, 188, 309, 539
Tru1I TTAA 3 cut(s) 92, 216, 357
Tru9I TTAA 3 cut(s) 92, 216, 357
TscAI CASTG 1 cut(s) 153
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 201, 318, 425
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 59
XapI RAATTY 1 cut(s) 597
XhoI CTCGAG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.